################################## # NO CUT ENZYMES ( 183 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FokI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI TfiI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccII 31 CGCG CG^CG AcsI 251 AAATTT [GA]^AATT[CT] AfaI 279 GTAC GT^AC AlwI 261 GGATC GGATC(4/5) AlwNI 127 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 251 AAATTT [GA]^AATT[CT] AspHI 3 GAGCTC G[AT]GC[AT]^C BanII 3 GAGCTC G[GA]GC[CT]^C BbsI 141 GAAGAC GAAGAC(2/6) BclI 188 TGATCA T^GATCA BmyI 3 GAGCTC G[AGT]GC[ACT]^C BpmI 181 CTGGAG CTGGAG(16/14) BpuAI 141 GAAGAC GAAGAC(2/6) BsiHKAI 3 GAGCTC G[AT]GC[AT]^C BsiYI 8 CCGCAGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 8 CCGCAGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 3 GAGCTC G[AGT]GC[ACT]^C Bsp1407I 278 TGTACA T^GTACA BspMI 122 ACCTGC ACCTGC(4/8) BsrGI 278 TGTACA T^GTACA BsrI 76 CCAGT ACTGG(1/-1) BstAPI 267 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 31 CGCG CG^CG BstYI 261 GGATCT [GA]^GATC[CT] Cac8I 267 GCAAGC GC[ACGT]^[ACGT]GC CfoI 32 GCGC GCG^C FbaI 188 TGATCA T^GATCA HaeIII 195 GGCC GG^CC HapII 197 CCGG C^CGG HhaI 32 GCGC GCG^C HinP1I 32 GCGC G^CGC HpaII 197 CCGG C^CGG MflI 261 GGATCT [GA]^GATC[CT] MseI 293 TTAA T^TAA MspI 197 CCGG C^CGG MvnI 31 CGCG CG^CG PstI 124 CTGCAG CTGCA^G RsaI 279 GTAC GT^AC SacI 3 GAGCTC GAGCT^C SfaNI 48 GATGC GCATC(5/9) SfcI 124 CTGCAG C^T[GA][CT]AG SspBI 278 TGTACA T^GTACA Tru9I 293 TTAA T^TAA Tsp509I 252 AATT ^AATT XhoII 261 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BbvI 10 GCAGC GCAGC(8/12) 66 GCTGC BsaJI 152 CCCAGG C^C[ACGT][ACGT]GG 163 CCCAGG DpnII 189 GATC ^GATC 262 GATC DraII 16 AGGTCCC [GA]G^G[ACGT]CC[CT] 119 GGGACCT EcoO109I 16 AGGTCCC [GA]G^G[ACGT]CC[CT] 119 GGGACCT Fnu4HI 10 GCAGC GC^[ACGT]GC 66 GCTGC ItaI 10 GCAGC GC^[ACGT]GC 66 GCTGC MboI 189 GATC ^GATC 262 GATC NdeII 189 GATC ^GATC 262 GATC PpuMI 16 AGGTCCC [GA]G^G[AT]CC[CT] 119 GGGACCT Sau3AI 189 GATC ^GATC 262 GATC TseI 10 GCAGC G^C[AT]GC 66 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AciI AluI AvaII BsmFI BssKI BstNI Cfr13I EcoRII FauI HphI MboII MnlI MvaI MwoI NlaIV Sau96I ScrFI TspRI