Restriction map for pFN21AB6281
 (Linear) (Six-base) MAPSORT of: KIBB6281.seq  Check: 5236  from: 1  to: 2394



KIBB6281.orf

 With 182 enzymes: SgfI * 

                        June 25, 2015 11:41  ..

AatII G_ACGT'C

Cuts at:      0     615    2394 
   Size:        615    1779
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     967    1954    2394 
   Size:        967     987     440

  Fragments arranged by size: 

                 987     967     440
 
AflII C'TTAA_G

Cuts at:      0     430     849    2394 
   Size:        430     419    1545

  Fragments arranged by size: 

                1545     430     419
 
AflIII A'CryG_T

Cuts at:      0      11    2394 
   Size:         11    2383
 
AgeI A'CCGG_T

Cuts at:      0    1441    2394 
   Size:       1441     953
 
AhdI GACnn_n'nnGTC

Cuts at:      0      30    2394 
   Size:         30    2364
 
ApaLI G'TGCA_C

Cuts at:      0    2115    2394 
   Size:       2115     279
 
ApoI r'AATT_y

Cuts at:      0     558    1503    1842    2394 
   Size:        558     945     339     552

  Fragments arranged by size: 

                 945     558     552     339
 
AseI AT'TA_AT

Cuts at:      0    1169    1193    2394 
   Size:       1169      24    1201

  Fragments arranged by size: 

                1201    1169      24
 
BanI G'GyrC_C

Cuts at:      0     391     447     969    2394 
   Size:        391      56     522    1425

  Fragments arranged by size: 

                1425     522     391      56
 
BanII G_rGCy'C

Cuts at:      0    1211    2394 
   Size:       1211    1183
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      80     314     998    1120    2394 
   Size:         80     234     684     122    1274

  Fragments arranged by size: 

                1274     684     234     122      80
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      40      74    1045    1079    2394 
   Size:         40      34     971      34    1315

  Fragments arranged by size: 

                1315     971      40      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     132    2197    2394 
   Size:        132    2065     197

  Fragments arranged by size: 

                2065     197     132
 
BglII A'GATC_T

Cuts at:      0      93    2394 
   Size:         93    2301
 
Bme1580I G_kGCm'C

Cuts at:      0    2119    2394 
   Size:       2119     275
 
BmgBI CAC'GTC

Cuts at:      0     348    2394 
   Size:        348    2046
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1121    1400    2394 
   Size:       1121     279     994

  Fragments arranged by size: 

                1121     994     279
 
BmtI G_CTAG'C

Cuts at:      0     268    2394 
   Size:        268    2126
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1754    2394 
   Size:       1754     640
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148    2394 
   Size:        148    2246
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     493    1335    1772    2394 
   Size:        493     842     437     622

  Fragments arranged by size: 

                 842     622     493     437
 
BsaHI Gr'CG_yC

Cuts at:      0     612    2394 
   Size:        612    1782
 
BsaWI w'CCGG_w

Cuts at:      0    1441    2394 
   Size:       1441     953
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     882     912    1193    1223    2394 
   Size:        882      30     281      30    1171

  Fragments arranged by size: 

                1171     882     281      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     650     887     890    1236    2125    2394 
   Size:        650     237       3     346     889     269

  Fragments arranged by size: 

                 889     650     346     269     237       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     192    2394 
   Size:        192    2202
 
BsiHKAI G_wGCw'C

Cuts at:      0    1211    2119    2394 
   Size:       1211     908     275

  Fragments arranged by size: 

                1211     908     275
 
BsmI GAATG_Cn'

Cuts at:      0     484    2394 
   Size:        484    1910
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     338     428    2394 
   Size:        338      90    1966

  Fragments arranged by size: 

                1966     338      90
 
Bsp1286I G_dGCh'C

Cuts at:      0    1211    2119    2394 
   Size:       1211     908     275

  Fragments arranged by size: 

                1211     908     275
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1317    2207    2394 
   Size:       1317     890     187

  Fragments arranged by size: 

                1317     890     187
 
BsrFI r'CCGG_y

Cuts at:      0    1441    2394 
   Size:       1441     953
 
BssSI C'ACGA_G

Cuts at:      0    1102    1924    2394 
   Size:       1102     822     470

  Fragments arranged by size: 

                1102     822     470
 
BstEII G'GTnAC_C

Cuts at:      0    1144    2394 
   Size:       1144    1250
 
BstYI r'GATC_y

Cuts at:      0      93    1780    1792    2394 
   Size:         93    1687      12     602

  Fragments arranged by size: 

                1687     602      93      12
 
BtgI C'CryG_G

Cuts at:      0    1517    2394 
   Size:       1517     877
 
BtsI GCAGTG_nn'

Cuts at:      0     180     489    2394 
   Size:        180     309    1905

  Fragments arranged by size: 

                1905     309     180
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     792    2394 
   Size:        792    1602
 
EarI CTCTTCn'nnn_

Cuts at:      0     239     410     574     782    1791    1930    2015    2394 
   Size:        239     171     164     208    1009     139      85     379

  Fragments arranged by size: 

                1009     379     239     208     171     164     139      85
            
 
EcoICRI GAG'CTC

Cuts at:      0    1209    2394 
   Size:       1209    1185
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     967    1754    1954    2394 
   Size:        967     787     200     440

  Fragments arranged by size: 

                 967     787     440     200
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1709    2394 
   Size:       1709     685
 
EcoRI G'AATT_C

Cuts at:      0     558    2394 
   Size:        558    1836
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     456    2394 
   Size:        456    1938
 
FspI TGC'GCA

Cuts at:      0    2215    2389    2394 
   Size:       2215     174       5

  Fragments arranged by size: 

                2215     174       5
 
HaeII r_GCGC'y

Cuts at:      0    2028    2394 
   Size:       2028     366
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      16      48     573     605    1231    1263    1636    1668 
   Size:         16      32     525      32     626      32     373      32

Cuts at:   1668    1683    1715    2284    2316    2394 
   Size:         15      32     569      32      78

  Fragments arranged by size: 

                 626     569     525     373      78      32      32      32
                  32      32      32      16      15
 
HincII GTy'rAC

Cuts at:      0     592    2394 
   Size:        592    1802
 
HindIII A'AGCT_T

Cuts at:      0    2382    2394 
   Size:       2382      12
 
MfeI C'AATT_G

Cuts at:      0     542    2015    2394 
   Size:        542    1473     379

  Fragments arranged by size: 

                1473     542     379
 
NcoI C'CATG_G

Cuts at:      0    1517    2394 
   Size:       1517     877
 
NheI G'CTAG_C

Cuts at:      0     264    2394 
   Size:        264    2130
 
NspI r_CATG'y

Cuts at:      0      15    2394 
   Size:         15    2379
 
PciI A'CATG_T

Cuts at:      0      11    2394 
   Size:         11    2383
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1851    1883    2394 
   Size:       1851      32     511

  Fragments arranged by size: 

                1851     511      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1709    2394 
   Size:       1709     685
 
PshAI GACnn'nnGTC

Cuts at:      0    1725    2394 
   Size:       1725     669
 
PsiI TTA'TAA

Cuts at:      0     473    1501    2394 
   Size:        473    1028     893

  Fragments arranged by size: 

                1028     893     473
 
PstI C_TGCA'G

Cuts at:      0     876    2394 
   Size:        876    1518
 
SacI G_AGCT'C

Cuts at:      0    1211    2394 
   Size:       1211    1183
 
SapI GCTCTTCn'nnn_

Cuts at:      0     410    2015    2394 
   Size:        410    1605     379

  Fragments arranged by size: 

                1605     410     379
 
SfcI C'TryA_G

Cuts at:      0     872    1821    1881    2394 
   Size:        872     949      60     513

  Fragments arranged by size: 

                 949     872     513      60
 
SmlI C'TyrA_G

Cuts at:      0     163     430     849    2394 
   Size:        163     267     419    1545

  Fragments arranged by size: 

                1545     419     267     163
 
StuI AGG'CCT

Cuts at:      0     878    2394 
   Size:        878    1516
 
StyI C'CwwG_G

Cuts at:      0     809    1517    1570    2394 
   Size:        809     708      53     824

  Fragments arranged by size: 

                 824     809     708      53
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     145     903    1387    1674    2394 
   Size:        145     758     484     287     720

  Fragments arranged by size: 

                 758     720     484     287     145
 
TatI w'GTAC_w

Cuts at:      0     203    2243    2394 
   Size:        203    2040     151

  Fragments arranged by size: 

                2040     203     151
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     882     914    1193    1225    2394 
   Size:        882      32     279      32    1169

  Fragments arranged by size: 

                1169     882     279      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1219    2394 
   Size:       1219    1175
 
ZraI GAC'GTC

Cuts at:      0     613    2394 
   Size:        613    1781
 

 Enzymes that do cut:

    AatII     AcuI    AflII   AflIII     AgeI     AhdI    ApaLI     ApoI
     AseI     BanI    BanII     BbsI     BcgI    BciVI    BglII Bme1580I
    BmgBI     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI
    BsrFI    BssSI   BstEII    BstYI     BtgI     BtsI     DrdI     EarI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     MfeI     NcoI     NheI     NspI     PciI     PpiI
    PpuMI    PshAI     PsiI     PstI     SacI     SapI     SfcI     SmlI
     StuI     StyI    TaqII     TatI     TstI     XbaI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI     AleI     AlfI     AloI
    AlwNI     ApaI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BclI    BfrBI     BglI     BlpI     BplI   Bpu10I
    BsaAI    BsaBI    BseYI    BsiEI    BsiWI    BspEI    BspHI    BsrBI
    BsrDI    BsrGI   BssHII   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EciI
    EcoNI    EcoRV     FseI    FspAI     HpaI     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
  Tth111I     XcmI     XhoI     XmaI     XmnI
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