################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AspEI AspHI AvaI AvrII BalI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI HaeII HgaI HincII HindII HindIII HpaI KasI KspI MamI MluI MluNI MroI MscI MvnI NaeI NarI NcoI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI Tsp45I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### Acc65I 813 GGTACC G^GTACC AseI 721 ATTAAT AT^TAAT AsnI 721 ATTAAT AT^TAAT Asp700I 768 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 813 GGTACC G^GTACC AspI 289 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 673 TGCGCA TGC^GCA BamHI 835 GGATCC G^GATCC BanI 813 GGTACC G^G[CT][GA]CC BbvI 231 GCTGC GCAGC(8/12) BclI 86 TGATCA T^GATCA BcnI 693 CCCGG CC^[GC]GG BglI 234 GCCTGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 751 GTGCCC G[AGT]GC[ACT]^C BseRI 371 GAGGAG GAGGAG(10/8) BsmI 136 GAATGC GAATGC(1/-1) Bsp1286I 751 GTGCCC G[AGT]GC[ACT]^C Bsp1407I 565 TGTACA T^GTACA BsrDI 763 GCAATG GCAATG(2/0) BsrGI 565 TGTACA T^GTACA BstYI 835 GGATCC [GA]^GATC[CT] CfoI 674 GCGC GCG^C DraII 5 GGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 643 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 441 CCACGG C^C[GA][CT]GG EaeI 107 CGGCCA [CT]^GGCC[GA] EarI 49 CTCTTC CTCTTC(1/4) EcoO109I 5 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoT14I 129 CCAAGG C^C[AT][AT]GG FbaI 86 TGATCA T^GATCA FspI 673 TGCGCA TGC^GCA HhaI 674 GCGC GCG^C HinP1I 674 GCGC G^CGC HinfI 446 GAATC G^A[ACGT]TC KpnI 813 GGTACC GGTAC^C Ksp632I 49 CTCTTC CTCTTC(1/4) MaeIII 577 GTAAC ^GT[ACGT]AC MfeI 561 CAATTG C^AATTG MflI 835 GGATCC [GA]^GATC[CT] MseI 722 TTAA T^TAA MslI 186 CACCGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 561 CAATTG C^AATTG NciI 693 CCCGG CC^[GC]GG NdeI 581 CATATG CA^TATG PflFI 289 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 721 ATTAAT AT^TAAT PvuII 56 CAGCTG CAG^CTG SapI 48 GCTCTTC GCTCTTC(1/4) SfcI 222 CTACAG C^T[GA][CT]AG SspBI 565 TGTACA T^GTACA StuI 599 AGGCCT AGG^CCT StyI 129 CCAAGG C^C[AT][AT]GG TfiI 446 GAATC G^A[AT]TC Tru9I 722 TTAA T^TAA TseI 231 GCTGC G^C[AT]GC TspRI 150 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 289 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 835 GGATCC [GA]^GATC[CT] XmnI 768 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AatII 144 GACGTC GACGT^C 631 GACGTC AcyI 144 GACGTC G[GA]^CG[CT]C 631 GACGTC BfaI 332 CTAG C^TAG 574 CTAG BpmI 121 CTCCAG CTGGAG(16/14) 368 CTGGAG BsaHI 144 GACGTC G[GA]^CG[CT]C 631 GACGTC BsrI 367 ACTGG ACTGG(1/-1) 149 CCAGT BstF5I 212 GGATG GGATG(2/0) 699 GGATG DdeI 54 CTCAG C^T[ACGT]AG 271 CTCAG EcoNI 266 CCTCTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 331 CCTAGTTTAGG FokI 212 GGATG GGATG(9/13) 699 GGATG HapII 117 CCGG C^CGG 694 CCGG Hin1I 144 GACGTC G[GA]^CG[CT]C 631 GACGTC HpaII 117 CCGG C^CGG 694 CCGG MaeI 332 CTAG C^TAG 574 CTAG MboII 50 TCTTC GAAGA(8/7) 800 TCTTC MspI 117 CCGG C^CGG 694 CCGG Tsp509I 562 AATT ^AATT 724 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BsaJI BsiYI BslI BsmFI BssKI BstNI Cac8I Cfr13I DpnII EcoRII FauI Fnu4HI HaeIII HphI ItaI MaeII MboI MnlI MspA1I MvaI MwoI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI