################################## # NO CUT ENZYMES ( 225 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MseI MspA1I MspI MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AfaI 189 GTAC GT^AC AlwI 29 GGATC GGATC(4/5) AlwNI 140 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG BanI 133 GGCACC G^G[CT][GA]CC BbsI 61 GAAGAC GAAGAC(2/6) BbvI 69 GCTGC GCAGC(8/12) BpuAI 61 GAAGAC GAAGAC(2/6) BsiYI 208 CCCCCCATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 208 CCCCCCATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BstF5I 81 GGATG GGATG(2/0) BstYI 29 GGATCT [GA]^GATC[CT] DdeI 177 CTTAG C^T[ACGT]AG DpnII 30 GATC ^GATC Fnu4HI 69 GCTGC GC^[ACGT]GC FokI 81 GGATG GGATG(9/13) HinfI 142 GAATC G^A[ACGT]TC ItaI 69 GCTGC GC^[ACGT]GC MboI 30 GATC ^GATC MboII 61 GAAGA GAAGA(8/7) MflI 29 GGATCT [GA]^GATC[CT] MslI 196 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeII 30 GATC ^GATC NlaIII 196 CATG CATG^ NlaIV 133 GGCACC GG[ACGT]^[ACGT]CC RsaI 189 GTAC GT^AC Sau3AI 30 GATC ^GATC SfaNI 49 GATGC GCATC(5/9) TfiI 142 GAATC G^A[AT]TC TseI 69 GCTGC G^C[AT]GC XhoII 29 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 2 enzymes) # ################################### AluI 68 AGCT AG^CT 104 AGCT TspRI 6 CAGTG CA[GC]TG[ACGT][ACGT]^ 95 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 1 enzymes) # ######################################### MnlI