(Linear) (Six-base) MAPSORT of: KIBB5621.seq Check: 1825 from: 1 to: 4068 KIBB5621.orf With 182 enzymes: SgfI * March 22, 2012 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2215 4068 Size: 2215 1853 AatII G_ACGT'C Cuts at: 0 3263 4068 Size: 3263 805 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2410 2521 2996 3908 4068 Size: 2410 111 475 912 160 Fragments arranged by size: 2410 912 475 160 111 AflII C'TTAA_G Cuts at: 0 1885 4068 Size: 1885 2183 AflIII A'CryG_T Cuts at: 0 473 1330 4068 Size: 473 857 2738 Fragments arranged by size: 2738 857 473 AgeI A'CCGG_T Cuts at: 0 4006 4068 Size: 4006 62 AhdI GACnn_n'nnGTC Cuts at: 0 1646 3264 4068 Size: 1646 1618 804 Fragments arranged by size: 1646 1618 804 AleI CACnn'nnGTG Cuts at: 0 2314 3949 4068 Size: 2314 1635 119 Fragments arranged by size: 2314 1635 119 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 31 3515 4068 Size: 31 3484 553 Fragments arranged by size: 3484 553 31 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2645 2677 4068 Size: 2645 32 1391 Fragments arranged by size: 2645 1391 32 AlwNI CAG_nnn'CTG Cuts at: 0 1770 3669 4068 Size: 1770 1899 399 Fragments arranged by size: 1899 1770 399 ApaI G_GGCC'C Cuts at: 0 3381 4068 Size: 3381 687 ApaLI G'TGCA_C Cuts at: 0 1710 4068 Size: 1710 2358 ApoI r'AATT_y Cuts at: 0 527 859 2482 2749 2758 2800 3340 3631 Size: 527 332 1623 267 9 42 540 291 Cuts at: 3631 4068 Size: 437 Fragments arranged by size: 1623 540 527 437 332 291 267 42 9 AseI AT'TA_AT Cuts at: 0 611 4068 Size: 611 3457 AvaI C'yCGr_G Cuts at: 0 50 451 3149 4038 4068 Size: 50 401 2698 889 30 Fragments arranged by size: 2698 889 401 50 30 BamHI G'GATC_C Cuts at: 0 2418 4068 Size: 2418 1650 BanI G'GyrC_C Cuts at: 0 1312 1995 2266 2527 2707 3855 4068 Size: 1312 683 271 261 180 1148 213 Fragments arranged by size: 1312 1148 683 271 261 213 180 BanII G_rGCy'C Cuts at: 0 1409 2643 2914 3381 3440 4057 4068 Size: 1409 1234 271 467 59 617 11 Fragments arranged by size: 1409 1234 617 467 271 59 11 BbsI GAAGACnn'nnnn_ Cuts at: 0 585 1948 2132 2398 3824 4068 Size: 585 1363 184 266 1426 244 Fragments arranged by size: 1426 1363 585 266 244 184 BciVI GTATCCnnnnn_n' Cuts at: 0 1812 3082 4068 Size: 1812 1270 986 Fragments arranged by size: 1812 1270 986 BclI T'GATC_A Cuts at: 0 572 1874 4068 Size: 572 1302 2194 Fragments arranged by size: 2194 1302 572 BfrBI ATG'CAT Cuts at: 0 1894 4068 Size: 1894 2174 BglI GCCn_nnn'nGGC Cuts at: 0 2727 4068 Size: 2727 1341 BglII A'GATC_T Cuts at: 0 364 1723 2979 4068 Size: 364 1359 1256 1089 Fragments arranged by size: 1359 1256 1089 364 BlpI GC'TnA_GC Cuts at: 0 100 2913 3503 4068 Size: 100 2813 590 565 Fragments arranged by size: 2813 590 565 100 Bme1580I G_kGCm'C Cuts at: 0 1714 2271 2829 3381 3860 4068 Size: 1714 557 558 552 479 208 Fragments arranged by size: 1714 558 557 552 479 208 BmrI ACTGGGnnnn_n' Cuts at: 0 786 831 3434 4068 Size: 786 45 2603 634 Fragments arranged by size: 2603 786 634 45 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 707 2749 4068 Size: 707 2042 1319 Fragments arranged by size: 2042 1319 707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 314 2038 3942 4012 4068 Size: 314 1724 1904 70 56 Fragments arranged by size: 1904 1724 314 70 56 Bpu10I CC'TnA_GC Cuts at: 0 3038 4068 Size: 3038 1030 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1132 1916 2641 4068 Size: 1132 784 725 1427 Fragments arranged by size: 1427 1132 784 725 BsaI GGTCTCn'nnnn_ Cuts at: 0 40 818 1242 2107 2486 3441 4068 Size: 40 778 424 865 379 955 627 Fragments arranged by size: 955 865 778 627 424 379 40 BsaAI yAC'GTr Cuts at: 0 1638 4068 Size: 1638 2430 BsaHI Gr'CG_yC Cuts at: 0 3260 4068 Size: 3260 808 BsaWI w'CCGG_w Cuts at: 0 2156 3919 4006 4068 Size: 2156 1763 87 62 Fragments arranged by size: 2156 1763 87 62 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1378 1408 1607 1637 1684 1714 1904 1934 Size: 1378 30 199 30 47 30 190 30 Cuts at: 1934 2926 2956 4068 Size: 992 30 1112 Fragments arranged by size: 1378 1112 992 199 190 47 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1628 2799 2950 3622 3625 4049 4068 Size: 1628 1171 151 672 3 424 19 Fragments arranged by size: 1628 1171 672 424 151 19 3 BseYI C'CCAG_C Cuts at: 0 562 1408 3859 4068 Size: 562 846 2451 209 Fragments arranged by size: 2451 846 562 209 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 255 941 4068 Size: 255 686 3127 Fragments arranged by size: 3127 686 255 BsiHKAI G_wGCw'C Cuts at: 0 871 1714 2643 2914 4057 4068 Size: 871 843 929 271 1143 11 Fragments arranged by size: 1143 929 871 843 271 11 BsmI GAATG_Cn' Cuts at: 0 1842 1896 4068 Size: 1842 54 2172 Fragments arranged by size: 2172 1842 54 BsmBI CGTCTCn'nnnn_ Cuts at: 0 695 4068 Size: 695 3373 Bsp1286I G_dGCh'C Cuts at: 0 871 1409 1714 2271 2643 2829 2914 3381 Size: 871 538 305 557 372 186 85 467 Cuts at: 3381 3440 3860 4057 4068 Size: 59 420 197 11 Fragments arranged by size: 871 557 538 467 420 372 305 197 186 85 59 11 BspEI T'CCGG_A Cuts at: 0 3919 4068 Size: 3919 149 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2215 4068 Size: 2215 1853 BsrDI GCAATG_nn' Cuts at: 0 1956 4068 Size: 1956 2112 BsrFI r'CCGG_y Cuts at: 0 2495 3692 4006 4068 Size: 2495 1197 314 62 Fragments arranged by size: 2495 1197 314 62 BsrGI T'GTAC_A Cuts at: 0 407 638 736 911 1580 3242 4068 Size: 407 231 98 175 669 1662 826 Fragments arranged by size: 1662 826 669 407 231 175 98 BstBI TT'CG_AA Cuts at: 0 793 4068 Size: 793 3275 BstEII G'GTnAC_C Cuts at: 0 1066 2677 4068 Size: 1066 1611 1391 Fragments arranged by size: 1611 1391 1066 BstXI CCAn_nnnn'nTGG Cuts at: 0 2415 4068 Size: 2415 1653 BstYI r'GATC_y Cuts at: 0 364 1723 2418 2660 2871 2979 3165 4068 Size: 364 1359 695 242 211 108 186 903 Fragments arranged by size: 1359 903 695 364 242 211 186 108 Bsu36I CC'TnA_GG Cuts at: 0 2782 3300 4068 Size: 2782 518 768 Fragments arranged by size: 2782 768 518 BtgI C'CryG_G Cuts at: 0 1025 2523 4068 Size: 1025 1498 1545 