Restriction map for pFN21AE3204
 (Circular) (Six-base) MAPSORT of: pFN21AE3204.seq  Check: 9339  from: 1  to: 6124



pFN21AE3204  6124 bp

 With 182 enzymes: SgfI * 

                        March 28, 2012 14:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2677    2677 
   Size:       6124
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3934     278 
   Size:         53      83     186     936    2398    2468

  Fragments arranged by size: 

                2468    2398     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2009    3467    1937 
   Size:         72    1458    4594

  Fragments arranged by size: 

                4594    1458      72
 
Acc65I G'GTAC_C

Cuts at:   3445    3445 
   Size:       6124
 
AclI AA'CG_TT

Cuts at:   1563    2212    4251    4624    1563 
   Size:        649    2039     373    3063

  Fragments arranged by size: 

                3063    2039     649     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2292    2300    2621    2702    2930    3098    4187    5571 
   Size:        660       8     321      81     228     168    1089    1384

Cuts at:   5571    5839    1632 
   Size:        268    1917

  Fragments arranged by size: 

                1917    1384    1089     660     321     268     228     168
                  81       8
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5927
 
AflIII A'CryG_T

Cuts at:   1203    5023    6000    1203 
   Size:       3820     977    1327

  Fragments arranged by size: 

                3820    1327     977
 
AgeI A'CCGG_T

Cuts at:   4955    4955 
   Size:       6124
 
AhdI GACnn_n'nnGTC

Cuts at:   4853    4853 
   Size:       6124
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6124
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6124
 
AlwNI CAG_nnn'CTG

Cuts at:   2821    2936    2965    3867    5439    2821 
   Size:        115      29     902    1572    3506

  Fragments arranged by size: 

                3506    1572     902     115      29
 
ApaI G_GGCC'C

Cuts at:   3158    3158 
   Size:       6124
 
ApaLI G'TGCA_C

Cuts at:   4181    5337    4181 
   Size:       1156    4968
 
ApoI r'AATT_y

Cuts at:   1493    2159    2696    3137    3433    3659    3811    5920    1493 
   Size:        666     537     441     296     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     666     537     441     296     226     152
            
 
AseI AT'TA_AT

Cuts at:    160    1032    2585    4678     160 
   Size:        872    1553    2093    1606

  Fragments arranged by size: 

                2093    1606    1553     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6124
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3449    1870 
   Size:         73    1506    4545

  Fragments arranged by size: 

                4545    1506      73
 
AvrII C'CTAG_G

Cuts at:   2548    2548 
   Size:       6124
 
BamHI G'GATC_C

Cuts at:   3454    3821    3454 
   Size:        367    5757
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2844    3445    4900     618 
   Size:        325     217     627    1057     601    1455    1842

  Fragments arranged by size: 

                1842    1455    1057     627     601     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3158    3422    3443    1391 
   Size:       1767     264      21    4072

  Fragments arranged by size: 

                4072    1767     264      21
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2562    2789     928 
   Size:        971     663     227    4263

  Fragments arranged by size: 

                4263     971     663     227
 
BbvCI CC'TCA_GC

Cuts at:   3338    3338 
   Size:       6124
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4315    4349    1903 
   Size:         34    2378      34    3678

  Fragments arranged by size: 

                3678    2378      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3216    4016    5226    3216 
   Size:        800    1210    4114

  Fragments arranged by size: 

                4114    1210     800
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6124
 
BfrBI ATG'CAT

Cuts at:   3310    3310 
   Size:       6124
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4735     136 
   Size:        107     122      71     631     605    3063    1525

  Fragments arranged by size: 

                3063    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2905    6119    2905 
   Size:       3214    2910
 
BlpI GC'TnA_GC

Cuts at:   3541    3541 
   Size:       6124
 
Bme1580I G_kGCm'C

Cuts at:   1790    2847    3158    4185    5341    1790 
   Size:       1057     311    1027    1156    2573

  Fragments arranged by size: 

                2573    1156    1057    1027     311
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6124
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2055    3416    3855 
   Size:        416      53      83     399      24     638    1361     439

Cuts at:   3855    4813     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753    1361     958     638     439     416     399      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6124
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2123    2133    2217    2843    4784    1239 
   Size:        884      10      84     626    1941    2579

  Fragments arranged by size: 

                2579    1941     884     626      84      10
 
Bpu10I CC'TnA_GC

Cuts at:   2053    2183    2759    3338    2053 
   Size:        130     576     579    4839

  Fragments arranged by size: 

                4839     579     576     130
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4249    5114    5412    4249 
   Size:        865     298    4961

  Fragments arranged by size: 

                4961     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3198    4787     882 
   Size:        617    1699    1589    2219

  Fragments arranged by size: 

                2219    1699    1589     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3915     493 
   Size:        711    2711    2702

  Fragments arranged by size: 

                2711    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   2538    6118    2538 
   Size:       3580    2544
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3931    4313    4948     275 
   Size:         53      83     186     936    2398     382     635    1451

  Fragments arranged by size: 

                2398    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4557    4955    5229    5376    5787    4557 
   Size:        398     274     147     411    4894

  Fragments arranged by size: 

                4894     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5899

  Fragments arranged by size: 

                5899     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    3133    1186 
   Size:       1947    4177
 
BseYI C'CCAG_C

Cuts at:   2333    2371    5327    5823    2333 
   Size:         38    2956     496    2634

  Fragments arranged by size: 

                2956    2634     496      38
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    3383    1163 
   Size:        573    1647    3904

  Fragments arranged by size: 

                3904    1647     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3574    4335    5363    1540 
   Size:        275     186    1573     761    1028    2301

  Fragments arranged by size: 

                2301    1573    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   4185    4270    5341    4185 
   Size:         85    1071    4968

  Fragments arranged by size: 

                4968    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3635    3728    3635 
   Size:         93    6031
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2847    3158    3422    3443    4185    4270    5341 
   Size:        399    1057     311     264      21     742      85    1071

Cuts at:   5341    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071    1057     742     399     311     264      85
                  21
 
BspEI T'CCGG_A

Cuts at:   5787    5787 
   Size:       6124
 
BspHI T'CATG_A

Cuts at:   2344    4013    5743    2344 
   Size:       1669    1730    2725

  Fragments arranged by size: 

                2725    1730    1669
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2103    2677    3479     844 
   Size:       1259     574     802    3489

  Fragments arranged by size: 

                3489    1259     802     574
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3376    4011    1394 
   Size:        429    1553     635    3507

  Fragments arranged by size: 

                3507    1553     635     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2439    4619    4793      65 
   Size:       1183    1191    2180     174    1396

  Fragments arranged by size: 

                2180    1396    1191    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4768    4955    1700 
   Size:        110    2958     187    2869

  Fragments arranged by size: 

                2958    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6124
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6124
 
BssSI C'ACGA_G

Cuts at:   4184    5196    4184 
   Size:       1012    5112
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6124
 
BstBI TT'CG_AA

Cuts at:   2228    4928    2228 
   Size:       2700    3424
 
BstEII G'GTnAC_C

Cuts at:   1184    2294    1184 
   Size:       1110    5014
 
BstYI r'GATC_y

Cuts at:   1972    2905    3454    3821    4206    4223    5675    5761    5773 
   Size:        933     549     367     385      17    1452      86      12

Cuts at:   5773    6119    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     933     549     385     367     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2410     513 
   Size:        552    1345    4227

  Fragments arranged by size: 

                4227    1345     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5385

  Fragments arranged by size: 

                5385     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2245    3640    4434    4454    2245 
   Size:       1395     794      20    3915

  Fragments arranged by size: 

                3915    1395     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6124
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6089
 
DraI TTT'AAA

Cuts at:   2778    3428    2778 
   Size:        650    5474
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5131     809 
   Size:        507    3815    1802

  Fragments arranged by size: 

                3815    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2413    2746    3571 
   Size:         54    1425      50     275      18     583     333     825

Cuts at:   3571    4460    6089       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     825     583     333     275      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3571    1537 
   Size:        275    1759    4090

  Fragments arranged by size: 

                4090    1759     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2024    2562    3104    3177    4054    1353 
   Size:        671     538     542      73     877    3423

  Fragments arranged by size: 

                3423     877     671     542     538      73
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4711    5085    5231     387 
   Size:       1064     138    3122     374     146    1280

  Fragments arranged by size: 

                3122    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2123    2133    2217    2292    2300    2621    2702 
   Size:        393     491      10      84      75       8     321      81

Cuts at:   2702    2843    2930    3098    4187    4784    5571    5839    1239 
   Size:        141      87     168    1089     597     787     268    1524

  Fragments arranged by size: 

                1524    1089     787     597     491     393     321     268
                 168     141      87      84      81      75      10       8
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3148    3148 
   Size:       6124
 
EcoO109I rG'GnC_Cy

Cuts at:   2057    3154    3155    2057 
   Size:       1097       1    5026

  Fragments arranged by size: 

                5026    1097       1
 
EcoRI G'AATT_C

Cuts at:   3137    3433    3811    3137 
   Size:        296     378    5450

  Fragments arranged by size: 

                5450     378     296
 
FspI TGC'GCA

Cuts at:   4630    4630 
   Size:       6124
 
HaeII r_GCGC'y

Cuts at:   1678    5271    1678 
   Size:       3593    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2776    2808    3009    3041    3176 
   Size:         32     135      32    1204      32     201      32     135

Cuts at:   3176    3208    4765    4797    4839    4871    1373 
   Size:         32    1557      32      42      32    2626

  Fragments arranged by size: 

                2626    1557    1204     201     135     135      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2920    3468    3714     669 
   Size:        988     281     982     548     246    3079

  Fragments arranged by size: 

                3079     988     982     548     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       6124
 
HpaI GTT'AAC

Cuts at:   3714    3714 
   Size:       6124
 
KpnI G_GTAC'C

Cuts at:   3449    3449 
   Size:       6124
 
MfeI C'AATT_G

Cuts at:   3723    3723 
   Size:       6124
 
MluI A'CGCG_T

Cuts at:   6000    6000 
   Size:       6124
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    5238    5422    1305 
   Size:       3933     184    2007

  Fragments arranged by size: 

                3933    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2415    2748      10 
   Size:         54    1425     343     583     333    3386

  Fragments arranged by size: 

                3386    1425     583     343     333      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2816    4082    4441    4600     518 
   Size:        648    1650    1266     359     159    2042

  Fragments arranged by size: 

                2042    1650    1266     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3171    3382    3540    4217    5365    5610    1606 
   Size:       1565     211     158     677    1148     245    2120

  Fragments arranged by size: 

                2120    1565    1148     677     245     211     158
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5572
 
NdeI CA'TA_TG

Cuts at:    387    3004     387 
   Size:       2617    3507
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6124
 
NotI GC'GGCC_GC

Cuts at:   3571    3571 
   Size:       6124
 
NsiI A_TGCA'T

Cuts at:   3312    3312 
   Size:       6124
 
NspI r_CATG'y

Cuts at:   1132    1229    3482    5027    1132 
   Size:         97    2253    1545    2229

  Fragments arranged by size: 

                2253    2229    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6124
 
PciI A'CATG_T

Cuts at:   5023    5023 
   Size:       6124
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6124
 
PfoI T'CCnGG_A

Cuts at:   2100    2100 
   Size:       6124
 
PmeI GTTT'AAAC

Cuts at:   3428    3428 
   Size:       6124
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4193    4225    5706    5738    4193 
   Size:         32    1481      32    4579

  Fragments arranged by size: 

                4579    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2057    2057 
   Size:       6124
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6124
 
PsiI TTA'TAA

Cuts at:   3694    3694 
   Size:       6124
 
PspOMI G'GGCC_C

Cuts at:   3154    3154 
   Size:       6124
 
PstI C_TGCA'G

Cuts at:    830    2278    3476     830 
   Size:       1448    1198    3478

  Fragments arranged by size: 

                3478    1448    1198
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6124
 
PvuII CAG'CTG

Cuts at:   3382    3382 
   Size:       6124
 
SalI G'TCGA_C

Cuts at:   1936    3466    1936 
   Size:       1530    4594
 
SbfI CC_TGCA'GG

Cuts at:   3476    3476 
   Size:       6124
 
ScaI AGT'ACT

Cuts at:   4372    6084    4372 
   Size:       1712    4412
 
SfcI C'TryA_G

Cuts at:    826    1045    2274    3250    3472    4607    5288    5479     826 
   Size:        219    1229     976     222    1135     681     191    1471

  Fragments arranged by size: 

                1471    1229    1135     976     681     222     219     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6124
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6124
 
SmaI CCC'GGG

Cuts at:   1872    3451    1872 
   Size:       1579    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    4228    5129    5391     820 
   Size:        197     926    2285     901     262    1553

  Fragments arranged by size: 

                2285    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3915     493 
   Size:       3422    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6124
 
SphI G_CATG'C

Cuts at:   1132    3482    1132 
   Size:       2350    3774
 
SspI AAT'ATT

Cuts at:      5      52    2498    4048       5 
   Size:         47    2446    1550    2081

  Fragments arranged by size: 

                2446    2081    1550      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6124
 
StyI C'CwwG_G

Cuts at:    513    1065    2548    2749    2865    3563     513 
   Size:        552    1483     201     116     698    3074

  Fragments arranged by size: 

                3074    1483     698     552     201     116
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3119    4151    4168    4321    4506    4951     325 
   Size:       1255    1539    1032      17     153     185     445    1498

  Fragments arranged by size: 

                1539    1498    1255    1032     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3216    4370    6082 
   Size:        274      80      33      51    1443    1239    1154    1712

Cuts at:   6082      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1239    1154     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2463    2495    6084    6116    2463 
   Size:         32    3589      32    2471

  Fragments arranged by size: 

                3589    2471      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6124
 
XbaI T'CTAG_A

Cuts at:   3460    3460 
   Size:       6124
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3094    3094 
   Size:       6124
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6124
 
XmaI C'CCGG_G

Cuts at:   1870    3449    1870 
   Size:       1579    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4253    4253 
   Size:       6124
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3932     276 
   Size:         53      83     186     936    2398    2468

  Fragments arranged by size: 

                2468    2398     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI
     NotI     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI    PshAI     PsiI   PspOMI     PstI     PvuI    PvuII
     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     AscI     BaeI     BbeI     BplI    BsiWI    BsmBI
    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoRV     FalI     FseI
    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     PacI     PmlI
    PspXI     PsrI    RsrII     SacI    SacII    SanDI     SapI    SexAI
     SfiI     SfoI     SrfI     SwaI
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