################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII AccIII AcyI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BclI BcnI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmFI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI Ksp632I MamI MfeI MluI MroI MspI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 901 GTAGAC GT^[AC][GT]AC AccII 837 CGCG CG^CG AclI 644 AACGTT AA^CGTT AflII 150 CTTAAG C^TTAAG AlwI 216 GGATC GGATC(4/5) AspHI 327 GAGCAC G[AT]GC[AT]^C AvaI 443 CTCGAG C^[CT]CG[GA]G BbsI 132 GAAGAC GAAGAC(2/6) BciVI 680 GTATCC GTATCC(6/5) BfaI 472 CTAG C^TAG BfrI 150 CTTAAG C^TTAAG BmyI 327 GAGCAC G[AGT]GC[ACT]^C BpuAI 132 GAAGAC GAAGAC(2/6) BsaI 725 GGTCTC GGTCTC(1/5) BsiHKAI 327 GAGCAC G[AT]GC[AT]^C BsmBI 880 GAGACG CGTCTC(1/5) BsoBI 443 CTCGAG C^[CT]CG[GA]G Bsp1286I 327 GAGCAC G[AGT]GC[ACT]^C BsrDI 109 CATTGC GCAATG(2/0) BsrI 97 CCAGT ACTGG(1/-1) BstAPI 329 GCACAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 837 CGCG CG^CG BstYI 216 GGATCT [GA]^GATC[CT] DpnII 217 GATC ^GATC DraI 778 TTTAAA TTT^AAA DraII 10 AGGACCC [GA]G^G[ACGT]CC[CT] EcoNI 66 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 10 AGGACCC [GA]G^G[ACGT]CC[CT] HgaI 359 GCGTC GACGC(5/10) KspI 836 CCGCGG CCGC^GG MaeI 472 CTAG C^TAG MboI 217 GATC ^GATC MflI 216 GGATCT [GA]^GATC[CT] MspA1I 836 CCGCGG C[AC]G^C[GT]G MvnI 837 CGCG CG^CG NcoI 848 CCATGG C^CATGG NdeII 217 GATC ^GATC PaeR7I 443 CTCGAG C^TCGAG PleI 313 GACTC GAGTC(4/5) PpuMI 10 AGGACCC [GA]G^G[AT]CC[CT] Psp1406I 644 AACGTT AA^CGTT SacII 836 CCGCGG CCGC^GG Sau3AI 217 GATC ^GATC StuI 342 AGGCCT AGG^CCT TaqI 444 TCGA T^CGA Tsp45I 361 GTCAC ^GT[GC]AC TspRI 17 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 444 TCGA T^CGA XcmI 684 CCAATATAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 443 CTCGAG C^TCGAG XhoII 216 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### Acc65I 117 GGTACC G^GTACC 592 GGTACC AcsI 520 AAATTC [GA]^AATT[CT] 691 AAATTT ApoI 520 AAATTC [GA]^AATT[CT] 691 AAATTT Asp718I 117 GGTACC G^GTACC 592 GGTACC BalI 275 TGGCCA TGG^CCA 851 TGGCCA BanI 117 GGTACC G^G[CT][GA]CC 592 GGTACC BsmAI 880 GAGAC GTCTC(1/5) 726 GTCTC BsmI 303 GAATGC GAATGC(1/-1) 355 GAATGC BstXI 147 CCACTTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 186 CCATCCTGATGG Cac8I 201 GCTTGC GC[ACGT]^[ACGT]GC 235 GCTTGC DdeI 794 CTGAG C^T[ACGT]AG 864 CTCAG DsaI 836 CCGCGG C^C[GA][CT]GG 848 CCATGG EcoT14I 501 CCTTGG C^C[AT][AT]GG 848 CCATGG FauI 143 CCCGC CCCGC(4/6) 838 GCGGG KpnI 117 GGTACC GGTAC^C 592 GGTACC MaeII 577 ACGT A^CGT 645 ACGT MluNI 275 TGGCCA TGG^CCA 851 TGGCCA MscI 275 TGGCCA TGG^CCA 851 TGGCCA NlaIII 179 CATG CATG^ 849 CATG PstI 67 CTGCAG CTGCA^G 461 CTGCAG SfaNI 420 GCATC GCATC(5/9) 554 GCATC StyI 501 CCTTGG C^C[AT][AT]GG 848 CCATGG Tsp509I 521 AATT ^AATT 692 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AfaI AluI AvaII BbvI BpmI BsaJI BsiYI BslI BspMI BssKI BstF5I BstNI Cfr13I EaeI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboII MnlI MseI MslI MvaI MwoI NlaIV RsaI Sau96I ScrFI SfcI SmlI TfiI Tru9I TseI