(Linear) (Six-base) MAPSORT of: KIBB5872.seq Check: 8222 from: 1 to: 1152 KIBB5872.orf With 182 enzymes: SgfI * July 3, 2012 16:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 531 905 1152 Size: 531 374 247 Fragments arranged by size: 531 374 247 AccI GT'mk_AC Cuts at: 0 362 1152 Size: 362 790 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 775 1152 Size: 340 435 377 Fragments arranged by size: 435 377 340 AfeI AGC'GCT Cuts at: 0 888 1152 Size: 888 264 AflII C'TTAA_G Cuts at: 0 877 1152 Size: 877 275 AlwNI CAG_nnn'CTG Cuts at: 0 732 906 1152 Size: 732 174 246 Fragments arranged by size: 732 246 174 ApaI G_GGCC'C Cuts at: 0 234 1152 Size: 234 918 ApaLI G'TGCA_C Cuts at: 0 333 870 1152 Size: 333 537 282 Fragments arranged by size: 537 333 282 AscI GG'CGCG_CC Cuts at: 0 53 1152 Size: 53 1099 AvaI C'yCGr_G Cuts at: 0 130 204 213 253 402 1152 Size: 130 74 9 40 149 750 Fragments arranged by size: 750 149 130 74 40 9 BamHI G'GATC_C Cuts at: 0 200 1152 Size: 200 952 BanI G'GyrC_C Cuts at: 0 223 1152 Size: 223 929 BanII G_rGCy'C Cuts at: 0 152 234 305 1152 Size: 152 82 71 847 Fragments arranged by size: 847 152 82 71 BbeI G_GCGC'C Cuts at: 0 227 1152 Size: 227 925 BbsI GAAGACnn'nnnn_ Cuts at: 0 763 1152 Size: 763 389 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1128 1152 Size: 1128 24 BfrBI ATG'CAT Cuts at: 0 387 1090 1152 Size: 387 703 62 Fragments arranged by size: 703 387 62 Bme1580I G_kGCm'C Cuts at: 0 234 337 713 874 1152 Size: 234 103 376 161 278 Fragments arranged by size: 376 278 234 161 103 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 328 1152 Size: 328 824 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 136 1152 Size: 136 1016 BsaI GGTCTCn'nnnn_ Cuts at: 0 349 1152 Size: 349 803 BsaHI Gr'CG_yC Cuts at: 0 224 1152 Size: 224 928 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 106 1152 Size: 106 1046 BseYI C'CCAG_C Cuts at: 0 422 681 1122 1152 Size: 422 259 441 30 Fragments arranged by size: 441 422 259 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 424 503 967 1152 Size: 424 79 464 185 Fragments arranged by size: 464 424 185 79 BsiEI CG_ry'CG Cuts at: 0 188 747 1045 1152 Size: 188 559 298 107 Fragments arranged by size: 559 298 188 107 BsiHKAI G_wGCw'C Cuts at: 0 152 337 874 1151 1152 Size: 152 185 537 277 1 Fragments arranged by size: 537 277 185 152 1 BsmI GAATG_Cn' Cuts at: 0 1090 1152 Size: 1090 62 Bsp1286I G_dGCh'C Cuts at: 0 152 234 305 337 713 874 1151 1152 Size: 152 82 71 32 376 161 277 1 Fragments arranged by size: 376 277 161 152 82 71 32 1 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 531 905 1152 Size: 531 374 247 Fragments arranged by size: 531 374 247 BsrDI GCAATG_nn' Cuts at: 0 860 1152 Size: 860 292 BsrFI r'CCGG_y Cuts at: 0 124 187 220 1152 Size: 124 63 33 932 Fragments arranged by size: 932 124 63 33 BsrGI T'GTAC_A Cuts at: 0 347 833 1152 Size: 347 486 319 Fragments arranged by size: 486 347 319 BssHII G'CGCG_C Cuts at: 0 53 1152 Size: 53 1099 BssSI C'ACGA_G Cuts at: 0 163 1152 Size: 163 989 BstAPI GCAn_nnn'nTGC Cuts at: 0 732 1152 Size: 732 420 BstEII G'GTnAC_C Cuts at: 0 676 1152 Size: 676 476 BstYI r'GATC_y Cuts at: 0 200 902 1152 Size: 200 702 250 Fragments arranged by size: 702 250 200 Bsu36I CC'TnA_GG Cuts at: 0 597 717 1152 Size: 597 120 435 Fragments arranged by size: 597 435 120 BtgI C'CryG_G Cuts at: 0 234 462 1152 Size: 234 228 690 Fragments arranged by size: 690 234 228 DraIII CAC_nnn'GTG Cuts at: 0 627 1152 Size: 627 525 EaeI y'GGCC_r Cuts at: 0 143 185 701 1152 Size: 143 42 516 451 Fragments arranged by size: 516 451 143 42 EagI C'GGCC_G Cuts at: 0 185 1152 Size: 185 967 EarI CTCTTCn'nnn_ Cuts at: 0 286 932 1030 1152 Size: 286 646 98 122 Fragments arranged by size: 646 286 122 98 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 357 1152 Size: 357 795 EcoICRI GAG'CTC Cuts at: 0 150 1152 Size: 150 1002 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 328 340 775 1152 Size: 328 12 435 377 Fragments arranged by size: 435 377 328 12 EcoO109I rG'GnC_Cy Cuts at: 0 210 230 324 1152 Size: 210 20 94 828 Fragments arranged by size: 828 210 94 20 FspI TGC'GCA Cuts at: 0 272 1152 Size: 272 880 HaeII r_GCGC'y Cuts at: 0 122 227 890 1152 Size: 122 105 663 262 Fragments arranged by size: 663 262 122 105 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 139 171 842 874 1152 Size: 139 32 671 32 278 Fragments arranged by size: 671 278 139 32 32 KasI G'GCGC_C Cuts at: 0 223 1152 Size: 223 929 MspA1I CmG'CkG Cuts at: 0 9 236 341 438 981 1117 1152 Size: 9 227 105 97 543 136 35 Fragments arranged by size: 543 227 136 105 97 35 9 NaeI GCC'GGC Cuts at: 0 189 222 1152 Size: 189 33 930 Fragments arranged by size: 930 189 33 NarI GG'CG_CC Cuts at: 0 224 1152 Size: 224 928 NgoMIV G'CCGG_C Cuts at: 0 187 220 1152 Size: 187 33 932 Fragments arranged by size: 932 187 33 NruI TCG'CGA Cuts at: 0 933 1152 Size: 933 219 NsiI A_TGCA'T Cuts at: 0 389 1092 1152 Size: 389 703 60 Fragments arranged by size: 703 389 60 NspI r_CATG'y Cuts at: 0 348 990 1152 Size: 348 642 162 Fragments arranged by size: 642 348 162 PflMI CCAn_nnn'nTGG Cuts at: 0 906 1152 Size: 906 246 PfoI T'CCnGG_A Cuts at: 0 77 284 1152 Size: 77 207 868 Fragments arranged by size: 868 207 77 PpuMI rG'GwC_Cy Cuts at: 0 210 1152 Size: 210 942 PspOMI G'GGCC_C Cuts at: 0 230 1152 Size: 230 922 PstI C_TGCA'G Cuts at: 0 563 737 1152 Size: 563 174 415 Fragments arranged by size: 563 415 174 PvuII CAG'CTG Cuts at: 0 9 981 1152 Size: 9 972 171 Fragments arranged by size: 972 171 9 SacI G_AGCT'C Cuts at: 0 152 1152 Size: 152 1000 SacII CC_GC'GG Cuts at: 0 237 1152 Size: 237 915 SanDI GG'GwC_CC Cuts at: 0 210 1152 Size: 210 942 SapI GCTCTTCn'nnn_ Cuts at: 0 932 1152 Size: 932 220 ScaI AGT'ACT Cuts at: 0 952 1152 Size: 952 200 SexAI A'CCwGG_T Cuts at: 0 672 1152 Size: 672 480 SfcI C'TryA_G Cuts at: 0 559 733 1152 Size: 559 174 419 Fragments arranged by size: 559 419 174 SfoI GGC'GCC Cuts at: 0 225 1152 Size: 225 927 SmaI CCC'GGG Cuts at: 0 206 215 1152 Size: 206 9 937 Fragments arranged by size: 937 206 9 SmlI C'TyrA_G Cuts at: 0 151 877 1152 Size: 151 726 275 Fragments arranged by size: 726 275 151 StuI AGG'CCT Cuts at: 0 555 1152 Size: 555 597 StyI C'CwwG_G Cuts at: 0 773 854 1152 Size: 773 81 298 Fragments arranged by size: 773 298 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 655 659 695 1152 Size: 655 4 36 457 Fragments arranged by size: 655 457 36 4 TatI w'GTAC_w Cuts at: 0 347 833 950 1152 Size: 347 486 117 202 Fragments arranged by size: 486 347 202 117 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1048 1080 1152 Size: 1048 32 72 Fragments arranged by size: 1048 72 32 XmaI C'CCGG_G Cuts at: 0 204 213 1152 Size: 204 9 939 Fragments arranged by size: 939 204 9 Enzymes that do cut: AarI AccI AcuI AfeI AflII AlwNI ApaI ApaLI AscI AvaI BamHI BanI BanII BbeI BbsI BcgI BfrBI Bme1580I BpmI BpuEI BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstYI Bsu36I BtgI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I KasI MspA1I NaeI NarI NgoMIV NruI NsiI NspI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI ScaI SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI TstI XmaI Enzymes that do not cut: AatII Acc65I AclI AflIII AgeI AhdI AleI AlfI AloI ApoI AseI AsiSI AvrII BaeI BbvCI BciVI BclI BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsaXI BsiWI BsmBI BspEI BspHI BsrBI BstBI BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI DrdI EcoNI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI NcoI NdeI NheI NotI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SbfI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI