(Circular) (Six-base) MAPSORT of: pFN21AE9537.seq Check: 1343 from: 1 to: 5677 pFN21AE9537 5677 bp With 182 enzymes: SgfI * December 28, 2011 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3487 278 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3020 1937 Size: 1083 4594 Acc65I G'GTAC_C Cuts at: 2998 2998 Size: 5677 AclI AA'CG_TT Cuts at: 1563 3804 4177 1563 Size: 2241 373 3063 Fragments arranged by size: 3063 2241 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2237 2682 2784 3740 5124 5392 1632 Size: 605 445 102 956 1384 268 1917 Fragments arranged by size: 1917 1384 956 605 445 268 102 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5480 AflIII A'CryG_T Cuts at: 1203 2053 4576 5553 1203 Size: 850 2523 977 1327 Fragments arranged by size: 2523 1327 977 850 AgeI A'CCGG_T Cuts at: 2974 4508 2974 Size: 1534 4143 AhdI GACnn_n'nnGTC Cuts at: 4406 4406 Size: 5677 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5677 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5677 AlwNI CAG_nnn'CTG Cuts at: 2021 2453 2705 2816 3420 4992 2021 Size: 432 252 111 604 1572 2706 Fragments arranged by size: 2706 1572 604 432 252 111 ApaI G_GGCC'C Cuts at: 2508 2508 Size: 5677 ApaLI G'TGCA_C Cuts at: 3734 4890 3734 Size: 1156 4521 ApoI r'AATT_y Cuts at: 1493 2857 2986 3212 3364 5473 1493 Size: 1364 129 226 152 2109 1697 Fragments arranged by size: 2109 1697 1364 226 152 129 AseI AT'TA_AT Cuts at: 160 1032 4231 160 Size: 872 3199 1606 Fragments arranged by size: 3199 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 AvaI C'yCGr_G Cuts at: 1870 1943 2241 3002 1870 Size: 73 298 761 4545 Fragments arranged by size: 4545 761 298 73 BamHI G'GATC_C Cuts at: 3007 3374 3007 Size: 367 5310 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2090 2998 4453 618 Size: 325 217 627 303 908 1455 1842 Fragments arranged by size: 1842 1455 908 627 325 303 217 BanII G_rGCy'C Cuts at: 1391 2469 2508 2996 1391 Size: 1078 39 488 4072 Fragments arranged by size: 4072 1078 488 39 BbeI G_GCGC'C Cuts at: 2094 2094 Size: 5677 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2077 928 Size: 971 178 4528 Fragments arranged by size: 4528 971 178 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3868 3902 1903 Size: 34 1931 34 3678 Fragments arranged by size: 3678 1931 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3569 4779 3569 Size: 1210 4467 BclI T'GATC_A Cuts at: 1548 1548 Size: 5677 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4288 136 Size: 107 122 71 631 605 2616 1525 Fragments arranged by size: 2616 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5672 5672 Size: 5677 BlpI GC'TnA_GC Cuts at: 2025 3094 2025 Size: 1069 4608 Bme1580I G_kGCm'C Cuts at: 1790 2508 3738 4894 1790 Size: 718 1230 1156 2573 Fragments arranged by size: 2573 1230 1156 718 BmgBI CAC'GTC Cuts at: 1318 2056 1318 Size: 738 4939 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2329 2431 3408 Size: 416 53 83 399 24 912 102 977 Cuts at: 3408 4366 442 Size: 958 1753 Fragments arranged by size: 1753 977 958 912 416 399 102 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5677 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2135 2135 Size: 5677 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4337 1239 Size: 3098 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2165 3802 4667 4965 2165 Size: 1637 865 298 2877 Fragments arranged by size: 2877 1637 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4340 882 Size: 617 2841 2219 Fragments arranged by size: 2841 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3468 493 Size: 711 2264 2702 Fragments arranged by size: 2702 2264 711 BsaBI GATnn'nnATC Cuts at: 5671 5671 Size: 5677 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2091 3484 3866 4501 Size: 53 83 186 936 558 1393 382 635 Cuts at: 4501 275 Size: 1451 Fragments arranged by size: 1451 1393 936 635 558 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2974 4110 4508 4782 4929 5340 2974 Size: 1136 398 274 147 411 3311 Fragments arranged by size: 3311 1136 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2241 2271 1178 Size: 30 165 30 838 30 4584 Fragments arranged by size: 4584 838 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5677 BseYI C'CCAG_C Cuts at: 4880 5376 4880 Size: 496 5181 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2283 2829 1163 Size: 573 547 546 4011 Fragments arranged by size: 4011 573 547 546 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2299 2712 3127 3888 4916 1540 Size: 275 186 298 413 415 761 1028 2301 Fragments arranged by size: 2301 1028 761 415 413 298 275 186 BsiHKAI G_wGCw'C Cuts at: 2181 3738 3823 4894 2181 Size: 1557 85 1071 2964 Fragments arranged by size: 2964 1557 1071 85 BsmI GAATG_Cn' Cuts at: 2578 3188 3281 2578 Size: 610 93 4974 Fragments arranged by size: 4974 610 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2469 2508 2996 3738 3823 4894 Size: 399 391 288 39 488 742 85 1071 Cuts at: 4894 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 488 399 391 288 85 39 BspEI T'CCGG_A Cuts at: 5340 5340 Size: 5677 BspHI T'CATG_A Cuts at: 3566 5296 3566 Size: 1730 3947 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3032 844 Size: 2188 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2238 3564 1394 Size: 429 415 1326 3507 Fragments arranged by size: 3507 1326 429 415 BsrDI GCAATG_nn' Cuts at: 65 1248 4172 4346 65 Size: 1183 2924 174 1396 Fragments arranged by size: 2924 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2974 4321 4508 1700 Size: 110 1164 1347 187 2869 Fragments arranged by size: 2869 1347 1164 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5677 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5677 BssSI C'ACGA_G Cuts at: 3737 4749 3737 Size: 1012 4665 BstAPI GCAn_nnn'nTGC Cuts at: 794 2021 794 Size: 1227 4450 BstBI TT'CG_AA Cuts at: 4481 4481 Size: 5677 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5677 BstYI r'GATC_y Cuts at: 1972 2610 3007 3374 3759 3776 5228 5314 5326 Size: 638 397 367 385 17 1452 86 12 Cuts at: 5326 5672 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 638 397 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2172 2205 513 Size: 552 1107 33 3985 Fragments arranged by size: 3985 1107 552 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4938 Fragments arranged by size: 4938 683 56 BtsI GCAGTG_nn' Cuts at: 3193 3987 4007 3193 Size: 794 20 4863 Fragments arranged by size: 4863 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5677 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5642 DraI TTT'AAA Cuts at: 2981 2981 Size: 5677 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2054 4684 809 Size: 507 738 2630 1802 Fragments arranged by size: 2630 1802 738 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2578 3124 4013 Size: 54 1425 50 275 18 748 546 889 Cuts at: 4013 5642 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 748 546 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3124 1537 Size: 275 1312 4090 Fragments arranged by size: 4090 1312 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2552 2595 3607 1353 Size: 1199 43 1012 3423 Fragments arranged by size: 3423 1199 1012 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4264 4638 4784 387 Size: 1064 138 2675 374 146 1280 Fragments arranged by size: 2675 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 2682 2784 3740 4337 5124 5392 Size: 393 605 445 102 956 597 787 268 Cuts at: 5392 1239 Size: 1524 Fragments arranged by size: 1524 956 787 605 597 445 393 268 102 EcoO109I rG'GnC_Cy Cuts at: 2192 2401 2504 2505 2790 2192 Size: 209 103 1 285 5079 Fragments arranged by size: 5079 285 209 103 1 EcoRI G'AATT_C Cuts at: 2857 2986 3364 2857 Size: 129 378 5170 Fragments arranged by size: 5170 378 129 FspI TGC'GCA Cuts at: 4183 4183 Size: 5677 HaeII r_GCGC'y Cuts at: 1678 2094 4824 1678 Size: 416 2730 2531 Fragments arranged by size: 2730 2531 416 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2292 2324 2816 2848 4318 Size: 32 135 32 720 32 492 32 1470 Cuts at: 4318 4350 4392 4424 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1470 720 492 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3021 3267 669 Size: 988 281 1083 246 3079 Fragments arranged by size: 3079 1083 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5677 HpaI GTT'AAC Cuts at: 3267 3267 Size: 5677 KasI G'GCGC_C Cuts at: 2090 2090 Size: 5677 KpnI G_GTAC'C Cuts at: 3002 3002 Size: 5677 MfeI C'AATT_G Cuts at: 3276 3276 Size: 5677 MluI A'CGCG_T Cuts at: 5553 5553 Size: 5677 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2442 4791 4975 1305 Size: 1137 2349 184 2007 Fragments arranged by size: 2349 2007 1137 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2580 10 Size: 54 1425 343 748 3107 Fragments arranged by size: 3107 1425 748 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3635 3994 4153 518 Size: 648 2469 359 159 2042 Fragments arranged by size: 2469 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2018 3093 3770 4918 5163 1606 Size: 412 1075 677 1148 245 2120 Fragments arranged by size: 2120 1148 1075 677 412 245 NarI GG'CG_CC Cuts at: 2091 2091 Size: 5677 NcoI C'CATG_G Cuts at: 513 1065 2172 513 Size: 552 1107 4018 Fragments arranged by size: 4018 1107 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5677 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5677 NotI GC'GGCC_GC Cuts at: 3124 3124 Size: 5677 NspI r_CATG'y Cuts at: 1132 1229 2145 2202 2377 3035 4580 1132 Size: 97 916 57 175 658 1545 2229 Fragments arranged by size: 2229 1545 916 658 175 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5677 PciI A'CATG_T Cuts at: 4576 4576 Size: 5677 PflMI CCAn_nnn'nTGG Cuts at: 1108 2620 1108 Size: 1512 4165 PfoI T'CCnGG_A Cuts at: 2722 2722 Size: 5677 PmeI GTTT'AAAC Cuts at: 2981 2981 Size: 5677 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3746 3778 5259 5291 3746 Size: 32 1481 32 4132 Fragments arranged by size: 4132 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2401 2790 2401 Size: 389 5288 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5677 PsiI TTA'TAA Cuts at: 3247 3247 Size: 5677 PspOMI G'GGCC_C Cuts at: 2504 2504 Size: 5677 PstI C_TGCA'G Cuts at: 830 2017 2274 2496 2938 3029 830 Size: 1187 257 222 442 91 3478 Fragments arranged by size: 3478 1187 442 257 222 91 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5677 PvuII CAG'CTG Cuts at: 2018 2018 Size: 5677 SalI G'TCGA_C Cuts at: 1936 3019 1936 Size: 1083 4594 SanDI GG'GwC_CC Cuts at: 2401 2401 Size: 5677 SbfI CC_TGCA'GG Cuts at: 3029 3029 Size: 5677 ScaI AGT'ACT Cuts at: 3925 5637 3925 Size: 1712 3965 SfcI C'TryA_G Cuts at: 826 1045 2013 2270 2492 2640 2934 3025 4160 Size: 219 968 257 222 148 294 91 1135 Cuts at: 4160 4841 5032 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 968 681 294 257 222 219 191 148 91 SfoI GGC'GCC Cuts at: 2092 2092 Size: 5677 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5677 SmaI CCC'GGG Cuts at: 1872 2243 3004 1872 Size: 371 761 4545 Fragments arranged by size: 4545 761 371 SmlI C'TyrA_G Cuts at: 820 1017 1943 2180 3781 4682 4944 820 Size: 197 926 237 1601 901 262 1553 Fragments arranged by size: 1601 1553 926 901 262 237 197 SnaBI TAC'GTA Cuts at: 493 3468 493 Size: 2975 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5677 SphI G_CATG'C Cuts at: 1132 2145 2377 3035 1132 Size: 1013 232 658 3774 Fragments arranged by size: 3774 1013 658 232 SspI AAT'ATT Cuts at: 5 52 2661 3601 5 Size: 47 2609 940 2081 Fragments arranged by size: 2609 2081 940 47 StuI AGG'CCT Cuts at: 1517 2873 1517 Size: 1356 4321 StyI C'CwwG_G Cuts at: 513 1065 2172 3116 513 Size: 552 1107 944 3074 Fragments arranged by size: 3074 1107 944 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2313 3704 3721 3874 4059 4504 325 Size: 1255 733 1391 17 153 185 445 1498 Fragments arranged by size: 1498 1391 1255 733 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3923 5635 96 Size: 274 80 33 51 1443 1946 1712 138 Fragments arranged by size: 1946 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5637 5669 5637 Size: 32 5645 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5677 XbaI T'CTAG_A Cuts at: 3013 3013 Size: 5677 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5677 XmaI C'CCGG_G Cuts at: 1870 2241 3002 1870 Size: 371 761 4545 Fragments arranged by size: 4545 761 371 XmnI GAAnn'nnTTC Cuts at: 3806 3806 Size: 5677 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3485 276 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI