Restriction map for
pFN21AE9522
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BamHI BbrPI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspHI BspLU11I BsrBI BssHII
Bst1107I BstEII BstPI BstZ17I CelII
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI HindIII KpnI
KspI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AocI 1799 CCTCAGG CC^T[ACGT]AGG
AspI 2601 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1084 TGCGCA TGC^GCA
BbeI 1244 GGCGCC GGCGC^C
BbsI 1837 GTCTTC GAAGAC(2/6)
BcgI 2053 GCACTTATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 676 CTAG C^TAG
BpuAI 1837 GTCTTC GAAGAC(2/6)
BseRI 2209 CTCCTC GAGGAG(10/8)
Bsp1407I 2297 TGTACA T^GTACA
BsrFI 1474 GCCGGC [GA]^CCGG[CT]
BsrGI 2297 TGTACA T^GTACA
BssSI 683 CTCGTG CACGAG(-5/-1)
BstBI 1660 TTCGAA TT^CGAA
BstYI 476 AGATCC [GA]^GATC[CT]
Bsu36I 1799 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1474 GCCGGC [GA]^CCGG[CT]
DraIII 2376 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 612 GACCTATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1799 CCTCAGG CC^T[ACGT]AGG
EcoNI 1251 CCTCTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1084 TGCGCA TGC^GCA
HincII 1237 GTTAAC GT[CT]^[GA]AC
HindII 1237 GTTAAC GT[CT]^[GA]AC
HpaI 1237 GTTAAC GTT^AAC
KasI 1244 GGCGCC G^GCGCC
MaeI 676 CTAG C^TAG
MflI 476 AGATCC [GA]^GATC[CT]
MseI 1238 TTAA T^TAA
NaeI 1474 GCCGGC GCC^GGC
NarI 1244 GGCGCC GG^CGCC
NgoMIV 1474 GCCGGC G^CCGGC
NspV 1660 TTCGAA TT^CGAA
PflFI 2601 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1707 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 145 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1810 GAGCTC GAGCT^C
SfoI 1244 GGCGCC GGC^GCC
SfuI 1660 TTCGAA TT^CGAA
SmlI 462 CTTGAG C^T[CT][GA]AG
SspBI 2297 TGTACA T^GTACA
TfiI 591 GATTC G^A[AT]TC
Tru9I 1238 TTAA T^TAA
Tth111I 2601 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 782 CCAGTGCCCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 476 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 1039 GAATTC [GA]^AATT[CT]
1600 GAATTC
Alw44I 118 GTGCAC G^TGCAC
254 GTGCAC
AlwI 477 GATCC GGATC(4/5)
4 GGATC
ApaLI 118 GTGCAC G^TGCAC
254 GTGCAC
ApoI 1039 GAATTC [GA]^AATT[CT]
1600 GAATTC
BsmI 1565 GCATTC GAATGC(1/-1)
2323 GCATTC
BspMI 230 ACCTGC ACCTGC(4/8)
2305 ACCTGC
BstAPI 228 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1565 GCATTCCGTGC
EarI 2452 CTCTTC CTCTTC(1/4)
1527 GAAGAG
EcoRI 1039 GAATTC G^AATTC
1600 GAATTC
Ksp632I 2452 CTCTTC CTCTTC(1/4)
1527 GAAGAG
NspI 288 ACATGC [GA]CATG^[CT]
1181 ACATGC
PvuII 1301 CAGCTG CAG^CTG
2068 CAGCTG
SmaI 824 CCCGGG CCC^GGG
1991 CCCGGG
StuI 665 AGGCCT AGG^CCT
1796 AGGCCT
Tsp509I 1040 AATT ^AATT
1601 AATT
XmaCI 824 CCCGGG C^CCGGG
1991 CCCGGG
XmaI 824 CCCGGG C^CCGGG
1991 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I