(Circular) (Six-base) MAPSORT of: pFN21AE6621.seq Check: 980 from: 1 to: 5698 pFN21AE6621 5698 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3508 278 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3041 1937 Size: 1104 4594 Acc65I G'GTAC_C Cuts at: 2106 3019 2106 Size: 913 4785 AclI AA'CG_TT Cuts at: 1563 3825 4198 1563 Size: 2262 373 3063 Fragments arranged by size: 3063 2262 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2253 3761 5145 5413 1632 Size: 621 1508 1384 268 1917 Fragments arranged by size: 1917 1508 1384 621 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5501 AflIII A'CryG_T Cuts at: 1203 2140 2914 4597 5574 1203 Size: 937 774 1683 977 1327 Fragments arranged by size: 1683 1327 977 937 774 AgeI A'CCGG_T Cuts at: 4529 4529 Size: 5698 AhdI GACnn_n'nnGTC Cuts at: 4427 4427 Size: 5698 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5698 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5698 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2322 2354 2322 Size: 32 5666 AlwNI CAG_nnn'CTG Cuts at: 2546 3441 5013 2546 Size: 895 1572 3231 Fragments arranged by size: 3231 1572 895 ApaLI G'TGCA_C Cuts at: 3755 4911 3755 Size: 1156 4542 ApoI r'AATT_y Cuts at: 1493 2827 3007 3233 3385 5494 1493 Size: 1334 180 226 152 2109 1697 Fragments arranged by size: 2109 1697 1334 226 180 152 AseI AT'TA_AT Cuts at: 160 1032 4252 160 Size: 872 3220 1606 Fragments arranged by size: 3220 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 AvaI C'yCGr_G Cuts at: 1870 1943 3023 1870 Size: 73 1080 4545 Fragments arranged by size: 4545 1080 73 BamHI G'GATC_C Cuts at: 3028 3395 3028 Size: 367 5331 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2106 2752 3019 4474 618 Size: 325 217 627 319 646 267 1455 1842 Fragments arranged by size: 1842 1455 646 627 325 319 267 217 BanII G_rGCy'C Cuts at: 1391 3017 1391 Size: 1626 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2385 2543 2865 928 Size: 971 486 158 322 3761 Fragments arranged by size: 3761 971 486 322 158 BbvCI CC'TCA_GC Cuts at: 2111 2111 Size: 5698 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3889 3923 1903 Size: 34 1952 34 3678 Fragments arranged by size: 3678 1952 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2700 3590 4800 2700 Size: 890 1210 3598 Fragments arranged by size: 3598 1210 890 BclI T'GATC_A Cuts at: 1548 1548 Size: 5698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2288 4309 136 Size: 107 122 71 631 605 616 2021 1525 Fragments arranged by size: 2021 1525 631 616 605 122 107 71 BglII A'GATC_T Cuts at: 5693 5693 Size: 5698 BlpI GC'TnA_GC Cuts at: 3115 3115 Size: 5698 Bme1580I G_kGCm'C Cuts at: 1790 3759 4915 1790 Size: 1969 1156 2573 Fragments arranged by size: 2573 1969 1156 BmgBI CAC'GTC Cuts at: 1318 2915 1318 Size: 1597 4101 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3429 4387 442 Size: 416 53 83 399 24 2012 958 1753 Fragments arranged by size: 2012 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5698 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4358 1239 Size: 3119 2579 Bpu10I CC'TnA_GC Cuts at: 2111 2975 2111 Size: 864 4834 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3823 4688 4986 3823 Size: 865 298 4535 Fragments arranged by size: 4535 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4361 882 Size: 617 2862 2219 Fragments arranged by size: 2862 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2530 2782 3489 493 Size: 711 1326 252 707 2702 Fragments arranged by size: 2702 1326 711 707 252 BsaBI GATnn'nnATC Cuts at: 5692 5692 Size: 5698 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3505 3887 4522 275 Size: 53 83 186 936 1972 382 635 1451 Fragments arranged by size: 1972 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4131 4529 4803 4950 5361 4131 Size: 398 274 147 411 4468 Fragments arranged by size: 4468 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5473 Fragments arranged by size: 5473 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5698 BseYI C'CCAG_C Cuts at: 4901 5397 4901 Size: 496 5202 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5125 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3148 3909 4937 1540 Size: 275 186 1147 761 1028 2301 Fragments arranged by size: 2301 1147 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3759 3844 4915 3759 Size: 85 1071 4542 Fragments arranged by size: 4542 1071 85 BsmI GAATG_Cn' Cuts at: 2212 2489 3209 3302 2212 Size: 277 720 93 4608 Fragments arranged by size: 4608 720 277 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2905 2905 Size: 5698 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3017 3759 3844 4915 1391 Size: 399 1227 742 85 1071 2174 Fragments arranged by size: 2174 1227 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5361 5361 Size: 5698 BspHI T'CATG_A Cuts at: 3587 5317 3587 Size: 1730 3968 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2956 3053 844 Size: 2112 97 3489 Fragments arranged by size: 3489 2112 97 BsrBI CCG'CTC Cuts at: 1394 1823 3585 1394 Size: 429 1762 3507 Fragments arranged by size: 3507 1762 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4193 4367 65 Size: 1183 2945 174 1396 Fragments arranged by size: 2945 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4342 4529 1700 Size: 110 2532 187 2869 Fragments arranged by size: 2869 2532 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5698 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5698 BssSI C'ACGA_G Cuts at: 3758 4770 3758 Size: 1012 4686 BstAPI GCAn_nnn'nTGC Cuts at: 794 2971 794 Size: 2177 3521 BstBI TT'CG_AA Cuts at: 4502 4502 Size: 5698 BstEII G'GTnAC_C Cuts at: 1184 2254 1184 Size: 1070 4628 BstXI CCAn_nnnn'nTGG Cuts at: 2749 2749 Size: 5698 BstYI r'GATC_y Cuts at: 1972 2051 2760 2817 3028 3395 3780 3797 5249 Size: 79 709 57 211 367 385 17 1452 Cuts at: 5249 5335 5347 5693 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 709 385 367 346 211 86 79 57 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2929 513 Size: 552 939 925 3282 Fragments arranged by size: 3282 939 925 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4959 Fragments arranged by size: 4959 683 56 BtsI GCAGTG_nn' Cuts at: 2725 3214 4008 4028 2725 Size: 489 794 20 4395 Fragments arranged by size: 4395 794 489 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5698 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5663 DraI TTT'AAA Cuts at: 2589 3002 2589 Size: 413 5285 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4705 809 Size: 507 3389 1802 Fragments arranged by size: 3389 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3145 4034 5663 Size: 54 1425 50 275 18 1315 889 1629 Cuts at: 5663 8 Size: 43 Fragments arranged by size: 1629 1425 1315 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3145 1537 Size: 275 1333 4090 Fragments arranged by size: 4090 1333 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3628 1353 Size: 2275 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4285 4659 4805 387 Size: 1064 138 2696 374 146 1280 Fragments arranged by size: 2696 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2253 3761 4358 5145 5413 1239 Size: 393 621 1508 597 787 268 1524 Fragments arranged by size: 1524 1508 787 621 597 393 268 EcoRI G'AATT_C Cuts at: 3007 3385 3007 Size: 378 5320 FspI TGC'GCA Cuts at: 4204 4204 Size: 5698 HaeII r_GCGC'y Cuts at: 1678 4845 1678 Size: 3167 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4339 4371 4413 4445 1373 Size: 32 135 32 2767 32 42 32 2626 Fragments arranged by size: 2767 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2906 3042 3288 669 Size: 988 281 968 136 246 3079 Fragments arranged by size: 3079 988 968 281 246 136 HindIII A'AGCT_T Cuts at: 748 748 Size: 5698 HpaI GTT'AAC Cuts at: 3288 3288 Size: 5698 KpnI G_GTAC'C Cuts at: 2110 3023 2110 Size: 913 4785 MfeI C'AATT_G Cuts at: 3297 3297 Size: 5698 MluI A'CGCG_T Cuts at: 2140 5574 2140 Size: 3434 2264 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2461 4812 4996 1305 Size: 1156 2351 184 2007 Fragments arranged by size: 2351 2007 1156 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3876 Fragments arranged by size: 3876 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2127 2252 2494 2895 3656 4015 4174 Size: 648 961 125 242 401 761 359 159 Cuts at: 4174 518 Size: 2042 Fragments arranged by size: 2042 961 761 648 401 359 242 159 125 MspA1I CmG'CkG Cuts at: 1606 3114 3791 4939 5184 1606 Size: 1508 677 1148 245 2120 Fragments arranged by size: 2120 1508 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2929 513 Size: 552 939 925 3282 Fragments arranged by size: 3282 939 925 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5698 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5698 NotI GC'GGCC_GC Cuts at: 3145 3145 Size: 5698 NspI r_CATG'y Cuts at: 1132 1229 2991 3056 4601 1132 Size: 97 1762 65 1545 2229 Fragments arranged by size: 2229 1762 1545 97 65 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5698 PciI A'CATG_T Cuts at: 4597 4597 Size: 5698 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5698 PmeI GTTT'AAAC Cuts at: 3002 3002 Size: 5698 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3767 3799 5280 5312 3767 Size: 32 1481 32 4153 Fragments arranged by size: 4153 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5698 PsiI TTA'TAA Cuts at: 3268 3268 Size: 5698 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2138 2170 2138 Size: 32 5666 PstI C_TGCA'G Cuts at: 830 2720 3050 830 Size: 1890 330 3478 Fragments arranged by size: 3478 1890 330 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5698 SalI G'TCGA_C Cuts at: 1936 3040 1936 Size: 1104 4594 SbfI CC_TGCA'GG Cuts at: 3050 3050 Size: 5698 ScaI AGT'ACT Cuts at: 3946 5658 3946 Size: 1712 3986 SfcI C'TryA_G Cuts at: 826 1045 2647 2716 3046 4181 4862 5053 826 Size: 219 1602 69 330 1135 681 191 1471 Fragments arranged by size: 1602 1471 1135 681 330 219 191 69 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5698 SmaI CCC'GGG Cuts at: 1872 3025 1872 Size: 1153 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3802 4703 4965 820 Size: 197 926 1859 901 262 1553 Fragments arranged by size: 1859 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2530 3489 493 Size: 2037 959 2702 Fragments arranged by size: 2702 2037 959 SpeI A'CTAG_T Cuts at: 152 152 Size: 5698 SphI G_CATG'C Cuts at: 1132 3056 1132 Size: 1924 3774 SspI AAT'ATT Cuts at: 5 52 3622 5 Size: 47 3570 2081 Fragments arranged by size: 3570 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5698 StyI C'CwwG_G Cuts at: 513 1065 2004 2756 2929 2943 3137 513 Size: 552 939 752 173 14 194 3074 Fragments arranged by size: 3074 939 752 552 194 173 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3725 3742 3895 4080 4525 325 Size: 1255 2145 17 153 185 445 1498 Fragments arranged by size: 2145 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2154 3944 5656 Size: 274 80 33 51 1443 177 1790 1712 Cuts at: 5656 96 Size: 138 Fragments arranged by size: 1790 1712 1443 274 177 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2608 2640 5658 5690 2608 Size: 32 3018 32 2616 Fragments arranged by size: 3018 2616 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5698 XbaI T'CTAG_A Cuts at: 3034 3034 Size: 5698 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5698 XmaI C'CCGG_G Cuts at: 1870 3023 1870 Size: 1153 4545 XmnI GAAnn'nnTTC Cuts at: 2415 2612 3827 2415 Size: 197 1215 4286 Fragments arranged by size: 4286 1215 197 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3506 276 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI