################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII BalI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AciI 414 CCGC CCGC(-3/-1) AcsI 860 GAATTT [GA]^AATT[CT] ApoI 860 GAATTT [GA]^AATT[CT] AspHI 779 GAGCAC G[AT]GC[AT]^C AspI 93 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 855 CCTAGG C^CTAGG BamHI 491 GGATCC G^GATCC BbsI 789 GAAGAC GAAGAC(2/6) BclI 401 TGATCA T^GATCA BlnI 855 CCTAGG C^CTAGG BmyI 779 GAGCAC G[AGT]GC[ACT]^C BpmI 326 CTGGAG CTGGAG(16/14) BpuAI 789 GAAGAC GAAGAC(2/6) BsiHKAI 779 GAGCAC G[AT]GC[AT]^C BsmAI 268 GAGAC GTCTC(1/5) Bsp1286I 779 GAGCAC G[AGT]GC[ACT]^C BspHI 389 TCATGA T^CATGA BsrBI 414 CCGCTC CCGCTC(-3/-3) BsrDI 689 GCAATG GCAATG(2/0) BssSI 429 CACGAG CACGAG(-5/-1) BstAPI 4 GCAAACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 257 CCAACCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 939 TTTAAA TTT^AAA DsaI 473 CCATGG C^C[GA][CT]GG EarI 655 CTCTTC CTCTTC(1/4) EcoNI 723 CCTTCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 401 TGATCA T^GATCA HindIII 361 AAGCTT A^AGCTT Ksp632I 655 CTCTTC CTCTTC(1/4) MspA1I 648 CAGCTG C[AC]G^C[GT]G NcoI 473 CCATGG C^CATGG PflFI 93 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 286 CCACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 714 GACACGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 597 CTGCAG CTGCA^G PvuII 648 CAGCTG CAG^CTG RcaI 389 TCATGA T^CATGA SfcI 597 CTGCAG C^T[GA][CT]AG SmlI 219 CTCAAG C^T[CT][GA]AG Tsp45I 115 GTGAC ^GT[GC]AC Tsp509I 861 AATT ^AATT Tth111I 93 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 286 CCACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BbvI 231 GCTGC GCAGC(8/12) 650 GCTGC BsrI 288 ACTGG ACTGG(1/-1) 907 CCAGT BstYI 107 AGATCC [GA]^GATC[CT] 491 GGATCC EcoT14I 473 CCATGG C^C[AT][AT]GG 855 CCTAGG Fnu4HI 231 GCTGC GC^[ACGT]GC 650 GCTGC ItaI 231 GCTGC GC^[ACGT]GC 650 GCTGC MflI 107 AGATCC [GA]^GATC[CT] 491 GGATCC MslI 390 CATGATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 608 CACAATGGTG MwoI 4 GCAAACAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 407 GCCTGGACCGC PpuMI 719 GGGTCCT [GA]G^G[AT]CC[CT] 731 AGGACCC StyI 473 CCATGG C^C[AT][AT]GG 855 CCTAGG TseI 231 GCTGC G^C[AT]GC 650 GCTGC XcmI 315 CCAATGGTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 473 CCATGGTTGGCCTGG XhoII 107 AGATCC [GA]^GATC[CT] 491 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AluI AlwI AvaII BfaI BsaJI BsiYI BslI BssKI BstF5I BstNI Cfr13I DdeI DpnII DraII EcoO109I EcoRII FokI HaeIII HinfI HphI MaeI MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TspRI