################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BfaI BfrI BlnI BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I Fnu4HI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI ItaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tru9I TseI Tsp45I TthHB8I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 328 GTATAC GT^[AC][GT]AC AcsI 445 AAATTC [GA]^AATT[CT] AlwI 208 GATCC GGATC(4/5) AocI 226 CCTAAGG CC^T[ACGT]AGG ApoI 445 AAATTC [GA]^AATT[CT] AspHI 601 GTGCTC G[AT]GC[AT]^C AspI 625 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BclI 558 TGATCA T^GATCA BglI 161 GCCGTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 401 AGATCT A^GATCT BlpI 335 GCTCAGC GC^T[ACGT]AGC Bpu1102I 335 GCTCAGC GC^T[ACGT]AGC BsaWI 247 ACCGGA [AT]^CCGG[AT] BsgI 105 CTGCAC GTGCAG(16/14) BsiHKAI 601 GTGCTC G[AT]GC[AT]^C BsmFI 269 GTCCC GGGAC(10/14) BsmI 340 GCATTC GAATGC(1/-1) BspHI 45 TCATGA T^CATGA BsrBI 333 CCGCTC CCGCTC(-3/-3) BsrI 439 ACTGG ACTGG(1/-1) Bst1107I 328 GTATAC GTA^TAC BstZ17I 328 GTATAC GTA^TAC Bsu36I 226 CCTAAGG CC^T[ACGT]AGG CelII 335 GCTCAGC GC^T[ACGT]AGC Eco81I 226 CCTAAGG CC^T[ACGT]AGG FauI 121 GCGGG CCCGC(4/6) FbaI 558 TGATCA T^GATCA MaeII 365 ACGT A^CGT MaeIII 367 GTTAC ^GT[ACGT]AC MslI 424 CACTATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 380 ACATGC [GA]CATG^[CT] PflFI 625 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 267 AGGTCCC [GA]G^G[AT]CC[CT] RcaI 45 TCATGA T^CATGA SapI 546 GCTCTTC GCTCTTC(1/4) SmaI 256 CCCGGG CCC^GGG SmlI 65 CTCAAG C^T[CT][GA]AG StuI 587 AGGCCT AGG^CCT TfiI 287 GAATC G^A[AT]TC Tsp509I 446 AATT ^AATT Tth111I 625 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 256 CCCGGG C^CCGGG XmaI 256 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 333 CCGC CCGC(-3/-1) 121 GCGG AluI 15 AGCT AG^CT 33 AGCT ApaI 70 GGGCCC GGGCC^C 152 GGGCCC AvaI 256 CCCGGG C^[CT]CG[GA]G 272 CCCGAG BanI 178 GGCACC G^G[CT][GA]CC 319 GGTGCC BanII 70 GGGCCC G[GA]GC[CT]^C 152 GGGCCC BsmAI 233 GAGAC GTCTC(1/5) 358 GAGAC BsoBI 256 CCCGGG C^[CT]CG[GA]G 272 CCCGAG BstXI 423 CCACTATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 639 CCAGGTGTATGG BstYI 207 AGATCC [GA]^GATC[CT] 401 AGATCT Cac8I 103 GCCTGC GC[ACGT]^[ACGT]GC 121 GCGGGC DdeI 227 CTAAG C^T[ACGT]AG 336 CTCAG EarI 523 CTCTTC CTCTTC(1/4) 547 CTCTTC EcoNI 90 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 310 CCTGGAGAAGG EcoT14I 95 CCAAGG C^C[AT][AT]GG 291 CCAAGG Ksp632I 523 CTCTTC CTCTTC(1/4) 547 CTCTTC MflI 207 AGATCC [GA]^GATC[CT] 401 AGATCT StyI 95 CCAAGG C^C[AT][AT]GG 291 CCAAGG XhoII 207 AGATCC [GA]^GATC[CT] 401 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AfaI AvaII BcnI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cfr13I DpnII DraII EcoO109I EcoRII FokI HaeIII HapII HinfI HpaII HphI MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TspRI