################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII BamHI BbeI BbrPI BcgI BclI BcnI BfrI BglI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV FbaI FseI FspI HaeII HapII HgaI Hin1I HindIII HpaI HpaII KasI KpnI KspI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 3 GTCTAC GT^[AC][GT]AC AclI 206 AACGTT AA^CGTT AfaI 1212 GTAC GT^AC AlwI 600 GGATC GGATC(4/5) ApaI 1149 GGGCCC GGGCC^C AseI 579 ATTAAT AT^TAAT AsnI 579 ATTAAT AT^TAAT AvrII 543 CCTAGG C^CTAGG BanI 839 GGCACC G^G[CT][GA]CC BbvCI 1332 GCTGAGG CCTCAGC(-5/-2) BciVI 1200 GTATCC GTATCC(6/5) BglII 900 AGATCT A^GATCT BlnI 543 CCTAGG C^CTAGG BsaBI 529 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1187 GGTCTC GGTCTC(1/5) BseRI 1137 CTCCTC GAGGAG(10/8) BsgI 1357 GTGCAG GTGCAG(16/14) BspHI 339 TCATGA T^CATGA BsrBI 1369 GAGCGG CCGCTC(-3/-3) BsrDI 427 GCAATG GCAATG(2/0) BstBI 222 TTCGAA TT^CGAA BstEII 289 GGTCACC G^GT[ACGT]ACC BstPI 289 GGTCACC G^GT[ACGT]ACC BstYI 900 AGATCT [GA]^GATC[CT] CfoI 871 GCGC GCG^C DraI 771 TTTAAA TTT^AAA DsaI 405 CCGTGG C^C[GA][CT]GG EcoNI 1139 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 289 GGTCACC G^GT[ACGT]ACC EcoRI 1132 GAATTC G^AATTC EcoT22I 1303 ATGCAT ATGCA^T FauI 1380 GCGGG CCCGC(4/6) HhaI 871 GCGC GCG^C HinP1I 871 GCGC G^CGC HincII 913 GTCAAC GT[CT]^[GA]AC HindII 913 GTCAAC GT[CT]^[GA]AC MaeII 207 ACGT A^CGT MamI 529 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 900 AGATCT [GA]^GATC[CT] MslI 807 CACTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeI 998 CATATG CA^TATG NsiI 1303 ATGCAT ATGCA^T NspV 222 TTCGAA TT^CGAA PpuMI 51 AGGACCC [GA]G^G[AT]CC[CT] PshBI 579 ATTAAT AT^TAAT Psp1406I 206 AACGTT AA^CGTT PstI 269 CTGCAG CTGCA^G PvuII 1375 CAGCTG CAG^CTG RcaI 339 TCATGA T^CATGA RsaI 1212 GTAC GT^AC SfuI 222 TTCGAA TT^CGAA SspI 491 AATATT AAT^ATT XcmI 1082 CCATCGTCCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 900 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BalI 408 TGGCCA TGG^CCA 741 TGGCCA BanII 1149 GGGCCC G[GA]GC[CT]^C 1413 GAGCCC BbsI 550 GAAGAC GAAGAC(2/6) 777 GAAGAC BfaI 137 CTAG C^TAG 544 CTAG BpuAI 550 GAAGAC GAAGAC(2/6) 777 GAAGAC BspMI 89 ACCTGC ACCTGC(4/8) 681 GCAGGT EaeI 408 TGGCCA [CT]^GGCC[GA] 741 TGGCCA MaeI 137 CTAG C^TAG 544 CTAG MaeIII 290 GTCAC ^GT[ACGT]AC 794 GTGAC MluNI 408 TGGCCA TGG^CCA 741 TGGCCA MscI 408 TGGCCA TGG^CCA 741 TGGCCA MspA1I 1164 CCGCTG C[AC]G^C[GT]G 1375 CAGCTG NlaIII 340 CATG CATG^ 1410 CATG SfcI 269 CTGCAG C^T[GA][CT]AG 1245 CTACAG Tsp45I 290 GTCAC ^GT[GC]AC 794 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AcsI AluI AlwNI ApoI AvaII BbvI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MboI MboII MnlI MseI MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I