(Circular) (Six-base) MAPSORT of: pFN21AE1227.seq Check: 8904 from: 1 to: 5644 pFN21AE1227 5644 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2486 2486 Size: 5644 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3454 278 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2987 1937 Size: 1050 4594 Acc65I G'GTAC_C Cuts at: 2965 2965 Size: 5644 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3707 5091 5359 1632 Size: 2075 1384 268 1917 Fragments arranged by size: 2075 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5447 AflIII A'CryG_T Cuts at: 1203 4543 5520 1203 Size: 3340 977 1327 Fragments arranged by size: 3340 1327 977 AgeI A'CCGG_T Cuts at: 4475 4475 Size: 5644 AhdI GACnn_n'nnGTC Cuts at: 4373 4373 Size: 5644 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5644 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5644 AlwNI CAG_nnn'CTG Cuts at: 3387 4959 3387 Size: 1572 4072 ApaLI G'TGCA_C Cuts at: 3701 4857 3701 Size: 1156 4488 ApoI r'AATT_y Cuts at: 1493 2606 2953 3179 3331 5440 1493 Size: 1113 347 226 152 2109 1697 Fragments arranged by size: 2109 1697 1113 347 226 152 AseI AT'TA_AT Cuts at: 160 1032 4198 160 Size: 872 3166 1606 Fragments arranged by size: 3166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2116 2969 1870 Size: 73 173 853 4545 Fragments arranged by size: 4545 853 173 73 BamHI G'GATC_C Cuts at: 2974 3341 2974 Size: 367 5277 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2965 4420 618 Size: 325 217 627 1178 1455 1842 Fragments arranged by size: 1842 1455 1178 627 325 217 BanII G_rGCy'C Cuts at: 1391 2963 1391 Size: 1572 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4673 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2561 2595 3835 3869 1903 Size: 34 624 34 1240 34 3678 Fragments arranged by size: 3678 1240 624 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3536 4746 3536 Size: 1210 4434 BclI T'GATC_A Cuts at: 1548 1548 Size: 5644 BfrBI ATG'CAT Cuts at: 2440 2672 2440 Size: 232 5412 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2100 2160 4255 Size: 107 122 71 631 605 428 60 2095 Cuts at: 4255 136 Size: 1525 Fragments arranged by size: 2095 1525 631 605 428 122 107 71 60 BglII A'GATC_T Cuts at: 5639 5639 Size: 5644 BlpI GC'TnA_GC Cuts at: 3061 3061 Size: 5644 Bme1580I G_kGCm'C Cuts at: 1790 3705 4861 1790 Size: 1915 1156 2573 Fragments arranged by size: 2573 1915 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5644 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3375 4333 442 Size: 416 53 83 399 24 1958 958 1753 Fragments arranged by size: 1958 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5644 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2733 2753 4304 1239 Size: 1494 20 1551 2579 Fragments arranged by size: 2579 1551 1494 20 Bpu10I CC'TnA_GC Cuts at: 2046 2046 Size: 5644 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3769 4634 4932 3769 Size: 865 298 4481 Fragments arranged by size: 4481 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2385 2421 4307 882 Size: 617 886 36 1886 2219 Fragments arranged by size: 2219 1886 886 617 36 BsaAI yAC'GTr Cuts at: 493 1204 3435 493 Size: 711 2231 2702 Fragments arranged by size: 2702 2231 711 BsaBI GATnn'nnATC Cuts at: 5638 5638 Size: 5644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3451 3833 4468 275 Size: 53 83 186 936 1918 382 635 1451 Fragments arranged by size: 1918 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2412 4077 4475 4749 4896 5307 2412 Size: 1665 398 274 147 411 2749 Fragments arranged by size: 2749 1665 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2117 2147 1178 Size: 30 165 30 714 30 4675 Fragments arranged by size: 4675 714 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5644 BseYI C'CCAG_C Cuts at: 4847 5343 4847 Size: 496 5148 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5071 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3094 3855 4883 1540 Size: 275 186 1093 761 1028 2301 Fragments arranged by size: 2301 1093 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2145 3705 3790 4861 2145 Size: 1560 85 1071 2928 Fragments arranged by size: 2928 1560 1071 85 BsmI GAATG_Cn' Cuts at: 2672 3155 3248 2672 Size: 483 93 5068 Fragments arranged by size: 5068 483 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2099 2099 Size: 5644 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2963 3705 3790 4861 1391 Size: 399 355 818 742 85 1071 2174 Fragments arranged by size: 2174 1071 818 742 399 355 85 BspEI T'CCGG_A Cuts at: 5307 5307 Size: 5644 BspHI T'CATG_A Cuts at: 2886 3533 5263 2886 Size: 647 1730 3267 Fragments arranged by size: 3267 1730 647 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2486 2999 844 Size: 1642 513 3489 Fragments arranged by size: 3489 1642 513 BsrBI CCG'CTC Cuts at: 1394 1823 2814 3531 1394 Size: 429 991 717 3507 Fragments arranged by size: 3507 991 717 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2620 4139 4313 65 Size: 1183 1372 1519 174 1396 Fragments arranged by size: 1519 1396 1372 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4288 4475 1700 Size: 110 2478 187 2869 Fragments arranged by size: 2869 2478 187 110 BsrGI T'GTAC_A Cuts at: 96 2181 96 Size: 2085 3559 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5644 BssSI C'ACGA_G Cuts at: 3704 4716 3704 Size: 1012 4632 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5644 BstBI TT'CG_AA Cuts at: 4448 4448 Size: 5644 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5644 BstYI r'GATC_y Cuts at: 1972 2407 2790 2974 3341 3726 3743 5195 5281 Size: 435 383 184 367 385 17 1452 86 Cuts at: 5281 5293 5639 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 435 385 383 367 346 184 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5092 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4905 Fragments arranged by size: 4905 683 56 BtsI GCAGTG_nn' Cuts at: 3160 3954 3974 3160 Size: 794 20 4830 Fragments arranged by size: 4830 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5609 DraI TTT'AAA Cuts at: 2948 2948 Size: 5644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4651 809 Size: 507 3335 1802 Fragments arranged by size: 3335 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3091 3980 5609 Size: 54 1425 50 275 18 1261 889 1629 Cuts at: 5609 8 Size: 43 Fragments arranged by size: 1629 1425 1261 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3091 1537 Size: 275 1279 4090 Fragments arranged by size: 4090 1279 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3574 1353 Size: 2221 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4231 4605 4751 387 Size: 1064 138 2642 374 146 1280 Fragments arranged by size: 2642 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2733 2753 3707 4304 5091 5359 1239 Size: 393 1101 20 954 597 787 268 1524 Fragments arranged by size: 1524 1101 954 787 597 393 268 20 EcoO109I rG'GnC_Cy Cuts at: 2431 2431 Size: 5644 EcoRI G'AATT_C Cuts at: 2953 3331 2953 Size: 378 5266 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 4791 1678 Size: 3113 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2117 2149 2399 2431 4285 Size: 32 135 32 545 32 250 32 1854 Cuts at: 4285 4317 4359 4391 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1854 545 250 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2503 2988 3234 669 Size: 988 281 565 485 246 3079 Fragments arranged by size: 3079 988 565 485 281 246 HindIII A'AGCT_T Cuts at: 748 2519 748 Size: 1771 3873 HpaI GTT'AAC Cuts at: 3234 3234 Size: 5644 KpnI G_GTAC'C Cuts at: 2969 2969 Size: 5644 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2814 2934 4758 4942 1305 Size: 1509 120 1824 184 2007 Fragments arranged by size: 2007 1824 1509 184 120 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3822 Fragments arranged by size: 3822 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3602 3961 4120 518 Size: 648 2436 359 159 2042 Fragments arranged by size: 2436 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3060 3737 4885 5130 1606 Size: 1454 677 1148 245 2120 Fragments arranged by size: 2120 1454 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5092 NdeI CA'TA_TG Cuts at: 387 387 Size: 5644 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5644 NotI GC'GGCC_GC Cuts at: 3091 3091 Size: 5644 NsiI A_TGCA'T Cuts at: 2442 2674 2442 Size: 232 5412 NspI r_CATG'y Cuts at: 1132 1229 3002 4547 1132 Size: 97 1773 1545 2229 Fragments arranged by size: 2229 1773 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5644 PciI A'CATG_T Cuts at: 4543 4543 Size: 5644 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5644 PfoI T'CCnGG_A Cuts at: 2314 2314 Size: 5644 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3713 3745 5226 5258 3713 Size: 32 1481 32 4099 Fragments arranged by size: 4099 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5644 PsiI TTA'TAA Cuts at: 3214 3214 Size: 5644 PstI C_TGCA'G Cuts at: 830 2448 2497 2996 830 Size: 1618 49 499 3478 Fragments arranged by size: 3478 1618 499 49 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5644 SalI G'TCGA_C Cuts at: 1936 2986 1936 Size: 1050 4594 SbfI CC_TGCA'GG Cuts at: 2996 2996 Size: 5644 ScaI AGT'ACT Cuts at: 3892 5604 3892 Size: 1712 3932 SfcI C'TryA_G Cuts at: 826 1045 2444 2493 2992 4127 4808 4999 826 Size: 219 1399 49 499 1135 681 191 1471 Fragments arranged by size: 1471 1399 1135 681 499 219 191 49 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2971 1872 Size: 1099 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2116 3748 4649 4911 820 Size: 197 926 173 1632 901 262 1553 Fragments arranged by size: 1632 1553 926 901 262 197 173 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 3002 1132 Size: 1870 3774 SspI AAT'ATT Cuts at: 5 52 3568 5 Size: 47 3516 2081 Fragments arranged by size: 3516 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5644 StyI C'CwwG_G Cuts at: 513 1065 3083 513 Size: 552 2018 3074 Fragments arranged by size: 3074 2018 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2030 3671 3688 3841 4026 4471 325 Size: 1255 450 1641 17 153 185 445 1498 Fragments arranged by size: 1641 1498 1255 450 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 3890 5602 Size: 274 80 33 51 1443 204 1709 1712 Cuts at: 5602 96 Size: 138 Fragments arranged by size: 1712 1709 1443 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5604 5636 5604 Size: 32 5612 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5644 XbaI T'CTAG_A Cuts at: 2980 2980 Size: 5644 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2353 2801 2353 Size: 448 5196 XhoI C'TCGA_G Cuts at: 1943 2116 1943 Size: 173 5471 XmaI C'CCGG_G Cuts at: 1870 2969 1870 Size: 1099 4545 XmnI GAAnn'nnTTC Cuts at: 3773 3773 Size: 5644 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3452 276 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI