(Circular) (Six-base) MAPSORT of: pFN21AE1605.seq Check: 993 from: 1 to: 5032 pFN21AE1605 5032 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2842 278 Size: 53 83 186 936 1306 2468 Fragments arranged by size: 2468 1306 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2375 1937 Size: 438 4594 Acc65I G'GTAC_C Cuts at: 2353 2353 Size: 5032 AclI AA'CG_TT Cuts at: 1563 3159 3532 1563 Size: 1596 373 3063 Fragments arranged by size: 3063 1596 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3095 4479 4747 1632 Size: 1463 1384 268 1917 Fragments arranged by size: 1917 1463 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4835 AflIII A'CryG_T Cuts at: 1203 3931 4908 1203 Size: 2728 977 1327 Fragments arranged by size: 2728 1327 977 AgeI A'CCGG_T Cuts at: 3863 3863 Size: 5032 AhdI GACnn_n'nnGTC Cuts at: 3761 3761 Size: 5032 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5032 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5032 AlwNI CAG_nnn'CTG Cuts at: 2775 4347 2775 Size: 1572 3460 ApaLI G'TGCA_C Cuts at: 3089 4245 3089 Size: 1156 3876 ApoI r'AATT_y Cuts at: 1493 2154 2341 2567 2719 4828 1493 Size: 661 187 226 152 2109 1697 Fragments arranged by size: 2109 1697 661 226 187 152 AseI AT'TA_AT Cuts at: 160 1032 3586 160 Size: 872 2554 1606 Fragments arranged by size: 2554 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5032 AvaI C'yCGr_G Cuts at: 1870 1943 2357 1870 Size: 73 414 4545 Fragments arranged by size: 4545 414 73 BamHI G'GATC_C Cuts at: 2362 2729 2362 Size: 367 4665 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2353 3808 618 Size: 325 217 627 566 1455 1842 Fragments arranged by size: 1842 1455 627 566 325 217 BanII G_rGCy'C Cuts at: 1391 2123 2351 1391 Size: 732 228 4072 Fragments arranged by size: 4072 732 228 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4061 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2008 2042 3223 3257 1903 Size: 34 71 34 1181 34 3678 Fragments arranged by size: 3678 1181 71 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2247 2924 4134 2247 Size: 677 1210 3145 Fragments arranged by size: 3145 1210 677 BclI T'GATC_A Cuts at: 1548 1548 Size: 5032 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3643 136 Size: 107 122 71 631 605 1971 1525 Fragments arranged by size: 1971 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5027 5027 Size: 5032 BlpI GC'TnA_GC Cuts at: 2449 2449 Size: 5032 Bme1580I G_kGCm'C Cuts at: 1790 3093 4249 1790 Size: 1303 1156 2573 Fragments arranged by size: 2573 1303 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5032 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2763 3721 442 Size: 416 53 83 399 24 1346 958 1753 Fragments arranged by size: 1753 1346 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5032 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2043 2096 3692 1239 Size: 804 53 1596 2579 Fragments arranged by size: 2579 1596 804 53 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2116 2226 3157 4022 4320 2116 Size: 110 931 865 298 2828 Fragments arranged by size: 2828 931 865 298 110 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3695 882 Size: 617 2196 2219 Fragments arranged by size: 2219 2196 617 BsaAI yAC'GTr Cuts at: 493 1204 2823 493 Size: 711 1619 2702 Fragments arranged by size: 2702 1619 711 BsaBI GATnn'nnATC Cuts at: 5026 5026 Size: 5032 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2839 3221 3856 275 Size: 53 83 186 936 1306 382 635 1451 Fragments arranged by size: 1451 1306 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3465 3863 4137 4284 4695 3465 Size: 398 274 147 411 3802 Fragments arranged by size: 3802 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4807 Fragments arranged by size: 4807 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5032 BseYI C'CCAG_C Cuts at: 4235 4731 4235 Size: 496 4536 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4459 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2482 3243 4271 1540 Size: 275 186 481 761 1028 2301 Fragments arranged by size: 2301 1028 761 481 275 186 BsiHKAI G_wGCw'C Cuts at: 2123 3093 3178 4249 2123 Size: 970 85 1071 2906 Fragments arranged by size: 2906 1071 970 85 BsmI GAATG_Cn' Cuts at: 2543 2636 2543 Size: 93 4939 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2123 2351 3093 3178 4249 1391 Size: 399 333 228 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 333 228 85 BspEI T'CCGG_A Cuts at: 4695 4695 Size: 5032 BspHI T'CATG_A Cuts at: 2921 4651 2921 Size: 1730 3302 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2387 844 Size: 1543 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2919 1394 Size: 429 1096 3507 Fragments arranged by size: 3507 1096 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3527 3701 65 Size: 1183 2279 174 1396 Fragments arranged by size: 2279 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3676 3863 1700 Size: 110 1866 187 2869 Fragments arranged by size: 2869 1866 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5032 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5032 BssSI C'ACGA_G Cuts at: 3092 4104 3092 Size: 1012 4020 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5032 BstBI TT'CG_AA Cuts at: 3836 3836 Size: 5032 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5032 BstYI r'GATC_y Cuts at: 1972 2362 2729 3114 3131 4583 4669 4681 5027 Size: 390 367 385 17 1452 86 12 346 Cuts at: 5027 1972 Size: 1977 Fragments arranged by size: 1977 1452 390 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2170 513 Size: 552 1105 3375 Fragments arranged by size: 3375 1105 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4293 Fragments arranged by size: 4293 683 56 BtsI GCAGTG_nn' Cuts at: 2548 3342 3362 2548 Size: 794 20 4218 Fragments arranged by size: 4218 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5032 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4997 DraI TTT'AAA Cuts at: 2336 2336 Size: 5032 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4039 809 Size: 507 2723 1802 Fragments arranged by size: 2723 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2479 3368 4997 Size: 54 1425 50 275 18 649 889 1629 Cuts at: 4997 8 Size: 43 Fragments arranged by size: 1629 1425 889 649 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2479 1537 Size: 275 667 4090 Fragments arranged by size: 4090 667 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2962 1353 Size: 1609 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3619 3993 4139 387 Size: 1064 138 2030 374 146 1280 Fragments arranged by size: 2030 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2121 2121 Size: 5032 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2043 2096 3095 3692 4479 4747 1239 Size: 393 411 53 999 597 787 268 1524 Fragments arranged by size: 1524 999 787 597 411 393 268 53 EcoRI G'AATT_C Cuts at: 2154 2341 2719 2154 Size: 187 378 4467 Fragments arranged by size: 4467 378 187 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2307 2307 Size: 5032 FspI TGC'GCA Cuts at: 3538 3538 Size: 5032 HaeII r_GCGC'y Cuts at: 1678 4179 1678 Size: 2501 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3673 3705 3747 3779 1373 Size: 32 135 32 2101 32 42 32 2626 Fragments arranged by size: 2626 2101 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2376 2622 669 Size: 988 281 438 246 3079 Fragments arranged by size: 3079 988 438 281 246 HindIII A'AGCT_T Cuts at: 748 2092 748 Size: 1344 3688 HpaI GTT'AAC Cuts at: 2622 2622 Size: 5032 KpnI G_GTAC'C Cuts at: 2357 2357 Size: 5032 MfeI C'AATT_G Cuts at: 2223 2631 2223 Size: 408 4624 MluI A'CGCG_T Cuts at: 4908 4908 Size: 5032 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4146 4330 1305 Size: 2841 184 2007 Fragments arranged by size: 2841 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3210 Fragments arranged by size: 3210 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2990 3349 3508 518 Size: 648 1824 359 159 2042 Fragments arranged by size: 2042 1824 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2448 3125 4273 4518 1606 Size: 842 677 1148 245 2120 Fragments arranged by size: 2120 1148 842 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4480 NdeI CA'TA_TG Cuts at: 387 387 Size: 5032 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5032 NotI GC'GGCC_GC Cuts at: 2479 2479 Size: 5032 NspI r_CATG'y Cuts at: 1132 1229 2390 3935 1132 Size: 97 1161 1545 2229 Fragments arranged by size: 2229 1545 1161 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5032 PciI A'CATG_T Cuts at: 3931 3931 Size: 5032 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5032 PmeI GTTT'AAAC Cuts at: 2336 2336 Size: 5032 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3101 3133 4614 4646 3101 Size: 32 1481 32 3487 Fragments arranged by size: 3487 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5032 PsiI TTA'TAA Cuts at: 2602 2602 Size: 5032 PstI C_TGCA'G Cuts at: 830 2384 830 Size: 1554 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5032 SacI G_AGCT'C Cuts at: 2123 2123 Size: 5032 SalI G'TCGA_C Cuts at: 1936 2374 1936 Size: 438 4594 SbfI CC_TGCA'GG Cuts at: 2384 2384 Size: 5032 ScaI AGT'ACT Cuts at: 3280 4992 3280 Size: 1712 3320 SfcI C'TryA_G Cuts at: 826 1045 2380 3515 4196 4387 826 Size: 219 1335 1135 681 191 1471 Fragments arranged by size: 1471 1335 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5032 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5032 SmaI CCC'GGG Cuts at: 1872 2359 1872 Size: 487 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2095 2241 3136 4037 4299 820 Size: 197 926 152 146 895 901 262 1553 Fragments arranged by size: 1553 926 901 895 262 197 152 146 SnaBI TAC'GTA Cuts at: 493 2823 493 Size: 2330 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5032 SphI G_CATG'C Cuts at: 1132 2390 1132 Size: 1258 3774 SspI AAT'ATT Cuts at: 5 52 2956 5 Size: 47 2904 2081 Fragments arranged by size: 2904 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5032 StyI C'CwwG_G Cuts at: 513 1065 2065 2471 513 Size: 552 1000 406 3074 Fragments arranged by size: 3074 1000 552 406 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3059 3076 3229 3414 3859 325 Size: 1255 1479 17 153 185 445 1498 Fragments arranged by size: 1498 1479 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3278 4990 96 Size: 274 80 33 51 1443 1301 1712 138 Fragments arranged by size: 1712 1443 1301 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4992 5024 4992 Size: 32 5000 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5032 XbaI T'CTAG_A Cuts at: 2368 2368 Size: 5032 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5032 XmaI C'CCGG_G Cuts at: 1870 2357 1870 Size: 487 4545 XmnI GAAnn'nnTTC Cuts at: 2290 3161 2290 Size: 871 4161 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2840 276 Size: 53 83 186 936 1306 2468 Fragments arranged by size: 2468 1306 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI