################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI ItaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TseI TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AlwI 601 GATCC GGATC(4/5) AseI 79 ATTAAT AT^TAAT AsnI 79 ATTAAT AT^TAAT Asp700I 93 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 122 GGTCC G^G[AT]CC BlpI 159 GCTGAGC GC^T[ACGT]AGC Bpu1102I 159 GCTGAGC GC^T[ACGT]AGC BsaBI 214 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 125 CCCAGG C^C[ACGT][ACGT]GG BseRI 40 CTCCTC GAGGAG(10/8) BsiYI 126 CCAGGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 126 CCAGGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 123 GTCCC GGGAC(10/14) BsrI 144 CCAGT ACTGG(1/-1) BssKI 126 CCAGG ^CC[ACGT]GG BstNI 126 CCAGG CC^[AT]GG BstYI 600 AGATCC [GA]^GATC[CT] CelII 159 GCTGAGC GC^T[ACGT]AGC Cfr13I 122 GGTCC G^G[ACGT]CC DraII 121 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoO109I 121 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoRII 126 CCAGG ^CC[AT]GG HgaI 36 GCGTC GACGC(5/10) MaeII 153 ACGT A^CGT MaeIII 10 GTGAC ^GT[ACGT]AC MamI 214 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 600 AGATCC [GA]^GATC[CT] MvaI 126 CCAGG CC^[AT]GG PpuMI 121 GGGTCCC [GA]G^G[AT]CC[CT] PshBI 79 ATTAAT AT^TAAT Sau96I 122 GGTCC G^G[ACGT]CC ScrFI 126 CCAGG CC^[ACGT]GG SpeI 204 ACTAGT A^CTAGT Tsp45I 10 GTGAC ^GT[GC]AC XhoII 600 AGATCC [GA]^GATC[CT] XmnI 93 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 147 GTAC GT^AC 515 GTAC AspHI 162 GAGCAC G[AT]GC[AT]^C 281 GAGCAC BmyI 162 GAGCAC G[AGT]GC[ACT]^C 281 GAGCAC BpmI 62 CTGGAG CTGGAG(16/14) 278 CTGGAG BsiHKAI 162 GAGCAC G[AT]GC[AT]^C 281 GAGCAC BsmBI 37 CGTCTC CGTCTC(1/5) 486 GAGACG Bsp1286I 162 GAGCAC G[AGT]GC[ACT]^C 281 GAGCAC BstF5I 385 CATCC GGATG(2/0) 519 CATCC Cac8I 164 GCACGC GC[ACGT]^[ACGT]GC 586 GCAAGC DpnII 70 GATC ^GATC 601 GATC EarI 93 GAAGAG CTCTTC(1/4) 130 GAAGAG FokI 385 CATCC GGATG(9/13) 519 CATCC HinfI 219 GAATC G^A[ACGT]TC 375 GATTC Ksp632I 93 GAAGAG CTCTTC(1/4) 130 GAAGAG MboI 70 GATC ^GATC 601 GATC MwoI 159 GCTGAGCACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 577 GCTCAGGAAGC NdeII 70 GATC ^GATC 601 GATC RsaI 147 GTAC GT^AC 515 GTAC Sau3AI 70 GATC ^GATC 601 GATC SfaNI 358 GATGC GCATC(5/9) 441 GCATC TfiI 219 GAATC G^A[AT]TC 375 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AcsI AluI ApoI BfaI BsmAI DdeI MaeI MboII MnlI MseI NlaIII NlaIV Tru9I Tsp509I