Restriction map for
pFN21AB5987
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsmBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FauI FokI FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 1385 GACGCC G[GA]^CG[CT]C
AflIII 631 ACATGT A^C[GA][CT]GT
AlwNI 1608 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 388 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1631 TGGCCA TGG^CCA
BclI 747 TGATCA T^GATCA
BsaAI 1414 TACGTG [CT]AC^GT[GA]
BsaHI 1385 GACGCC G[GA]^CG[CT]C
BsiWI 661 CGTACG C^GTACG
BspLU11I 631 ACATGT A^CATGT
BstXI 183 CCATTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 972 GGATCT [GA]^GATC[CT]
CfoI 981 GCGC GCG^C
DsaI 1275 CCGTGG C^C[GA][CT]GG
EaeI 1631 TGGCCA [CT]^GGCC[GA]
EarI 1542 GAAGAG CTCTTC(1/4)
EcoNI 1229 CCTATGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 747 TGATCA T^GATCA
HgaI 1385 GACGC GACGC(5/10)
HhaI 981 GCGC GCG^C
Hin1I 1385 GACGCC G[GA]^CG[CT]C
HinP1I 981 GCGC G^CGC
Ksp632I 1542 GAAGAG CTCTTC(1/4)
MflI 972 GGATCT [GA]^GATC[CT]
MluNI 1631 TGGCCA TGG^CCA
MscI 1631 TGGCCA TGG^CCA
PflMI 649 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 230 GGGACCT [GA]G^G[AT]CC[CT]
PstI 1624 CTGCAG CTGCA^G
SacI 1444 GAGCTC GAGCT^C
Van91I 649 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 972 GGATCT [GA]^GATC[CT]
XmnI 388 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaI 262 CCCGAG C^[CT]CG[GA]G
1475 CTCGGG
BanII 1169 GGGCTC G[GA]GC[CT]^C
1444 GAGCTC
BcnI 228 CCGGG CC^[GC]GG
1167 CCGGG
BpmI 1420 CTCCAG CTGGAG(16/14)
1441 CTGGAG
BsmI 1239 GAATGC GAATGC(1/-1)
461 GCATTC
BsoBI 262 CCCGAG C^[CT]CG[GA]G
1475 CTCGGG
BsrDI 600 CATTGC GCAATG(2/0)
830 GCAATG
DraII 230 GGGACCT [GA]G^G[ACGT]CC[CT]
1679 AGGGCCT
EcoO109I 230 GGGACCT [GA]G^G[ACGT]CC[CT]
1679 AGGGCCT
EcoT14I 383 CCTTGG C^C[AT][AT]GG
1034 CCAAGG
MslI 5 CACTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1635 CATGCCAGTG
NciI 228 CCGGG CC^[GC]GG
1167 CCGGG
NspI 341 GCATGT [GA]CATG^[CT]
631 ACATGT
PleI 681 GACTC GAGTC(4/5)
24 GAGTC
SfcI 171 CTACAG C^T[GA][CT]AG
1624 CTGCAG
StyI 383 CCTTGG C^C[AT][AT]GG
1034 CCAAGG
Tsp45I 886 GTCAC ^GT[GC]AC
1348 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbvI BciVI
BfaI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstAPI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI TfiI Tru9I
TseI Tsp509I TspRI