Restriction map for pFN21AE9537
 (Circular) (Six-base) MAPSORT of: pFN21AE9537.seq  Check: 1343  from: 1  to: 5677



pFN21AE9537  5677 bp

 With 182 enzymes: SgfI * 

                        December 28, 2011 15:23  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3487     278 
   Size:         53      83     186     936    1951    2468

  Fragments arranged by size: 

                2468    1951     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3020    1937 
   Size:       1083    4594
 
Acc65I G'GTAC_C

Cuts at:   2998    2998 
   Size:       5677
 
AclI AA'CG_TT

Cuts at:   1563    3804    4177    1563 
   Size:       2241     373    3063

  Fragments arranged by size: 

                3063    2241     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2237    2682    2784    3740    5124    5392    1632 
   Size:        605     445     102     956    1384     268    1917

  Fragments arranged by size: 

                1917    1384     956     605     445     268     102
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5480
 
AflIII A'CryG_T

Cuts at:   1203    2053    4576    5553    1203 
   Size:        850    2523     977    1327

  Fragments arranged by size: 

                2523    1327     977     850
 
AgeI A'CCGG_T

Cuts at:   2974    4508    2974 
   Size:       1534    4143
 
AhdI GACnn_n'nnGTC

Cuts at:   4406    4406 
   Size:       5677
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5677
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5677
 
AlwNI CAG_nnn'CTG

Cuts at:   2021    2453    2705    2816    3420    4992    2021 
   Size:        432     252     111     604    1572    2706

  Fragments arranged by size: 

                2706    1572     604     432     252     111
 
ApaI G_GGCC'C

Cuts at:   2508    2508 
   Size:       5677
 
ApaLI G'TGCA_C

Cuts at:   3734    4890    3734 
   Size:       1156    4521
 
ApoI r'AATT_y

Cuts at:   1493    2857    2986    3212    3364    5473    1493 
   Size:       1364     129     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1364     226     152     129
 
AseI AT'TA_AT

Cuts at:    160    1032    4231     160 
   Size:        872    3199    1606

  Fragments arranged by size: 

                3199    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5677
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2241    3002    1870 
   Size:         73     298     761    4545

  Fragments arranged by size: 

                4545     761     298      73
 
BamHI G'GATC_C

Cuts at:   3007    3374    3007 
   Size:        367    5310
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2090    2998    4453     618 
   Size:        325     217     627     303     908    1455    1842

  Fragments arranged by size: 

                1842    1455     908     627     325     303     217
 
BanII G_rGCy'C

Cuts at:   1391    2469    2508    2996    1391 
   Size:       1078      39     488    4072

  Fragments arranged by size: 

                4072    1078     488      39
 
BbeI G_GCGC'C

Cuts at:   2094    2094 
   Size:       5677
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2077     928 
   Size:        971     178    4528

  Fragments arranged by size: 

                4528     971     178
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3868    3902    1903 
   Size:         34    1931      34    3678

  Fragments arranged by size: 

                3678    1931      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3569    4779    3569 
   Size:       1210    4467
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5677
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4288     136 
   Size:        107     122      71     631     605    2616    1525

  Fragments arranged by size: 

                2616    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5672    5672 
   Size:       5677
 
BlpI GC'TnA_GC

Cuts at:   2025    3094    2025 
   Size:       1069    4608
 
Bme1580I G_kGCm'C

Cuts at:   1790    2508    3738    4894    1790 
   Size:        718    1230    1156    2573

  Fragments arranged by size: 

                2573    1230    1156     718
 
BmgBI CAC'GTC

Cuts at:   1318    2056    1318 
   Size:        738    4939
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2329    2431    3408 
   Size:        416      53      83     399      24     912     102     977

Cuts at:   3408    4366     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753     977     958     912     416     399     102      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5677
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2135    2135 
   Size:       5677
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4337    1239 
   Size:       3098    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2165    3802    4667    4965    2165 
   Size:       1637     865     298    2877

  Fragments arranged by size: 

                2877    1637     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4340     882 
   Size:        617    2841    2219

  Fragments arranged by size: 

                2841    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3468     493 
   Size:        711    2264    2702

  Fragments arranged by size: 

                2702    2264     711
 
BsaBI GATnn'nnATC

Cuts at:   5671    5671 
   Size:       5677
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2091    3484    3866    4501 
   Size:         53      83     186     936     558    1393     382     635

Cuts at:   4501     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1393     936     635     558     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2974    4110    4508    4782    4929    5340    2974 
   Size:       1136     398     274     147     411    3311

  Fragments arranged by size: 

                3311    1136     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2241    2271    1178 
   Size:         30     165      30     838      30    4584

  Fragments arranged by size: 

                4584     838     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5677
 
BseYI C'CCAG_C

Cuts at:   4880    5376    4880 
   Size:        496    5181
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2283    2829    1163 
   Size:        573     547     546    4011

  Fragments arranged by size: 

                4011     573     547     546
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2299    2712    3127    3888    4916    1540 
   Size:        275     186     298     413     415     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     415     413     298     275     186
            
 
BsiHKAI G_wGCw'C

Cuts at:   2181    3738    3823    4894    2181 
   Size:       1557      85    1071    2964

  Fragments arranged by size: 

                2964    1557    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2578    3188    3281    2578 
   Size:        610      93    4974

  Fragments arranged by size: 

                4974     610      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2181    2469    2508    2996    3738    3823    4894 
   Size:        399     391     288      39     488     742      85    1071

Cuts at:   4894    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     488     399     391     288      85
                  39
 
BspEI T'CCGG_A

Cuts at:   5340    5340 
   Size:       5677
 
BspHI T'CATG_A

Cuts at:   3566    5296    3566 
   Size:       1730    3947
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3032     844 
   Size:       2188    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2238    3564    1394 
   Size:        429     415    1326    3507

  Fragments arranged by size: 

                3507    1326     429     415
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4172    4346      65 
   Size:       1183    2924     174    1396

  Fragments arranged by size: 

                2924    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2974    4321    4508    1700 
   Size:        110    1164    1347     187    2869

  Fragments arranged by size: 

                2869    1347    1164     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5677
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5677
 
BssSI C'ACGA_G

Cuts at:   3737    4749    3737 
   Size:       1012    4665
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2021     794 
   Size:       1227    4450
 
BstBI TT'CG_AA

Cuts at:   4481    4481 
   Size:       5677
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5677
 
BstYI r'GATC_y

Cuts at:   1972    2610    3007    3374    3759    3776    5228    5314    5326 
   Size:        638     397     367     385      17    1452      86      12

Cuts at:   5326    5672    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     638     397     385     367     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2172    2205     513 
   Size:        552    1107      33    3985

  Fragments arranged by size: 

                3985    1107     552      33
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4938

  Fragments arranged by size: 

                4938     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3193    3987    4007    3193 
   Size:        794      20    4863

  Fragments arranged by size: 

                4863     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5677
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5642
 
DraI TTT'AAA

Cuts at:   2981    2981 
   Size:       5677
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2054    4684     809 
   Size:        507     738    2630    1802

  Fragments arranged by size: 

                2630    1802     738     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2578    3124    4013 
   Size:         54    1425      50     275      18     748     546     889

Cuts at:   4013    5642       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     748     546     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3124    1537 
   Size:        275    1312    4090

  Fragments arranged by size: 

                4090    1312     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2552    2595    3607    1353 
   Size:       1199      43    1012    3423

  Fragments arranged by size: 

                3423    1199    1012      43
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4264    4638    4784     387 
   Size:       1064     138    2675     374     146    1280

  Fragments arranged by size: 

                2675    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2237    2682    2784    3740    4337    5124    5392 
   Size:        393     605     445     102     956     597     787     268

Cuts at:   5392    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     956     787     605     597     445     393     268
                 102
 
EcoO109I rG'GnC_Cy

Cuts at:   2192    2401    2504    2505    2790    2192 
   Size:        209     103       1     285    5079

  Fragments arranged by size: 

                5079     285     209     103       1
 
EcoRI G'AATT_C

Cuts at:   2857    2986    3364    2857 
   Size:        129     378    5170

  Fragments arranged by size: 

                5170     378     129
 
FspI TGC'GCA

Cuts at:   4183    4183 
   Size:       5677
 
HaeII r_GCGC'y

Cuts at:   1678    2094    4824    1678 
   Size:        416    2730    2531

  Fragments arranged by size: 

                2730    2531     416
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2292    2324    2816    2848    4318 
   Size:         32     135      32     720      32     492      32    1470

Cuts at:   4318    4350    4392    4424    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1470     720     492     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3021    3267     669 
   Size:        988     281    1083     246    3079

  Fragments arranged by size: 

                3079    1083     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5677
 
HpaI GTT'AAC

Cuts at:   3267    3267 
   Size:       5677
 
KasI G'GCGC_C

Cuts at:   2090    2090 
   Size:       5677
 
KpnI G_GTAC'C

Cuts at:   3002    3002 
   Size:       5677
 
MfeI C'AATT_G

Cuts at:   3276    3276 
   Size:       5677
 
MluI A'CGCG_T

Cuts at:   5553    5553 
   Size:       5677
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2442    4791    4975    1305 
   Size:       1137    2349     184    2007

  Fragments arranged by size: 

                2349    2007    1137     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2580      10 
   Size:         54    1425     343     748    3107

  Fragments arranged by size: 

                3107    1425     748     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3635    3994    4153     518 
   Size:        648    2469     359     159    2042

  Fragments arranged by size: 

                2469    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2018    3093    3770    4918    5163    1606 
   Size:        412    1075     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1075     677     412     245
 
NarI GG'CG_CC

Cuts at:   2091    2091 
   Size:       5677
 
NcoI C'CATG_G

Cuts at:    513    1065    2172     513 
   Size:        552    1107    4018

  Fragments arranged by size: 

                4018    1107     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5677
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5677
 
NotI GC'GGCC_GC

Cuts at:   3124    3124 
   Size:       5677
 
NspI r_CATG'y

Cuts at:   1132    1229    2145    2202    2377    3035    4580    1132 
   Size:         97     916      57     175     658    1545    2229

  Fragments arranged by size: 

                2229    1545     916     658     175      97      57
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5677
 
PciI A'CATG_T

Cuts at:   4576    4576 
   Size:       5677
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2620    1108 
   Size:       1512    4165
 
PfoI T'CCnGG_A

Cuts at:   2722    2722 
   Size:       5677
 
PmeI GTTT'AAAC

Cuts at:   2981    2981 
   Size:       5677
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3746    3778    5259    5291    3746 
   Size:         32    1481      32    4132

  Fragments arranged by size: 

                4132    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2401    2790    2401 
   Size:        389    5288
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5677
 
PsiI TTA'TAA

Cuts at:   3247    3247 
   Size:       5677
 
PspOMI G'GGCC_C

Cuts at:   2504    2504 
   Size:       5677
 
PstI C_TGCA'G

Cuts at:    830    2017    2274    2496    2938    3029     830 
   Size:       1187     257     222     442      91    3478

  Fragments arranged by size: 

                3478    1187     442     257     222      91
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5677
 
PvuII CAG'CTG

Cuts at:   2018    2018 
   Size:       5677
 
SalI G'TCGA_C

Cuts at:   1936    3019    1936 
   Size:       1083    4594
 
SanDI GG'GwC_CC

Cuts at:   2401    2401 
   Size:       5677
 
SbfI CC_TGCA'GG

Cuts at:   3029    3029 
   Size:       5677
 
ScaI AGT'ACT

Cuts at:   3925    5637    3925 
   Size:       1712    3965
 
SfcI C'TryA_G

Cuts at:    826    1045    2013    2270    2492    2640    2934    3025    4160 
   Size:        219     968     257     222     148     294      91    1135

Cuts at:   4160    4841    5032     826 
   Size:        681     191    1471

  Fragments arranged by size: 

                1471    1135     968     681     294     257     222     219
                 191     148      91
 
SfoI GGC'GCC

Cuts at:   2092    2092 
   Size:       5677
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5677
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5677
 
SmaI CCC'GGG

Cuts at:   1872    2243    3004    1872 
   Size:        371     761    4545

  Fragments arranged by size: 

                4545     761     371
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2180    3781    4682    4944     820 
   Size:        197     926     237    1601     901     262    1553

  Fragments arranged by size: 

                1601    1553     926     901     262     237     197
 
SnaBI TAC'GTA

Cuts at:    493    3468     493 
   Size:       2975    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5677
 
SphI G_CATG'C

Cuts at:   1132    2145    2377    3035    1132 
   Size:       1013     232     658    3774

  Fragments arranged by size: 

                3774    1013     658     232
 
SspI AAT'ATT

Cuts at:      5      52    2661    3601       5 
   Size:         47    2609     940    2081

  Fragments arranged by size: 

                2609    2081     940      47
 
StuI AGG'CCT

Cuts at:   1517    2873    1517 
   Size:       1356    4321
 
StyI C'CwwG_G

Cuts at:    513    1065    2172    3116     513 
   Size:        552    1107     944    3074

  Fragments arranged by size: 

                3074    1107     944     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2313    3704    3721    3874    4059    4504     325 
   Size:       1255     733    1391      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1391    1255     733     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3923    5635      96 
   Size:        274      80      33      51    1443    1946    1712     138

  Fragments arranged by size: 

                1946    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5637    5669    5637 
   Size:         32    5645
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5677
 
XbaI T'CTAG_A

Cuts at:   3013    3013 
   Size:       5677
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5677
 
XmaI C'CCGG_G

Cuts at:   1870    2241    3002    1870 
   Size:        371     761    4545

  Fragments arranged by size: 

                4545     761     371
 
XmnI GAAnn'nnTTC

Cuts at:   3806    3806 
   Size:       5677
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3485     276 
   Size:         53      83     186     936    1951    2468

  Fragments arranged by size: 

                2468    1951     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI     PstI     PvuI    PvuII     SalI    SanDI     SbfI
     ScaI     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI    BbvCI    BfrBI
   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI
    EcoNI    EcoRV     FalI     FseI    FspAI     NaeI   NgoMIV     NruI
     NsiI     PacI     PmlI    PspXI     PsrI    RsrII     SacI    SacII
     SapI    SexAI     SfiI     SrfI     SwaI     XcmI
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