(Circular) (Six-base) MAPSORT of: pFN21AE3763.seq Check: 8576 from: 1 to: 6382 pFN21AE3763 6382 bp With 182 enzymes: SgfI * March 28, 2012 12:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2776 2776 Size: 6382 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4192 278 Size: 53 83 186 936 2656 2468 Fragments arranged by size: 2656 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3250 3382 3725 1937 Size: 1313 132 343 4594 Fragments arranged by size: 4594 1313 343 132 Acc65I G'GTAC_C Cuts at: 3703 3703 Size: 6382 AclI AA'CG_TT Cuts at: 1563 4509 4882 1563 Size: 2946 373 3063 Fragments arranged by size: 3063 2946 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4445 5829 6097 1632 Size: 2813 1384 268 1917 Fragments arranged by size: 2813 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6185 AflIII A'CryG_T Cuts at: 1203 5281 6258 1203 Size: 4078 977 1327 Fragments arranged by size: 4078 1327 977 AgeI A'CCGG_T Cuts at: 5213 5213 Size: 6382 AhdI GACnn_n'nnGTC Cuts at: 5111 5111 Size: 6382 AleI CACnn'nnGTG Cuts at: 1166 3204 1166 Size: 2038 4344 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6382 AlwNI CAG_nnn'CTG Cuts at: 2022 3467 3533 4125 5697 2022 Size: 1445 66 592 1572 2707 Fragments arranged by size: 2707 1572 1445 592 66 ApaLI G'TGCA_C Cuts at: 4439 5595 4439 Size: 1156 5226 ApoI r'AATT_y Cuts at: 1493 2229 2540 3638 3669 3691 3917 4069 6178 Size: 736 311 1098 31 22 226 152 2109 Cuts at: 6178 1493 Size: 1697 Fragments arranged by size: 2109 1697 1098 736 311 226 152 31 22 AseI AT'TA_AT Cuts at: 160 1032 4936 160 Size: 872 3904 1606 Fragments arranged by size: 3904 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6382 AvaI C'yCGr_G Cuts at: 1870 1943 3707 1870 Size: 73 1764 4545 Fragments arranged by size: 4545 1764 73 BamHI G'GATC_C Cuts at: 2791 2875 2989 3712 4079 2791 Size: 84 114 723 367 5094 Fragments arranged by size: 5094 723 367 114 84 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2111 3703 5158 618 Size: 325 217 627 324 1592 1455 1842 Fragments arranged by size: 1842 1592 1455 627 325 324 217 BanII G_rGCy'C Cuts at: 1391 3701 1391 Size: 2310 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5411 BbvCI CC'TCA_GC Cuts at: 3534 3534 Size: 6382 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4573 4607 1903 Size: 34 50 34 2552 34 3678 Fragments arranged by size: 3678 2552 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4274 5484 4274 Size: 1210 5172 BclI T'GATC_A Cuts at: 1548 3094 1548 Size: 1546 4836 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4993 136 Size: 107 122 71 631 605 3321 1525 Fragments arranged by size: 3321 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6377 6377 Size: 6382 BlpI GC'TnA_GC Cuts at: 3799 3799 Size: 6382 Bme1580I G_kGCm'C Cuts at: 1790 3160 4443 5599 1790 Size: 1370 1283 1156 2573 Fragments arranged by size: 2573 1370 1283 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6382 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2160 4113 5071 Size: 416 53 83 399 24 743 1953 958 Cuts at: 5071 442 Size: 1753 Fragments arranged by size: 1953 1753 958 743 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6382 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2044 5042 1239 Size: 805 2998 2579 Fragments arranged by size: 2998 2579 805 Bpu10I CC'TnA_GC Cuts at: 3534 3534 Size: 6382 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2225 3525 4507 5372 5670 2225 Size: 1300 982 865 298 2937 Fragments arranged by size: 2937 1300 982 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5045 882 Size: 617 3546 2219 Fragments arranged by size: 3546 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4173 493 Size: 711 2969 2702 Fragments arranged by size: 2969 2702 711 BsaBI GATnn'nnATC Cuts at: 3096 3487 6376 3096 Size: 391 2889 3102 Fragments arranged by size: 3102 2889 391 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2389 4189 4571 5206 Size: 53 83 186 936 856 1800 382 635 Cuts at: 5206 275 Size: 1451 Fragments arranged by size: 1800 1451 936 856 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4815 5213 5487 5634 6045 4815 Size: 398 274 147 411 5152 Fragments arranged by size: 5152 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3457 3487 1178 Size: 30 165 30 2054 30 4073 Fragments arranged by size: 4073 2054 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2346 1186 Size: 1160 5222 BseYI C'CCAG_C Cuts at: 2101 5585 6081 2101 Size: 3484 496 2402 Fragments arranged by size: 3484 2402 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2562 1163 Size: 573 826 4983 Fragments arranged by size: 4983 826 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3832 4593 5621 1540 Size: 275 186 1831 761 1028 2301 Fragments arranged by size: 2301 1831 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2945 4443 4528 5599 2945 Size: 1498 85 1071 3728 Fragments arranged by size: 3728 1498 1071 85 BsmI GAATG_Cn' Cuts at: 2497 3893 3986 2497 Size: 1396 93 4893 Fragments arranged by size: 4893 1396 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2945 3160 3701 4443 4528 5599 1391 Size: 399 1155 215 541 742 85 1071 2174 Fragments arranged by size: 2174 1155 1071 742 541 399 215 85 BspEI T'CCGG_A Cuts at: 6045 6045 Size: 6382 BspHI T'CATG_A Cuts at: 4271 6001 4271 Size: 1730 4652 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2776 3737 844 Size: 1932 961 3489 Fragments arranged by size: 3489 1932 961 BsrBI CCG'CTC Cuts at: 1394 1823 4269 1394 Size: 429 2446 3507 Fragments arranged by size: 3507 2446 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4877 5051 65 Size: 1183 3629 174 1396 Fragments arranged by size: 3629 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5026 5213 1700 Size: 110 3216 187 2869 Fragments arranged by size: 3216 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6382 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6382 BssSI C'ACGA_G Cuts at: 4442 5454 4442 Size: 1012 5370 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6382 BstBI TT'CG_AA Cuts at: 5186 5186 Size: 6382 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6382 BstYI r'GATC_y Cuts at: 1972 2403 2724 2791 2875 2989 3712 4079 4464 Size: 431 321 67 84 114 723 367 385 Cuts at: 4464 4481 5933 6019 6031 6377 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 723 431 385 367 346 321 114 86 84 67 17 12 Bsu36I CC'TnA_GG Cuts at: 2871 2871 Size: 6382 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5830 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5643 Fragments arranged by size: 5643 683 56 BtsI GCAGTG_nn' Cuts at: 2641 3898 4692 4712 2641 Size: 1257 794 20 4311 Fragments arranged by size: 4311 1257 794 20 ClaI AT'CG_AT Cuts at: 1554 3505 1554 Size: 1951 4431 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6347 DraI TTT'AAA Cuts at: 2748 3686 2748 Size: 938 5444 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3624 5389 809 Size: 507 2308 1765 1802 Fragments arranged by size: 2308 1802 1765 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2077 3829 4718 Size: 54 1425 50 275 18 247 1752 889 Cuts at: 4718 6347 8 Size: 1629 43 Fragments arranged by size: 1752 1629 1425 889 275 247 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3829 1537 Size: 275 2017 4090 Fragments arranged by size: 4090 2017 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2795 2943 3598 4312 1353 Size: 1442 148 655 714 3423 Fragments arranged by size: 3423 1442 714 655 148 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4969 5343 5489 387 Size: 1064 138 3380 374 146 1280 Fragments arranged by size: 3380 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2044 4445 5042 5829 6097 1239 Size: 393 412 2401 597 787 268 1524 Fragments arranged by size: 2401 1524 787 597 412 393 268 EcoO109I rG'GnC_Cy Cuts at: 2848 2848 Size: 6382 EcoRI G'AATT_C Cuts at: 3691 4069 3691 Size: 378 6004 EcoRV GAT'ATC Cuts at: 2821 2821 Size: 6382 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3581 3581 Size: 6382 FspI TGC'GCA Cuts at: 2009 4888 2009 Size: 2879 3503 HaeII r_GCGC'y Cuts at: 1678 5529 1678 Size: 3851 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2164 2196 2864 2896 3457 Size: 32 135 32 592 32 668 32 561 Cuts at: 3457 3489 5023 5055 5097 5129 1373 Size: 32 1534 32 42 32 2626 Fragments arranged by size: 2626 1534 668 592 561 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3251 3604 3726 3972 669 Size: 988 281 1313 353 122 246 3079 Fragments arranged by size: 3079 1313 988 353 281 246 122 HindIII A'AGCT_T Cuts at: 748 2243 748 Size: 1495 4887 HpaI GTT'AAC Cuts at: 3972 3972 Size: 6382 KpnI G_GTAC'C Cuts at: 3707 3707 Size: 6382 MfeI C'AATT_G Cuts at: 3293 3981 3293 Size: 688 5694 MluI A'CGCG_T Cuts at: 6258 6258 Size: 6382 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2422 5496 5680 1305 Size: 1117 3074 184 2007 Fragments arranged by size: 3074 2007 1117 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4560 Fragments arranged by size: 4560 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2312 2490 3204 4340 4699 4858 518 Size: 648 1146 178 714 1136 359 159 2042 Fragments arranged by size: 2042 1146 1136 714 648 359 178 159 MspA1I CmG'CkG Cuts at: 1606 2964 3798 4475 5623 5868 1606 Size: 1358 834 677 1148 245 2120 Fragments arranged by size: 2120 1358 1148 834 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5830 NdeI CA'TA_TG Cuts at: 387 2436 2778 387 Size: 2049 342 3991 Fragments arranged by size: 3991 2049 342 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6382 NotI GC'GGCC_GC Cuts at: 3829 3829 Size: 6382 NspI r_CATG'y Cuts at: 1132 1229 3740 5285 1132 Size: 97 2511 1545 2229 Fragments arranged by size: 2511 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6382 PciI A'CATG_T Cuts at: 5281 5281 Size: 6382 PflMI CCAn_nnn'nTGG Cuts at: 1108 3620 1108 Size: 2512 3870 PfoI T'CCnGG_A Cuts at: 2021 2021 Size: 6382 PmeI GTTT'AAAC Cuts at: 3686 3686 Size: 6382 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4451 4483 5964 5996 4451 Size: 32 1481 32 4837 Fragments arranged by size: 4837 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2848 2848 Size: 6382 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6382 PsiI TTA'TAA Cuts at: 3952 3952 Size: 6382 PstI C_TGCA'G Cuts at: 830 2787 3734 830 Size: 1957 947 3478 Fragments arranged by size: 3478 1957 947 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6382 PvuII CAG'CTG Cuts at: 2964 2964 Size: 6382 SalI G'TCGA_C Cuts at: 1936 3249 3724 1936 Size: 1313 475 4594 Fragments arranged by size: 4594 1313 475 SbfI CC_TGCA'GG Cuts at: 3734 3734 Size: 6382 ScaI AGT'ACT Cuts at: 4630 6342 4630 Size: 1712 4670 SexAI A'CCwGG_T Cuts at: 2277 2277 Size: 6382 SfcI C'TryA_G Cuts at: 826 1045 2783 3383 3730 4865 5546 5737 826 Size: 219 1738 600 347 1135 681 191 1471 Fragments arranged by size: 1738 1471 1135 681 600 347 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6382 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6382 SmaI CCC'GGG Cuts at: 1872 3709 1872 Size: 1837 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2240 3540 4486 5387 5649 820 Size: 197 926 297 1300 946 901 262 1553 Fragments arranged by size: 1553 1300 946 926 901 297 262 197 SnaBI TAC'GTA Cuts at: 493 4173 493 Size: 3680 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6382 SphI G_CATG'C Cuts at: 1132 3740 1132 Size: 2608 3774 SspI AAT'ATT Cuts at: 5 52 2744 4306 5 Size: 47 2692 1562 2081 Fragments arranged by size: 2692 2081 1562 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6382 StyI C'CwwG_G Cuts at: 513 1065 3152 3202 3821 513 Size: 552 2087 50 619 3074 Fragments arranged by size: 3074 2087 619 552 50 SwaI ATTT'AAAT Cuts at: 2748 2748 Size: 6382 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2081 2581 4409 4426 4579 4764 5209 Size: 1255 501 500 1828 17 153 185 445 Cuts at: 5209 325 Size: 1498 Fragments arranged by size: 1828 1498 1255 501 500 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2288 4628 6340 Size: 274 80 33 51 1443 311 2340 1712 Cuts at: 6340 96 Size: 138 Fragments arranged by size: 2340 1712 1443 311 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6342 6374 6342 Size: 32 6350 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6382 XbaI T'CTAG_A Cuts at: 3718 3718 Size: 6382 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6382 XmaI C'CCGG_G Cuts at: 1870 3707 1870 Size: 1837 4545 XmnI GAAnn'nnTTC Cuts at: 4511 4511 Size: 6382 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4190 276 Size: 53 83 186 936 2656 2468 Fragments arranged by size: 2656 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI XcmI