################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BalI BanII BbeI BbrPI BbsI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII Cfr10I CpoI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT22I FseI HaeII HapII HpaI HpaII KasI KpnI KspI MaeI MaeII MluI MluNI MroI MscI MspI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcyI 383 GGCGTC G[GA]^CG[CT]C AocI 865 CCTGAGG CC^T[ACGT]AGG AspHI 936 GTGCTC G[AT]GC[AT]^C AviII 2 TGCGCA TGC^GCA BanI 106 GGCACC G^G[CT][GA]CC BbvCI 1528 GCTGAGG CCTCAGC(-5/-2) BclI 1089 TGATCA T^GATCA BpmI 18 CTGGAG CTGGAG(16/14) BsaHI 383 GGCGTC G[GA]^CG[CT]C BseRI 326 GAGGAG GAGGAG(10/8) BsgI 572 CTGCAC GTGCAG(16/14) BsiHKAI 936 GTGCTC G[AT]GC[AT]^C BsmAI 319 GTCTC GTCTC(1/5) BsmFI 1459 GGGAC GGGAC(10/14) BsmI 486 GAATGC GAATGC(1/-1) BspDI 1499 ATCGAT AT^CGAT BspMI 780 GCAGGT ACCTGC(4/8) Bsu36I 865 CCTGAGG CC^T[ACGT]AGG CfoI 3 GCGC GCG^C ClaI 1499 ATCGAT AT^CGAT DraI 741 TTTAAA TTT^AAA DraII 842 AGGACCT [GA]G^G[ACGT]CC[CT] DrdI 1613 GACCTGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 72 CGGCCA [CT]^GGCC[GA] Eco81I 865 CCTGAGG CC^T[ACGT]AGG EcoO109I 842 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 814 GATATC GAT^ATC FauI 60 GCGGG CCCGC(4/6) FbaI 1089 TGATCA T^GATCA FspI 2 TGCGCA TGC^GCA HgaI 384 GCGTC GACGC(5/10) HhaI 3 GCGC GCG^C Hin1I 383 GGCGTC G[GA]^CG[CT]C HinP1I 3 GCGC G^CGC HindIII 238 AAGCTT A^AGCTT MfeI 1288 CAATTG C^AATTG MspA1I 957 CAGCTG C[AC]G^C[GT]G MunI 1288 CAATTG C^AATTG PflMI 1609 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 842 AGGACCT [GA]G^G[AT]CC[CT] PstI 778 CTGCAG CTGCA^G PvuII 957 CAGCTG CAG^CTG SalI 1244 GTCGAC G^TCGAC SexAI 272 ACCAGGT A^CC[AT]GGT SspI 737 AATATT AAT^ATT SwaI 740 ATTTAAAT ATTT^AAAT TspRI 630 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 1609 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 1244 GTCGAC GT^[AC][GT]AC 1376 GTCTAC AciI 216 CCGC CCGC(-3/-1) 60 GCGG AvaII 438 GGTCC G^G[AT]CC 843 GGACC BbvI 571 GCTGC GCAGC(8/12) 777 GCTGC BmyI 936 GTGCTC G[AGT]GC[ACT]^C 1151 GGGCAC BsaBI 1087 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1478 GATCGGGATC BsaJI 1147 CCAAGG C^C[ACGT][ACGT]GG 1197 CCAAGG Bsp1286I 936 GTGCTC G[AGT]GC[ACT]^C 1151 GGGCAC BsrI 145 ACTGG ACTGG(1/-1) 1413 ACTGG Cac8I 604 GCAGGC GC[ACGT]^[ACGT]GC 1153 GCACGC EcoT14I 1147 CCAAGG C^C[AT][AT]GG 1197 CCAAGG Fnu4HI 571 GCTGC GC^[ACGT]GC 777 GCTGC HincII 1244 GTCGAC GT[CT]^[GA]AC 1597 GTTGAC HindII 1244 GTCGAC GT[CT]^[GA]AC 1597 GTTGAC ItaI 571 GCTGC GC^[ACGT]GC 777 GCTGC MamI 1087 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1478 GATCGGGATC NdeI 430 CATATG CA^TATG 772 CATATG SfcI 778 CTGCAG C^T[GA][CT]AG 1378 CTACAG SmlI 235 CTCAAG C^T[CT][GA]AG 1535 CTCAAG StyI 1147 CCAAGG C^C[AT][AT]GG 1197 CCAAGG TseI 571 GCTGC G^C[AT]GC 777 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AcsI AfaI AluI AlwI AlwNI ApoI BamHI BsiYI BslI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EarI EcoRII FokI HaeIII HinfI HphI Ksp632I MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I Tsp45I Tsp509I TthHB8I XhoII