Restriction map for pFN21AE3204
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       BamHI       BbeI        BbrPI       BcgI        
BclI        BcnI        BfrI        BglI        BlpI        
Bpu1102I    BsaAI       BsaHI       BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstUI       BstXI       BstZ17I     Bsu36I      
Cac8I       CelII       Cfr10I      ClaI        CpoI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoRV       FbaI        
FseI        FspI        HaeII       HapII       HgaI        
Hin1I       HindIII     HpaI        HpaII       KasI        
KpnI        KspI        MfeI        MluI        MroI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NgoMIV      NheI        NotI        
NruI        NspI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        SwaI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccI            3  GTCTAC               GT^[AC][GT]AC
AclI          206  AACGTT               AA^CGTT
AfaI         1212  GTAC                 GT^AC
AlwI          600  GGATC                GGATC(4/5)
ApaI         1149  GGGCCC               GGGCC^C
AseI          579  ATTAAT               AT^TAAT
AsnI          579  ATTAAT               AT^TAAT
AvrII         543  CCTAGG               C^CTAGG
BanI          839  GGCACC               G^G[CT][GA]CC
BbvCI        1332  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1200  GTATCC               GTATCC(6/5)
BglII         900  AGATCT               A^GATCT
BlnI          543  CCTAGG               C^CTAGG
BsaBI         529  GATAAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1187  GGTCTC               GGTCTC(1/5)
BseRI        1137  CTCCTC               GAGGAG(10/8)
BsgI         1357  GTGCAG               GTGCAG(16/14)
BspHI         339  TCATGA               T^CATGA
BsrBI        1369  GAGCGG               CCGCTC(-3/-3)
BsrDI         427  GCAATG               GCAATG(2/0)
BstBI         222  TTCGAA               TT^CGAA
BstEII        289  GGTCACC              G^GT[ACGT]ACC
BstPI         289  GGTCACC              G^GT[ACGT]ACC
BstYI         900  AGATCT               [GA]^GATC[CT]
CfoI          871  GCGC                 GCG^C
DraI          771  TTTAAA               TTT^AAA
DsaI          405  CCGTGG               C^C[GA][CT]GG
EcoNI        1139  CCTCAGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       289  GGTCACC              G^GT[ACGT]ACC
EcoRI        1132  GAATTC               G^AATTC
EcoT22I      1303  ATGCAT               ATGCA^T
FauI         1380  GCGGG                CCCGC(4/6)
HhaI          871  GCGC                 GCG^C
HinP1I        871  GCGC                 G^CGC
HincII        913  GTCAAC               GT[CT]^[GA]AC
HindII        913  GTCAAC               GT[CT]^[GA]AC
MaeII         207  ACGT                 A^CGT
MamI          529  GATAAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          900  AGATCT               [GA]^GATC[CT]
MslI          807  CACTCAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI          998  CATATG               CA^TATG
NsiI         1303  ATGCAT               ATGCA^T
NspV          222  TTCGAA               TT^CGAA
PpuMI          51  AGGACCC              [GA]G^G[AT]CC[CT]
PshBI         579  ATTAAT               AT^TAAT
Psp1406I      206  AACGTT               AA^CGTT
PstI          269  CTGCAG               CTGCA^G
PvuII        1375  CAGCTG               CAG^CTG
RcaI          339  TCATGA               T^CATGA
RsaI         1212  GTAC                 GT^AC
SfuI          222  TTCGAA               TT^CGAA
SspI          491  AATATT               AAT^ATT
XcmI         1082  CCATCGTCCCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         900  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

BalI          408  TGGCCA               TGG^CCA
              741  TGGCCA              
BanII        1149  GGGCCC               G[GA]GC[CT]^C
             1413  GAGCCC              
BbsI          550  GAAGAC               GAAGAC(2/6)
              777  GAAGAC              
BfaI          137  CTAG                 C^TAG
              544  CTAG                
BpuAI         550  GAAGAC               GAAGAC(2/6)
              777  GAAGAC              
BspMI          89  ACCTGC               ACCTGC(4/8)
              681  GCAGGT              
EaeI          408  TGGCCA               [CT]^GGCC[GA]
              741  TGGCCA              
MaeI          137  CTAG                 C^TAG
              544  CTAG                
MaeIII        290  GTCAC                ^GT[ACGT]AC
              794  GTGAC               
MluNI         408  TGGCCA               TGG^CCA
              741  TGGCCA              
MscI          408  TGGCCA               TGG^CCA
              741  TGGCCA              
MspA1I       1164  CCGCTG               C[AC]G^C[GT]G
             1375  CAGCTG              
NlaIII        340  CATG                 CATG^
             1410  CATG                
SfcI          269  CTGCAG               C^T[GA][CT]AG
             1245  CTACAG              
Tsp45I        290  GTCAC                ^GT[GC]AC
              794  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AciI        AcsI        AluI        AlwNI       ApoI        
AvaII       BbvI        BmyI        BpmI        BsaJI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HinfI       HphI        ItaI        Ksp632I     MboI        
MboII       MnlI        MseI        MvaI        MwoI        
NdeII       NlaIV       Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TaqI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com