Fragments arranged by size: 1545 1498 1025 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 254 3044 4068 Size: 254 2790 1024 Fragments arranged by size: 2790 1024 254 BtsI GCAGTG_nn' Cuts at: 0 213 1712 2226 4068 Size: 213 1499 514 1842 Fragments arranged by size: 1842 1499 514 213 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1357 1392 1763 1798 2849 2884 4068 Size: 1357 35 371 35 1051 35 1184 Fragments arranged by size: 1357 1184 1051 371 35 35 35 DraI TTT'AAA Cuts at: 0 2103 3980 4068 Size: 2103 1877 88 Fragments arranged by size: 2103 1877 88 DraIII CAC_nnn'GTG Cuts at: 0 484 2629 4068 Size: 484 2145 1439 Fragments arranged by size: 2145 1439 484 EaeI y'GGCC_r Cuts at: 0 20 2520 2535 4068 Size: 20 2500 15 1533 Fragments arranged by size: 2500 1533 20 15 EarI CTCTTCn'nnn_ Cuts at: 0 149 253 1385 2957 3554 3794 4068 Size: 149 104 1132 1572 597 240 274 Fragments arranged by size: 1572 1132 597 274 240 149 104 EcoICRI GAG'CTC Cuts at: 0 2641 2912 4055 4068 Size: 2641 271 1143 13 Fragments arranged by size: 2641 1143 271 13 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 314 2038 2410 2521 2996 3908 3942 4012 Size: 314 1724 372 111 475 912 34 70 Cuts at: 4012 4068 Size: 56 Fragments arranged by size: 1724 912 475 372 314 111 70 56 34 EcoO109I rG'GnC_Cy Cuts at: 0 1648 3081 3377 3378 4068 Size: 1648 1433 296 1 690 Fragments arranged by size: 1648 1433 690 296 1 EcoRI G'AATT_C Cuts at: 0 2482 2749 4068 Size: 2482 267 1319 Fragments arranged by size: 2482 1319 267 EcoRV GAT'ATC Cuts at: 0 3539 3723 3925 4068 Size: 3539 184 202 143 Fragments arranged by size: 3539 202 184 143 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 136 567 744 2301 3991 4068 Size: 136 431 177 1557 1690 77 Fragments arranged by size: 1690 1557 431 177 136 77 HaeII r_GCGC'y Cuts at: 0 2886 4068 Size: 2886 1182 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 316 348 349 381 432 464 1632 1664 Size: 316 32 1 32 51 32 1168 32 Cuts at: 1664 1684 1716 1904 1936 2926 2958 3250 3282 Size: 20 32 188 32 990 32 292 32 Cuts at: 3282 4068 Size: 786 Fragments arranged by size: 1168 990 786 316 292 188 51 32 32 32 32 32 32 32 32 20 1 HincII GTy'rAC Cuts at: 0 1599 2697 4068 Size: 1599 1098 1371 Fragments arranged by size: 1599 1371 1098 HindIII A'AGCT_T Cuts at: 0 721 1753 4068 Size: 721 1032 2315 Fragments arranged by size: 2315 1032 721 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 693 1364 2835 4068 Size: 693 671 1471 1233 Fragments arranged by size: 1471 1233 693 671 MscI TGG'CCA Cuts at: 0 2537 4068 Size: 2537 1531 MslI CAynn'nnrTG Cuts at: 0 2314 2413 2687 3932 3949 4068 Size: 2314 99 274 1245 17 119 Fragments arranged by size: 2314 1245 274 119 99 17 NaeI GCC'GGC Cuts at: 0 3694 4068 Size: 3694 374 NgoMIV G'CCGG_C Cuts at: 0 3692 4068 Size: 3692 376 NruI TCG'CGA Cuts at: 0 3063 4068 Size: 3063 1005 NsiI A_TGCA'T Cuts at: 0 1896 4068 Size: 1896 2172 NspI r_CATG'y Cuts at: 0 1144 1334 1677 2219 4068 Size: 1144 190 343 542 1849 Fragments arranged by size: 1849 1144 542 343 190 PciI A'CATG_T Cuts at: 0 1330 4068 Size: 1330 2738 PflMI CCAn_nnn'nTGG Cuts at: 0 2477 3319 3844 4068 Size: 2477 842 525 224 Fragments arranged by size: 2477 842 525 224 PmlI CAC'GTG Cuts at: 0 1638 4068 Size: 1638 2430 PpuMI rG'GwC_Cy Cuts at: 0 1648 3081 4068 Size: 1648 1433 987 Fragments arranged by size: 1648 1433 987 PsiI TTA'TAA Cuts at: 0 3161 4068 Size: 3161 907 PspOMI G'GGCC_C Cuts at: 0 3377 4068 Size: 3377 691 PstI C_TGCA'G Cuts at: 0 1719 3674 4068 Size: 1719 1955 394 Fragments arranged by size: 1955 1719 394 RsrII CG'GwC_CG Cuts at: 0 2360 4068 Size: 2360 1708 SacI G_AGCT'C Cuts at: 0 2643 2914 4057 4068 Size: 2643 271 1143 11 Fragments arranged by size: 2643 1143 271 11 SapI GCTCTTCn'nnn_ Cuts at: 0 3794 4068 Size: 3794 274 SfcI C'TryA_G Cuts at: 0 878 1715 3670 4068 Size: 878 837 1955 398 Fragments arranged by size: 1955 878 837 398 SmaI CCC'GGG Cuts at: 0 52 3151 4068 Size: 52 3099 917 Fragments arranged by size: 3099 917 52 SmlI C'TyrA_G Cuts at: 0 1111 1885 1931 2656 4068 Size: 1111 774 46 725 1412 Fragments arranged by size: 1412 1111 774 725 46 SphI G_CATG'C Cuts at: 0 2219 4068 Size: 2219 1849 SspI AAT'ATT Cuts at: 0 3293 4068 Size: 3293 775 StuI AGG'CCT Cuts at: 0 2198 4068 Size: 2198 1870 StyI C'CwwG_G Cuts at: 0 903 1062 1908 3002 4068 Size: 903 159 846 1094 1066 Fragments arranged by size: 1094 1066 903 846 159 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 324 2013 4068 Size: 324 1689 2055 Fragments arranged by size: 2055 1689 324 TatI w'GTAC_w Cuts at: 0 407 638 736 911 1143 1580 3242 3953 Size: 407 231 98 175 232 437 1662 711 Cuts at: 3953 4068 Size: 115 Fragments arranged by size: 1662 711 437 407 232 231 175 115 98 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1378 1410 1607 1639 3083 3115 4068 Size: 1378 32 197 32 1444 32 953 Fragments arranged by size: 1444 1378 953 197 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1918 4068 Size: 1918 2150 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1085 2093 3775 4068 Size: 1085 1008 1682 293 Fragments arranged by size: 1682 1085 1008 293 XmaI C'CCGG_G Cuts at: 0 50 3149 4068 Size: 50 3099 919 Fragments arranged by size: 3099 919 50 ZraI GAC'GTC Cuts at: 0 3261 4068 Size: 3261 807 Enzymes that do cut: AarI AatII AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AvaI BamHI BanI BanII BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BsrGI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII MmeI MscI MslI NaeI NgoMIV NruI NsiI NspI PciI PflMI PmlI PpuMI PsiI PspOMI PstI RsrII SacI SapI SfcI SmaI SmlI SphI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AscI AsiSI AvrII BaeI BbeI BbvCI BcgI BmgBI BmtI BsaBI BsiEI BsiWI BspHI BsrBI BssHII BssSI BstAPI BstZ17I ClaI DrdI EagI EciI EcoNI FseI FspI FspAI HpaI KasI KpnI MfeI MluI MspA1I NarI NcoI NdeI NheI NotI PacI PasI PfoI PmeI PpiI PshAI PspXI PsrI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SwaI XbaI XhoI XmnI