################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflIII AgeI AhdI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BbrPI BbvCI BciVI BclI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BsrBI BsrI Bst1107I BstBI BstXI BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I EcoNI EcoRI EcoRV FbaI FseI HgaI HincII HindII HindIII HpaI KpnI MaeII MamI MfeI MluI MluNI MroI MscI MslI MunI NcoI NdeI NheI NotI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tsp509I Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccI 361 GTCTAC GT^[AC][GT]AC AcyI 223 GGCGCC G[GA]^CG[CT]C AflII 877 CTTAAG C^TTAAG Aor51HI 886 AGCGCT AGC^GCT ApaI 230 GGGCCC GGGCC^C AscI 52 GGCGCGCC GG^CGCGCC AviII 270 TGCGCA TGC^GCA BamHI 200 GGATCC G^GATCC BanI 223 GGCGCC G^G[CT][GA]CC BbeI 223 GGCGCC GGCGC^C BbsI 756 GAAGAC GAAGAC(2/6) BcgI 1139 CGATGACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 658 CTAG C^TAG BfrI 877 CTTAAG C^TTAAG BpmI 307 CTGGAG CTGGAG(16/14) BpuAI 756 GAAGAC GAAGAC(2/6) BsaHI 223 GGCGCC G[GA]^CG[CT]C BsaI 355 GAGACC GGTCTC(1/5) BseRI 91 GAGGAG GAGGAG(10/8) BsmI 1090 GCATTC GAATGC(1/-1) BsrDI 861 CATTGC GCAATG(2/0) BssHII 53 GCGCGC G^CGCGC BssSI 163 CTCGTG CACGAG(-5/-1) BstAPI 726 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 676 GGTCACC G^GT[ACGT]ACC BstPI 676 GGTCACC G^GT[ACGT]ACC DraIII 622 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 185 CGGCCG C^GGCCG EclXI 185 CGGCCG C^GGCCG Eco47III 886 AGCGCT AGC^GCT Eco52I 185 CGGCCG C^GGCCG EcoO65I 676 GGTCACC G^GT[ACGT]ACC FspI 270 TGCGCA TGC^GCA Hin1I 223 GGCGCC G[GA]^CG[CT]C KasI 223 GGCGCC G^GCGCC KspI 234 CCGCGG CCGC^GG MaeI 658 CTAG C^TAG MaeIII 677 GTCAC ^GT[ACGT]AC MseI 878 TTAA T^TAA NarI 223 GGCGCC GG^CGCC NruI 931 TCGCGA TCG^CGA PflMI 900 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 209 GGGTCCC [GA]G^G[AT]CC[CT] SacI 148 GAGCTC GAGCT^C SacII 234 CCGCGG CCGC^GG SapI 937 GAAGAGC GCTCTTC(1/4) ScaI 950 AGTACT AGT^ACT SexAI 672 ACCTGGT A^CC[AT]GGT SfaNI 654 GATGC GCATC(5/9) SfoI 223 GGCGCC GGC^GCC StuI 553 AGGCCT AGG^CCT TfiI 1063 GAATC G^A[AT]TC Tru9I 878 TTAA T^TAA Tsp45I 677 GTCAC ^GT[GC]AC Van91I 900 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### Alw44I 333 GTGCAC G^TGCAC 870 GTGCAC AlwNI 727 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 901 CAGATCCTG AocI 596 CCTCAGG CC^T[ACGT]AGG 716 CCTCAGG ApaLI 333 GTGCAC G^TGCAC 870 GTGCAC AvaII 127 GGTCC G^G[AT]CC 210 GGTCC BsmAI 276 GAGAC GTCTC(1/5) 355 GAGAC Bsp1407I 347 TGTACA T^GTACA 833 TGTACA BspMI 522 ACCTGC ACCTGC(4/8) 914 GCAGGT BsrGI 347 TGTACA T^GTACA 833 TGTACA BstYI 200 GGATCC [GA]^GATC[CT] 902 AGATCC Bsu36I 596 CCTCAGG CC^T[ACGT]AGG 716 CCTCAGG DdeI 597 CTCAG C^T[ACGT]AG 717 CTCAG DpnII 201 GATC ^GATC 903 GATC DsaI 234 CCGCGG C^C[GA][CT]GG 462 CCACGG Eco81I 596 CCTCAGG CC^T[ACGT]AGG 716 CCTCAGG EcoT14I 773 CCAAGG C^C[AT][AT]GG 854 CCAAGG EcoT22I 385 ATGCAT ATGCA^T 1088 ATGCAT HphI 638 TCACC GGTGA(8/7) 678 TCACC MboI 201 GATC ^GATC 903 GATC MflI 200 GGATCC [GA]^GATC[CT] 902 AGATCC NaeI 187 GCCGGC GCC^GGC 220 GCCGGC NdeII 201 GATC ^GATC 903 GATC NgoMIV 187 GCCGGC G^CCGGC 220 GCCGGC NsiI 385 ATGCAT ATGCA^T 1088 ATGCAT NspI 344 GCATGT [GA]CATG^[CT] 986 ACATGC PstI 559 CTGCAG CTGCA^G 733 CTGCAG PvuII 7 CAGCTG CAG^CTG 979 CAGCTG Sau3AI 201 GATC ^GATC 903 GATC SfcI 559 CTGCAG C^T[GA][CT]AG 733 CTGCAG SmaI 204 CCCGGG CCC^GGG 213 CCCGGG SmlI 151 CTCAAG C^T[CT][GA]AG 877 CTTAAG SspBI 347 TGTACA T^GTACA 833 TGTACA StyI 773 CCAAGG C^C[AT][AT]GG 854 CCAAGG TaqI 743 TCGA T^CGA 1045 TCGA TspRI 624 CACTG CA[GC]TG[ACGT][ACGT]^ 1145 CAGTG TthHB8I 743 TCGA T^CGA 1045 TCGA XhoII 200 GGATCC [GA]^GATC[CT] 902 AGATCC XmaCI 204 CCCGGG C^CCGGG 213 CCCGGG XmaI 204 CCCGGG C^CCGGG 213 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaI BanII BbvI BcnI BmyI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI RsaI Sau96I ScrFI TseI