################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI AlwNI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoT22I FauI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI NarI NciI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tth111I TthHB8I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccII 675 CGCG CG^CG AciI 127 CCGC CCGC(-3/-1) AluI 371 AGCT AG^CT Alw44I 662 GTGCAC G^TGCAC AocI 465 CCTAAGG CC^T[ACGT]AGG ApaLI 662 GTGCAC G^TGCAC Asp700I 471 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 662 GTGCAC G[AT]GC[AT]^C BbvI 641 GCAGC GCAGC(8/12) BclI 104 TGATCA T^GATCA BmyI 662 GTGCAC G[AGT]GC[ACT]^C BsaBI 201 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 395 GGTCTC GGTCTC(1/5) BseRI 874 CTCCTC GAGGAG(10/8) BsiHKAI 662 GTGCAC G[AT]GC[AT]^C Bsp1286I 662 GTGCAC G[AGT]GC[ACT]^C BsrDI 557 CATTGC GCAATG(2/0) BsrFI 637 GCCGGC [GA]^CCGG[CT] BsrI 513 ACTGG ACTGG(1/-1) BstUI 675 CGCG CG^CG BstYI 132 GGATCT [GA]^GATC[CT] Bsu36I 465 CCTAAGG CC^T[ACGT]AGG CfoI 674 GCGC GCG^C Cfr10I 637 GCCGGC [GA]^CCGG[CT] DsaI 379 CCATGG C^C[GA][CT]GG EaeI 635 TGGCCG [CT]^GGCC[GA] Eco81I 465 CCTAAGG CC^T[ACGT]AGG EcoRI 220 GAATTC G^AATTC EcoRV 363 GATATC GAT^ATC FbaI 104 TGATCA T^GATCA Fnu4HI 641 GCAGC GC^[ACGT]GC HaeIII 636 GGCC GG^CC HapII 638 CCGG C^CGG HgaI 326 GCGTC GACGC(5/10) HhaI 674 GCGC GCG^C HinP1I 674 GCGC G^CGC HinfI 7 GAGTC G^A[ACGT]TC HpaII 638 CCGG C^CGG ItaI 641 GCAGC GC^[ACGT]GC MaeII 191 ACGT A^CGT MaeIII 332 GTCAC ^GT[ACGT]AC MamI 201 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 132 GGATCT [GA]^GATC[CT] MspA1I 127 CCGCTG C[AC]G^C[GT]G MspI 638 CCGG C^CGG MvnI 675 CGCG CG^CG NaeI 637 GCCGGC GCC^GGC NcoI 379 CCATGG C^CATGG NgoMIV 637 GCCGGC G^CCGGC PleI 7 GAGTC GAGTC(4/5) PstI 172 CTGCAG CTGCA^G SfaNI 74 GCATC GCATC(5/9) SfcI 172 CTGCAG C^T[GA][CT]AG TseI 641 GCAGC G^C[AT]GC Tsp45I 332 GTCAC ^GT[GC]AC XbaI 160 TCTAGA T^CTAGA XcmI 337 CCACTCTTCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 132 GGATCT [GA]^GATC[CT] XmnI 471 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AvaII 375 GGTCC G^G[AT]CC 453 GGTCC BfaI 4 CTAG C^TAG 161 CTAG BsiYI 638 CCGGCAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 652 CCTGGCAGGGG BslI 638 CCGGCAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 652 CCTGGCAGGGG BsmAI 9 GTCTC GTCTC(1/5) 396 GTCTC BsmFI 376 GTCCC GGGAC(10/14) 454 GTCCC BstF5I 786 CATCC GGATG(2/0) 383 GGATG Cfr13I 375 GGTCC G^G[ACGT]CC 453 GGTCC EarI 340 CTCTTC CTCTTC(1/4) 741 CTCTTC EcoT14I 314 CCAAGG C^C[AT][AT]GG 379 CCATGG FokI 786 CATCC GGATG(9/13) 383 GGATG Ksp632I 340 CTCTTC CTCTTC(1/4) 741 CTCTTC MaeI 4 CTAG C^TAG 161 CTAG MseI 194 TTAA T^TAA 829 TTAA Sau96I 375 GGTCC G^G[ACGT]CC 453 GGTCC StyI 314 CCAAGG C^C[AT][AT]GG 379 CCATGG Tru9I 194 TTAA T^TAA 829 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AcsI AlwI ApoI BsaJI BssKI BstNI Cac8I DdeI DpnII EcoRII HphI MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII NlaIV Sau3AI ScrFI Tsp509I TspRI