################################## # NO CUT ENZYMES ( 194 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeIII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AlwNI 56 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG AspI 93 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 132 GGACC G^G[AT]CC BbvI 67 GCTGC GCAGC(8/12) BciVI 514 GTATCC GTATCC(6/5) BfaI 221 CTAG C^TAG BpmI 266 CTGGAG CTGGAG(16/14) BsaI 9 GGTCTC GGTCTC(1/5) BsmAI 10 GTCTC GTCTC(1/5) BsrI 193 ACTGG ACTGG(1/-1) BstF5I 424 GGATG GGATG(2/0) BstYI 233 GGATCT [GA]^GATC[CT] Cac8I 247 GCTCGC GC[ACGT]^[ACGT]GC Cfr13I 132 GGACC G^G[ACGT]CC DdeI 28 CTGAG C^T[ACGT]AG EcoRV 178 GATATC GAT^ATC FokI 424 GGATG GGATG(9/13) HinfI 138 GACTC G^A[ACGT]TC MaeI 221 CTAG C^TAG MaeII 37 ACGT A^CGT MflI 233 GGATCT [GA]^GATC[CT] MseI 190 TTAA T^TAA NspI 257 ACATGC [GA]CATG^[CT] PflFI 93 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 138 GACTC GAGTC(4/5) SapI 205 GAAGAGC GCTCTTC(1/4) Sau96I 132 GGACC G^G[ACGT]CC ScaI 385 AGTACT AGT^ACT SexAI 94 ACCAGGT A^CC[AT]GGT SfcI 62 CTGTAG C^T[GA][CT]AG SmlI 140 CTCAAG C^T[CT][GA]AG Tru9I 190 TTAA T^TAA TseI 67 GCTGC G^C[AT]GC Tsp509I 173 AATT ^AATT Tth111I 93 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 233 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 25 GTAC GT^AC 386 GTAC AluI 66 AGCT AG^CT 430 AGCT AlwI 355 GATCC GGATC(4/5) 233 GGATC BsaJI 83 CCAGGG C^C[ACGT][ACGT]GG 308 CCAGGG BsiYI 77 CCGTTTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 328 CCTTTGATGGG BslI 77 CCGTTTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 328 CCTTTGATGGG DpnII 234 GATC ^GATC 355 GATC EarI 205 GAAGAG CTCTTC(1/4) 442 GAAGAG Fnu4HI 4 GCGGC GC^[ACGT]GC 67 GCTGC ItaI 4 GCGGC GC^[ACGT]GC 67 GCTGC Ksp632I 205 GAAGAG CTCTTC(1/4) 442 GAAGAG MboI 234 GATC ^GATC 355 GATC MwoI 61 GCTGTAGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 343 GCATCAACGGC NdeII 234 GATC ^GATC 355 GATC NlaIV 87 GGTTCC GG[ACGT]^[ACGT]CC 453 GGTTCC RsaI 25 GTAC GT^AC 386 GTAC Sau3AI 234 GATC ^GATC 355 GATC SfaNI 397 GATGC GCATC(5/9) 343 GCATC TspRI 20 CAGTG CA[GC]TG[ACGT][ACGT]^ 56 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### AciI BssKI BstNI EcoRII MboII MnlI MvaI NlaIII ScrFI