################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII AccI AccIII AciI AclI AcyI AflII AgeI AhdI AluI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BcgI BclI BcnI BfaI BfrI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI Ksp632I KspI MaeI MamI MfeI MluNI MroI MscI MspA1I MspI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 101 GGTACC G^GTACC AccII 136 CGCG CG^CG AcsI 822 GAATTT [GA]^AATT[CT] AlwNI 536 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 822 GAATTT [GA]^AATT[CT] Asp718I 101 GGTACC G^GTACC BbvCI 105 CCTCAGC CCTCAGC(-5/-2) BciVI 684 GTATCC GTATCC(6/5) BglI 277 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsiYI 50 CCCTCAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 50 CCCTCAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 906 GAGAC GTCTC(1/5) BsmBI 906 GAGACG CGTCTC(1/5) BspMI 960 GCAGGT ACCTGC(4/8) BstAPI 960 GCAGGTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 249 GGTGACC G^GT[ACGT]ACC BstPI 249 GGTGACC G^GT[ACGT]ACC BstUI 136 CGCG CG^CG BstXI 737 CCAGAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 282 GCAGGC GC[ACGT]^[ACGT]GC DraI 582 TTTAAA TTT^AAA DsaI 924 CCATGG C^C[GA][CT]GG EcoO65I 249 GGTGACC G^GT[ACGT]ACC HincII 899 GTCAAC GT[CT]^[GA]AC HindII 899 GTCAAC GT[CT]^[GA]AC HinfI 881 GAATC G^A[ACGT]TC KpnI 101 GGTACC GGTAC^C MluI 135 ACGCGT A^CGCGT MvnI 136 CGCG CG^CG NcoI 924 CCATGG C^CATGG NspI 982 ACATGC [GA]CATG^[CT] PstI 711 CTGCAG CTGCA^G SnaBI 523 TACGTA TAC^GTA TaqI 201 TCGA T^CGA TfiI 881 GAATC G^A[AT]TC TthHB8I 201 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflIII 135 ACGCGT A^C[GA][CT]GT 909 ACGTGT Asp700I 406 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 603 GAAGTTTTTC BanI 101 GGTACC G^G[CT][GA]CC 747 GGTGCC BbvI 919 GCAGC GCAGC(8/12) 69 GCTGC BsaAI 523 TACGTA [CT]AC^GT[GA] 775 CACGTA BsmI 207 GCATTC GAATGC(1/-1) 484 GCATTC BsrI 143 ACTGG ACTGG(1/-1) 559 ACTGG BssKI 278 CCAGG ^CC[ACGT]GG 678 CCAGG BstNI 278 CCAGG CC^[AT]GG 678 CCAGG EcoRII 278 CCAGG ^CC[AT]GG 678 CCAGG Fnu4HI 69 GCTGC GC^[ACGT]GC 919 GCAGC ItaI 69 GCTGC GC^[ACGT]GC 919 GCAGC MseI 583 TTAA T^TAA 829 TTAA MvaI 278 CCAGG CC^[AT]GG 678 CCAGG MwoI 277 GCCAGGCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 960 GCAGGTCATGC ScrFI 278 CCAGG CC^[ACGT]GG 678 CCAGG SfcI 642 CTATAG C^T[GA][CT]AG 711 CTGCAG Tru9I 583 TTAA T^TAA 829 TTAA TseI 69 GCTGC G^C[AT]GC 919 GCAGC XmnI 406 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 603 GAAGTTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AfaI AlwI BbsI BpuAI BsaJI BstF5I BstYI DdeI DpnII EcoT14I FokI HaeIII HphI MaeII MaeIII MboI MboII MflI MnlI MslI NdeII NlaIII NlaIV RsaI Sau3AI StyI Tsp45I Tsp509I TspRI XhoII