(Circular) (Six-base) MAPSORT of: pFN21AB6281.seq Check: 7010 from: 1 to: 7099 pFN21AB6281 7099 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2620 4909 278 Size: 53 83 186 936 1084 2289 2468 Fragments arranged by size: 2468 2289 1084 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4442 1937 Size: 2505 4594 Acc65I G'GTAC_C Cuts at: 4420 4420 Size: 7099 AclI AA'CG_TT Cuts at: 1563 5226 5599 1563 Size: 3663 373 3063 Fragments arranged by size: 3663 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2972 3959 5162 6546 6814 1632 Size: 1340 987 1203 1384 268 1917 Fragments arranged by size: 1917 1384 1340 1203 987 268 AflII C'TTAA_G Cuts at: 820 1017 2435 2854 820 Size: 197 1418 419 5065 Fragments arranged by size: 5065 1418 419 197 AflIII A'CryG_T Cuts at: 1203 2016 5998 6975 1203 Size: 813 3982 977 1327 Fragments arranged by size: 3982 1327 977 813 AgeI A'CCGG_T Cuts at: 3446 5930 3446 Size: 2484 4615 AhdI GACnn_n'nnGTC Cuts at: 2035 5828 2035 Size: 3793 3306 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7099 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7099 AlwNI CAG_nnn'CTG Cuts at: 4842 6414 4842 Size: 1572 5527 ApaLI G'TGCA_C Cuts at: 4120 5156 6312 4120 Size: 1036 1156 4907 Fragments arranged by size: 4907 1156 1036 ApoI r'AATT_y Cuts at: 1493 2563 3508 3847 4408 4634 4786 6895 1493 Size: 1070 945 339 561 226 152 2109 1697 Fragments arranged by size: 2109 1697 1070 945 561 339 226 152 AseI AT'TA_AT Cuts at: 160 1032 3174 3198 5653 160 Size: 872 2142 24 2455 1606 Fragments arranged by size: 2455 2142 1606 872 24 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7099 AvaI C'yCGr_G Cuts at: 1870 1943 4424 1870 Size: 73 2481 4545 Fragments arranged by size: 4545 2481 73 BamHI G'GATC_C Cuts at: 4429 4796 4429 Size: 367 6732 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2396 2452 2974 4420 5875 Size: 325 217 627 609 56 522 1446 1455 Cuts at: 5875 618 Size: 1842 Fragments arranged by size: 1842 1455 1446 627 609 522 325 217 56 BanII G_rGCy'C Cuts at: 1391 3216 4418 1391 Size: 1825 1202 4072 Fragments arranged by size: 4072 1825 1202 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2085 2319 3003 3125 928 Size: 971 186 234 684 122 4902 Fragments arranged by size: 4902 971 684 234 186 122 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2045 2079 3050 3084 5290 5324 1903 Size: 34 108 34 971 34 2206 34 3678 Fragments arranged by size: 3678 2206 971 108 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2137 4202 4991 6201 2137 Size: 2065 789 1210 3035 Fragments arranged by size: 3035 2065 1210 789 BclI T'GATC_A Cuts at: 1548 1548 Size: 7099 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5710 136 Size: 107 122 71 631 605 4038 1525 Fragments arranged by size: 4038 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2098 7094 2098 Size: 4996 2103 BlpI GC'TnA_GC Cuts at: 4516 4516 Size: 7099 Bme1580I G_kGCm'C Cuts at: 1790 4124 5160 6316 1790 Size: 2334 1036 1156 2573 Fragments arranged by size: 2573 2334 1156 1036 BmgBI CAC'GTC Cuts at: 1318 2353 1318 Size: 1035 6064 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3126 3405 4830 Size: 416 53 83 399 24 1709 279 1425 Cuts at: 4830 5788 442 Size: 958 1753 Fragments arranged by size: 1753 1709 1425 958 416 399 279 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2273 1056 Size: 1217 5882 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3759 5759 1239 Size: 2520 2000 2579 Fragments arranged by size: 2579 2520 2000 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2153 5224 6089 6387 2153 Size: 3071 865 298 2865 Fragments arranged by size: 3071 2865 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2498 3340 3777 5762 882 Size: 617 999 842 437 1985 2219 Fragments arranged by size: 2219 1985 999 842 617 437 BsaAI yAC'GTr Cuts at: 493 1204 4890 493 Size: 711 3686 2702 Fragments arranged by size: 3686 2702 711 BsaBI GATnn'nnATC Cuts at: 7093 7093 Size: 7099 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2617 4906 5288 5923 Size: 53 83 186 936 1084 2289 382 635 Cuts at: 5923 275 Size: 1451 Fragments arranged by size: 2289 1451 1084 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3446 5532 5930 6204 6351 6762 3446 Size: 2086 398 274 147 411 3783 Fragments arranged by size: 3783 2086 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2887 2917 3198 3228 1178 Size: 30 165 30 1484 30 281 30 5049 Fragments arranged by size: 5049 1484 281 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2655 2892 2895 3241 4130 1186 Size: 1469 237 3 346 889 4155 Fragments arranged by size: 4155 1469 889 346 237 3 BseYI C'CCAG_C Cuts at: 6302 6798 6302 Size: 496 6603 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2197 1163 Size: 573 461 6065 Fragments arranged by size: 6065 573 461 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4549 5310 6338 1540 Size: 275 186 2548 761 1028 2301 Fragments arranged by size: 2548 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3216 4124 5160 5245 6316 3216 Size: 908 1036 85 1071 3999 Fragments arranged by size: 3999 1071 1036 908 85 BsmI GAATG_Cn' Cuts at: 2489 4610 4703 2489 Size: 2121 93 4885 Fragments arranged by size: 4885 2121 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2343 2433 2343 Size: 90 7009 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3216 4124 4418 5160 5245 6316 1391 Size: 399 1426 908 294 742 85 1071 2174 Fragments arranged by size: 2174 1426 1071 908 742 399 294 85 BspEI T'CCGG_A Cuts at: 6762 6762 Size: 7099 BspHI T'CATG_A Cuts at: 4988 6718 4988 Size: 1730 5369 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3322 4212 4454 844 Size: 2478 890 242 3489 Fragments arranged by size: 3489 2478 890 242 BsrBI CCG'CTC Cuts at: 1394 1823 4986 1394 Size: 429 3163 3507 Fragments arranged by size: 3507 3163 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5594 5768 65 Size: 1183 4346 174 1396 Fragments arranged by size: 4346 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3446 5743 5930 1700 Size: 110 1636 2297 187 2869 Fragments arranged by size: 2869 2297 1636 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7099 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7099 BssSI C'ACGA_G Cuts at: 3107 3929 5159 6171 3107 Size: 822 1230 1012 4035 Fragments arranged by size: 4035 1230 1012 822 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7099 BstBI TT'CG_AA Cuts at: 5903 5903 Size: 7099 BstEII G'GTnAC_C Cuts at: 1184 3149 1184 Size: 1965 5134 BstYI r'GATC_y Cuts at: 1972 2098 3785 3797 4429 4796 5181 5198 6650 Size: 126 1687 12 632 367 385 17 1452 Cuts at: 6650 6736 6748 7094 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1687 1452 632 385 367 346 126 86 17 12 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3522 513 Size: 552 939 1518 4090 Fragments arranged by size: 4090 1518 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6360 Fragments arranged by size: 6360 683 56 BtsI GCAGTG_nn' Cuts at: 2185 2494 4615 5409 5429 2185 Size: 309 2121 794 20 3855 Fragments arranged by size: 3855 2121 794 309 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7099 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7064 DraI TTT'AAA Cuts at: 4403 4403 Size: 7099 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2797 6106 809 Size: 507 1481 3309 1802 Fragments arranged by size: 3309 1802 1481 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4546 5435 7064 Size: 54 1425 50 275 18 2716 889 1629 Cuts at: 7064 8 Size: 43 Fragments arranged by size: 2716 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4546 1537 Size: 275 2734 4090 Fragments arranged by size: 4090 2734 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2244 2415 2579 2787 3796 3935 4020 5029 Size: 891 171 164 208 1009 139 85 1009 Cuts at: 5029 1353 Size: 3423 Fragments arranged by size: 3423 1009 1009 891 208 171 164 139 85 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5686 6060 6206 387 Size: 1064 138 4097 374 146 1280 Fragments arranged by size: 4097 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3214 3214 Size: 7099 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2972 3759 3959 5162 5759 6546 6814 Size: 393 1340 787 200 1203 597 787 268 Cuts at: 6814 1239 Size: 1524 Fragments arranged by size: 1524 1340 1203 787 787 597 393 268 200 EcoO109I rG'GnC_Cy Cuts at: 3714 3714 Size: 7099 EcoRI G'AATT_C Cuts at: 2563 4408 4786 2563 Size: 1845 378 4876 Fragments arranged by size: 4876 1845 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2461 2461 Size: 7099 FspI TGC'GCA Cuts at: 4220 4394 5605 4220 Size: 174 1211 5714 Fragments arranged by size: 5714 1211 174 HaeII r_GCGC'y Cuts at: 1678 4033 6246 1678 Size: 2355 2213 2531 Fragments arranged by size: 2531 2355 2213 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2053 2578 2610 3236 Size: 32 135 32 449 32 525 32 626 Cuts at: 3236 3268 3641 3673 3688 3720 4289 4321 5740 Size: 32 373 32 15 32 569 32 1419 Cuts at: 5740 5772 5814 5846 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1419 626 569 525 449 373 135 42 32 32 32 32 32 32 32 32 32 32 15 HincII GTy'rAC Cuts at: 669 1657 1938 2597 4443 4689 669 Size: 988 281 659 1846 246 3079 Fragments arranged by size: 3079 1846 988 659 281 246 HindIII A'AGCT_T Cuts at: 748 4387 748 Size: 3639 3460 HpaI GTT'AAC Cuts at: 4689 4689 Size: 7099 KpnI G_GTAC'C Cuts at: 4424 4424 Size: 7099 MfeI C'AATT_G Cuts at: 2547 4020 4698 2547 Size: 1473 678 4948 Fragments arranged by size: 4948 1473 678 MluI A'CGCG_T Cuts at: 6975 6975 Size: 7099 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6213 6397 1305 Size: 4908 184 2007 Fragments arranged by size: 4908 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5277 Fragments arranged by size: 5277 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5057 5416 5575 518 Size: 648 3891 359 159 2042 Fragments arranged by size: 3891 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4515 5192 6340 6585 1606 Size: 2909 677 1148 245 2120 Fragments arranged by size: 2909 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3522 513 Size: 552 939 1518 4090 Fragments arranged by size: 4090 1518 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7099 NheI G'CTAG_C Cuts at: 1052 2269 1052 Size: 1217 5882 NotI GC'GGCC_GC Cuts at: 4546 4546 Size: 7099 NspI r_CATG'y Cuts at: 1132 1229 2020 4457 6002 1132 Size: 97 791 2437 1545 2229 Fragments arranged by size: 2437 2229 1545 791 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7099 PciI A'CATG_T Cuts at: 2016 5998 2016 Size: 3982 3117 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 6197 PmeI GTTT'AAAC Cuts at: 4403 4403 Size: 7099 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3856 3888 5168 5200 6681 6713 3856 Size: 32 1280 32 1481 32 4242 Fragments arranged by size: 4242 1481 1280 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3714 3714 Size: 7099 PshAI GACnn'nnGTC Cuts at: 1613 3730 1613 Size: 2117 4982 PsiI TTA'TAA Cuts at: 2478 3506 4669 2478 Size: 1028 1163 4908 Fragments arranged by size: 4908 1163 1028 PstI C_TGCA'G Cuts at: 830 2881 4451 830 Size: 2051 1570 3478 Fragments arranged by size: 3478 2051 1570 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7099 SacI G_AGCT'C Cuts at: 3216 3216 Size: 7099 SalI G'TCGA_C Cuts at: 1936 4441 1936 Size: 2505 4594 SapI GCTCTTCn'nnn_ Cuts at: 2415 4020 2415 Size: 1605 5494 SbfI CC_TGCA'GG Cuts at: 4451 4451 Size: 7099 ScaI AGT'ACT Cuts at: 5347 7059 5347 Size: 1712 5387 SfcI C'TryA_G Cuts at: 826 1045 2877 3826 3886 4447 5582 6263 6454 Size: 219 1832 949 60 561 1135 681 191 Cuts at: 6454 826 Size: 1471 Fragments arranged by size: 1832 1471 1135 949 681 561 219 191 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7099 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7099 SmaI CCC'GGG Cuts at: 1872 4426 1872 Size: 2554 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2168 2435 2854 5203 6104 6366 Size: 197 926 225 267 419 2349 901 262 Cuts at: 6366 820 Size: 1553 Fragments arranged by size: 2349 1553 926 901 419 267 262 225 197 SnaBI TAC'GTA Cuts at: 493 4890 493 Size: 4397 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7099 SphI G_CATG'C Cuts at: 1132 4457 1132 Size: 3325 3774 SspI AAT'ATT Cuts at: 5 52 5023 5 Size: 47 4971 2081 Fragments arranged by size: 4971 2081 47 StuI AGG'CCT Cuts at: 1517 2883 1517 Size: 1366 5733 StyI C'CwwG_G Cuts at: 513 1065 2004 2814 3522 3575 4538 513 Size: 552 939 810 708 53 963 3074 Fragments arranged by size: 3074 963 939 810 708 552 53 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2150 2908 3392 3679 5126 5143 5296 Size: 1255 570 758 484 287 1447 17 153 Cuts at: 5296 5481 5926 325 Size: 185 445 1498 Fragments arranged by size: 1498 1447 1255 758 570 484 445 287 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2208 4248 5345 Size: 274 80 33 51 1443 231 2040 1097 Cuts at: 5345 7057 96 Size: 1712 138 Fragments arranged by size: 2040 1712 1443 1097 274 231 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2887 2919 3198 3230 7059 7091 2887 Size: 32 279 32 3829 32 2895 Fragments arranged by size: 3829 2895 279 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7099 XbaI T'CTAG_A Cuts at: 3224 4435 3224 Size: 1211 5888 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7099 XmaI C'CCGG_G Cuts at: 1870 4424 1870 Size: 2554 4545 XmnI GAAnn'nnTTC Cuts at: 5228 5228 Size: 7099 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2618 4907 276 Size: 53 83 186 936 1084 2289 2468 Fragments arranged by size: 2468 2289 1084 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI