Restriction map for pFN21AE3802
 (Circular) (Six-base) MAPSORT of: pFN21AE3802.seq  Check: 4644  from: 1  to: 4927



pFN21AE3802  4927 bp

 With 182 enzymes: SgfI * 

                        March 28, 2012 12:12  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2737     278 
   Size:         53      83     186     936    1201    2468

  Fragments arranged by size: 

                2468    1201     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2270    1937 
   Size:        333    4594
 
Acc65I G'GTAC_C

Cuts at:   2248    2248 
   Size:       4927
 
AclI AA'CG_TT

Cuts at:   1563    2226    3054    3427    1563 
   Size:        663     828     373    3063

  Fragments arranged by size: 

                3063     828     663     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2990    4374    4642    1632 
   Size:       1358    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1358     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4730
 
AflIII A'CryG_T

Cuts at:   1203    3826    4803    1203 
   Size:       2623     977    1327

  Fragments arranged by size: 

                2623    1327     977
 
AgeI A'CCGG_T

Cuts at:   3758    3758 
   Size:       4927
 
AhdI GACnn_n'nnGTC

Cuts at:   3656    3656 
   Size:       4927
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       4927
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       4927
 
AlwNI CAG_nnn'CTG

Cuts at:   2150    2670    4242    2150 
   Size:        520    1572    2835

  Fragments arranged by size: 

                2835    1572     520
 
ApaLI G'TGCA_C

Cuts at:   2984    4140    2984 
   Size:       1156    3771
 
ApoI r'AATT_y

Cuts at:   1493    2236    2462    2614    4723    1493 
   Size:        743     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     743     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3481     160 
   Size:        872    2449    1606

  Fragments arranged by size: 

                2449    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       4927
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2252    1870 
   Size:         73     309    4545

  Fragments arranged by size: 

                4545     309      73
 
BamHI G'GATC_C

Cuts at:   2257    2624    2257 
   Size:        367    4560
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2138    2248    3703     618 
   Size:        325     217     627     351     110    1455    1842

  Fragments arranged by size: 

                1842    1455     627     351     325     217     110
 
BanII G_rGCy'C

Cuts at:   1391    2246    1391 
   Size:        855    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2073     928 
   Size:        971     174    3782

  Fragments arranged by size: 

                3782     971     174
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3118    3152    1903 
   Size:         34    1181      34    3678

  Fragments arranged by size: 

                3678    1181      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2819    4029    2819 
   Size:       1210    3717
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       4927
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3538     136 
   Size:        107     122      71     631     605    1866    1525

  Fragments arranged by size: 

                1866    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   4922    4922 
   Size:       4927
 
BlpI GC'TnA_GC

Cuts at:   2344    2344 
   Size:       4927
 
Bme1580I G_kGCm'C

Cuts at:   1790    2988    4144    1790 
   Size:       1198    1156    2573

  Fragments arranged by size: 

                2573    1198    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       4927
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2658    3616     442 
   Size:        416      53      83     399      24    1241     958    1753

  Fragments arranged by size: 

                1753    1241     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       4927
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3587    1239 
   Size:       2348    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3052    3917    4215    3052 
   Size:        865     298    3764

  Fragments arranged by size: 

                3764     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3590     882 
   Size:        617    2091    2219

  Fragments arranged by size: 

                2219    2091     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2718     493 
   Size:        711    1514    2702

  Fragments arranged by size: 

                2702    1514     711
 
BsaBI GATnn'nnATC

Cuts at:   4921    4921 
   Size:       4927
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2734    3116    3751     275 
   Size:         53      83     186     936    1201     382     635    1451

  Fragments arranged by size: 

                1451    1201     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3360    3758    4032    4179    4590    3360 
   Size:        398     274     147     411    3697

  Fragments arranged by size: 

                3697     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2181    2211    1178 
   Size:         30     165      30     778      30    3894

  Fragments arranged by size: 

                3894     778     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       4927
 
BseYI C'CCAG_C

Cuts at:   4130    4626    4130 
   Size:        496    4431
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4354
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2377    3138    4166    1540 
   Size:        275     186     376     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     376     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2988    3073    4144    2988 
   Size:         85    1071    3771

  Fragments arranged by size: 

                3771    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2438    2531    2438 
   Size:         93    4834
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2246    2988    3073    4144    1391 
   Size:        399     456     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     456     399      85
 
BspEI T'CCGG_A

Cuts at:   4590    4590 
   Size:       4927
 
BspHI T'CATG_A

Cuts at:   2816    4546    2816 
   Size:       1730    3197
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2282     844 
   Size:       1438    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2814    1394 
   Size:        429     991    3507

  Fragments arranged by size: 

                3507     991     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3422    3596      65 
   Size:       1183    2174     174    1396

  Fragments arranged by size: 

                2174    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3571    3758    1700 
   Size:        110    1761     187    2869

  Fragments arranged by size: 

                2869    1761     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       4927
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       4927
 
BssSI C'ACGA_G

Cuts at:   2987    3999    2987 
   Size:       1012    3915
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       4927
 
BstBI TT'CG_AA

Cuts at:   3731    3731 
   Size:       4927
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       4927
 
BstYI r'GATC_y

Cuts at:   1972    2034    2257    2624    3009    3026    4478    4564    4576 
   Size:         62     223     367     385      17    1452      86      12

Cuts at:   4576    4922    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     385     367     346     223      86      62
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3436

  Fragments arranged by size: 

                3436     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4188

  Fragments arranged by size: 

                4188     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2443    3237    3257    2443 
   Size:        794      20    4113

  Fragments arranged by size: 

                4113     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       4927
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    4892
 
DraI TTT'AAA

Cuts at:   2231    2231 
   Size:       4927
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    3934     809 
   Size:        507    2618    1802

  Fragments arranged by size: 

                2618    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2374    3263    4892 
   Size:         54    1425      50     275      18     544     889    1629

Cuts at:   4892       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     544     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2374    1537 
   Size:        275     562    4090

  Fragments arranged by size: 

                4090     562     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2857    1353 
   Size:       1504    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3514    3888    4034     387 
   Size:       1064     138    1925     374     146    1280

  Fragments arranged by size: 

                1925    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2990    3587    4374    4642    1239 
   Size:        393    1358     597     787     268    1524

  Fragments arranged by size: 

                1524    1358     787     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2236    2614    2236 
   Size:        378    4549
 
FspI TGC'GCA

Cuts at:   3433    3433 
   Size:       4927
 
HaeII r_GCGC'y

Cuts at:   1678    4074    1678 
   Size:       2396    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3568    3600    3642    3674    1373 
   Size:         32     135      32    1996      32      42      32    2626

  Fragments arranged by size: 

                2626    1996     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2271    2517     669 
   Size:        988     281     333     246    3079

  Fragments arranged by size: 

                3079     988     333     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       4927
 
HpaI GTT'AAC

Cuts at:   2517    2517 
   Size:       4927
 
KpnI G_GTAC'C

Cuts at:   2252    2252 
   Size:       4927
 
MfeI C'AATT_G

Cuts at:   2526    2526 
   Size:       4927
 
MluI A'CGCG_T

Cuts at:   4803    4803 
   Size:       4927
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4041    4225    1305 
   Size:       2736     184    2007

  Fragments arranged by size: 

                2736    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3105

  Fragments arranged by size: 

                3105    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2205    2885    3244    3403     518 
   Size:        648    1039     680     359     159    2042

  Fragments arranged by size: 

                2042    1039     680     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2343    3020    4168    4413    1606 
   Size:        737     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     737     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3436

  Fragments arranged by size: 

                3436     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       4927
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       4927
 
NotI GC'GGCC_GC

Cuts at:   2374    2374 
   Size:       4927
 
NspI r_CATG'y

Cuts at:   1132    1229    2285    3830    1132 
   Size:         97    1056    1545    2229

  Fragments arranged by size: 

                2229    1545    1056      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       4927
 
PciI A'CATG_T

Cuts at:   3826    3826 
   Size:       4927
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       4927
 
PmeI GTTT'AAAC

Cuts at:   2231    2231 
   Size:       4927
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2996    3028    4509    4541    2996 
   Size:         32    1481      32    3382

  Fragments arranged by size: 

                3382    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       4927
 
PsiI TTA'TAA

Cuts at:   2497    2497 
   Size:       4927
 
PstI C_TGCA'G

Cuts at:    830    2279     830 
   Size:       1449    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       4927
 
SalI G'TCGA_C

Cuts at:   1936    2269    1936 
   Size:        333    4594
 
SbfI CC_TGCA'GG

Cuts at:   2279    2279 
   Size:       4927
 
ScaI AGT'ACT

Cuts at:   3175    4887    3175 
   Size:       1712    3215
 
SfcI C'TryA_G

Cuts at:    826    1045    2275    3410    4091    4282     826 
   Size:        219    1230    1135     681     191    1471

  Fragments arranged by size: 

                1471    1230    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       4927
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       4927
 
SmaI CCC'GGG

Cuts at:   1872    2254    1872 
   Size:        382    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3031    3932    4194     820 
   Size:        197     926    1088     901     262    1553

  Fragments arranged by size: 

                1553    1088     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2718     493 
   Size:       2225    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       4927
 
SphI G_CATG'C

Cuts at:   1132    2285    1132 
   Size:       1153    3774
 
SspI AAT'ATT

Cuts at:      5      52    2851       5 
   Size:         47    2799    2081

  Fragments arranged by size: 

                2799    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       4927
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2366     513 
   Size:        552     939     362    3074

  Fragments arranged by size: 

                3074     939     552     362
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2954    2971    3124    3309    3754     325 
   Size:       1255    1374      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1374    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3173    4885      96 
   Size:        274      80      33      51    1443    1196    1712     138

  Fragments arranged by size: 

                1712    1443    1196     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4887    4919    4887 
   Size:         32    4895
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       4927
 
XbaI T'CTAG_A

Cuts at:   2263    2263 
   Size:       4927
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       4927
 
XmaI C'CCGG_G

Cuts at:   1870    2252    1870 
   Size:        382    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3056    3056 
   Size:       4927
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2735     276 
   Size:         53      83     186     936    1201    2468

  Fragments arranged by size: 

                2468    1201     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI    EcoRI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI
     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI
     PsiI     PstI     PvuI     SalI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI
     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII
     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI
     SwaI     XcmI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com