(Circular) (Six-base) MAPSORT of: pFN21AE1740.seq Check: 9749 from: 1 to: 5437 pFN21AE1740 5437 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3247 278 Size: 53 83 186 936 1711 2468 Fragments arranged by size: 2468 1711 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2334 2780 1937 Size: 397 446 4594 Fragments arranged by size: 4594 446 397 Acc65I G'GTAC_C Cuts at: 2758 2758 Size: 5437 AclI AA'CG_TT Cuts at: 1563 2736 3564 3937 1563 Size: 1173 828 373 3063 Fragments arranged by size: 3063 1173 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2289 3500 4884 5152 1632 Size: 657 1211 1384 268 1917 Fragments arranged by size: 1917 1384 1211 657 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5240 AflIII A'CryG_T Cuts at: 1203 4336 5313 1203 Size: 3133 977 1327 Fragments arranged by size: 3133 1327 977 AgeI A'CCGG_T Cuts at: 4268 4268 Size: 5437 AhdI GACnn_n'nnGTC Cuts at: 4166 4166 Size: 5437 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5437 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5437 AlwNI CAG_nnn'CTG Cuts at: 3180 4752 3180 Size: 1572 3865 ApaI G_GGCC'C Cuts at: 2079 2161 2079 Size: 82 5355 ApaLI G'TGCA_C Cuts at: 3494 4650 3494 Size: 1156 4281 ApoI r'AATT_y Cuts at: 1493 2450 2746 2972 3124 5233 1493 Size: 957 296 226 152 2109 1697 Fragments arranged by size: 2109 1697 957 296 226 152 AseI AT'TA_AT Cuts at: 160 1032 3991 160 Size: 872 2959 1606 Fragments arranged by size: 2959 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 AvaI C'yCGr_G Cuts at: 1870 1943 2261 2277 2762 1870 Size: 73 318 16 485 4545 Fragments arranged by size: 4545 485 318 73 16 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2241 2274 2241 Size: 33 5404 BamHI G'GATC_C Cuts at: 2767 3134 2767 Size: 367 5070 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2183 2324 2758 4213 618 Size: 325 217 627 396 141 434 1455 1842 Fragments arranged by size: 1842 1455 627 434 396 325 217 141 BanII G_rGCy'C Cuts at: 1391 2079 2161 2756 1391 Size: 688 82 595 4072 Fragments arranged by size: 4072 688 595 82 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4466 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3628 3662 1903 Size: 34 50 34 1607 34 3678 Fragments arranged by size: 3678 1607 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3329 4539 3329 Size: 1210 4227 BclI T'GATC_A Cuts at: 1548 2563 1548 Size: 1015 4422 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2172 4048 136 Size: 107 122 71 631 605 500 1876 1525 Fragments arranged by size: 1876 1525 631 605 500 122 107 71 BglII A'GATC_T Cuts at: 2406 5432 2406 Size: 3026 2411 BlpI GC'TnA_GC Cuts at: 2341 2854 2341 Size: 513 4924 Bme1580I G_kGCm'C Cuts at: 1790 2079 2161 3498 4654 1790 Size: 289 82 1337 1156 2573 Fragments arranged by size: 2573 1337 1156 289 82 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5437 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3168 4126 442 Size: 416 53 83 399 24 1751 958 1753 Fragments arranged by size: 1753 1751 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5437 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2337 2627 2639 4097 1239 Size: 1098 290 12 1458 2579 Fragments arranged by size: 2579 1458 1098 290 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2055 3562 4427 4725 2055 Size: 1507 865 298 2767 Fragments arranged by size: 2767 1507 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4100 882 Size: 617 2601 2219 Fragments arranged by size: 2601 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3228 493 Size: 711 2024 2702 Fragments arranged by size: 2702 2024 711 BsaBI GATnn'nnATC Cuts at: 5431 5431 Size: 5437 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3244 3626 4261 275 Size: 53 83 186 936 1711 382 635 1451 Fragments arranged by size: 1711 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2252 3870 4268 4542 4689 5100 2252 Size: 1618 398 274 147 411 2589 Fragments arranged by size: 2589 1618 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2579 2609 2681 2711 1178 Size: 30 165 30 1176 30 72 30 3904 Fragments arranged by size: 3904 1176 165 72 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5437 BseYI C'CCAG_C Cuts at: 2014 4640 5136 2014 Size: 2626 496 2315 Fragments arranged by size: 2626 2315 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2095 1163 Size: 573 359 4505 Fragments arranged by size: 4505 573 359 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2887 3648 4676 1540 Size: 275 186 886 761 1028 2301 Fragments arranged by size: 2301 1028 886 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2610 3498 3583 4654 2610 Size: 888 85 1071 3393 Fragments arranged by size: 3393 1071 888 85 BsmI GAATG_Cn' Cuts at: 2345 2948 3041 2345 Size: 603 93 4741 Fragments arranged by size: 4741 603 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2161 2610 2756 3498 3583 4654 Size: 399 289 82 449 146 742 85 1071 Cuts at: 4654 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 449 399 289 146 85 82 BspEI T'CCGG_A Cuts at: 5100 5100 Size: 5437 BspHI T'CATG_A Cuts at: 2050 3326 5056 2050 Size: 1276 1730 2431 Fragments arranged by size: 2431 1730 1276 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2792 844 Size: 1948 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2340 3324 1394 Size: 429 517 984 3507 Fragments arranged by size: 3507 984 517 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3932 4106 65 Size: 1183 2684 174 1396 Fragments arranged by size: 2684 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4081 4268 1700 Size: 110 2271 187 2869 Fragments arranged by size: 2869 2271 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5437 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5437 BssSI C'ACGA_G Cuts at: 3497 4509 3497 Size: 1012 4425 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5437 BstBI TT'CG_AA Cuts at: 4241 4241 Size: 5437 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5437 BstXI CCAn_nnnn'nTGG Cuts at: 2435 2651 2435 Size: 216 5221 BstYI r'GATC_y Cuts at: 1972 2212 2406 2767 3134 3519 3536 4988 5074 Size: 240 194 361 367 385 17 1452 86 Cuts at: 5074 5086 5432 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 361 346 240 194 86 17 12 BstZ17I GTA'TAC Cuts at: 2335 2335 Size: 5437 Bsu36I CC'TnA_GG Cuts at: 2232 2232 Size: 5437 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4885 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4698 Fragments arranged by size: 4698 683 56 BtsI GCAGTG_nn' Cuts at: 2455 2953 3747 3767 2455 Size: 498 794 20 4125 Fragments arranged by size: 4125 794 498 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5437 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5402 DraI TTT'AAA Cuts at: 2741 2741 Size: 5437 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4444 809 Size: 507 3128 1802 Fragments arranged by size: 3128 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2884 3773 5402 Size: 54 1425 50 275 18 1054 889 1629 Cuts at: 5402 8 Size: 43 Fragments arranged by size: 1629 1425 1054 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2884 1537 Size: 275 1072 4090 Fragments arranged by size: 4090 1072 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2534 2558 3367 1353 Size: 1181 24 809 3423 Fragments arranged by size: 3423 1181 809 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4024 4398 4544 387 Size: 1064 138 2435 374 146 1280 Fragments arranged by size: 2435 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2289 2337 2627 2639 3500 4097 4884 Size: 393 657 48 290 12 861 597 787 Cuts at: 4884 5152 1239 Size: 268 1524 Fragments arranged by size: 1524 861 787 657 597 393 290 268 48 12 EcoNI CCTnn'n_nnAGG Cuts at: 2099 2319 2099 Size: 220 5217 EcoO109I rG'GnC_Cy Cuts at: 2075 2106 2157 2158 2273 2075 Size: 31 51 1 115 5239 Fragments arranged by size: 5239 115 51 31 1 EcoRI G'AATT_C Cuts at: 2746 3124 2746 Size: 378 5059 FspI TGC'GCA Cuts at: 3943 3943 Size: 5437 HaeII r_GCGC'y Cuts at: 1678 4584 1678 Size: 2906 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2579 2611 2681 2713 4078 Size: 32 135 32 1007 32 70 32 1365 Cuts at: 4078 4110 4152 4184 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1365 1007 135 70 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2781 3027 669 Size: 988 281 843 246 3079 Fragments arranged by size: 3079 988 843 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5437 HpaI GTT'AAC Cuts at: 3027 3027 Size: 5437 KpnI G_GTAC'C Cuts at: 2762 2762 Size: 5437 MfeI C'AATT_G Cuts at: 3036 3036 Size: 5437 MluI A'CGCG_T Cuts at: 5313 5313 Size: 5437 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2099 4551 4735 1305 Size: 794 2452 184 2007 Fragments arranged by size: 2452 2007 794 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3615 Fragments arranged by size: 3615 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2433 3395 3754 3913 518 Size: 648 1267 962 359 159 2042 Fragments arranged by size: 2042 1267 962 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2853 3530 4678 4923 1606 Size: 1247 677 1148 245 2120 Fragments arranged by size: 2120 1247 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4885 NdeI CA'TA_TG Cuts at: 387 387 Size: 5437 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5437 NotI GC'GGCC_GC Cuts at: 2884 2884 Size: 5437 NspI r_CATG'y Cuts at: 1132 1229 2389 2795 4340 1132 Size: 97 1160 406 1545 2229 Fragments arranged by size: 2229 1545 1160 406 97 PasI CC'CwG_GG Cuts at: 1348 2135 1348 Size: 787 4650 PciI A'CATG_T Cuts at: 4336 4336 Size: 5437 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5437 PfoI T'CCnGG_A Cuts at: 2287 2287 Size: 5437 PmeI GTTT'AAAC Cuts at: 2741 2741 Size: 5437 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3506 3538 5019 5051 3506 Size: 32 1481 32 3892 Fragments arranged by size: 3892 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2273 2273 Size: 5437 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5437 PsiI TTA'TAA Cuts at: 3007 3007 Size: 5437 PspOMI G'GGCC_C Cuts at: 2075 2157 2075 Size: 82 5355 PstI C_TGCA'G Cuts at: 830 2789 830 Size: 1959 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5437 SalI G'TCGA_C Cuts at: 1936 2779 1936 Size: 843 4594 SapI GCTCTTCn'nnn_ Cuts at: 2558 2558 Size: 5437 SbfI CC_TGCA'GG Cuts at: 2789 2789 Size: 5437 ScaI AGT'ACT Cuts at: 3685 5397 3685 Size: 1712 3725 SfcI C'TryA_G Cuts at: 826 1045 2785 3920 4601 4792 826 Size: 219 1740 1135 681 191 1471 Fragments arranged by size: 1740 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5437 SmaI CCC'GGG Cuts at: 1872 2263 2764 1872 Size: 391 501 4545 Fragments arranged by size: 4545 501 391 SmlI C'TyrA_G Cuts at: 820 1017 1943 2070 3541 4442 4704 820 Size: 197 926 127 1471 901 262 1553 Fragments arranged by size: 1553 1471 926 901 262 197 127 SnaBI TAC'GTA Cuts at: 493 3228 493 Size: 2735 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5437 SphI G_CATG'C Cuts at: 1132 2795 1132 Size: 1663 3774 SspI AAT'ATT Cuts at: 5 52 3361 5 Size: 47 3309 2081 Fragments arranged by size: 3309 2081 47 StuI AGG'CCT Cuts at: 1517 2594 1517 Size: 1077 4360 StyI C'CwwG_G Cuts at: 513 1065 2100 2296 2876 513 Size: 552 1035 196 580 3074 Fragments arranged by size: 3074 1035 580 552 196 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3464 3481 3634 3819 4264 325 Size: 1255 1884 17 153 185 445 1498 Fragments arranged by size: 1884 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2475 3683 5395 Size: 274 80 33 51 1443 498 1208 1712 Cuts at: 5395 96 Size: 138 Fragments arranged by size: 1712 1443 1208 498 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5397 5429 5397 Size: 32 5405 Tth111I GACn'n_nGTC Cuts at: 1316 2633 1316 Size: 1317 4120 XbaI T'CTAG_A Cuts at: 2773 2773 Size: 5437 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5437 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5437 XmaI C'CCGG_G Cuts at: 1870 2261 2762 1870 Size: 391 501 4545 Fragments arranged by size: 4545 501 391 XmnI GAAnn'nnTTC Cuts at: 3566 3566 Size: 5437 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3245 276 Size: 53 83 186 936 1711 2468 Fragments arranged by size: 2468 1711 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI