Restriction map for pFN21AE6621
 (Linear) (Six-base) MAPSORT of: KIEE6621.seq  Check: 6857  from: 1  to: 993



KIEE6621.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

Acc65I G'GTAC_C

Cuts at:      0     101     993 
   Size:        101     892
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     248     993 
   Size:        248     745
 
AflIII A'CryG_T

Cuts at:      0     135     909     993 
   Size:        135     774      84

  Fragments arranged by size: 

                 774     135      84
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     317     349     993 
   Size:        317      32     644

  Fragments arranged by size: 

                 644     317      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     541     993 
   Size:        541     452
 
ApoI r'AATT_y

Cuts at:      0     822     993 
   Size:        822     171
 
BanI G'GyrC_C

Cuts at:      0     101     747     993 
   Size:        101     646     246

  Fragments arranged by size: 

                 646     246     101
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     380     538     860     993 
   Size:        380     158     322     133

  Fragments arranged by size: 

                 380     322     158     133
 
BbvCI CC'TCA_GC

Cuts at:      0     106     993 
   Size:        106     887
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     695     993 
   Size:        695     298
 
BglI GCCn_nnn'nGGC

Cuts at:      0     283     993 
   Size:        283     710
 
BmgBI CAC'GTC

Cuts at:      0     910     993 
   Size:        910      83
 
Bpu10I CC'TnA_GC

Cuts at:      0     106     970     993 
   Size:        106     864      23

  Fragments arranged by size: 

                 864     106      23
 
BsaAI yAC'GTr

Cuts at:      0     525     777     993 
   Size:        525     252     216

  Fragments arranged by size: 

                 525     252     216
 
BsmI GAATG_Cn'

Cuts at:      0     207     484     993 
   Size:        207     277     509

  Fragments arranged by size: 

                 509     277     207
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     900     993 
   Size:        900      93
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     951     993 
   Size:        951      42
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     966     993 
   Size:        966      27
 
BstEII G'GTnAC_C

Cuts at:      0     249     993 
   Size:        249     744
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     744     993 
   Size:        744     249
 
BstYI r'GATC_y

Cuts at:      0      46     755     812     993 
   Size:         46     709      57     181

  Fragments arranged by size: 

                 709     181      57      46
 
BtgI C'CryG_G

Cuts at:      0     924     993 
   Size:        924      69
 
BtsI GCAGTG_nn'

Cuts at:      0     720     993 
   Size:        720     273
 
DraI TTT'AAA

Cuts at:      0     584     993 
   Size:        584     409
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     248     993 
   Size:        248     745
 
HincII GTy'rAC

Cuts at:      0     901     993 
   Size:        901      92
 
KpnI G_GTAC'C

Cuts at:      0     105     993 
   Size:        105     888
 
MluI A'CGCG_T

Cuts at:      0     135     993 
   Size:        135     858
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     456     993 
   Size:        456     537
 
MslI CAynn'nnrTG

Cuts at:      0     122     247     489     890     993 
   Size:        122     125     242     401     103

  Fragments arranged by size: 

                 401     242     125     122     103
 
NcoI C'CATG_G

Cuts at:      0     924     993 
   Size:        924      69
 
NspI r_CATG'y

Cuts at:      0     986     993 
   Size:        986       7
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     133     165     993 
   Size:        133      32     828

  Fragments arranged by size: 

                 828     133      32
 
PstI C_TGCA'G

Cuts at:      0     715     993 
   Size:        715     278
 
SfcI C'TryA_G

Cuts at:      0     642     711     993 
   Size:        642      69     282

  Fragments arranged by size: 

                 642     282      69
 
SnaBI TAC'GTA

Cuts at:      0     525     993 
   Size:        525     468
 
StyI C'CwwG_G

Cuts at:      0     751     924     938     993 
   Size:        751     173      14      55

  Fragments arranged by size: 

                 751     173      55      14
 
TatI w'GTAC_w

Cuts at:      0     149     993 
   Size:        149     844
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     603     635     993 
   Size:        603      32     358

  Fragments arranged by size: 

                 603     358      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     410     607     993 
   Size:        410     197     386

  Fragments arranged by size: 

                 410     386     197
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AloI    AlwNI     ApoI     BanI     BbsI
    BbvCI    BciVI     BglI    BmgBI   Bpu10I    BsaAI     BsmI    BsmBI
    BspMI   BstAPI   BstEII    BstXI    BstYI     BtgI     BtsI     DraI
  Eco57MI   HincII     KpnI     MluI     MmeI     MslI     NcoI     NspI
     PsrI     PstI     SfcI    SnaBI     StyI     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII     BbeI     BcgI     BclI    BfrBI
    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     MfeI     MscI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI
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