Restriction map for pFN21AE1605
 (Circular) (Six-base) MAPSORT of: pFN21AE1605.seq  Check: 993  from: 1  to: 5032



pFN21AE1605  5032 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:52  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2842     278 
   Size:         53      83     186     936    1306    2468

  Fragments arranged by size: 

                2468    1306     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2375    1937 
   Size:        438    4594
 
Acc65I G'GTAC_C

Cuts at:   2353    2353 
   Size:       5032
 
AclI AA'CG_TT

Cuts at:   1563    3159    3532    1563 
   Size:       1596     373    3063

  Fragments arranged by size: 

                3063    1596     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3095    4479    4747    1632 
   Size:       1463    1384     268    1917

  Fragments arranged by size: 

                1917    1463    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4835
 
AflIII A'CryG_T

Cuts at:   1203    3931    4908    1203 
   Size:       2728     977    1327

  Fragments arranged by size: 

                2728    1327     977
 
AgeI A'CCGG_T

Cuts at:   3863    3863 
   Size:       5032
 
AhdI GACnn_n'nnGTC

Cuts at:   3761    3761 
   Size:       5032
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5032
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5032
 
AlwNI CAG_nnn'CTG

Cuts at:   2775    4347    2775 
   Size:       1572    3460
 
ApaLI G'TGCA_C

Cuts at:   3089    4245    3089 
   Size:       1156    3876
 
ApoI r'AATT_y

Cuts at:   1493    2154    2341    2567    2719    4828    1493 
   Size:        661     187     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     661     226     187     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3586     160 
   Size:        872    2554    1606

  Fragments arranged by size: 

                2554    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5032
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2357    1870 
   Size:         73     414    4545

  Fragments arranged by size: 

                4545     414      73
 
BamHI G'GATC_C

Cuts at:   2362    2729    2362 
   Size:        367    4665
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2353    3808     618 
   Size:        325     217     627     566    1455    1842

  Fragments arranged by size: 

                1842    1455     627     566     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2123    2351    1391 
   Size:        732     228    4072

  Fragments arranged by size: 

                4072     732     228
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4061
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2008    2042    3223    3257    1903 
   Size:         34      71      34    1181      34    3678

  Fragments arranged by size: 

                3678    1181      71      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2247    2924    4134    2247 
   Size:        677    1210    3145

  Fragments arranged by size: 

                3145    1210     677
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5032
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3643     136 
   Size:        107     122      71     631     605    1971    1525

  Fragments arranged by size: 

                1971    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5027    5027 
   Size:       5032
 
BlpI GC'TnA_GC

Cuts at:   2449    2449 
   Size:       5032
 
Bme1580I G_kGCm'C

Cuts at:   1790    3093    4249    1790 
   Size:       1303    1156    2573

  Fragments arranged by size: 

                2573    1303    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5032
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2763    3721     442 
   Size:        416      53      83     399      24    1346     958    1753

  Fragments arranged by size: 

                1753    1346     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5032
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2043    2096    3692    1239 
   Size:        804      53    1596    2579

  Fragments arranged by size: 

                2579    1596     804      53
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2116    2226    3157    4022    4320    2116 
   Size:        110     931     865     298    2828

  Fragments arranged by size: 

                2828     931     865     298     110
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3695     882 
   Size:        617    2196    2219

  Fragments arranged by size: 

                2219    2196     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2823     493 
   Size:        711    1619    2702

  Fragments arranged by size: 

                2702    1619     711
 
BsaBI GATnn'nnATC

Cuts at:   5026    5026 
   Size:       5032
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2839    3221    3856     275 
   Size:         53      83     186     936    1306     382     635    1451

  Fragments arranged by size: 

                1451    1306     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3465    3863    4137    4284    4695    3465 
   Size:        398     274     147     411    3802

  Fragments arranged by size: 

                3802     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4807

  Fragments arranged by size: 

                4807     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5032
 
BseYI C'CCAG_C

Cuts at:   4235    4731    4235 
   Size:        496    4536
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4459
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2482    3243    4271    1540 
   Size:        275     186     481     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     481     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2123    3093    3178    4249    2123 
   Size:        970      85    1071    2906

  Fragments arranged by size: 

                2906    1071     970      85
 
BsmI GAATG_Cn'

Cuts at:   2543    2636    2543 
   Size:         93    4939
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2123    2351    3093    3178    4249    1391 
   Size:        399     333     228     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     333     228      85
 
BspEI T'CCGG_A

Cuts at:   4695    4695 
   Size:       5032
 
BspHI T'CATG_A

Cuts at:   2921    4651    2921 
   Size:       1730    3302
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2387     844 
   Size:       1543    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2919    1394 
   Size:        429    1096    3507

  Fragments arranged by size: 

                3507    1096     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3527    3701      65 
   Size:       1183    2279     174    1396

  Fragments arranged by size: 

                2279    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3676    3863    1700 
   Size:        110    1866     187    2869

  Fragments arranged by size: 

                2869    1866     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5032
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5032
 
BssSI C'ACGA_G

Cuts at:   3092    4104    3092 
   Size:       1012    4020
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5032
 
BstBI TT'CG_AA

Cuts at:   3836    3836 
   Size:       5032
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5032
 
BstYI r'GATC_y

Cuts at:   1972    2362    2729    3114    3131    4583    4669    4681    5027 
   Size:        390     367     385      17    1452      86      12     346

Cuts at:   5027    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     390     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2170     513 
   Size:        552    1105    3375

  Fragments arranged by size: 

                3375    1105     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4293

  Fragments arranged by size: 

                4293     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2548    3342    3362    2548 
   Size:        794      20    4218

  Fragments arranged by size: 

                4218     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5032
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    4997
 
DraI TTT'AAA

Cuts at:   2336    2336 
   Size:       5032
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4039     809 
   Size:        507    2723    1802

  Fragments arranged by size: 

                2723    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2479    3368    4997 
   Size:         54    1425      50     275      18     649     889    1629

Cuts at:   4997       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     649     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2479    1537 
   Size:        275     667    4090

  Fragments arranged by size: 

                4090     667     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2962    1353 
   Size:       1609    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3619    3993    4139     387 
   Size:       1064     138    2030     374     146    1280

  Fragments arranged by size: 

                2030    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2121    2121 
   Size:       5032
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2043    2096    3095    3692    4479    4747    1239 
   Size:        393     411      53     999     597     787     268    1524

  Fragments arranged by size: 

                1524     999     787     597     411     393     268      53
            
 
EcoRI G'AATT_C

Cuts at:   2154    2341    2719    2154 
   Size:        187     378    4467

  Fragments arranged by size: 

                4467     378     187
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2307    2307 
   Size:       5032
 
FspI TGC'GCA

Cuts at:   3538    3538 
   Size:       5032
 
HaeII r_GCGC'y

Cuts at:   1678    4179    1678 
   Size:       2501    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3673    3705    3747    3779    1373 
   Size:         32     135      32    2101      32      42      32    2626

  Fragments arranged by size: 

                2626    2101     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2376    2622     669 
   Size:        988     281     438     246    3079

  Fragments arranged by size: 

                3079     988     438     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2092     748 
   Size:       1344    3688
 
HpaI GTT'AAC

Cuts at:   2622    2622 
   Size:       5032
 
KpnI G_GTAC'C

Cuts at:   2357    2357 
   Size:       5032
 
MfeI C'AATT_G

Cuts at:   2223    2631    2223 
   Size:        408    4624
 
MluI A'CGCG_T

Cuts at:   4908    4908 
   Size:       5032
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4146    4330    1305 
   Size:       2841     184    2007

  Fragments arranged by size: 

                2841    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3210

  Fragments arranged by size: 

                3210    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2990    3349    3508     518 
   Size:        648    1824     359     159    2042

  Fragments arranged by size: 

                2042    1824     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2448    3125    4273    4518    1606 
   Size:        842     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     842     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4480
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5032
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5032
 
NotI GC'GGCC_GC

Cuts at:   2479    2479 
   Size:       5032
 
NspI r_CATG'y

Cuts at:   1132    1229    2390    3935    1132 
   Size:         97    1161    1545    2229

  Fragments arranged by size: 

                2229    1545    1161      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5032
 
PciI A'CATG_T

Cuts at:   3931    3931 
   Size:       5032
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5032
 
PmeI GTTT'AAAC

Cuts at:   2336    2336 
   Size:       5032
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3101    3133    4614    4646    3101 
   Size:         32    1481      32    3487

  Fragments arranged by size: 

                3487    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5032
 
PsiI TTA'TAA

Cuts at:   2602    2602 
   Size:       5032
 
PstI C_TGCA'G

Cuts at:    830    2384     830 
   Size:       1554    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5032
 
SacI G_AGCT'C

Cuts at:   2123    2123 
   Size:       5032
 
SalI G'TCGA_C

Cuts at:   1936    2374    1936 
   Size:        438    4594
 
SbfI CC_TGCA'GG

Cuts at:   2384    2384 
   Size:       5032
 
ScaI AGT'ACT

Cuts at:   3280    4992    3280 
   Size:       1712    3320
 
SfcI C'TryA_G

Cuts at:    826    1045    2380    3515    4196    4387     826 
   Size:        219    1335    1135     681     191    1471

  Fragments arranged by size: 

                1471    1335    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5032
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5032
 
SmaI CCC'GGG

Cuts at:   1872    2359    1872 
   Size:        487    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2095    2241    3136    4037    4299     820 
   Size:        197     926     152     146     895     901     262    1553

  Fragments arranged by size: 

                1553     926     901     895     262     197     152     146
            
 
SnaBI TAC'GTA

Cuts at:    493    2823     493 
   Size:       2330    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5032
 
SphI G_CATG'C

Cuts at:   1132    2390    1132 
   Size:       1258    3774
 
SspI AAT'ATT

Cuts at:      5      52    2956       5 
   Size:         47    2904    2081

  Fragments arranged by size: 

                2904    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5032
 
StyI C'CwwG_G

Cuts at:    513    1065    2065    2471     513 
   Size:        552    1000     406    3074

  Fragments arranged by size: 

                3074    1000     552     406
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3059    3076    3229    3414    3859     325 
   Size:       1255    1479      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1479    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3278    4990      96 
   Size:        274      80      33      51    1443    1301    1712     138

  Fragments arranged by size: 

                1712    1443    1301     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4992    5024    4992 
   Size:         32    5000
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5032
 
XbaI T'CTAG_A

Cuts at:   2368    2368 
   Size:       5032
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5032
 
XmaI C'CCGG_G

Cuts at:   1870    2357    1870 
   Size:        487    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2290    3161    2290 
   Size:        871    4161
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2840     276 
   Size:         53      83     186     936    1306    2468

  Fragments arranged by size: 

                2468    1306     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI
     PpiI    PshAI     PsiI     PstI     PvuI     SacI     SalI     SbfI
     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I   DraIII    EcoNI EcoO109I    EcoRV     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI
    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII    SacII    SanDI
     SapI    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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