################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII BalI BanI BbeI BbrPI BbsI BcgI BclI BfrI BglI BglII BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstPI BstUI CelII CfoI Cfr10I ClaI CpoI DraI DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 909 GTATAC GT^[AC][GT]AC AciI 522 GCGG CCGC(-3/-1) AlwNI 1039 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 824 CCTCAGG CC^T[ACGT]AGG AspHI 580 GAGCTC G[AT]GC[AT]^C AvrII 804 CCTAGG C^CTAGG BamHI 429 GGATCC G^GATCC BbvCI 626 CCTCAGC CCTCAGC(-5/-2) BlnI 804 CCTAGG C^CTAGG BsaI 232 GAGACC GGTCTC(1/5) BsiHKAI 580 GAGCTC G[AT]GC[AT]^C BsmBI 355 CGTCTC CGTCTC(1/5) BspMI 461 ACCTGC ACCTGC(4/8) Bst1107I 909 GTATAC GTA^TAC BstYI 429 GGATCC [GA]^GATC[CT] BstZ17I 909 GTATAC GTA^TAC Bsu36I 824 CCTCAGG CC^T[ACGT]AGG DpnII 430 GATC ^GATC Eco81I 824 CCTCAGG CC^T[ACGT]AGG EcoNI 791 CCTCACGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 522 GCGGG CCCGC(4/6) HgaI 515 GCGTC GACGC(5/10) HphI 159 TCACC GGTGA(8/7) MboI 430 GATC ^GATC MflI 429 GGATCC [GA]^GATC[CT] MslI 942 CACCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 539 CAGCTG C[AC]G^C[GT]G NdeII 430 GATC ^GATC PstI 373 CTGCAG CTGCA^G PvuII 539 CAGCTG CAG^CTG SacI 580 GAGCTC GAGCT^C SapI 915 GCTCTTC GCTCTTC(1/4) Sau3AI 430 GATC ^GATC SfaNI 489 GATGC GCATC(5/9) SfcI 373 CTGCAG C^T[GA][CT]AG Tsp509I 14 AATT ^AATT XcmI 891 CCATCCTCCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 429 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AlwI 430 GATCC GGATC(4/5) 429 GGATC ApaI 368 GGGCCC GGGCC^C 524 GGGCCC BciVI 733 GGATAC GTATCC(6/5) 746 GGATAC BfaI 562 CTAG C^TAG 805 CTAG BssSI 22 CTCGTG CACGAG(-5/-1) 770 CTCGTG BstXI 710 CCAGGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 941 CCACCCCTGTGG Cac8I 186 GCCTGC GC[ACGT]^[ACGT]GC 522 GCGGGC HaeIII 369 GGCC GG^CC 525 GGCC MaeI 562 CTAG C^TAG 805 CTAG MwoI 70 GCTCCTCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 134 GCAGACTGAGC NlaIII 30 CATG CATG^ 121 CATG ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AluI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DraII DrdI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HapII HinfI HpaII ItaI Ksp632I MaeIII MboII MnlI MspI MvaI NciI NlaIV PleI PpuMI Sau96I ScrFI StyI TfiI TseI Tsp45I TspRI