(Circular) (Six-base) MAPSORT of: pFN21AE3014.seq Check: 6713 from: 1 to: 5017 pFN21AE3014 5017 bp With 182 enzymes: SgfI * March 28, 2012 12:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2827 278 Size: 53 83 186 936 1291 2468 Fragments arranged by size: 2468 1291 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2360 1937 Size: 423 4594 Acc65I G'GTAC_C Cuts at: 2338 2338 Size: 5017 AclI AA'CG_TT Cuts at: 1563 3144 3517 1563 Size: 1581 373 3063 Fragments arranged by size: 3063 1581 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2127 2133 2261 3080 4464 4732 1632 Size: 495 6 128 819 1384 268 1917 Fragments arranged by size: 1917 1384 819 495 268 128 6 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4820 AflIII A'CryG_T Cuts at: 1203 3916 4893 1203 Size: 2713 977 1327 Fragments arranged by size: 2713 1327 977 AgeI A'CCGG_T Cuts at: 3848 3848 Size: 5017 AhdI GACnn_n'nnGTC Cuts at: 3746 3746 Size: 5017 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5017 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5017 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2042 2074 2042 Size: 32 4985 AlwNI CAG_nnn'CTG Cuts at: 2137 2760 4332 2137 Size: 623 1572 2822 Fragments arranged by size: 2822 1572 623 ApaLI G'TGCA_C Cuts at: 3074 4230 3074 Size: 1156 3861 ApoI r'AATT_y Cuts at: 1493 2256 2326 2552 2704 4813 1493 Size: 763 70 226 152 2109 1697 Fragments arranged by size: 2109 1697 763 226 152 70 AseI AT'TA_AT Cuts at: 160 1032 3571 160 Size: 872 2539 1606 Fragments arranged by size: 2539 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5017 AvaI C'yCGr_G Cuts at: 1870 1943 2342 1870 Size: 73 399 4545 Fragments arranged by size: 4545 399 73 BamHI G'GATC_C Cuts at: 2347 2714 2347 Size: 367 4650 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2338 3793 618 Size: 325 217 627 551 1455 1842 Fragments arranged by size: 1842 1455 627 551 325 217 BanII G_rGCy'C Cuts at: 1391 2012 2336 1391 Size: 621 324 4072 Fragments arranged by size: 4072 621 324 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2153 928 Size: 971 254 3792 Fragments arranged by size: 3792 971 254 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3208 3242 1903 Size: 34 1271 34 3678 Fragments arranged by size: 3678 1271 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2909 4119 2909 Size: 1210 3807 BclI T'GATC_A Cuts at: 1548 2193 1548 Size: 645 4372 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3628 136 Size: 107 122 71 631 605 1956 1525 Fragments arranged by size: 1956 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5012 5012 Size: 5017 BlpI GC'TnA_GC Cuts at: 2434 2434 Size: 5017 Bme1580I G_kGCm'C Cuts at: 1790 3078 4234 1790 Size: 1288 1156 2573 Fragments arranged by size: 2573 1288 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5017 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2748 3706 442 Size: 416 53 83 399 24 1331 958 1753 Fragments arranged by size: 1753 1331 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5017 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 3677 1239 Size: 968 1470 2579 Fragments arranged by size: 2579 1470 968 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3142 4007 4305 3142 Size: 865 298 3854 Fragments arranged by size: 3854 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3680 882 Size: 617 2181 2219 Fragments arranged by size: 2219 2181 617 BsaAI yAC'GTr Cuts at: 493 1204 2808 493 Size: 711 1604 2702 Fragments arranged by size: 2702 1604 711 BsaBI GATnn'nnATC Cuts at: 5011 5011 Size: 5017 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2824 3206 3841 275 Size: 53 83 186 936 1291 382 635 1451 Fragments arranged by size: 1451 1291 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3450 3848 4122 4269 4680 3450 Size: 398 274 147 411 3787 Fragments arranged by size: 3787 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2042 2072 1178 Size: 30 165 30 639 30 4123 Fragments arranged by size: 4123 639 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5017 BseYI C'CCAG_C Cuts at: 4220 4716 4220 Size: 496 4521 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4444 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2467 3228 4256 1540 Size: 275 186 466 761 1028 2301 Fragments arranged by size: 2301 1028 761 466 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 3078 3163 4234 2012 Size: 1066 85 1071 2795 Fragments arranged by size: 2795 1071 1066 85 BsmI GAATG_Cn' Cuts at: 2528 2621 2528 Size: 93 4924 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2336 3078 3163 4234 1391 Size: 399 222 324 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 324 222 85 BspEI T'CCGG_A Cuts at: 4680 4680 Size: 5017 BspHI T'CATG_A Cuts at: 2906 4636 2906 Size: 1730 3287 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2136 2372 844 Size: 1292 236 3489 Fragments arranged by size: 3489 1292 236 BsrBI CCG'CTC Cuts at: 1394 1823 2904 1394 Size: 429 1081 3507 Fragments arranged by size: 3507 1081 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3512 3686 65 Size: 1183 2264 174 1396 Fragments arranged by size: 2264 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3661 3848 1700 Size: 110 1851 187 2869 Fragments arranged by size: 2869 1851 187 110 BsrGI T'GTAC_A Cuts at: 96 2283 96 Size: 2187 2830 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5017 BssSI C'ACGA_G Cuts at: 3077 4089 3077 Size: 1012 4005 BstAPI GCAn_nnn'nTGC Cuts at: 794 2278 794 Size: 1484 3533 BstBI TT'CG_AA Cuts at: 3821 3821 Size: 5017 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5017 BstYI r'GATC_y Cuts at: 1972 2266 2347 2714 3099 3116 4568 4654 4666 Size: 294 81 367 385 17 1452 86 12 Cuts at: 4666 5012 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 294 86 81 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4465 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4278 Fragments arranged by size: 4278 683 56 BtsI GCAGTG_nn' Cuts at: 2138 2209 2217 2533 3327 3347 2138 Size: 71 8 316 794 20 3808 Fragments arranged by size: 3808 794 316 71 20 8 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5017 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4982 DraI TTT'AAA Cuts at: 2321 2321 Size: 5017 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4024 809 Size: 507 2708 1802 Fragments arranged by size: 2708 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2464 3353 4982 Size: 54 1425 50 275 18 634 889 1629 Cuts at: 4982 8 Size: 43 Fragments arranged by size: 1629 1425 889 634 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2464 1537 Size: 275 652 4090 Fragments arranged by size: 4090 652 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2947 1353 Size: 1594 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3604 3978 4124 387 Size: 1064 138 2015 374 146 1280 Fragments arranged by size: 2015 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2010 2010 Size: 5017 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2127 2133 2207 2261 3080 3677 4464 Size: 393 495 6 74 54 819 597 787 Cuts at: 4464 4732 1239 Size: 268 1524 Fragments arranged by size: 1524 819 787 597 495 393 268 74 54 6 EcoO109I rG'GnC_Cy Cuts at: 2022 2125 2022 Size: 103 4914 EcoRI G'AATT_C Cuts at: 2326 2704 2326 Size: 378 4639 FspI TGC'GCA Cuts at: 3523 3523 Size: 5017 HaeII r_GCGC'y Cuts at: 1678 4164 1678 Size: 2486 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3658 3690 3732 3764 1373 Size: 32 135 32 2086 32 42 32 2626 Fragments arranged by size: 2626 2086 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2361 2607 669 Size: 988 281 423 246 3079 Fragments arranged by size: 3079 988 423 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5017 HpaI GTT'AAC Cuts at: 2607 2607 Size: 5017 KpnI G_GTAC'C Cuts at: 2342 2342 Size: 5017 MfeI C'AATT_G Cuts at: 2616 2616 Size: 5017 MluI A'CGCG_T Cuts at: 4893 4893 Size: 5017 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4131 4315 1305 Size: 2826 184 2007 Fragments arranged by size: 2826 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3195 Fragments arranged by size: 3195 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2975 3334 3493 518 Size: 648 1809 359 159 2042 Fragments arranged by size: 2042 1809 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2433 3110 4258 4503 1606 Size: 827 677 1148 245 2120 Fragments arranged by size: 2120 1148 827 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4465 NdeI CA'TA_TG Cuts at: 387 387 Size: 5017 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5017 NotI GC'GGCC_GC Cuts at: 2464 2464 Size: 5017 NspI r_CATG'y Cuts at: 1132 1229 2375 3920 1132 Size: 97 1146 1545 2229 Fragments arranged by size: 2229 1545 1146 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5017 PciI A'CATG_T Cuts at: 3916 3916 Size: 5017 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5017 PmeI GTTT'AAAC Cuts at: 2321 2321 Size: 5017 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2042 2074 3086 3118 4599 4631 2042 Size: 32 1012 32 1481 32 2428 Fragments arranged by size: 2428 1481 1012 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2022 2125 2022 Size: 103 4914 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5017 PsiI TTA'TAA Cuts at: 2587 2587 Size: 5017 PstI C_TGCA'G Cuts at: 830 2133 2369 830 Size: 1303 236 3478 Fragments arranged by size: 3478 1303 236 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5017 SacI G_AGCT'C Cuts at: 2012 2012 Size: 5017 SalI G'TCGA_C Cuts at: 1936 2359 1936 Size: 423 4594 SbfI CC_TGCA'GG Cuts at: 2369 2369 Size: 5017 ScaI AGT'ACT Cuts at: 3265 4977 3265 Size: 1712 3305 SfcI C'TryA_G Cuts at: 826 1045 2129 2365 3500 4181 4372 826 Size: 219 1084 236 1135 681 191 1471 Fragments arranged by size: 1471 1135 1084 681 236 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5017 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5017 SmaI CCC'GGG Cuts at: 1872 2344 1872 Size: 472 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3121 4022 4284 820 Size: 197 926 1178 901 262 1553 Fragments arranged by size: 1553 1178 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2808 493 Size: 2315 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5017 SphI G_CATG'C Cuts at: 1132 2375 1132 Size: 1243 3774 SspI AAT'ATT Cuts at: 5 52 2941 5 Size: 47 2889 2081 Fragments arranged by size: 2889 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5017 StyI C'CwwG_G Cuts at: 513 1065 2456 513 Size: 552 1391 3074 Fragments arranged by size: 3074 1391 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3044 3061 3214 3399 3844 325 Size: 1255 1464 17 153 185 445 1498 Fragments arranged by size: 1498 1464 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2283 3263 4975 Size: 274 80 33 51 1443 306 980 1712 Cuts at: 4975 96 Size: 138 Fragments arranged by size: 1712 1443 980 306 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2117 2149 4977 5009 2117 Size: 32 2828 32 2125 Fragments arranged by size: 2828 2125 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5017 XbaI T'CTAG_A Cuts at: 2353 2353 Size: 5017 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5017 XmaI C'CCGG_G Cuts at: 1870 2342 1870 Size: 472 4545 XmnI GAAnn'nnTTC Cuts at: 3146 3146 Size: 5017 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2825 276 Size: 53 83 186 936 1291 2468 Fragments arranged by size: 2468 1291 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI