(Circular) (Six-base) MAPSORT of: pFN21AB5872.seq Check: 4821 from: 1 to: 5857 pFN21AB5872 5857 bp With 182 enzymes: SgfI * July 6, 2012 17:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2536 2910 2536 Size: 374 5483 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3667 278 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2367 3200 1937 Size: 430 833 4594 Fragments arranged by size: 4594 833 430 Acc65I G'GTAC_C Cuts at: 3178 3178 Size: 5857 AclI AA'CG_TT Cuts at: 1563 3984 4357 1563 Size: 2421 373 3063 Fragments arranged by size: 3063 2421 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2345 2780 3920 5304 5572 1632 Size: 713 435 1140 1384 268 1917 Fragments arranged by size: 1917 1384 1140 713 435 268 AfeI AGC'GCT Cuts at: 2893 2893 Size: 5857 AflII C'TTAA_G Cuts at: 820 1017 2882 820 Size: 197 1865 3795 Fragments arranged by size: 3795 1865 197 AflIII A'CryG_T Cuts at: 1203 4756 5733 1203 Size: 3553 977 1327 Fragments arranged by size: 3553 1327 977 AgeI A'CCGG_T Cuts at: 4688 4688 Size: 5857 AhdI GACnn_n'nnGTC Cuts at: 4586 4586 Size: 5857 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5857 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5857 AlwNI CAG_nnn'CTG Cuts at: 2737 2911 3600 5172 2737 Size: 174 689 1572 3422 Fragments arranged by size: 3422 1572 689 174 ApaI G_GGCC'C Cuts at: 2239 2239 Size: 5857 ApaLI G'TGCA_C Cuts at: 2338 2875 3914 5070 2338 Size: 537 1039 1156 3125 Fragments arranged by size: 3125 1156 1039 537 ApoI r'AATT_y Cuts at: 1493 3166 3392 3544 5653 1493 Size: 1673 226 152 2109 1697 Fragments arranged by size: 2109 1697 1673 226 152 AscI GG'CGCG_CC Cuts at: 2058 2058 Size: 5857 AseI AT'TA_AT Cuts at: 160 1032 4411 160 Size: 872 3379 1606 Fragments arranged by size: 3379 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 AvaI C'yCGr_G Cuts at: 1870 1943 2135 2209 2218 2258 2407 3182 1870 Size: 73 192 74 9 40 149 775 4545 Fragments arranged by size: 4545 775 192 149 74 73 40 9 BamHI G'GATC_C Cuts at: 2205 3187 3554 2205 Size: 982 367 4508 Fragments arranged by size: 4508 982 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2228 3178 4633 618 Size: 325 217 627 441 950 1455 1842 Fragments arranged by size: 1842 1455 950 627 441 325 217 BanII G_rGCy'C Cuts at: 1391 2157 2239 2310 3176 1391 Size: 766 82 71 866 4072 Fragments arranged by size: 4072 866 766 82 71 BbeI G_GCGC'C Cuts at: 2232 2232 Size: 5857 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2768 928 Size: 971 869 4017 Fragments arranged by size: 4017 971 869 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3133 3167 4048 4082 1903 Size: 34 50 34 1112 34 881 34 3678 Fragments arranged by size: 3678 1112 881 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3749 4959 3749 Size: 1210 4647 BclI T'GATC_A Cuts at: 1548 1548 Size: 5857 BfrBI ATG'CAT Cuts at: 2392 3095 2392 Size: 703 5154 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4468 136 Size: 107 122 71 631 605 2796 1525 Fragments arranged by size: 2796 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5852 5852 Size: 5857 BlpI GC'TnA_GC Cuts at: 3274 3274 Size: 5857 Bme1580I G_kGCm'C Cuts at: 1790 2239 2342 2718 2879 3918 5074 1790 Size: 449 103 376 161 1039 1156 2573 Fragments arranged by size: 2573 1156 1039 449 376 161 103 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5857 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3588 4546 442 Size: 416 53 83 399 24 2171 958 1753 Fragments arranged by size: 2171 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5857 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2333 4517 1239 Size: 1094 2184 2579 Fragments arranged by size: 2579 2184 1094 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2141 3982 4847 5145 2141 Size: 1841 865 298 2853 Fragments arranged by size: 2853 1841 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2354 4520 882 Size: 617 855 2166 2219 Fragments arranged by size: 2219 2166 855 617 BsaAI yAC'GTr Cuts at: 493 1204 3648 493 Size: 711 2444 2702 Fragments arranged by size: 2702 2444 711 BsaBI GATnn'nnATC Cuts at: 5851 5851 Size: 5857 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2229 3664 4046 4681 Size: 53 83 186 936 696 1435 382 635 Cuts at: 4681 275 Size: 1451 Fragments arranged by size: 1451 1435 936 696 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4290 4688 4962 5109 5520 4290 Size: 398 274 147 411 4627 Fragments arranged by size: 4627 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5632 Fragments arranged by size: 5632 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2111 1186 Size: 925 4932 BseYI C'CCAG_C Cuts at: 2427 2686 3127 5060 5556 2427 Size: 259 441 1933 496 2728 Fragments arranged by size: 2728 1933 496 441 259 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2429 2508 2972 1163 Size: 573 693 79 464 4048 Fragments arranged by size: 4048 693 573 464 79 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2193 2752 3050 3307 4068 5096 Size: 275 186 192 559 298 257 761 1028 Cuts at: 5096 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 559 298 275 257 192 186 BsiHKAI G_wGCw'C Cuts at: 2157 2342 2879 3156 3918 4003 5074 2157 Size: 185 537 277 762 85 1071 2940 Fragments arranged by size: 2940 1071 762 537 277 185 85 BsmI GAATG_Cn' Cuts at: 3095 3368 3461 3095 Size: 273 93 5491 Fragments arranged by size: 5491 273 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2157 2239 2310 2342 2718 2879 3156 Size: 399 367 82 71 32 376 161 277 Cuts at: 3156 3176 3918 4003 5074 1391 Size: 20 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 376 367 277 161 85 82 71 32 20 BspEI T'CCGG_A Cuts at: 5520 5520 Size: 5857 BspHI T'CATG_A Cuts at: 3746 5476 3746 Size: 1730 4127 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2536 2910 3212 844 Size: 1692 374 302 3489 Fragments arranged by size: 3489 1692 374 302 BsrBI CCG'CTC Cuts at: 1394 1823 3744 1394 Size: 429 1921 3507 Fragments arranged by size: 3507 1921 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2865 4352 4526 65 Size: 1183 1617 1487 174 1396 Fragments arranged by size: 1617 1487 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2129 2192 2225 4501 4688 1700 Size: 110 319 63 33 2276 187 2869 Fragments arranged by size: 2869 2276 319 187 110 63 33 BsrGI T'GTAC_A Cuts at: 96 2352 2838 96 Size: 2256 486 3115 Fragments arranged by size: 3115 2256 486 BssHII G'CGCG_C Cuts at: 1925 2058 1925 Size: 133 5724 BssSI C'ACGA_G Cuts at: 2168 3917 4929 2168 Size: 1749 1012 3096 Fragments arranged by size: 3096 1749 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2737 794 Size: 1943 3914 BstBI TT'CG_AA Cuts at: 4661 4661 Size: 5857 BstEII G'GTnAC_C Cuts at: 1184 2681 1184 Size: 1497 4360 BstYI r'GATC_y Cuts at: 1972 2205 2907 3187 3554 3939 3956 5408 5494 Size: 233 702 280 367 385 17 1452 86 Cuts at: 5494 5506 5852 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 702 385 367 346 280 233 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2602 2722 2602 Size: 120 5737 BtgI C'CryG_G Cuts at: 513 1065 2239 2467 513 Size: 552 1174 228 3903 Fragments arranged by size: 3903 1174 552 228 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5118 Fragments arranged by size: 5118 683 56 BtsI GCAGTG_nn' Cuts at: 3373 4167 4187 3373 Size: 794 20 5043 Fragments arranged by size: 5043 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5857 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5822 DraI TTT'AAA Cuts at: 3161 3161 Size: 5857 DraIII CAC_nnn'GTG Cuts at: 2632 2632 Size: 5857 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4864 809 Size: 507 3548 1802 Fragments arranged by size: 3548 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 2190 2706 Size: 54 1425 50 275 18 318 42 516 Cuts at: 2706 3304 4193 5822 8 Size: 598 889 1629 43 Fragments arranged by size: 1629 1425 889 598 516 318 275 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 2190 3304 1537 Size: 275 378 1114 4090 Fragments arranged by size: 4090 1114 378 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2291 2937 3035 3787 1353 Size: 938 646 98 752 3423 Fragments arranged by size: 3423 938 752 646 98 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2362 4444 4818 4964 387 Size: 1064 138 773 2082 374 146 1280 Fragments arranged by size: 2082 1280 1064 773 374 146 138 EcoICRI GAG'CTC Cuts at: 2155 2155 Size: 5857 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2333 2345 2780 3920 4517 5304 5572 Size: 393 701 12 435 1140 597 787 268 Cuts at: 5572 1239 Size: 1524 Fragments arranged by size: 1524 1140 787 701 597 435 393 268 12 EcoO109I rG'GnC_Cy Cuts at: 2215 2235 2329 2215 Size: 20 94 5743 Fragments arranged by size: 5743 94 20 EcoRI G'AATT_C Cuts at: 3166 3544 3166 Size: 378 5479 FspI TGC'GCA Cuts at: 2277 4363 2277 Size: 2086 3771 HaeII r_GCGC'y Cuts at: 1678 2127 2232 2895 5004 1678 Size: 449 105 663 2109 2531 Fragments arranged by size: 2531 2109 663 449 105 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2144 2176 2847 2879 4498 Size: 32 135 32 572 32 671 32 1619 Cuts at: 4498 4530 4572 4604 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1619 671 572 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3201 3447 669 Size: 988 281 1263 246 3079 Fragments arranged by size: 3079 1263 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5857 HpaI GTT'AAC Cuts at: 3447 3447 Size: 5857 KasI G'GCGC_C Cuts at: 2228 2228 Size: 5857 KpnI G_GTAC'C Cuts at: 3182 3182 Size: 5857 MfeI C'AATT_G Cuts at: 3456 3456 Size: 5857 MluI A'CGCG_T Cuts at: 5733 5733 Size: 5857 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4971 5155 1305 Size: 3666 184 2007 Fragments arranged by size: 3666 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4035 Fragments arranged by size: 4035 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3815 4174 4333 518 Size: 648 2649 359 159 2042 Fragments arranged by size: 2649 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 2241 2346 2443 2986 3122 3273 3950 Size: 408 227 105 97 543 136 151 677 Cuts at: 3950 5098 5343 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 543 408 245 227 151 136 105 97 NaeI GCC'GGC Cuts at: 2194 2227 2194 Size: 33 5824 NarI GG'CG_CC Cuts at: 2229 2229 Size: 5857 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5305 NdeI CA'TA_TG Cuts at: 387 387 Size: 5857 NgoMIV G'CCGG_C Cuts at: 2192 2225 2192 Size: 33 5824 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5857 NotI GC'GGCC_GC Cuts at: 3304 3304 Size: 5857 NruI TCG'CGA Cuts at: 2938 2938 Size: 5857 NsiI A_TGCA'T Cuts at: 2394 3097 2394 Size: 703 5154 NspI r_CATG'y Cuts at: 1132 1229 2353 2995 3215 4760 1132 Size: 97 1124 642 220 1545 2229 Fragments arranged by size: 2229 1545 1124 642 220 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5857 PciI A'CATG_T Cuts at: 4756 4756 Size: 5857 PflMI CCAn_nnn'nTGG Cuts at: 1108 2911 1108 Size: 1803 4054 PfoI T'CCnGG_A Cuts at: 2082 2289 2082 Size: 207 5650 PmeI GTTT'AAAC Cuts at: 3161 3161 Size: 5857 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3926 3958 5439 5471 3926 Size: 32 1481 32 4312 Fragments arranged by size: 4312 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2215 2215 Size: 5857 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5857 PsiI TTA'TAA Cuts at: 3427 3427 Size: 5857 PspOMI G'GGCC_C Cuts at: 2235 2235 Size: 5857 PstI C_TGCA'G Cuts at: 830 2568 2742 3209 830 Size: 1738 174 467 3478 Fragments arranged by size: 3478 1738 467 174 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5857 PvuII CAG'CTG Cuts at: 2014 2986 2014 Size: 972 4885 SacI G_AGCT'C Cuts at: 2157 2157 Size: 5857 SacII CC_GC'GG Cuts at: 2242 2242 Size: 5857 SalI G'TCGA_C Cuts at: 1936 3199 1936 Size: 1263 4594 SanDI GG'GwC_CC Cuts at: 2215 2215 Size: 5857 SapI GCTCTTCn'nnn_ Cuts at: 2937 2937 Size: 5857 SbfI CC_TGCA'GG Cuts at: 3209 3209 Size: 5857 ScaI AGT'ACT Cuts at: 2957 4105 5817 2957 Size: 1148 1712 2997 Fragments arranged by size: 2997 1712 1148 SexAI A'CCwGG_T Cuts at: 2677 2677 Size: 5857 SfcI C'TryA_G Cuts at: 826 1045 2564 2738 3205 4340 5021 5212 826 Size: 219 1519 174 467 1135 681 191 1471 Fragments arranged by size: 1519 1471 1135 681 467 219 191 174 SfoI GGC'GCC Cuts at: 2230 2230 Size: 5857 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5857 SmaI CCC'GGG Cuts at: 1872 2211 2220 3184 1872 Size: 339 9 964 4545 Fragments arranged by size: 4545 964 339 9 SmlI C'TyrA_G Cuts at: 820 1017 1943 2156 2882 3961 4862 5124 820 Size: 197 926 213 726 1079 901 262 1553 Fragments arranged by size: 1553 1079 926 901 726 262 213 197 SnaBI TAC'GTA Cuts at: 493 3648 493 Size: 3155 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5857 SphI G_CATG'C Cuts at: 1132 3215 1132 Size: 2083 3774 SspI AAT'ATT Cuts at: 5 52 3781 5 Size: 47 3729 2081 Fragments arranged by size: 3729 2081 47 StuI AGG'CCT Cuts at: 1517 2560 1517 Size: 1043 4814 StyI C'CwwG_G Cuts at: 513 1065 2778 2859 3296 513 Size: 552 1713 81 437 3074 Fragments arranged by size: 3074 1713 552 437 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2660 2664 2700 3884 3901 4054 4239 Size: 1255 1080 4 36 1184 17 153 185 Cuts at: 4239 4684 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1184 1080 445 185 153 36 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2352 2838 2955 Size: 274 80 33 51 1443 375 486 117 Cuts at: 2955 4103 5815 96 Size: 1148 1712 138 Fragments arranged by size: 1712 1443 1148 486 375 274 138 117 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3053 3085 5817 5849 3053 Size: 32 2732 32 3061 Fragments arranged by size: 3061 2732 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5857 XbaI T'CTAG_A Cuts at: 3193 3193 Size: 5857 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5857 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5857 XmaI C'CCGG_G Cuts at: 1870 2209 2218 3182 1870 Size: 339 9 964 4545 Fragments arranged by size: 4545 964 339 9 XmnI GAAnn'nnTTC Cuts at: 3986 3986 Size: 5857 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3665 276 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FalI FseI FspAI PacI PmlI PspXI PsrI RsrII SfiI SrfI SwaI