################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccI AccIII AcyI AflII AflIII AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BlnI BsaAI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BsrDI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HpaI HphI KasI KpnI Ksp632I KspI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI StuI SwaI TfiI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AclI 1745 AACGTT AA^CGTT AgeI 56 ACCGGT A^CCGGT Alw44I 551 GTGCAC G^TGCAC ApaLI 551 GTGCAC G^TGCAC AspHI 551 GTGCAC G[AT]GC[AT]^C AvaI 1003 CCCGAG C^[CT]CG[GA]G BanII 1952 GGGCTC G[GA]GC[CT]^C BcnI 1758 CCGGG CC^[GC]GG BfaI 787 CTAG C^TAG BglI 65 GCCAGAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 383 GCTTAGC GC^T[ACGT]AGC Bpu1102I 383 GCTTAGC GC^T[ACGT]AGC BsaBI 1096 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 56 ACCGGT [AT]^CCGG[AT] BsiHKAI 551 GTGCAC G[AT]GC[AT]^C BsiYI 1876 CCAAGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 1876 CCAAGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 1536 GTCTC GTCTC(1/5) BsoBI 1003 CCCGAG C^[CT]CG[GA]G Bsp1407I 944 TGTACA T^GTACA BsrBI 6 GAGCGG CCGCTC(-3/-3) BsrFI 56 ACCGGT [GA]^CCGG[CT] BsrGI 944 TGTACA T^GTACA BssHII 84 GCGCGC G^CGCGC BstAPI 457 GCAGTTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1720 CCAGAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 383 GCTTAGC GC^T[ACGT]AGC Cfr10I 56 ACCGGT [GA]^CCGG[CT] DraII 1801 GGGACCC [GA]G^G[ACGT]CC[CT] DrdI 826 GACCAAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1133 CCATGG C^C[GA][CT]GG EcoO109I 1801 GGGACCC [GA]G^G[ACGT]CC[CT] FauI 17 GCGGG CCCGC(4/6) HincII 1622 GTCAAC GT[CT]^[GA]AC HindII 1622 GTCAAC GT[CT]^[GA]AC HinfI 841 GACTC G^A[ACGT]TC MaeI 787 CTAG C^TAG MamI 1096 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 477 CAATTG C^AATTG MslI 213 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 477 CAATTG C^AATTG NciI 1758 CCGGG CC^[GC]GG NcoI 1133 CCATGG C^CATGG PflMI 1876 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 56 ACCGGT A^CCGGT PleI 841 GACTC GAGTC(4/5) PpuMI 1801 GGGACCC [GA]G^G[AT]CC[CT] Psp1406I 1745 AACGTT AA^CGTT SbfI 1945 CCTGCAGG CCTGCA^GG Sse8387I 1945 CCTGCAGG CCTGCA^GG SspBI 944 TGTACA T^GTACA TaqI 720 TCGA T^CGA Tsp45I 1403 GTGAC ^GT[GC]AC TthHB8I 720 TCGA T^CGA Van91I 1876 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 83 CGCG CG^CG 85 CGCG AlwNI 29 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG 1898 CAGTATCTG AvaII 825 GGACC G^G[AT]CC 1802 GGACC BalI 63 TGGCCA TGG^CCA 674 TGGCCA BglII 399 AGATCT A^GATCT 609 AGATCT BmyI 551 GTGCAC G[AGT]GC[ACT]^C 1952 GGGCTC BpmI 1955 CTCCAG CTGGAG(16/14) 1613 CTGGAG BsmI 285 GAATGC GAATGC(1/-1) 379 GAATGC Bsp1286I 551 GTGCAC G[AGT]GC[ACT]^C 1952 GGGCTC BspMI 33 ACCTGC ACCTGC(4/8) 1944 ACCTGC BstF5I 1102 CATCC GGATG(2/0) 1839 GGATG BstUI 83 CGCG CG^CG 85 CGCG EaeI 63 TGGCCA [CT]^GGCC[GA] 674 TGGCCA EcoT14I 564 CCAAGG C^C[AT][AT]GG 1133 CCATGG FokI 1102 CATCC GGATG(9/13) 1839 GGATG HapII 57 CCGG C^CGG 1758 CCGG HpaII 57 CCGG C^CGG 1758 CCGG MaeII 131 ACGT A^CGT 1746 ACGT MluNI 63 TGGCCA TGG^CCA 674 TGGCCA MscI 63 TGGCCA TGG^CCA 674 TGGCCA MspI 57 CCGG C^CGG 1758 CCGG MvnI 83 CGCG CG^CG 85 CGCG NspI 547 GCATGT [GA]CATG^[CT] 1174 GCATGT SmlI 91 CTCAAG C^T[CT][GA]AG 1154 CTCAAG SspI 1162 AATATT AAT^ATT 1182 AATATT StyI 564 CCAAGG C^C[AT][AT]GG 1133 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BbsI BbvI BpuAI BsaJI BseRI BsmFI BsrI BssKI BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HhaI HinP1I HindIII ItaI MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI Tru9I TseI Tsp509I TspRI XhoII