(Circular) (Six-base) MAPSORT of: pFN21AE3802.seq Check: 4644 from: 1 to: 4927 pFN21AE3802 4927 bp With 182 enzymes: SgfI * March 28, 2012 12:12 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2737 278 Size: 53 83 186 936 1201 2468 Fragments arranged by size: 2468 1201 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2270 1937 Size: 333 4594 Acc65I G'GTAC_C Cuts at: 2248 2248 Size: 4927 AclI AA'CG_TT Cuts at: 1563 2226 3054 3427 1563 Size: 663 828 373 3063 Fragments arranged by size: 3063 828 663 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2990 4374 4642 1632 Size: 1358 1384 268 1917 Fragments arranged by size: 1917 1384 1358 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4730 AflIII A'CryG_T Cuts at: 1203 3826 4803 1203 Size: 2623 977 1327 Fragments arranged by size: 2623 1327 977 AgeI A'CCGG_T Cuts at: 3758 3758 Size: 4927 AhdI GACnn_n'nnGTC Cuts at: 3656 3656 Size: 4927 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4927 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4927 AlwNI CAG_nnn'CTG Cuts at: 2150 2670 4242 2150 Size: 520 1572 2835 Fragments arranged by size: 2835 1572 520 ApaLI G'TGCA_C Cuts at: 2984 4140 2984 Size: 1156 3771 ApoI r'AATT_y Cuts at: 1493 2236 2462 2614 4723 1493 Size: 743 226 152 2109 1697 Fragments arranged by size: 2109 1697 743 226 152 AseI AT'TA_AT Cuts at: 160 1032 3481 160 Size: 872 2449 1606 Fragments arranged by size: 2449 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4927 AvaI C'yCGr_G Cuts at: 1870 1943 2252 1870 Size: 73 309 4545 Fragments arranged by size: 4545 309 73 BamHI G'GATC_C Cuts at: 2257 2624 2257 Size: 367 4560 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2138 2248 3703 618 Size: 325 217 627 351 110 1455 1842 Fragments arranged by size: 1842 1455 627 351 325 217 110 BanII G_rGCy'C Cuts at: 1391 2246 1391 Size: 855 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2073 928 Size: 971 174 3782 Fragments arranged by size: 3782 971 174 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3118 3152 1903 Size: 34 1181 34 3678 Fragments arranged by size: 3678 1181 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2819 4029 2819 Size: 1210 3717 BclI T'GATC_A Cuts at: 1548 1548 Size: 4927 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3538 136 Size: 107 122 71 631 605 1866 1525 Fragments arranged by size: 1866 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4922 4922 Size: 4927 BlpI GC'TnA_GC Cuts at: 2344 2344 Size: 4927 Bme1580I G_kGCm'C Cuts at: 1790 2988 4144 1790 Size: 1198 1156 2573 Fragments arranged by size: 2573 1198 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4927 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2658 3616 442 Size: 416 53 83 399 24 1241 958 1753 Fragments arranged by size: 1753 1241 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4927 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3587 1239 Size: 2348 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3052 3917 4215 3052 Size: 865 298 3764 Fragments arranged by size: 3764 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3590 882 Size: 617 2091 2219 Fragments arranged by size: 2219 2091 617 BsaAI yAC'GTr Cuts at: 493 1204 2718 493 Size: 711 1514 2702 Fragments arranged by size: 2702 1514 711 BsaBI GATnn'nnATC Cuts at: 4921 4921 Size: 4927 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2734 3116 3751 275 Size: 53 83 186 936 1201 382 635 1451 Fragments arranged by size: 1451 1201 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3360 3758 4032 4179 4590 3360 Size: 398 274 147 411 3697 Fragments arranged by size: 3697 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2181 2211 1178 Size: 30 165 30 778 30 3894 Fragments arranged by size: 3894 778 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4927 BseYI C'CCAG_C Cuts at: 4130 4626 4130 Size: 496 4431 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4354 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2377 3138 4166 1540 Size: 275 186 376 761 1028 2301 Fragments arranged by size: 2301 1028 761 376 275 186 BsiHKAI G_wGCw'C Cuts at: 2988 3073 4144 2988 Size: 85 1071 3771 Fragments arranged by size: 3771 1071 85 BsmI GAATG_Cn' Cuts at: 2438 2531 2438 Size: 93 4834 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2246 2988 3073 4144 1391 Size: 399 456 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 456 399 85 BspEI T'CCGG_A Cuts at: 4590 4590 Size: 4927 BspHI T'CATG_A Cuts at: 2816 4546 2816 Size: 1730 3197 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2282 844 Size: 1438 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2814 1394 Size: 429 991 3507 Fragments arranged by size: 3507 991 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3422 3596 65 Size: 1183 2174 174 1396 Fragments arranged by size: 2174 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3571 3758 1700 Size: 110 1761 187 2869 Fragments arranged by size: 2869 1761 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4927 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4927 BssSI C'ACGA_G Cuts at: 2987 3999 2987 Size: 1012 3915 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4927 BstBI TT'CG_AA Cuts at: 3731 3731 Size: 4927 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4927 BstYI r'GATC_y Cuts at: 1972 2034 2257 2624 3009 3026 4478 4564 4576 Size: 62 223 367 385 17 1452 86 12 Cuts at: 4576 4922 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 223 86 62 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3436 Fragments arranged by size: 3436 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4188 Fragments arranged by size: 4188 683 56 BtsI GCAGTG_nn' Cuts at: 2443 3237 3257 2443 Size: 794 20 4113 Fragments arranged by size: 4113 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4927 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4892 DraI TTT'AAA Cuts at: 2231 2231 Size: 4927 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3934 809 Size: 507 2618 1802 Fragments arranged by size: 2618 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2374 3263 4892 Size: 54 1425 50 275 18 544 889 1629 Cuts at: 4892 8 Size: 43 Fragments arranged by size: 1629 1425 889 544 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2374 1537 Size: 275 562 4090 Fragments arranged by size: 4090 562 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2857 1353 Size: 1504 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3514 3888 4034 387 Size: 1064 138 1925 374 146 1280 Fragments arranged by size: 1925 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2990 3587 4374 4642 1239 Size: 393 1358 597 787 268 1524 Fragments arranged by size: 1524 1358 787 597 393 268 EcoRI G'AATT_C Cuts at: 2236 2614 2236 Size: 378 4549 FspI TGC'GCA Cuts at: 3433 3433 Size: 4927 HaeII r_GCGC'y Cuts at: 1678 4074 1678 Size: 2396 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3568 3600 3642 3674 1373 Size: 32 135 32 1996 32 42 32 2626 Fragments arranged by size: 2626 1996 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2271 2517 669 Size: 988 281 333 246 3079 Fragments arranged by size: 3079 988 333 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4927 HpaI GTT'AAC Cuts at: 2517 2517 Size: 4927 KpnI G_GTAC'C Cuts at: 2252 2252 Size: 4927 MfeI C'AATT_G Cuts at: 2526 2526 Size: 4927 MluI A'CGCG_T Cuts at: 4803 4803 Size: 4927 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4041 4225 1305 Size: 2736 184 2007 Fragments arranged by size: 2736 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3105 Fragments arranged by size: 3105 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2205 2885 3244 3403 518 Size: 648 1039 680 359 159 2042 Fragments arranged by size: 2042 1039 680 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2343 3020 4168 4413 1606 Size: 737 677 1148 245 2120 Fragments arranged by size: 2120 1148 737 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3436 Fragments arranged by size: 3436 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 4927 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4927 NotI GC'GGCC_GC Cuts at: 2374 2374 Size: 4927 NspI r_CATG'y Cuts at: 1132 1229 2285 3830 1132 Size: 97 1056 1545 2229 Fragments arranged by size: 2229 1545 1056 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4927 PciI A'CATG_T Cuts at: 3826 3826 Size: 4927 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4927 PmeI GTTT'AAAC Cuts at: 2231 2231 Size: 4927 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2996 3028 4509 4541 2996 Size: 32 1481 32 3382 Fragments arranged by size: 3382 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4927 PsiI TTA'TAA Cuts at: 2497 2497 Size: 4927 PstI C_TGCA'G Cuts at: 830 2279 830 Size: 1449 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4927 SalI G'TCGA_C Cuts at: 1936 2269 1936 Size: 333 4594 SbfI CC_TGCA'GG Cuts at: 2279 2279 Size: 4927 ScaI AGT'ACT Cuts at: 3175 4887 3175 Size: 1712 3215 SfcI C'TryA_G Cuts at: 826 1045 2275 3410 4091 4282 826 Size: 219 1230 1135 681 191 1471 Fragments arranged by size: 1471 1230 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4927 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4927 SmaI CCC'GGG Cuts at: 1872 2254 1872 Size: 382 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3031 3932 4194 820 Size: 197 926 1088 901 262 1553 Fragments arranged by size: 1553 1088 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2718 493 Size: 2225 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4927 SphI G_CATG'C Cuts at: 1132 2285 1132 Size: 1153 3774 SspI AAT'ATT Cuts at: 5 52 2851 5 Size: 47 2799 2081 Fragments arranged by size: 2799 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4927 StyI C'CwwG_G Cuts at: 513 1065 2004 2366 513 Size: 552 939 362 3074 Fragments arranged by size: 3074 939 552 362 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2954 2971 3124 3309 3754 325 Size: 1255 1374 17 153 185 445 1498 Fragments arranged by size: 1498 1374 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3173 4885 96 Size: 274 80 33 51 1443 1196 1712 138 Fragments arranged by size: 1712 1443 1196 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4887 4919 4887 Size: 32 4895 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4927 XbaI T'CTAG_A Cuts at: 2263 2263 Size: 4927 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4927 XmaI C'CCGG_G Cuts at: 1870 2252 1870 Size: 382 4545 XmnI GAAnn'nnTTC Cuts at: 3056 3056 Size: 4927 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2735 276 Size: 53 83 186 936 1201 2468 Fragments arranged by size: 2468 1201 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI