################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## Acc65I AccI AclI Aor51HI AscI Asp700I Asp718I AviII AvrII BbeI BbvCI BcgI BlnI BsaBI BsiEI BsiWI BspDI BspHI BsrBI BssHII BssSI Bst1107I BstAPI BstZ17I ClaI DrdI EagI EclXI Eco47III Eco52I EcoNI FseI FspI HgaI HpaI KasI KpnI KspI MamI MfeI MluI MspA1I MunI NarI NcoI NdeI NheI NotI PacI PaeR7I PmeI PshAI Psp1406I PvuI PvuII RcaI SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI SpeI Sse8387I SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 3259 GACGTC GACGT^C AccII 3062 CGCG CG^CG AccIII 3919 TCCGGA T^CCGGA AcyI 3259 GACGTC G[GA]^CG[CT]C AflII 1885 CTTAAG C^TTAAG AgeI 4006 ACCGGT A^CCGGT Alw44I 1710 GTGCAC G^TGCAC ApaI 3377 GGGCCC GGGCC^C ApaLI 1710 GTGCAC G^TGCAC AseI 610 ATTAAT AT^TAAT AsnI 610 ATTAAT AT^TAAT AspI 1915 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2535 TGGCCA TGG^CCA BamHI 2418 GGATCC G^GATCC BbrPI 1636 CACGTG CAC^GTG BfrI 1885 CTTAAG C^TTAAG BglI 2721 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 1636 CACGTG [CT]AC^GT[GA] BsaHI 3259 GACGTC G[GA]^CG[CT]C BseAI 3919 TCCGGA T^CCGGA BsmBI 689 CGTCTC CGTCTC(1/5) BspEI 3919 TCCGGA T^CCGGA BspLU11I 1330 ACATGT A^CATGT BspMI 2206 ACCTGC ACCTGC(4/8) BsrDI 1957 CATTGC GCAATG(2/0) BstBI 792 TTCGAA TT^CGAA BstUI 3062 CGCG CG^CG BstXI 2408 CCATCGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CpoI 2359 CGGACCG CG^G[AT]CCG EcoT22I 1892 ATGCAT ATGCA^T HaeII 2882 GGCGCT [GA]GCGC^[CT] Hin1I 3259 GACGTC G[GA]^CG[CT]C MluNI 2535 TGGCCA TGG^CCA MroI 3919 TCCGGA T^CCGGA MscI 2535 TGGCCA TGG^CCA MvnI 3062 CGCG CG^CG NaeI 3692 GCCGGC GCC^GGC NgoMIV 3692 GCCGGC G^CCGGC NruI 3061 TCGCGA TCG^CGA NsiI 1892 ATGCAT ATGCA^T NspV 792 TTCGAA TT^CGAA PflFI 1915 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 4006 ACCGGT A^CCGGT PmaCI 1636 CACGTG CAC^GTG PmlI 1636 CACGTG CAC^GTG PshBI 610 ATTAAT AT^TAAT RsrII 2359 CGGACCG CG^G[AT]CCG SapI 3799 GAAGAGC GCTCTTC(1/4) SfuI 792 TTCGAA TT^CGAA SphI 2215 GCATGC GCATG^C SspI 3291 AATATT AAT^ATT StuI 2196 AGGCCT AGG^CCT Tth111I 1915 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AflIII 473 ACGTGT A^C[GA][CT]GT 1330 ACATGT AhdI 1641 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3259 GACGTCTGGTC AlwNI 1765 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG 3664 CAGTATCTG AocI 2781 CCTGAGG CC^T[ACGT]AGG 3299 CCTTAGG AspEI 1641 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3259 GACGTCTGGTC BciVI 1818 GGATAC GTATCC(6/5) 3071 GTATCC BclI 572 TGATCA T^GATCA 1874 TGATCA BsgI 234 GTGCAG GTGCAG(16/14) 920 GTGCAG BsmI 1836 GAATGC GAATGC(1/-1) 1890 GAATGC BstEII 1066 GGTTACC G^GT[ACGT]ACC 2677 GGTCACC BstPI 1066 GGTTACC G^GT[ACGT]ACC 2677 GGTCACC Bsu36I 2781 CCTGAGG CC^T[ACGT]AGG 3299 CCTTAGG CfoI 1397 GCGC GCG^C 2883 GCGC DraI 2101 TTTAAA TTT^AAA 3978 TTTAAA DraIII 479 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG 2624 CACACAGTG DsaI 1025 CCACGG C^C[GA][CT]GG 2523 CCGTGG Eam1105I 1641 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3259 GACGTCTGGTC Eco81I 2781 CCTGAGG CC^T[ACGT]AGG 3299 CCTTAGG EcoO65I 1066 GGTTACC G^GT[ACGT]ACC 2677 GGTCACC EcoRI 2482 GAATTC G^AATTC 2749 GAATTC FbaI 572 TGATCA T^GATCA 1874 TGATCA HhaI 1397 GCGC GCG^C 2883 GCGC HinP1I 1397 GCGC G^CGC 2883 GCGC HincII 1597 GTCAAC GT[CT]^[GA]AC 2695 GTCAAC HindII 1597 GTCAAC GT[CT]^[GA]AC 2695 GTCAAC HindIII 721 AAGCTT A^AGCTT 1753 AAGCTT PpuMI 1647 GGGTCCT [GA]G^G[AT]CC[CT] 3080 GGGACCT PstI 1715 CTGCAG CTGCA^G 3670 CTGCAG SmaI 50 CCCGGG CCC^GGG 3149 CCCGGG XmaCI 50 CCCGGG C^CCGGG 3149 CCCGGG XmaI 50 CCCGGG C^CCGGG 3149 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BsrFI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII