Restriction map for pFN21AB6281
 (Circular) (Six-base) MAPSORT of: pFN21AB6281.seq  Check: 7010  from: 1  to: 7099



pFN21AB6281  7099 bp

 With 182 enzymes: SgfI * 

                        January 31, 2011 17:47  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2620    4909     278 
   Size:         53      83     186     936    1084    2289    2468

  Fragments arranged by size: 

                2468    2289    1084     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    4442    1937 
   Size:       2505    4594
 
Acc65I G'GTAC_C

Cuts at:   4420    4420 
   Size:       7099
 
AclI AA'CG_TT

Cuts at:   1563    5226    5599    1563 
   Size:       3663     373    3063

  Fragments arranged by size: 

                3663    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2972    3959    5162    6546    6814    1632 
   Size:       1340     987    1203    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1340    1203     987     268
 
AflII C'TTAA_G

Cuts at:    820    1017    2435    2854     820 
   Size:        197    1418     419    5065

  Fragments arranged by size: 

                5065    1418     419     197
 
AflIII A'CryG_T

Cuts at:   1203    2016    5998    6975    1203 
   Size:        813    3982     977    1327

  Fragments arranged by size: 

                3982    1327     977     813
 
AgeI A'CCGG_T

Cuts at:   3446    5930    3446 
   Size:       2484    4615
 
AhdI GACnn_n'nnGTC

Cuts at:   2035    5828    2035 
   Size:       3793    3306
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       7099
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       7099
 
AlwNI CAG_nnn'CTG

Cuts at:   4842    6414    4842 
   Size:       1572    5527
 
ApaLI G'TGCA_C

Cuts at:   4120    5156    6312    4120 
   Size:       1036    1156    4907

  Fragments arranged by size: 

                4907    1156    1036
 
ApoI r'AATT_y

Cuts at:   1493    2563    3508    3847    4408    4634    4786    6895    1493 
   Size:       1070     945     339     561     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1070     945     561     339     226     152
            
 
AseI AT'TA_AT

Cuts at:    160    1032    3174    3198    5653     160 
   Size:        872    2142      24    2455    1606

  Fragments arranged by size: 

                2455    2142    1606     872      24
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7099
 
AvaI C'yCGr_G

Cuts at:   1870    1943    4424    1870 
   Size:         73    2481    4545

  Fragments arranged by size: 

                4545    2481      73
 
BamHI G'GATC_C

Cuts at:   4429    4796    4429 
   Size:        367    6732
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2396    2452    2974    4420    5875 
   Size:        325     217     627     609      56     522    1446    1455

Cuts at:   5875     618 
   Size:       1842

  Fragments arranged by size: 

                1842    1455    1446     627     609     522     325     217
                  56
 
BanII G_rGCy'C

Cuts at:   1391    3216    4418    1391 
   Size:       1825    1202    4072

  Fragments arranged by size: 

                4072    1825    1202
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2085    2319    3003    3125     928 
   Size:        971     186     234     684     122    4902

  Fragments arranged by size: 

                4902     971     684     234     186     122
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2045    2079    3050    3084    5290    5324    1903 
   Size:         34     108      34     971      34    2206      34    3678

  Fragments arranged by size: 

                3678    2206     971     108      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   2137    4202    4991    6201    2137 
   Size:       2065     789    1210    3035

  Fragments arranged by size: 

                3035    2065    1210     789
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       7099
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    5710     136 
   Size:        107     122      71     631     605    4038    1525

  Fragments arranged by size: 

                4038    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2098    7094    2098 
   Size:       4996    2103
 
BlpI GC'TnA_GC

Cuts at:   4516    4516 
   Size:       7099
 
Bme1580I G_kGCm'C

Cuts at:   1790    4124    5160    6316    1790 
   Size:       2334    1036    1156    2573

  Fragments arranged by size: 

                2573    2334    1156    1036
 
BmgBI CAC'GTC

Cuts at:   1318    2353    1318 
   Size:       1035    6064
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3126    3405    4830 
   Size:        416      53      83     399      24    1709     279    1425

Cuts at:   4830    5788     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753    1709    1425     958     416     399     279      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    2273    1056 
   Size:       1217    5882
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3759    5759    1239 
   Size:       2520    2000    2579

  Fragments arranged by size: 

                2579    2520    2000
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2153    5224    6089    6387    2153 
   Size:       3071     865     298    2865

  Fragments arranged by size: 

                3071    2865     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2498    3340    3777    5762     882 
   Size:        617     999     842     437    1985    2219

  Fragments arranged by size: 

                2219    1985     999     842     617     437
 
BsaAI yAC'GTr

Cuts at:    493    1204    4890     493 
   Size:        711    3686    2702

  Fragments arranged by size: 

                3686    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   7093    7093 
   Size:       7099
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2617    4906    5288    5923 
   Size:         53      83     186     936    1084    2289     382     635

Cuts at:   5923     275 
   Size:       1451

  Fragments arranged by size: 

                2289    1451    1084     936     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   3446    5532    5930    6204    6351    6762    3446 
   Size:       2086     398     274     147     411    3783

  Fragments arranged by size: 

                3783    2086     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2887    2917    3198    3228    1178 
   Size:         30     165      30    1484      30     281      30    5049

  Fragments arranged by size: 

                5049    1484     281     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2655    2892    2895    3241    4130    1186 
   Size:       1469     237       3     346     889    4155

  Fragments arranged by size: 

                4155    1469     889     346     237       3
 
BseYI C'CCAG_C

Cuts at:   6302    6798    6302 
   Size:        496    6603
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2197    1163 
   Size:        573     461    6065

  Fragments arranged by size: 

                6065     573     461
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    4549    5310    6338    1540 
   Size:        275     186    2548     761    1028    2301

  Fragments arranged by size: 

                2548    2301    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3216    4124    5160    5245    6316    3216 
   Size:        908    1036      85    1071    3999

  Fragments arranged by size: 

                3999    1071    1036     908      85
 
BsmI GAATG_Cn'

Cuts at:   2489    4610    4703    2489 
   Size:       2121      93    4885

  Fragments arranged by size: 

                4885    2121      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2343    2433    2343 
   Size:         90    7009
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    3216    4124    4418    5160    5245    6316    1391 
   Size:        399    1426     908     294     742      85    1071    2174

  Fragments arranged by size: 

                2174    1426    1071     908     742     399     294      85
            
 
BspEI T'CCGG_A

Cuts at:   6762    6762 
   Size:       7099
 
BspHI T'CATG_A

Cuts at:   4988    6718    4988 
   Size:       1730    5369
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3322    4212    4454     844 
   Size:       2478     890     242    3489

  Fragments arranged by size: 

                3489    2478     890     242
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4986    1394 
   Size:        429    3163    3507

  Fragments arranged by size: 

                3507    3163     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    5594    5768      65 
   Size:       1183    4346     174    1396

  Fragments arranged by size: 

                4346    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3446    5743    5930    1700 
   Size:        110    1636    2297     187    2869

  Fragments arranged by size: 

                2869    2297    1636     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       7099
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       7099
 
BssSI C'ACGA_G

Cuts at:   3107    3929    5159    6171    3107 
   Size:        822    1230    1012    4035

  Fragments arranged by size: 

                4035    1230    1012     822
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       7099
 
BstBI TT'CG_AA

Cuts at:   5903    5903 
   Size:       7099
 
BstEII G'GTnAC_C

Cuts at:   1184    3149    1184 
   Size:       1965    5134
 
BstYI r'GATC_y

Cuts at:   1972    2098    3785    3797    4429    4796    5181    5198    6650 
   Size:        126    1687      12     632     367     385      17    1452

Cuts at:   6650    6736    6748    7094    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1687    1452     632     385     367     346     126
                  86      17      12      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    3522     513 
   Size:        552     939    1518    4090

  Fragments arranged by size: 

                4090    1518     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    6360

  Fragments arranged by size: 

                6360     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2185    2494    4615    5409    5429    2185 
   Size:        309    2121     794      20    3855

  Fragments arranged by size: 

                3855    2121     794     309      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       7099
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    7064
 
DraI TTT'AAA

Cuts at:   4403    4403 
   Size:       7099
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2797    6106     809 
   Size:        507    1481    3309    1802

  Fragments arranged by size: 

                3309    1802    1481     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    4546    5435    7064 
   Size:         54    1425      50     275      18    2716     889    1629

Cuts at:   7064       8 
   Size:         43

  Fragments arranged by size: 

                2716    1629    1425     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    4546    1537 
   Size:        275    2734    4090

  Fragments arranged by size: 

                4090    2734     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2244    2415    2579    2787    3796    3935    4020    5029 
   Size:        891     171     164     208    1009     139      85    1009

Cuts at:   5029    1353 
   Size:       3423

  Fragments arranged by size: 

                3423    1009    1009     891     208     171     164     139
                  85
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    5686    6060    6206     387 
   Size:       1064     138    4097     374     146    1280

  Fragments arranged by size: 

                4097    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   3214    3214 
   Size:       7099
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2972    3759    3959    5162    5759    6546    6814 
   Size:        393    1340     787     200    1203     597     787     268

Cuts at:   6814    1239 
   Size:       1524

  Fragments arranged by size: 

                1524    1340    1203     787     787     597     393     268
                 200
 
EcoO109I rG'GnC_Cy

Cuts at:   3714    3714 
   Size:       7099
 
EcoRI G'AATT_C

Cuts at:   2563    4408    4786    2563 
   Size:       1845     378    4876

  Fragments arranged by size: 

                4876    1845     378
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2461    2461 
   Size:       7099
 
FspI TGC'GCA

Cuts at:   4220    4394    5605    4220 
   Size:        174    1211    5714

  Fragments arranged by size: 

                5714    1211     174
 
HaeII r_GCGC'y

Cuts at:   1678    4033    6246    1678 
   Size:       2355    2213    2531

  Fragments arranged by size: 

                2531    2355    2213
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2021    2053    2578    2610    3236 
   Size:         32     135      32     449      32     525      32     626

Cuts at:   3236    3268    3641    3673    3688    3720    4289    4321    5740 
   Size:         32     373      32      15      32     569      32    1419

Cuts at:   5740    5772    5814    5846    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1419     626     569     525     449     373     135
                  42      32      32      32      32      32      32      32
                  32      32      32      15
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2597    4443    4689     669 
   Size:        988     281     659    1846     246    3079

  Fragments arranged by size: 

                3079    1846     988     659     281     246
 
HindIII A'AGCT_T

Cuts at:    748    4387     748 
   Size:       3639    3460
 
HpaI GTT'AAC

Cuts at:   4689    4689 
   Size:       7099
 
KpnI G_GTAC'C

Cuts at:   4424    4424 
   Size:       7099
 
MfeI C'AATT_G

Cuts at:   2547    4020    4698    2547 
   Size:       1473     678    4948

  Fragments arranged by size: 

                4948    1473     678
 
MluI A'CGCG_T

Cuts at:   6975    6975 
   Size:       7099
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    6213    6397    1305 
   Size:       4908     184    2007

  Fragments arranged by size: 

                4908    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    5277

  Fragments arranged by size: 

                5277    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    5057    5416    5575     518 
   Size:        648    3891     359     159    2042

  Fragments arranged by size: 

                3891    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    4515    5192    6340    6585    1606 
   Size:       2909     677    1148     245    2120

  Fragments arranged by size: 

                2909    2120    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    3522     513 
   Size:        552     939    1518    4090

  Fragments arranged by size: 

                4090    1518     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       7099
 
NheI G'CTAG_C

Cuts at:   1052    2269    1052 
   Size:       1217    5882
 
NotI GC'GGCC_GC

Cuts at:   4546    4546 
   Size:       7099
 
NspI r_CATG'y

Cuts at:   1132    1229    2020    4457    6002    1132 
   Size:         97     791    2437    1545    2229

  Fragments arranged by size: 

                2437    2229    1545     791      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       7099
 
PciI A'CATG_T

Cuts at:   2016    5998    2016 
   Size:       3982    3117
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2010    1108 
   Size:        902    6197
 
PmeI GTTT'AAAC

Cuts at:   4403    4403 
   Size:       7099
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3856    3888    5168    5200    6681    6713    3856 
   Size:         32    1280      32    1481      32    4242

  Fragments arranged by size: 

                4242    1481    1280      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   3714    3714 
   Size:       7099
 
PshAI GACnn'nnGTC

Cuts at:   1613    3730    1613 
   Size:       2117    4982
 
PsiI TTA'TAA

Cuts at:   2478    3506    4669    2478 
   Size:       1028    1163    4908

  Fragments arranged by size: 

                4908    1163    1028
 
PstI C_TGCA'G

Cuts at:    830    2881    4451     830 
   Size:       2051    1570    3478

  Fragments arranged by size: 

                3478    2051    1570
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       7099
 
SacI G_AGCT'C

Cuts at:   3216    3216 
   Size:       7099
 
SalI G'TCGA_C

Cuts at:   1936    4441    1936 
   Size:       2505    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2415    4020    2415 
   Size:       1605    5494
 
SbfI CC_TGCA'GG

Cuts at:   4451    4451 
   Size:       7099
 
ScaI AGT'ACT

Cuts at:   5347    7059    5347 
   Size:       1712    5387
 
SfcI C'TryA_G

Cuts at:    826    1045    2877    3826    3886    4447    5582    6263    6454 
   Size:        219    1832     949      60     561    1135     681     191

Cuts at:   6454     826 
   Size:       1471

  Fragments arranged by size: 

                1832    1471    1135     949     681     561     219     191
                  60
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7099
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       7099
 
SmaI CCC'GGG

Cuts at:   1872    4426    1872 
   Size:       2554    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2168    2435    2854    5203    6104    6366 
   Size:        197     926     225     267     419    2349     901     262

Cuts at:   6366     820 
   Size:       1553

  Fragments arranged by size: 

                2349    1553     926     901     419     267     262     225
                 197
 
SnaBI TAC'GTA

Cuts at:    493    4890     493 
   Size:       4397    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       7099
 
SphI G_CATG'C

Cuts at:   1132    4457    1132 
   Size:       3325    3774
 
SspI AAT'ATT

Cuts at:      5      52    5023       5 
   Size:         47    4971    2081

  Fragments arranged by size: 

                4971    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2883    1517 
   Size:       1366    5733
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2814    3522    3575    4538     513 
   Size:        552     939     810     708      53     963    3074

  Fragments arranged by size: 

                3074     963     939     810     708     552      53
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2150    2908    3392    3679    5126    5143    5296 
   Size:       1255     570     758     484     287    1447      17     153

Cuts at:   5296    5481    5926     325 
   Size:        185     445    1498

  Fragments arranged by size: 

                1498    1447    1255     758     570     484     445     287
                 185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2208    4248    5345 
   Size:        274      80      33      51    1443     231    2040    1097

Cuts at:   5345    7057      96 
   Size:       1712     138

  Fragments arranged by size: 

                2040    1712    1443    1097     274     231     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2887    2919    3198    3230    7059    7091    2887 
   Size:         32     279      32    3829      32    2895

  Fragments arranged by size: 

                3829    2895     279      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       7099
 
XbaI T'CTAG_A

Cuts at:   3224    4435    3224 
   Size:       1211    5888
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       7099
 
XmaI C'CCGG_G

Cuts at:   1870    4424    1870 
   Size:       2554    4545
 
XmnI GAAnn'nnTTC

Cuts at:   5228    5228 
   Size:       7099
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2618    4907     276 
   Size:         53      83     186     936    1084    2289    2468

  Fragments arranged by size: 

                2468    2289    1084     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BstXI  BstZ17I   Bsu36I
   DraIII    EcoNI    EcoRV     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI   PspOMI    PspXI
     PsrI    PvuII    RsrII    SacII    SanDI    SexAI     SfiI     SfoI
     SrfI     SwaI     XcmI
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