(Circular) (Six-base) MAPSORT of: pFN21AE5359.seq Check: 917 from: 1 to: 5626 pFN21AE5359 5626 bp With 182 enzymes: SgfI * January 30, 2014 17:18 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2153 2640 3436 278 Size: 53 83 186 936 617 487 796 2468 Fragments arranged by size: 2468 936 796 617 487 186 83 53 AccI GT'mk_AC Cuts at: 1937 2969 1937 Size: 1032 4594 Acc65I G'GTAC_C Cuts at: 2818 2947 2818 Size: 129 5497 AclI AA'CG_TT Cuts at: 1563 3753 4126 1563 Size: 2190 373 3063 Fragments arranged by size: 3063 2190 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2234 3689 5073 5341 1632 Size: 602 1455 1384 268 1917 Fragments arranged by size: 1917 1455 1384 602 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5429 AflIII A'CryG_T Cuts at: 1203 4525 5502 1203 Size: 3322 977 1327 Fragments arranged by size: 3322 1327 977 AgeI A'CCGG_T Cuts at: 4457 4457 Size: 5626 AhdI GACnn_n'nnGTC Cuts at: 4355 4355 Size: 5626 AleI CACnn'nnGTG Cuts at: 1166 2195 1166 Size: 1029 4597 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5626 AlwNI CAG_nnn'CTG Cuts at: 3369 4941 3369 Size: 1572 4054 ApaLI G'TGCA_C Cuts at: 3683 4839 3683 Size: 1156 4470 ApoI r'AATT_y Cuts at: 1493 2935 3161 3313 5422 1493 Size: 1442 226 152 2109 1697 Fragments arranged by size: 2109 1697 1442 226 152 AseI AT'TA_AT Cuts at: 160 1032 2727 4180 160 Size: 872 1695 1453 1606 Fragments arranged by size: 1695 1606 1453 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 AvaI C'yCGr_G Cuts at: 1870 1943 2951 1870 Size: 73 1008 4545 Fragments arranged by size: 4545 1008 73 BamHI G'GATC_C Cuts at: 2840 2956 3323 2840 Size: 116 367 5143 Fragments arranged by size: 5143 367 116 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2818 2947 4402 618 Size: 325 217 627 1031 129 1455 1842 Fragments arranged by size: 1842 1455 1031 627 325 217 129 BanII G_rGCy'C Cuts at: 1391 2945 1391 Size: 1554 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4655 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3817 3851 1903 Size: 34 1880 34 3678 Fragments arranged by size: 3678 1880 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3518 4728 3518 Size: 1210 4416 BclI T'GATC_A Cuts at: 1548 2091 1548 Size: 543 5083 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2245 4237 136 Size: 107 122 71 631 605 573 1992 1525 Fragments arranged by size: 1992 1525 631 605 573 122 107 71 BglII A'GATC_T Cuts at: 5621 5621 Size: 5626 BlpI GC'TnA_GC Cuts at: 3043 3043 Size: 5626 Bme1580I G_kGCm'C Cuts at: 1790 2760 3687 4843 1790 Size: 970 927 1156 2573 Fragments arranged by size: 2573 1156 970 927 BmgBI CAC'GTC Cuts at: 1318 2089 1318 Size: 771 4855 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3357 4315 442 Size: 416 53 83 399 24 1940 958 1753 Fragments arranged by size: 1940 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5626 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2111 2394 4286 1239 Size: 872 283 1892 2579 Fragments arranged by size: 2579 1892 872 283 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3751 4616 4914 3751 Size: 865 298 4463 Fragments arranged by size: 4463 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4289 882 Size: 617 2790 2219 Fragments arranged by size: 2790 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3417 493 Size: 711 2213 2702 Fragments arranged by size: 2702 2213 711 BsaBI GATnn'nnATC Cuts at: 5620 5620 Size: 5626 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2150 2637 3433 3815 Size: 53 83 186 936 617 487 796 382 Cuts at: 3815 4450 275 Size: 635 1451 Fragments arranged by size: 1451 936 796 635 617 487 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4059 4457 4731 4878 5289 4059 Size: 398 274 147 411 4396 Fragments arranged by size: 4396 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2370 2400 1178 Size: 30 165 30 967 30 4404 Fragments arranged by size: 4404 967 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2391 1186 Size: 1205 4421 BseYI C'CCAG_C Cuts at: 4829 5325 4829 Size: 496 5130 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5053 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3076 3837 4865 1540 Size: 275 186 1075 761 1028 2301 Fragments arranged by size: 2301 1075 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3687 3772 4843 3687 Size: 85 1071 4470 Fragments arranged by size: 4470 1071 85 BsmI GAATG_Cn' Cuts at: 2147 3137 3230 2147 Size: 990 93 4543 Fragments arranged by size: 4543 990 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2760 2945 3687 3772 4843 1391 Size: 399 970 185 742 85 1071 2174 Fragments arranged by size: 2174 1071 970 742 399 185 85 BspEI T'CCGG_A Cuts at: 5289 5289 Size: 5626 BspHI T'CATG_A Cuts at: 3515 5245 3515 Size: 1730 3896 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2981 844 Size: 2137 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3513 1394 Size: 429 1690 3507 Fragments arranged by size: 3507 1690 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2775 4121 4295 65 Size: 1183 1527 1346 174 1396 Fragments arranged by size: 1527 1396 1346 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4270 4457 1700 Size: 110 2460 187 2869 Fragments arranged by size: 2869 2460 187 110 BsrGI T'GTAC_A Cuts at: 96 2570 96 Size: 2474 3152 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5626 BssSI C'ACGA_G Cuts at: 3686 4698 3686 Size: 1012 4614 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5626 BstBI TT'CG_AA Cuts at: 4430 4430 Size: 5626 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5626 BstYI r'GATC_y Cuts at: 1972 2840 2956 3323 3708 3725 5177 5263 5275 Size: 868 116 367 385 17 1452 86 12 Cuts at: 5275 5621 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 868 385 367 346 116 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2446 513 Size: 552 939 442 3693 Fragments arranged by size: 3693 939 552 442 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4887 Fragments arranged by size: 4887 683 56 BtsI GCAGTG_nn' Cuts at: 3142 3936 3956 3142 Size: 794 20 4812 Fragments arranged by size: 4812 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5626 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5591 DraI TTT'AAA Cuts at: 2930 2930 Size: 5626 DraIII CAC_nnn'GTG Cuts at: 2653 2653 Size: 5626 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4633 809 Size: 507 3317 1802 Fragments arranged by size: 3317 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2112 3073 3962 Size: 54 1425 50 275 18 282 961 889 Cuts at: 3962 5591 8 Size: 1629 43 Fragments arranged by size: 1629 1425 961 889 282 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3073 1537 Size: 275 1261 4090 Fragments arranged by size: 4090 1261 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2060 3556 1353 Size: 707 1496 3423 Fragments arranged by size: 3423 1496 707 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2099 2230 2257 4213 4587 4733 Size: 1064 138 510 131 27 1956 374 146 Cuts at: 4733 387 Size: 1280 Fragments arranged by size: 1956 1280 1064 510 374 146 138 131 27 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2234 2394 3689 4286 5073 5341 Size: 393 479 123 160 1295 597 787 268 Cuts at: 5341 1239 Size: 1524 Fragments arranged by size: 1524 1295 787 597 479 393 268 160 123 EcoNI CCTnn'n_nnAGG Cuts at: 2275 2340 2275 Size: 65 5561 EcoO109I rG'GnC_Cy Cuts at: 2011 2011 Size: 5626 EcoRI G'AATT_C Cuts at: 2935 3313 2935 Size: 378 5248 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2441 2623 2441 Size: 182 5444 FspI TGC'GCA Cuts at: 2680 4132 2680 Size: 1452 4174 HaeII r_GCGC'y Cuts at: 1678 4773 1678 Size: 3095 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4267 4299 4341 4373 1373 Size: 32 135 32 2695 32 42 32 2626 Fragments arranged by size: 2695 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2970 3216 669 Size: 988 281 1032 246 3079 Fragments arranged by size: 3079 1032 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5626 HpaI GTT'AAC Cuts at: 3216 3216 Size: 5626 KpnI G_GTAC'C Cuts at: 2822 2951 2822 Size: 129 5497 MfeI C'AATT_G Cuts at: 2566 3225 2566 Size: 659 4967 MluI A'CGCG_T Cuts at: 5502 5502 Size: 5626 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4740 4924 1305 Size: 3435 184 2007 Fragments arranged by size: 3435 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3804 Fragments arranged by size: 3804 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2195 3584 3943 4102 518 Size: 648 1029 1389 359 159 2042 Fragments arranged by size: 2042 1389 1029 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2063 2104 2195 2232 2254 3042 3719 4867 Size: 457 41 91 37 22 788 677 1148 Cuts at: 4867 5112 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 788 677 457 245 91 41 37 22 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4135 Fragments arranged by size: 4135 939 552 NdeI CA'TA_TG Cuts at: 387 2587 387 Size: 2200 3426 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5626 NotI GC'GGCC_GC Cuts at: 3073 3073 Size: 5626 NspI r_CATG'y Cuts at: 1132 1229 2984 4529 1132 Size: 97 1755 1545 2229 Fragments arranged by size: 2229 1755 1545 97 PasI CC'CwG_GG Cuts at: 1348 2041 1348 Size: 693 4933 PciI A'CATG_T Cuts at: 4525 4525 Size: 5626 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5626 PfoI T'CCnGG_A Cuts at: 2301 2843 2301 Size: 542 5084 PmeI GTTT'AAAC Cuts at: 2930 2930 Size: 5626 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3695 3727 5208 5240 3695 Size: 32 1481 32 4081 Fragments arranged by size: 4081 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5626 PsiI TTA'TAA Cuts at: 2478 3196 2478 Size: 718 4908 PstI C_TGCA'G Cuts at: 830 2978 830 Size: 2148 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5626 PvuII CAG'CTG Cuts at: 2063 2063 Size: 5626 SalI G'TCGA_C Cuts at: 1936 2968 1936 Size: 1032 4594 SapI GCTCTTCn'nnn_ Cuts at: 2060 2060 Size: 5626 SbfI CC_TGCA'GG Cuts at: 2978 2978 Size: 5626 ScaI AGT'ACT Cuts at: 3874 5586 3874 Size: 1712 3914 SfcI C'TryA_G Cuts at: 826 1045 2227 2974 4109 4790 4981 826 Size: 219 1182 747 1135 681 191 1471 Fragments arranged by size: 1471 1182 1135 747 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5626 SmaI CCC'GGG Cuts at: 1872 2953 1872 Size: 1081 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3730 4631 4893 820 Size: 197 926 1787 901 262 1553 Fragments arranged by size: 1787 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3417 493 Size: 2924 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5626 SphI G_CATG'C Cuts at: 1132 2984 1132 Size: 1852 3774 SspI AAT'ATT Cuts at: 5 52 3550 5 Size: 47 3498 2081 Fragments arranged by size: 3498 2081 47 StuI AGG'CCT Cuts at: 1517 2606 1517 Size: 1089 4537 StyI C'CwwG_G Cuts at: 513 1065 2004 2134 3065 513 Size: 552 939 130 931 3074 Fragments arranged by size: 3074 939 931 552 130 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3653 3670 3823 4008 4453 325 Size: 1255 2073 17 153 185 445 1498 Fragments arranged by size: 2073 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2570 2745 3872 Size: 274 80 33 51 1443 593 175 1127 Cuts at: 3872 5584 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1127 593 274 175 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5586 5618 5586 Size: 32 5594 Tth111I GACn'n_nGTC Cuts at: 1316 2297 1316 Size: 981 4645 XbaI T'CTAG_A Cuts at: 2962 2962 Size: 5626 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5626 XmaI C'CCGG_G Cuts at: 1870 2951 1870 Size: 1081 4545 XmnI GAAnn'nnTTC Cuts at: 2777 3755 2777 Size: 978 4648 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2151 2638 3434 276 Size: 53 83 186 936 617 487 796 2468 Fragments arranged by size: 2468 936 796 617 487 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI