(Circular) (Six-base) MAPSORT of: pFN21AE3204.seq Check: 9339 from: 1 to: 6124 pFN21AE3204 6124 bp With 182 enzymes: SgfI * March 28, 2012 14:20 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2677 2677 Size: 6124 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3934 278 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2009 3467 1937 Size: 72 1458 4594 Fragments arranged by size: 4594 1458 72 Acc65I G'GTAC_C Cuts at: 3445 3445 Size: 6124 AclI AA'CG_TT Cuts at: 1563 2212 4251 4624 1563 Size: 649 2039 373 3063 Fragments arranged by size: 3063 2039 649 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2292 2300 2621 2702 2930 3098 4187 5571 Size: 660 8 321 81 228 168 1089 1384 Cuts at: 5571 5839 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1089 660 321 268 228 168 81 8 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5927 AflIII A'CryG_T Cuts at: 1203 5023 6000 1203 Size: 3820 977 1327 Fragments arranged by size: 3820 1327 977 AgeI A'CCGG_T Cuts at: 4955 4955 Size: 6124 AhdI GACnn_n'nnGTC Cuts at: 4853 4853 Size: 6124 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6124 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6124 AlwNI CAG_nnn'CTG Cuts at: 2821 2936 2965 3867 5439 2821 Size: 115 29 902 1572 3506 Fragments arranged by size: 3506 1572 902 115 29 ApaI G_GGCC'C Cuts at: 3158 3158 Size: 6124 ApaLI G'TGCA_C Cuts at: 4181 5337 4181 Size: 1156 4968 ApoI r'AATT_y Cuts at: 1493 2159 2696 3137 3433 3659 3811 5920 1493 Size: 666 537 441 296 226 152 2109 1697 Fragments arranged by size: 2109 1697 666 537 441 296 226 152 AseI AT'TA_AT Cuts at: 160 1032 2585 4678 160 Size: 872 1553 2093 1606 Fragments arranged by size: 2093 1606 1553 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 AvaI C'yCGr_G Cuts at: 1870 1943 3449 1870 Size: 73 1506 4545 Fragments arranged by size: 4545 1506 73 AvrII C'CTAG_G Cuts at: 2548 2548 Size: 6124 BamHI G'GATC_C Cuts at: 3454 3821 3454 Size: 367 5757 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2844 3445 4900 618 Size: 325 217 627 1057 601 1455 1842 Fragments arranged by size: 1842 1455 1057 627 601 325 217 BanII G_rGCy'C Cuts at: 1391 3158 3422 3443 1391 Size: 1767 264 21 4072 Fragments arranged by size: 4072 1767 264 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2562 2789 928 Size: 971 663 227 4263 Fragments arranged by size: 4263 971 663 227 BbvCI CC'TCA_GC Cuts at: 3338 3338 Size: 6124 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4315 4349 1903 Size: 34 2378 34 3678 Fragments arranged by size: 3678 2378 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3216 4016 5226 3216 Size: 800 1210 4114 Fragments arranged by size: 4114 1210 800 BclI T'GATC_A Cuts at: 1548 1548 Size: 6124 BfrBI ATG'CAT Cuts at: 3310 3310 Size: 6124 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4735 136 Size: 107 122 71 631 605 3063 1525 Fragments arranged by size: 3063 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2905 6119 2905 Size: 3214 2910 BlpI GC'TnA_GC Cuts at: 3541 3541 Size: 6124 Bme1580I G_kGCm'C Cuts at: 1790 2847 3158 4185 5341 1790 Size: 1057 311 1027 1156 2573 Fragments arranged by size: 2573 1156 1057 1027 311 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6124 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2055 3416 3855 Size: 416 53 83 399 24 638 1361 439 Cuts at: 3855 4813 442 Size: 958 1753 Fragments arranged by size: 1753 1361 958 638 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6124 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2123 2133 2217 2843 4784 1239 Size: 884 10 84 626 1941 2579 Fragments arranged by size: 2579 1941 884 626 84 10 Bpu10I CC'TnA_GC Cuts at: 2053 2183 2759 3338 2053 Size: 130 576 579 4839 Fragments arranged by size: 4839 579 576 130 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4249 5114 5412 4249 Size: 865 298 4961 Fragments arranged by size: 4961 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3198 4787 882 Size: 617 1699 1589 2219 Fragments arranged by size: 2219 1699 1589 617 BsaAI yAC'GTr Cuts at: 493 1204 3915 493 Size: 711 2711 2702 Fragments arranged by size: 2711 2702 711 BsaBI GATnn'nnATC Cuts at: 2538 6118 2538 Size: 3580 2544 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3931 4313 4948 275 Size: 53 83 186 936 2398 382 635 1451 Fragments arranged by size: 2398 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4557 4955 5229 5376 5787 4557 Size: 398 274 147 411 4894 Fragments arranged by size: 4894 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5899 Fragments arranged by size: 5899 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3133 1186 Size: 1947 4177 BseYI C'CCAG_C Cuts at: 2333 2371 5327 5823 2333 Size: 38 2956 496 2634 Fragments arranged by size: 2956 2634 496 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3383 1163 Size: 573 1647 3904 Fragments arranged by size: 3904 1647 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3574 4335 5363 1540 Size: 275 186 1573 761 1028 2301 Fragments arranged by size: 2301 1573 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4185 4270 5341 4185 Size: 85 1071 4968 Fragments arranged by size: 4968 1071 85 BsmI GAATG_Cn' Cuts at: 3635 3728 3635 Size: 93 6031 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2847 3158 3422 3443 4185 4270 5341 Size: 399 1057 311 264 21 742 85 1071 Cuts at: 5341 1391 Size: 2174 Fragments arranged by size: 2174 1071 1057 742 399 311 264 85 21 BspEI T'CCGG_A Cuts at: 5787 5787 Size: 6124 BspHI T'CATG_A Cuts at: 2344 4013 5743 2344 Size: 1669 1730 2725 Fragments arranged by size: 2725 1730 1669 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2103 2677 3479 844 Size: 1259 574 802 3489 Fragments arranged by size: 3489 1259 802 574 BsrBI CCG'CTC Cuts at: 1394 1823 3376 4011 1394 Size: 429 1553 635 3507 Fragments arranged by size: 3507 1553 635 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2439 4619 4793 65 Size: 1183 1191 2180 174 1396 Fragments arranged by size: 2180 1396 1191 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4768 4955 1700 Size: 110 2958 187 2869 Fragments arranged by size: 2958 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6124 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6124 BssSI C'ACGA_G Cuts at: 4184 5196 4184 Size: 1012 5112 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6124 BstBI TT'CG_AA Cuts at: 2228 4928 2228 Size: 2700 3424 BstEII G'GTnAC_C Cuts at: 1184 2294 1184 Size: 1110 5014 BstYI r'GATC_y Cuts at: 1972 2905 3454 3821 4206 4223 5675 5761 5773 Size: 933 549 367 385 17 1452 86 12 Cuts at: 5773 6119 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 933 549 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2410 513 Size: 552 1345 4227 Fragments arranged by size: 4227 1345 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5385 Fragments arranged by size: 5385 683 56 BtsI GCAGTG_nn' Cuts at: 2245 3640 4434 4454 2245 Size: 1395 794 20 3915 Fragments arranged by size: 3915 1395 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6124 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6089 DraI TTT'AAA Cuts at: 2778 3428 2778 Size: 650 5474 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5131 809 Size: 507 3815 1802 Fragments arranged by size: 3815 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2413 2746 3571 Size: 54 1425 50 275 18 583 333 825 Cuts at: 3571 4460 6089 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 825 583 333 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3571 1537 Size: 275 1759 4090 Fragments arranged by size: 4090 1759 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2024 2562 3104 3177 4054 1353 Size: 671 538 542 73 877 3423 Fragments arranged by size: 3423 877 671 542 538 73 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4711 5085 5231 387 Size: 1064 138 3122 374 146 1280 Fragments arranged by size: 3122 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2133 2217 2292 2300 2621 2702 Size: 393 491 10 84 75 8 321 81 Cuts at: 2702 2843 2930 3098 4187 4784 5571 5839 1239 Size: 141 87 168 1089 597 787 268 1524 Fragments arranged by size: 1524 1089 787 597 491 393 321 268 168 141 87 84 81 75 10 8 EcoNI CCTnn'n_nnAGG Cuts at: 3148 3148 Size: 6124 EcoO109I rG'GnC_Cy Cuts at: 2057 3154 3155 2057 Size: 1097 1 5026 Fragments arranged by size: 5026 1097 1 EcoRI G'AATT_C Cuts at: 3137 3433 3811 3137 Size: 296 378 5450 Fragments arranged by size: 5450 378 296 FspI TGC'GCA Cuts at: 4630 4630 Size: 6124 HaeII r_GCGC'y Cuts at: 1678 5271 1678 Size: 3593 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2776 2808 3009 3041 3176 Size: 32 135 32 1204 32 201 32 135 Cuts at: 3176 3208 4765 4797 4839 4871 1373 Size: 32 1557 32 42 32 2626 Fragments arranged by size: 2626 1557 1204 201 135 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2920 3468 3714 669 Size: 988 281 982 548 246 3079 Fragments arranged by size: 3079 988 982 548 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6124 HpaI GTT'AAC Cuts at: 3714 3714 Size: 6124 KpnI G_GTAC'C Cuts at: 3449 3449 Size: 6124 MfeI C'AATT_G Cuts at: 3723 3723 Size: 6124 MluI A'CGCG_T Cuts at: 6000 6000 Size: 6124 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5238 5422 1305 Size: 3933 184 2007 Fragments arranged by size: 3933 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2415 2748 10 Size: 54 1425 343 583 333 3386 Fragments arranged by size: 3386 1425 583 343 333 54 MslI CAynn'nnrTG Cuts at: 518 1166 2816 4082 4441 4600 518 Size: 648 1650 1266 359 159 2042 Fragments arranged by size: 2042 1650 1266 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3171 3382 3540 4217 5365 5610 1606 Size: 1565 211 158 677 1148 245 2120 Fragments arranged by size: 2120 1565 1148 677 245 211 158 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5572 NdeI CA'TA_TG Cuts at: 387 3004 387 Size: 2617 3507 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6124 NotI GC'GGCC_GC Cuts at: 3571 3571 Size: 6124 NsiI A_TGCA'T Cuts at: 3312 3312 Size: 6124 NspI r_CATG'y Cuts at: 1132 1229 3482 5027 1132 Size: 97 2253 1545 2229 Fragments arranged by size: 2253 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6124 PciI A'CATG_T Cuts at: 5023 5023 Size: 6124 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6124 PfoI T'CCnGG_A Cuts at: 2100 2100 Size: 6124 PmeI GTTT'AAAC Cuts at: 3428 3428 Size: 6124 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4193 4225 5706 5738 4193 Size: 32 1481 32 4579 Fragments arranged by size: 4579 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2057 2057 Size: 6124 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6124 PsiI TTA'TAA Cuts at: 3694 3694 Size: 6124 PspOMI G'GGCC_C Cuts at: 3154 3154 Size: 6124 PstI C_TGCA'G Cuts at: 830 2278 3476 830 Size: 1448 1198 3478 Fragments arranged by size: 3478 1448 1198 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6124 PvuII CAG'CTG Cuts at: 3382 3382 Size: 6124 SalI G'TCGA_C Cuts at: 1936 3466 1936 Size: 1530 4594 SbfI CC_TGCA'GG Cuts at: 3476 3476 Size: 6124 ScaI AGT'ACT Cuts at: 4372 6084 4372 Size: 1712 4412 SfcI C'TryA_G Cuts at: 826 1045 2274 3250 3472 4607 5288 5479 826 Size: 219 1229 976 222 1135 681 191 1471 Fragments arranged by size: 1471 1229 1135 976 681 222 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6124 SmaI CCC'GGG Cuts at: 1872 3451 1872 Size: 1579 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4228 5129 5391 820 Size: 197 926 2285 901 262 1553 Fragments arranged by size: 2285 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3915 493 Size: 3422 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6124 SphI G_CATG'C Cuts at: 1132 3482 1132 Size: 2350 3774 SspI AAT'ATT Cuts at: 5 52 2498 4048 5 Size: 47 2446 1550 2081 Fragments arranged by size: 2446 2081 1550 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6124 StyI C'CwwG_G Cuts at: 513 1065 2548 2749 2865 3563 513 Size: 552 1483 201 116 698 3074 Fragments arranged by size: 3074 1483 698 552 201 116 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3119 4151 4168 4321 4506 4951 325 Size: 1255 1539 1032 17 153 185 445 1498 Fragments arranged by size: 1539 1498 1255 1032 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3216 4370 6082 Size: 274 80 33 51 1443 1239 1154 1712 Cuts at: 6082 96 Size: 138 Fragments arranged by size: 1712 1443 1239 1154 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2463 2495 6084 6116 2463 Size: 32 3589 32 2471 Fragments arranged by size: 3589 2471 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6124 XbaI T'CTAG_A Cuts at: 3460 3460 Size: 6124 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3094 3094 Size: 6124 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6124 XmaI C'CCGG_G Cuts at: 1870 3449 1870 Size: 1579 4545 XmnI GAAnn'nnTTC Cuts at: 4253 4253 Size: 6124 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3932 276 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI