(Circular) (Six-base) MAPSORT of: pFN21AB5987.seq Check: 2164 from: 1 to: 6415 pFN21AB5987 6415 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3622 3622 Size: 6415 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4225 278 Size: 53 83 186 936 2689 2468 Fragments arranged by size: 2689 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3758 1937 Size: 1821 4594 Acc65I G'GTAC_C Cuts at: 3736 3736 Size: 6415 AclI AA'CG_TT Cuts at: 1563 4542 4915 1563 Size: 2979 373 3063 Fragments arranged by size: 3063 2979 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3566 4478 5862 6130 1632 Size: 1934 912 1384 268 1917 Fragments arranged by size: 1934 1917 1384 912 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6218 AflIII A'CryG_T Cuts at: 1203 2636 5314 6291 1203 Size: 1433 2678 977 1327 Fragments arranged by size: 2678 1433 1327 977 AgeI A'CCGG_T Cuts at: 5246 5246 Size: 6415 AhdI GACnn_n'nnGTC Cuts at: 5144 5144 Size: 6415 AleI CACnn'nnGTG Cuts at: 1166 2014 1166 Size: 848 5567 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2076 1184 Size: 892 5523 AlwNI CAG_nnn'CTG Cuts at: 3618 4158 5730 3618 Size: 540 1572 4303 Fragments arranged by size: 4303 1572 540 ApaLI G'TGCA_C Cuts at: 4472 5628 4472 Size: 1156 5259 ApoI r'AATT_y Cuts at: 1493 2168 2560 3260 3299 3724 3950 4102 6211 Size: 675 392 700 39 425 226 152 2109 Cuts at: 6211 1493 Size: 1697 Fragments arranged by size: 2109 1697 700 675 425 392 226 152 39 AseI AT'TA_AT Cuts at: 160 1032 4969 160 Size: 872 3937 1606 Fragments arranged by size: 3937 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6415 AvaI C'yCGr_G Cuts at: 1870 1943 2267 3480 3740 1870 Size: 73 324 1213 260 4545 Fragments arranged by size: 4545 1213 324 260 73 BamHI G'GATC_C Cuts at: 3745 4112 3745 Size: 367 6048 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3736 5191 618 Size: 325 217 627 1949 1455 1842 Fragments arranged by size: 1949 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3178 3453 3734 1391 Size: 1787 275 281 4072 Fragments arranged by size: 4072 1787 281 275 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5444 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4606 4640 1903 Size: 34 2669 34 3678 Fragments arranged by size: 3678 2669 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3241 3299 3612 4307 5517 3241 Size: 58 313 695 1210 4139 Fragments arranged by size: 4139 1210 695 313 58 BclI T'GATC_A Cuts at: 1548 2752 1548 Size: 1204 5211 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5026 136 Size: 107 122 71 631 605 3354 1525 Fragments arranged by size: 3354 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6410 6410 Size: 6415 BlpI GC'TnA_GC Cuts at: 3832 3832 Size: 6415 Bme1580I G_kGCm'C Cuts at: 1790 2147 2730 4476 5632 1790 Size: 357 583 1746 1156 2573 Fragments arranged by size: 2573 1746 1156 583 357 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6415 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2092 2432 4146 Size: 416 53 83 399 24 675 340 1714 Cuts at: 4146 5104 442 Size: 958 1753 Fragments arranged by size: 1753 1714 958 675 416 399 340 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6415 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3410 3467 5075 1239 Size: 2171 57 1608 2579 Fragments arranged by size: 2579 2171 1608 57 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2261 2614 2966 3437 4540 5405 5703 2261 Size: 353 352 471 1103 865 298 2973 Fragments arranged by size: 2973 1103 865 471 353 352 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5078 882 Size: 617 3579 2219 Fragments arranged by size: 3579 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3421 4206 493 Size: 711 2217 785 2702 Fragments arranged by size: 2702 2217 785 711 BsaBI GATnn'nnATC Cuts at: 6409 6409 Size: 6415 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3391 4222 4604 5239 Size: 53 83 186 936 1858 831 382 635 Cuts at: 5239 275 Size: 1451 Fragments arranged by size: 1858 1451 936 831 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4848 5246 5520 5667 6078 4848 Size: 398 274 147 411 5185 Fragments arranged by size: 5185 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3090 3120 3261 3291 1178 Size: 30 165 30 1687 30 141 30 4302 Fragments arranged by size: 4302 1687 165 141 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2258 2309 2798 1186 Size: 1072 51 489 4803 Fragments arranged by size: 4803 1072 489 51 BseYI C'CCAG_C Cuts at: 5618 6114 5618 Size: 496 5919 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5842 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3865 4626 5654 1540 Size: 275 186 1864 761 1028 2301 Fragments arranged by size: 2301 1864 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3426 3453 3567 4476 4561 5632 3426 Size: 27 114 909 85 1071 4209 Fragments arranged by size: 4209 1071 909 114 85 27 BsiWI C'GTAC_G Cuts at: 2666 2666 Size: 6415 BsmI GAATG_Cn' Cuts at: 2466 3250 3926 4019 2466 Size: 784 676 93 4862 Fragments arranged by size: 4862 784 676 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2147 2730 3178 3426 3453 3567 3734 Size: 399 357 583 448 248 27 114 167 Cuts at: 3734 4476 4561 5632 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 583 448 399 357 248 167 114 85 27 BspEI T'CCGG_A Cuts at: 6078 6078 Size: 6415 BspHI T'CATG_A Cuts at: 4304 6034 4304 Size: 1730 4685 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2351 3603 3622 3770 844 Size: 1507 1252 19 148 3489 Fragments arranged by size: 3489 1507 1252 148 19 BsrBI CCG'CTC Cuts at: 1394 1823 4302 1394 Size: 429 2479 3507 Fragments arranged by size: 3507 2479 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2604 2842 4910 5084 65 Size: 1183 1356 238 2068 174 1396 Fragments arranged by size: 2068 1396 1356 1183 238 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5059 5246 1700 Size: 110 3249 187 2869 Fragments arranged by size: 3249 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6415 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6415 BssSI C'ACGA_G Cuts at: 4475 5487 4475 Size: 1012 5403 BstAPI GCAn_nnn'nTGC Cuts at: 794 2142 3055 3245 794 Size: 1348 913 190 3964 Fragments arranged by size: 3964 1348 913 190 BstBI TT'CG_AA Cuts at: 5219 5219 Size: 6415 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6415 BstXI CCAn_nnnn'nTGG Cuts at: 2195 2195 Size: 6415 BstYI r'GATC_y Cuts at: 1972 2977 3745 4112 4497 4514 5966 6052 6064 Size: 1005 768 367 385 17 1452 86 12 Cuts at: 6064 6410 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1005 768 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3280 513 Size: 552 2215 3648 Fragments arranged by size: 3648 2215 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2091 487 Size: 683 56 865 4811 Fragments arranged by size: 4811 865 683 56 BtsI GCAGTG_nn' Cuts at: 2730 3679 3931 4725 4745 2730 Size: 949 252 794 20 4400 Fragments arranged by size: 4400 949 794 252 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6415 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6380 DraI TTT'AAA Cuts at: 3719 3719 Size: 6415 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5422 809 Size: 507 4106 1802 Fragments arranged by size: 4106 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3636 3862 4751 Size: 54 1425 50 275 18 1806 226 889 Cuts at: 4751 6380 8 Size: 1629 43 Fragments arranged by size: 1806 1629 1425 889 275 226 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3862 1537 Size: 275 2050 4090 Fragments arranged by size: 4090 2050 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3542 4345 1353 Size: 2189 803 3423 Fragments arranged by size: 3423 2189 803 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2249 5002 5376 5522 387 Size: 1064 138 660 2753 374 146 1280 Fragments arranged by size: 2753 1280 1064 660 374 146 138 EcoICRI GAG'CTC Cuts at: 3451 3451 Size: 6415 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3410 3467 3566 4478 5075 5862 6130 Size: 393 1778 57 99 912 597 787 268 Cuts at: 6130 1239 Size: 1524 Fragments arranged by size: 1778 1524 912 787 597 393 268 99 57 EcoNI CCTnn'n_nnAGG Cuts at: 3238 3238 Size: 6415 EcoO109I rG'GnC_Cy Cuts at: 2236 3685 2236 Size: 1449 4966 EcoRI G'AATT_C Cuts at: 3724 4102 3724 Size: 378 6037 FspI TGC'GCA Cuts at: 4921 4921 Size: 6415 HaeII r_GCGC'y Cuts at: 1678 5562 1678 Size: 3884 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2467 2499 2815 2847 3090 Size: 32 135 32 895 32 316 32 243 Cuts at: 3090 3122 5056 5088 5130 5162 1373 Size: 32 1934 32 42 32 2626 Fragments arranged by size: 2626 1934 895 316 243 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3759 4005 669 Size: 988 281 1821 246 3079 Fragments arranged by size: 3079 1821 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6415 HpaI GTT'AAC Cuts at: 4005 4005 Size: 6415 KpnI G_GTAC'C Cuts at: 3740 3740 Size: 6415 MfeI C'AATT_G Cuts at: 4014 4014 Size: 6415 MluI A'CGCG_T Cuts at: 6291 6291 Size: 6415 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5529 5713 1305 Size: 4224 184 2007 Fragments arranged by size: 4224 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3638 10 Size: 54 1425 343 1806 2787 Fragments arranged by size: 2787 1806 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2014 3644 4373 4732 4891 518 Size: 648 848 1630 729 359 159 2042 Fragments arranged by size: 2042 1630 848 729 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2195 2412 3628 3831 4508 5656 5901 1606 Size: 589 217 1216 203 677 1148 245 2120 Fragments arranged by size: 2120 1216 1148 677 589 245 217 203 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5863 NdeI CA'TA_TG Cuts at: 387 387 Size: 6415 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6415 NotI GC'GGCC_GC Cuts at: 3862 3862 Size: 6415 NspI r_CATG'y Cuts at: 1132 1229 2350 2640 3773 5318 1132 Size: 97 1121 290 1133 1545 2229 Fragments arranged by size: 2229 1545 1133 1121 290 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6415 PciI A'CATG_T Cuts at: 2636 5314 2636 Size: 2678 3737 PflMI CCAn_nnn'nTGG Cuts at: 1108 2660 1108 Size: 1552 4863 PfoI T'CCnGG_A Cuts at: 3444 3444 Size: 6415 PmeI GTTT'AAAC Cuts at: 3719 3719 Size: 6415 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4484 4516 5997 6029 4484 Size: 32 1481 32 4870 Fragments arranged by size: 4870 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2236 2236 Size: 6415 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6415 PsiI TTA'TAA Cuts at: 2550 3985 2550 Size: 1435 4980 PstI C_TGCA'G Cuts at: 830 3633 3767 830 Size: 2803 134 3478 Fragments arranged by size: 3478 2803 134 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6415 PvuII CAG'CTG Cuts at: 2195 2412 3628 2195 Size: 217 1216 4982 Fragments arranged by size: 4982 1216 217 SacI G_AGCT'C Cuts at: 3453 3453 Size: 6415 SalI G'TCGA_C Cuts at: 1936 3757 1936 Size: 1821 4594 SbfI CC_TGCA'GG Cuts at: 3767 3767 Size: 6415 ScaI AGT'ACT Cuts at: 4663 6375 4663 Size: 1712 4703 SfcI C'TryA_G Cuts at: 826 1045 2176 3629 3763 4898 5579 5770 826 Size: 219 1131 1453 134 1135 681 191 1471 Fragments arranged by size: 1471 1453 1135 1131 681 219 191 134 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6415 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6415 SmaI CCC'GGG Cuts at: 1872 3742 1872 Size: 1870 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2240 2593 2981 3452 4519 5420 Size: 197 926 297 353 388 471 1067 901 Cuts at: 5420 5682 820 Size: 262 1553 Fragments arranged by size: 1553 1067 926 901 471 388 353 297 262 197 SnaBI TAC'GTA Cuts at: 493 4206 493 Size: 3713 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6415 SphI G_CATG'C Cuts at: 1132 3773 1132 Size: 2641 3774 SspI AAT'ATT Cuts at: 5 52 4339 5 Size: 47 4287 2081 Fragments arranged by size: 4287 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6415 StyI C'CwwG_G Cuts at: 513 1065 2388 3039 3854 513 Size: 552 1323 651 815 3074 Fragments arranged by size: 3074 1323 815 651 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3342 4442 4459 4612 4797 5242 325 Size: 1255 1762 1100 17 153 185 445 1498 Fragments arranged by size: 1762 1498 1255 1100 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3505 3510 4661 Size: 274 80 33 51 1443 1528 5 1151 Cuts at: 4661 6373 96 Size: 1712 138 Fragments arranged by size: 1712 1528 1443 1151 274 138 80 51 33 5 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6375 6407 6375 Size: 32 6383 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6415 XbaI T'CTAG_A Cuts at: 3751 3751 Size: 6415 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6415 XmaI C'CCGG_G Cuts at: 1870 3740 1870 Size: 1870 4545 XmnI GAAnn'nnTTC Cuts at: 2397 4544 2397 Size: 2147 4268 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4223 276 Size: 53 83 186 936 2689 2468 Fragments arranged by size: 2689 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI