################################## # NO CUT ENZYMES ( 180 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI Fnu4HI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MseI MspA1I MspI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I TseI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 360 CGCG CG^CG AciI 73 GCGG CCGC(-3/-1) AcsI 389 AAATTT [GA]^AATT[CT] AfaI 292 GTAC GT^AC AhdI 153 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AluI 123 AGCT AG^CT ApoI 389 AAATTT [GA]^AATT[CT] AspEI 153 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 39 GTGCTC G[AT]GC[AT]^C AvaII 15 GGTCC G^G[AT]CC BalI 8 TGGCCA TGG^CCA BamHI 77 GGATCC G^GATCC BbsI 380 GTCTTC GAAGAC(2/6) BmyI 39 GTGCTC G[AGT]GC[ACT]^C BpuAI 380 GTCTTC GAAGAC(2/6) BsgI 189 CTGCAC GTGCAG(16/14) BsiHKAI 39 GTGCTC G[AT]GC[AT]^C BsmI 173 GCATTC GAATGC(1/-1) Bsp1286I 39 GTGCTC G[AGT]GC[ACT]^C BsrI 155 CCAGT ACTGG(1/-1) BstF5I 416 GGATG GGATG(2/0) BstUI 360 CGCG CG^CG CfoI 361 GCGC GCG^C Cfr13I 15 GGTCC G^G[ACGT]CC DdeI 221 CTCAG C^T[ACGT]AG EaeI 8 TGGCCA [CT]^GGCC[GA] Eam1105I 153 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 375 GAAGAG CTCTTC(1/4) FauI 73 GCGGG CCCGC(4/6) FokI 416 GGATG GGATG(9/13) HhaI 361 GCGC GCG^C HinP1I 361 GCGC G^CGC HinfI 378 GAGTC G^A[ACGT]TC HphI 273 GGTGA GGTGA(8/7) Ksp632I 375 GAAGAG CTCTTC(1/4) MaeIII 151 GTGAC ^GT[ACGT]AC MluNI 8 TGGCCA TGG^CCA MscI 8 TGGCCA TGG^CCA MslI 136 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 360 CGCG CG^CG MwoI 352 GCAGGCAACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 11 CCATGG C^CATGG NlaIV 77 GGATCC GG[ACGT]^[ACGT]CC PleI 378 GAGTC GAGTC(4/5) PstI 138 CTGCAG CTGCA^G RsaI 292 GTAC GT^AC Sau96I 15 GGTCC G^G[ACGT]CC ScaI 291 AGTACT AGT^ACT SfaNI 108 GATGC GCATC(5/9) SfcI 138 CTGCAG C^T[GA][CT]AG Tsp45I 151 GTGAC ^GT[GC]AC Tsp509I 390 AATT ^AATT ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BsiYI 68 CCTTGGCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 342 CCAAACCACGG BslI 68 CCTTGGCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 342 CCAAACCACGG BssKI 54 CCAGG ^CC[ACGT]GG 81 CCTGG BstNI 54 CCAGG CC^[AT]GG 81 CCTGG BstYI 77 GGATCC [GA]^GATC[CT] 117 AGATCC Cac8I 49 GCAGGC GC[ACGT]^[ACGT]GC 352 GCAGGC DpnII 78 GATC ^GATC 118 GATC EcoRII 54 CCAGG ^CC[AT]GG 81 CCTGG EcoT14I 11 CCATGG C^C[AT][AT]GG 68 CCTTGG HaeIII 9 GGCC GG^CC 52 GGCC MboI 78 GATC ^GATC 118 GATC MflI 77 GGATCC [GA]^GATC[CT] 117 AGATCC MvaI 54 CCAGG CC^[AT]GG 81 CCTGG NdeII 78 GATC ^GATC 118 GATC Sau3AI 78 GATC ^GATC 118 GATC ScrFI 54 CCAGG CC^[ACGT]GG 81 CCTGG StyI 11 CCATGG C^C[AT][AT]GG 68 CCTTGG XhoII 77 GGATCC [GA]^GATC[CT] 117 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### AlwI BsaJI BsmAI DsaI MboII MnlI NlaIII TspRI