(Circular) (Six-base) MAPSORT of: pFN21AE9522.seq Check: 1881 from: 1 to: 7321 pFN21AE9522 7321 bp With 182 enzymes: SgfI * July 4, 2020 18:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2244 4319 2244 Size: 2075 5246 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5131 278 Size: 53 83 186 936 3595 2468 Fragments arranged by size: 3595 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4664 1937 Size: 2727 4594 Acc65I G'GTAC_C Cuts at: 4642 4642 Size: 7321 AclI AA'CG_TT Cuts at: 1563 5448 5821 1563 Size: 3885 373 3063 Fragments arranged by size: 3885 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5384 6768 7036 1632 Size: 3752 1384 268 1917 Fragments arranged by size: 3752 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7124 AflIII A'CryG_T Cuts at: 1203 6220 7197 1203 Size: 5017 977 1327 Fragments arranged by size: 5017 1327 977 AgeI A'CCGG_T Cuts at: 6152 6152 Size: 7321 AhdI GACnn_n'nnGTC Cuts at: 6050 6050 Size: 7321 AleI CACnn'nnGTG Cuts at: 1166 2876 3791 3893 1166 Size: 1710 915 102 4594 Fragments arranged by size: 4594 1710 915 102 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7321 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3044 3076 3044 Size: 32 7289 AlwNI CAG_nnn'CTG Cuts at: 2188 2311 3004 5064 6636 2188 Size: 123 693 2060 1572 2873 Fragments arranged by size: 2873 2060 1572 693 123 ApaI G_GGCC'C Cuts at: 2066 2902 3997 2066 Size: 836 1095 5390 Fragments arranged by size: 5390 1095 836 ApaLI G'TGCA_C Cuts at: 2123 2259 5378 6534 2123 Size: 136 3119 1156 2910 Fragments arranged by size: 3119 2910 1156 136 ApoI r'AATT_y Cuts at: 1493 3044 3605 4630 4856 5008 7117 1493 Size: 1551 561 1025 226 152 2109 1697 Fragments arranged by size: 2109 1697 1551 1025 561 226 152 AseI AT'TA_AT Cuts at: 160 1032 5875 160 Size: 872 4843 1606 Fragments arranged by size: 4843 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7321 AvaI C'yCGr_G Cuts at: 1870 1943 2829 3996 4066 4646 1870 Size: 73 886 1167 70 580 4545 Fragments arranged by size: 4545 1167 886 580 73 70 BamHI G'GATC_C Cuts at: 4651 5018 4651 Size: 367 6954 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3249 3737 4004 4108 4492 Size: 325 217 627 1462 488 267 104 384 Cuts at: 4492 4642 6097 618 Size: 150 1455 1842 Fragments arranged by size: 1842 1462 1455 627 488 384 325 267 217 150 104 BanII G_rGCy'C Cuts at: 1391 2066 2773 2902 3513 3819 3997 4196 4640 Size: 675 707 129 611 306 178 199 444 Cuts at: 4640 1391 Size: 4072 Fragments arranged by size: 4072 707 675 611 444 306 199 178 129 BbeI G_GCGC'C Cuts at: 3253 3253 Size: 7321 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3835 928 Size: 971 1936 4414 Fragments arranged by size: 4414 1936 971 BbvCI CC'TCA_GC Cuts at: 2058 2937 3475 4403 2058 Size: 879 538 928 4976 Fragments arranged by size: 4976 928 879 538 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4047 4081 5512 5546 1903 Size: 34 2110 34 1431 34 3678 Fragments arranged by size: 3678 2110 1431 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5213 6423 5213 Size: 1210 6111 BclI T'GATC_A Cuts at: 1548 1548 Size: 7321 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2373 2798 3657 Size: 107 122 71 631 605 701 425 859 Cuts at: 3657 4407 4509 5932 136 Size: 750 102 1423 1525 Fragments arranged by size: 1525 1423 859 750 701 631 605 425 122 107 102 71 BglII A'GATC_T Cuts at: 7316 7316 Size: 7321 BlpI GC'TnA_GC Cuts at: 4738 4738 Size: 7321 Bme1580I G_kGCm'C Cuts at: 1790 2066 2127 2263 2695 2794 2902 3117 3199 Size: 276 61 136 432 99 108 215 82 Cuts at: 3199 3742 3926 3997 4009 4118 4262 4495 5382 Size: 543 184 71 12 109 144 233 887 Cuts at: 5382 6538 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 887 543 432 276 233 215 184 144 136 109 108 99 82 71 61 12 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7321 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2114 2781 3433 Size: 416 53 83 399 24 697 667 652 Cuts at: 3433 4555 5052 6010 442 Size: 1122 497 958 1753 Fragments arranged by size: 1753 1122 958 697 667 652 497 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7321 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2980 2980 Size: 7321 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 2210 2321 2924 3503 4339 4519 5981 Size: 869 102 111 603 579 836 180 1462 Cuts at: 5981 1239 Size: 2579 Fragments arranged by size: 2579 1462 869 836 603 579 180 111 102 Bpu10I CC'TnA_GC Cuts at: 2058 2937 3475 4403 2058 Size: 879 538 928 4976 Fragments arranged by size: 4976 928 879 538 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2488 5446 6311 6609 2488 Size: 2958 865 298 3200 Fragments arranged by size: 3200 2958 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5984 882 Size: 617 4485 2219 Fragments arranged by size: 4485 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5112 493 Size: 711 3908 2702 Fragments arranged by size: 3908 2702 711 BsaBI GATnn'nnATC Cuts at: 7315 7315 Size: 7321 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2636 3250 3910 5128 Size: 53 83 186 936 1103 614 660 1218 Cuts at: 5128 5510 6145 275 Size: 382 635 1451 Fragments arranged by size: 1451 1218 1103 936 660 635 614 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5754 6152 6426 6573 6984 5754 Size: 398 274 147 411 6091 Fragments arranged by size: 6091 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2085 2115 2264 2294 2545 Size: 30 165 30 682 30 149 30 251 Cuts at: 2545 2575 2972 3002 3044 3074 1178 Size: 30 397 30 42 30 5425 Fragments arranged by size: 5425 682 397 251 165 149 42 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4205 1186 Size: 3019 4302 BseYI C'CCAG_C Cuts at: 2065 2104 2140 3304 3690 3760 3832 4008 6524 Size: 39 36 1164 386 70 72 176 2516 Cuts at: 6524 7020 2065 Size: 496 2366 Fragments arranged by size: 2516 2366 1164 496 386 176 72 70 39 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3405 3560 4213 4430 1163 Size: 573 1669 155 653 217 4054 Fragments arranged by size: 4054 1669 653 573 217 155 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4771 5532 6560 1540 Size: 275 186 2770 761 1028 2301 Fragments arranged by size: 2770 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2127 2172 2263 3276 3819 4215 5382 5467 6538 Size: 45 91 1013 543 396 1167 85 1071 Cuts at: 6538 2127 Size: 2910 Fragments arranged by size: 2910 1167 1071 1013 543 396 91 85 45 BsmI GAATG_Cn' Cuts at: 3570 4328 4832 4925 3570 Size: 758 504 93 5966 Fragments arranged by size: 5966 758 504 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2127 2172 2263 2695 2773 2794 Size: 399 276 61 45 91 432 78 21 Cuts at: 2794 2902 3117 3199 3276 3513 3742 3819 3926 Size: 108 215 82 77 237 229 77 107 Cuts at: 3926 3997 4009 4118 4196 4215 4262 4495 4640 Size: 71 12 109 78 19 47 233 145 Cuts at: 4640 5382 5467 6538 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 432 399 276 237 233 229 215 145 109 108 107 91 85 82 78 78 77 77 71 61 47 45 21 19 12 BspEI T'CCGG_A Cuts at: 6984 6984 Size: 7321 BspHI T'CATG_A Cuts at: 5210 6940 5210 Size: 1730 5591 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2244 4319 4676 844 Size: 1400 2075 357 3489 Fragments arranged by size: 3489 2075 1400 357 BsrBI CCG'CTC Cuts at: 1394 1823 5208 1394 Size: 429 3385 3507 Fragments arranged by size: 3507 3385 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3873 3972 4588 5816 5990 65 Size: 1183 2625 99 616 1228 174 1396 Fragments arranged by size: 2625 1396 1228 1183 616 174 99 BsrFI r'CCGG_y Cuts at: 1700 1810 3479 5965 6152 1700 Size: 110 1669 2486 187 2869 Fragments arranged by size: 2869 2486 1669 187 110 BsrGI T'GTAC_A Cuts at: 96 4302 96 Size: 4206 3115 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7321 BssSI C'ACGA_G Cuts at: 2688 5381 6393 2688 Size: 2693 1012 3616 Fragments arranged by size: 3616 2693 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2239 3576 794 Size: 1445 1337 4539 Fragments arranged by size: 4539 1445 1337 BstBI TT'CG_AA Cuts at: 3666 6125 3666 Size: 2459 4862 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7321 BstXI CCAn_nnnn'nTGG Cuts at: 2455 2587 2959 3793 4054 4553 2455 Size: 132 372 834 261 499 5223 Fragments arranged by size: 5223 834 499 372 261 132 BstYI r'GATC_y Cuts at: 1972 2481 4651 5018 5403 5420 6872 6958 6970 Size: 509 2170 367 385 17 1452 86 12 Cuts at: 6970 7316 1972 Size: 346 1977 Fragments arranged by size: 2170 1977 1452 509 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3805 3805 Size: 7321 BtgI C'CryG_G Cuts at: 513 1065 2004 2173 2338 2389 2586 3165 3461 Size: 552 939 169 165 51 197 579 296 Cuts at: 3461 3888 4413 513 Size: 427 525 3421 Fragments arranged by size: 3421 939 579 552 525 427 296 197 169 165 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3245 3709 3711 4191 4204 4599 Size: 683 56 2019 464 2 480 13 395 Cuts at: 4599 487 Size: 3209 Fragments arranged by size: 3209 2019 683 480 464 395 56 13 2 BtsI GCAGTG_nn' Cuts at: 2030 2403 2929 3162 3397 3511 3900 3924 4157 Size: 373 526 233 235 114 389 24 233 Cuts at: 4157 4519 4837 5631 5651 2030 Size: 362 318 794 20 3700 Fragments arranged by size: 3700 794 526 389 373 362 318 235 233 233 114 24 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7321 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3736 3771 529 Size: 35 3172 35 4079 Fragments arranged by size: 4079 3172 35 35 DraI TTT'AAA Cuts at: 4625 4625 Size: 7321 DraIII CAC_nnn'GTG Cuts at: 4386 4386 Size: 7321 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2623 6328 809 Size: 507 1307 3705 1802 Fragments arranged by size: 3705 1802 1307 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2457 2709 2880 Size: 54 1425 50 275 18 627 252 171 Cuts at: 2880 3236 3355 3744 4013 4044 4543 4585 4768 Size: 356 119 389 269 31 499 42 183 Cuts at: 4768 5657 7286 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 627 499 389 356 275 269 252 183 171 119 54 50 43 42 31 18 EagI C'GGCC_G Cuts at: 1537 1812 4768 1537 Size: 275 2956 4090 Fragments arranged by size: 4090 2956 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3527 4463 5251 1353 Size: 2174 936 788 3423 Fragments arranged by size: 3423 2174 936 788 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5908 6282 6428 387 Size: 1064 138 4319 374 146 1280 Fragments arranged by size: 4319 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3817 3817 Size: 7321 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2210 2321 2924 3503 4339 4519 Size: 393 476 102 111 603 579 836 180 Cuts at: 4519 5384 5981 6768 7036 1239 Size: 865 597 787 268 1524 Fragments arranged by size: 1524 865 836 787 603 597 579 476 393 268 180 111 102 EcoNI CCTnn'n_nnAGG Cuts at: 3260 3260 Size: 7321 EcoO109I rG'GnC_Cy Cuts at: 2062 2898 2899 3713 3993 2062 Size: 836 1 814 280 5390 Fragments arranged by size: 5390 836 814 280 1 EcoRI G'AATT_C Cuts at: 3044 3605 4630 5008 3044 Size: 561 1025 378 5357 Fragments arranged by size: 5357 1025 561 378 FspI TGC'GCA Cuts at: 3091 5827 3091 Size: 2736 4585 HaeII r_GCGC'y Cuts at: 1678 2188 2857 3253 3400 6468 1678 Size: 510 669 396 147 3068 2531 Fragments arranged by size: 3068 2531 669 510 396 147 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2264 2296 2972 3004 3113 Size: 32 135 32 692 32 676 32 109 Cuts at: 3113 3145 5962 5994 6036 6068 1373 Size: 32 2817 32 42 32 2626 Fragments arranged by size: 2817 2626 692 676 135 109 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3244 4665 4911 669 Size: 988 281 1306 1421 246 3079 Fragments arranged by size: 3079 1421 1306 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7321 HpaI GTT'AAC Cuts at: 3244 4911 3244 Size: 1667 5654 KasI G'GCGC_C Cuts at: 3249 3249 Size: 7321 KpnI G_GTAC'C Cuts at: 4646 4646 Size: 7321 MfeI C'AATT_G Cuts at: 4920 4920 Size: 7321 MluI A'CGCG_T Cuts at: 7197 7197 Size: 7321 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6435 6619 1305 Size: 5130 184 2007 Fragments arranged by size: 5130 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2459 2711 2882 3746 4046 Size: 54 1425 343 627 252 171 864 300 Cuts at: 4046 4545 10 Size: 499 2786 Fragments arranged by size: 2786 1425 864 627 499 343 300 252 171 54 MslI CAynn'nnrTG Cuts at: 518 1166 2394 2453 2705 2876 3143 3791 3893 Size: 648 1228 59 252 171 267 648 102 Cuts at: 3893 4176 4382 4388 5279 5638 5797 518 Size: 283 206 6 891 359 159 2042 Fragments arranged by size: 2042 1228 891 648 648 359 283 267 252 206 171 159 102 59 6 MspA1I CmG'CkG Cuts at: 1606 3066 3308 3690 4012 4075 4407 4737 5414 Size: 1460 242 382 322 63 332 330 677 Cuts at: 5414 6562 6807 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1460 1148 677 382 332 330 322 245 242 63 NaeI GCC'GGC Cuts at: 3481 3481 Size: 7321 NarI GG'CG_CC Cuts at: 3250 3250 Size: 7321 NcoI C'CATG_G Cuts at: 513 1065 2004 2389 2586 3165 3461 513 Size: 552 939 385 197 579 296 4373 Fragments arranged by size: 4373 939 579 552 385 296 197 NdeI CA'TA_TG Cuts at: 387 387 Size: 7321 NgoMIV G'CCGG_C Cuts at: 3479 3479 Size: 7321 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7321 NotI GC'GGCC_GC Cuts at: 4768 4768 Size: 7321 NspI r_CATG'y Cuts at: 1132 1229 2297 3190 4679 6224 1132 Size: 97 1068 893 1489 1545 2229 Fragments arranged by size: 2229 1545 1489 1068 893 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7321 PciI A'CATG_T Cuts at: 6220 6220 Size: 7321 PflMI CCAn_nnn'nTGG Cuts at: 1108 2188 3004 3415 1108 Size: 1080 816 411 5014 Fragments arranged by size: 5014 1080 816 411 PmeI GTTT'AAAC Cuts at: 4625 4625 Size: 7321 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3044 3076 4268 4300 5390 5422 6903 6935 3044 Size: 32 1192 32 1090 32 1481 32 3430 Fragments arranged by size: 3430 1481 1192 1090 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3713 3713 Size: 7321 PshAI GACnn'nnGTC Cuts at: 1613 2154 1613 Size: 541 6780 PsiI TTA'TAA Cuts at: 4891 4891 Size: 7321 PspOMI G'GGCC_C Cuts at: 2062 2898 3993 2062 Size: 836 1095 5390 Fragments arranged by size: 5390 1095 836 PstI C_TGCA'G Cuts at: 830 2781 3071 3506 4673 830 Size: 1951 290 435 1167 3478 Fragments arranged by size: 3478 1951 1167 435 290 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7321 PvuII CAG'CTG Cuts at: 3308 4075 3308 Size: 767 6554 SacI G_AGCT'C Cuts at: 3819 3819 Size: 7321 SalI G'TCGA_C Cuts at: 1936 4663 1936 Size: 2727 4594 SbfI CC_TGCA'GG Cuts at: 4673 4673 Size: 7321 ScaI AGT'ACT Cuts at: 5569 7281 5569 Size: 1712 5609 SfcI C'TryA_G Cuts at: 826 1045 2434 2777 3067 3502 4669 5804 6485 Size: 219 1389 343 290 435 1167 1135 681 Cuts at: 6485 6676 826 Size: 191 1471 Fragments arranged by size: 1471 1389 1167 1135 681 435 343 290 219 191 SfoI GGC'GCC Cuts at: 3251 3251 Size: 7321 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7321 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7321 SmaI CCC'GGG Cuts at: 1872 2831 3998 4648 1872 Size: 959 1167 650 4545 Fragments arranged by size: 4545 1167 959 650 SmlI C'TyrA_G Cuts at: 820 1017 1943 2467 5425 6326 6588 820 Size: 197 926 524 2958 901 262 1553 Fragments arranged by size: 2958 1553 926 901 524 262 197 SnaBI TAC'GTA Cuts at: 493 5112 493 Size: 4619 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7321 SphI G_CATG'C Cuts at: 1132 4679 1132 Size: 3547 3774 SspI AAT'ATT Cuts at: 5 52 5245 5 Size: 47 5193 2081 Fragments arranged by size: 5193 2081 47 StuI AGG'CCT Cuts at: 1517 2672 3803 1517 Size: 1155 1131 5035 Fragments arranged by size: 5035 1155 1131 StyI C'CwwG_G Cuts at: 513 1065 2004 2283 2389 2580 2586 3011 3165 Size: 552 939 279 106 191 6 425 154 Cuts at: 3165 3461 4540 4546 4760 513 Size: 296 1079 6 214 3074 Fragments arranged by size: 3074 1079 939 552 425 296 279 214 191 154 106 6 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2096 2246 2866 3329 3752 3824 4510 Size: 1255 516 150 620 463 423 72 686 Cuts at: 4510 5348 5365 5518 5703 6148 325 Size: 838 17 153 185 445 1498 Fragments arranged by size: 1498 1255 838 686 620 516 463 445 423 185 153 150 72 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3681 4302 5567 Size: 274 80 33 51 1443 1704 621 1265 Cuts at: 5567 7279 96 Size: 1712 138 Fragments arranged by size: 1712 1704 1443 1265 621 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2085 2117 2545 2577 7281 7313 2085 Size: 32 428 32 4704 32 2093 Fragments arranged by size: 4704 2093 428 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4609 1316 Size: 3293 4028 XbaI T'CTAG_A Cuts at: 4657 4657 Size: 7321 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2794 2794 Size: 7321 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7321 XmaI C'CCGG_G Cuts at: 1870 2829 3996 4646 1870 Size: 959 1167 650 4545 Fragments arranged by size: 4545 1167 959 650 XmnI GAAnn'nnTTC Cuts at: 5450 5450 Size: 7321 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5129 276 Size: 53 83 186 936 3595 2468 Fragments arranged by size: 3595 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI