Restriction map for
pFN21AE2508
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BclI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmI BspDI
BspEI BspLU11I BspMI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT14I FbaI FseI FspI
HaeII HaeIII HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 270 GTCTAC GT^[AC][GT]AC
AlwI 489 GGATC GGATC(4/5)
AlwNI 1214 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 223 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 204 CTCGAG C^[CT]CG[GA]G
BanII 1237 GAGCCC G[GA]GC[CT]^C
BciVI 472 GTATCC GTATCC(6/5)
BcnI 1134 CCGGG CC^[GC]GG
BmyI 1237 GAGCCC G[AGT]GC[ACT]^C
BsaI 920 GAGACC GGTCTC(1/5)
BsaJI 611 CCCAGG C^C[ACGT][ACGT]GG
BsaWI 413 ACCGGA [AT]^CCGG[AT]
BsgI 1300 GTGCAG GTGCAG(16/14)
BsiYI 689 CCCAAGAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 689 CCCAAGAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 247 GGGAC GGGAC(10/14)
BsoBI 204 CTCGAG C^[CT]CG[GA]G
Bsp1286I 1237 GAGCCC G[AGT]GC[ACT]^C
BspHI 1044 TCATGA T^CATGA
BsrBI 694 GAGCGG CCGCTC(-3/-3)
DpnII 490 GATC ^GATC
DraII 247 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 247 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 199 GATATC GAT^ATC
FauI 602 CCCGC CCCGC(4/6)
HgaI 268 GCGTC GACGC(5/10)
HincII 939 GTTGAC GT[CT]^[GA]AC
HindII 939 GTTGAC GT[CT]^[GA]AC
HphI 286 GGTGA GGTGA(8/7)
MaeIII 1324 GTCAC ^GT[ACGT]AC
MboI 490 GATC ^GATC
NciI 1134 CCGGG CC^[GC]GG
NdeII 490 GATC ^GATC
PaeR7I 204 CTCGAG C^TCGAG
PpuMI 247 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 1044 TCATGA T^CATGA
Sau3AI 490 GATC ^GATC
SmlI 204 CTCGAG C^T[CT][GA]AG
Tsp45I 1324 GTCAC ^GT[GC]AC
XhoI 204 CTCGAG C^TCGAG
XmnI 223 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 32 enzymes) #
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AciI 603 CCGC CCGC(-3/-1)
696 GCGG
AseI 327 ATTAAT AT^TAAT
399 ATTAAT
AsnI 327 ATTAAT AT^TAAT
399 ATTAAT
AvaII 96 GGACC G^G[AT]CC
248 GGACC
BfaI 427 CTAG C^TAG
1330 CTAG
BsmAI 920 GAGAC GTCTC(1/5)
786 GTCTC
Bsp1407I 2 TGTACA T^GTACA
1250 TGTACA
BsrDI 737 GCAATG GCAATG(2/0)
986 GCAATG
BsrGI 2 TGTACA T^GTACA
1250 TGTACA
BstF5I 608 CATCC GGATG(2/0)
450 GGATG
BstNI 30 CCTGG CC^[AT]GG
612 CCAGG
Cac8I 843 GCAGGC GC[ACGT]^[ACGT]GC
1162 GCCTGC
Cfr13I 96 GGACC G^G[ACGT]CC
248 GGACC
EcoRII 30 CCTGG ^CC[AT]GG
612 CCAGG
EcoT22I 280 ATGCAT ATGCA^T
763 ATGCAT
FokI 608 CATCC GGATG(9/13)
450 GGATG
HapII 414 CCGG C^CGG
1134 CCGG
HpaII 414 CCGG C^CGG
1134 CCGG
MaeI 427 CTAG C^TAG
1330 CTAG
MaeII 729 ACGT A^CGT
873 ACGT
MspA1I 848 CAGCTG C[AC]G^C[GT]G
904 CAGCTG
MspI 414 CCGG C^CGG
1134 CCGG
MvaI 30 CCTGG CC^[AT]GG
612 CCAGG
NlaIV 96 GGACCC GG[ACGT]^[ACGT]CC
247 GGGACC
NsiI 280 ATGCAT ATGCA^T
763 ATGCAT
PleI 942 GACTC GAGTC(4/5)
1312 GACTC
PshBI 327 ATTAAT AT^TAAT
399 ATTAAT
PvuII 848 CAGCTG CAG^CTG
904 CAGCTG
Sau96I 96 GGACC G^G[ACGT]CC
248 GGACC
SfaNI 178 GATGC GCATC(5/9)
265 GATGC
SspBI 2 TGTACA T^GTACA
1250 TGTACA
TfiI 421 GAATC G^A[AT]TC
791 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AfaI AluI BbsI BbvI BpuAI
BsrI BssKI DdeI Fnu4HI HinfI
ItaI MboII MnlI MseI MslI
MwoI NlaIII RsaI ScrFI SfcI
SspI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I