Restriction map for
pFN21AE9522
(Linear) (Six-base) MAPSORT of: KIEE9522.seq Check: 3607 from: 1 to: 2616
KIEE9522.orf
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 239 2314 2616
Size: 239 2075 302
Fragments arranged by size:
2075 302 239
AleI CACnn'nnGTG
Cuts at: 0 871 1786 1888 2616
Size: 871 915 102 728
Fragments arranged by size:
915 871 728 102
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1039 1071 2616
Size: 1039 32 1545
Fragments arranged by size:
1545 1039 32
AlwNI CAG_nnn'CTG
Cuts at: 0 183 306 999 2616
Size: 183 123 693 1617
Fragments arranged by size:
1617 693 183 123
ApaI G_GGCC'C
Cuts at: 0 61 897 1992 2616
Size: 61 836 1095 624
Fragments arranged by size:
1095 836 624 61
ApaLI G'TGCA_C
Cuts at: 0 118 254 2616
Size: 118 136 2362
Fragments arranged by size:
2362 136 118
ApoI r'AATT_y
Cuts at: 0 1039 1600 2616
Size: 1039 561 1016
Fragments arranged by size:
1039 1016 561
AvaI C'yCGr_G
Cuts at: 0 824 1991 2061 2616
Size: 824 1167 70 555
Fragments arranged by size:
1167 824 555 70
BanI G'GyrC_C
Cuts at: 0 1244 1732 1999 2103 2487 2616
Size: 1244 488 267 104 384 129
Fragments arranged by size:
1244 488 384 267 129 104
BanII G_rGCy'C
Cuts at: 0 61 768 897 1508 1814 1992 2191 2616
Size: 61 707 129 611 306 178 199 425
Fragments arranged by size:
707 611 425 306 199 178 129 61
BbeI G_GCGC'C
Cuts at: 0 1248 2616
Size: 1248 1368
BbsI GAAGACnn'nnnn_
Cuts at: 0 1830 2616
Size: 1830 786
BbvCI CC'TCA_GC
Cuts at: 0 53 932 1470 2398 2616
Size: 53 879 538 928 218
Fragments arranged by size:
928 879 538 218 53
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2042 2076 2616
Size: 2042 34 540
Fragments arranged by size:
2042 540 34
BglI GCCn_nnn'nGGC
Cuts at: 0 368 793 1652 2402 2504 2616
Size: 368 425 859 750 102 112
Fragments arranged by size:
859 750 425 368 112 102
Bme1580I G_kGCm'C
Cuts at: 0 61 122 258 690 789 897 1112 1194
Size: 61 61 136 432 99 108 215 82
Cuts at: 1194 1737 1921 1992 2004 2113 2257 2490 2616
Size: 543 184 71 12 109 144 233 126
Fragments arranged by size:
543 432 233 215 184 144 136 126
109 108 99 82 71 61 61 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 109 776 1428 2550 2616
Size: 109 667 652 1122 66
Fragments arranged by size:
1122 667 652 109 66
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 975 2616
Size: 975 1641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 205 316 919 1498 2334 2514 2616
Size: 103 102 111 603 579 836 180 102
Fragments arranged by size:
836 603 579 180 111 103 102 102
Bpu10I CC'TnA_GC
Cuts at: 0 53 932 1470 2398 2616
Size: 53 879 538 928 218
Fragments arranged by size:
928 879 538 218 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 2616
Size: 483 2133
BsaHI Gr'CG_yC
Cuts at: 0 631 1245 1905 2616
Size: 631 614 660 711
Fragments arranged by size:
711 660 631 614
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 80 110 259 289 540 570 967 997
Size: 80 30 149 30 251 30 397 30
Cuts at: 997 1039 1069 2616
Size: 42 30 1547
Fragments arranged by size:
1547 397 251 149 80 42 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2200 2616
Size: 2200 416
BseYI C'CCAG_C
Cuts at: 0 60 99 135 1299 1685 1755 1827 2003
Size: 60 39 36 1164 386 70 72 176
Cuts at: 2003 2616
Size: 613
Fragments arranged by size:
1164 613 386 176 72 70 60 39
36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1400 1555 2208 2425 2616
Size: 1400 155 653 217 191
Fragments arranged by size:
1400 653 217 191 155
BsiHKAI G_wGCw'C
Cuts at: 0 122 167 258 1271 1814 2210 2616
Size: 122 45 91 1013 543 396 406
Fragments arranged by size:
1013 543 406 396 122 91 45
BsmI GAATG_Cn'
Cuts at: 0 1565 2323 2616
Size: 1565 758 293
Fragments arranged by size:
1565 758 293
Bsp1286I G_dGCh'C
Cuts at: 0 61 122 167 258 690 768 789 897
Size: 61 61 45 91 432 78 21 108
Cuts at: 897 1112 1194 1271 1508 1737 1814 1921 1992
Size: 215 82 77 237 229 77 107 71
Cuts at: 1992 2004 2113 2191 2210 2257 2490 2616
Size: 12 109 78 19 47 233 126
Fragments arranged by size:
432 237 233 229 215 126 109 108
107 91 82 78 78 77 77 71
61 61 47 45 21 19 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 239 2314 2616
Size: 239 2075 302
Fragments arranged by size:
2075 302 239
BsrDI GCAATG_nn'
Cuts at: 0 1868 1967 2583 2616
Size: 1868 99 616 33
Fragments arranged by size:
1868 616 99 33
BsrFI r'CCGG_y
Cuts at: 0 1474 2616
Size: 1474 1142
BsrGI T'GTAC_A
Cuts at: 0 2297 2616
Size: 2297 319
BssSI C'ACGA_G
Cuts at: 0 683 2616
Size: 683 1933
BstAPI GCAn_nnn'nTGC
Cuts at: 0 234 1571 2616
Size: 234 1337 1045
Fragments arranged by size:
1337 1045 234
BstBI TT'CG_AA
Cuts at: 0 1661 2616
Size: 1661 955
BstXI CCAn_nnnn'nTGG
Cuts at: 0 450 582 954 1788 2049 2548 2616
Size: 450 132 372 834 261 499 68
Fragments arranged by size:
834 499 450 372 261 132 68
BstYI r'GATC_y
Cuts at: 0 476 2616
Size: 476 2140
Bsu36I CC'TnA_GG
Cuts at: 0 1800 2616
Size: 1800 816
BtgI C'CryG_G
Cuts at: 0 168 333 384 581 1160 1456 1883 2408
Size: 168 165 51 197 579 296 427 525
Cuts at: 2408 2616
Size: 208
Fragments arranged by size:
579 525 427 296 208 197 168 165
51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1240 1704 1706 2186 2199 2594 2616
Size: 1240 464 2 480 13 395 22
Fragments arranged by size:
1240 480 464 395 22 13 2
BtsI GCAGTG_nn'
Cuts at: 0 25 398 924 1157 1392 1506 1895 1919
Size: 25 373 526 233 235 114 389 24
Cuts at: 1919 2152 2514 2616
Size: 233 362 102
Fragments arranged by size:
526 389 373 362 235 233 233 114
102 25 24
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1731 1766 2616
Size: 1731 35 850
Fragments arranged by size:
1731 850 35
DraIII CAC_nnn'GTG
Cuts at: 0 2381 2616
Size: 2381 235
DrdI GACnn_nn'nnGTC
Cuts at: 0 618 2616
Size: 618 1998
EaeI y'GGCC_r
Cuts at: 0 452 704 875 1231 1350 1739 2008 2039
Size: 452 252 171 356 119 389 269 31
Cuts at: 2039 2538 2580 2616
Size: 499 42 36
Fragments arranged by size:
499 452 389 356 269 252 171 119
42 36 31
EarI CTCTTCn'nnn_
Cuts at: 0 1522 2458 2616
Size: 1522 936 158
Fragments arranged by size:
1522 936 158
EcoICRI GAG'CTC
Cuts at: 0 1812 2616
Size: 1812 804
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 205 316 919 1498 2334 2514 2616
Size: 103 102 111 603 579 836 180 102
Fragments arranged by size:
836 603 579 180 111 103 102 102
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1255 2616
Size: 1255 1361
EcoO109I rG'GnC_Cy
Cuts at: 0 57 893 894 1708 1988 2616
Size: 57 836 1 814 280 628
Fragments arranged by size:
836 814 628 280 57 1
EcoRI G'AATT_C
Cuts at: 0 1039 1600 2616
Size: 1039 561 1016
Fragments arranged by size:
1039 1016 561
FspI TGC'GCA
Cuts at: 0 1086 2616
Size: 1086 1530
HaeII r_GCGC'y
Cuts at: 0 183 852 1248 1395 2616
Size: 183 669 396 147 1221
Fragments arranged by size:
1221 669 396 183 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 967 999 1108 1140 2616
Size: 259 32 676 32 109 32 1476
Fragments arranged by size:
1476 676 259 109 32 32 32
HincII GTy'rAC
Cuts at: 0 1239 2616
Size: 1239 1377
HpaI GTT'AAC
Cuts at: 0 1239 2616
Size: 1239 1377
KasI G'GCGC_C
Cuts at: 0 1244 2616
Size: 1244 1372
MscI TGG'CCA
Cuts at: 0 454 706 877 1741 2041 2540 2616
Size: 454 252 171 864 300 499 76
Fragments arranged by size:
864 499 454 300 252 171 76
MslI CAynn'nnrTG
Cuts at: 0 389 448 700 871 1138 1786 1888 2171
Size: 389 59 252 171 267 648 102 283
Cuts at: 2171 2377 2383 2616
Size: 206 6 233
Fragments arranged by size:
648 389 283 267 252 233 206 171
102 59 6
MspA1I CmG'CkG
Cuts at: 0 1061 1303 1685 2007 2070 2402 2616
Size: 1061 242 382 322 63 332 214
Fragments arranged by size:
1061 382 332 322 242 214 63
NaeI GCC'GGC
Cuts at: 0 1476 2616
Size: 1476 1140
NarI GG'CG_CC
Cuts at: 0 1245 2616
Size: 1245 1371
NcoI C'CATG_G
Cuts at: 0 384 581 1160 1456 2616
Size: 384 197 579 296 1160
Fragments arranged by size:
1160 579 384 296 197
NgoMIV G'CCGG_C
Cuts at: 0 1474 2616
Size: 1474 1142
NspI r_CATG'y
Cuts at: 0 292 1185 2616
Size: 292 893 1431
Fragments arranged by size:
1431 893 292
PflMI CCAn_nnn'nTGG
Cuts at: 0 183 999 1410 2616
Size: 183 816 411 1206
Fragments arranged by size:
1206 816 411 183
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1039 1071 2263 2295 2616
Size: 1039 32 1192 32 321
Fragments arranged by size:
1192 1039 321 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1708 2616
Size: 1708 908
PshAI GACnn'nnGTC
Cuts at: 0 149 2616
Size: 149 2467
PspOMI G'GGCC_C
Cuts at: 0 57 893 1988 2616
Size: 57 836 1095 628
Fragments arranged by size:
1095 836 628 57
PstI C_TGCA'G
Cuts at: 0 776 1066 1501 2616
Size: 776 290 435 1115
Fragments arranged by size:
1115 776 435 290
PvuII CAG'CTG
Cuts at: 0 1303 2070 2616
Size: 1303 767 546
Fragments arranged by size:
1303 767 546
SacI G_AGCT'C
Cuts at: 0 1814 2616
Size: 1814 802
SfcI C'TryA_G
Cuts at: 0 429 772 1062 1497 2616
Size: 429 343 290 435 1119
Fragments arranged by size:
1119 435 429 343 290
SfoI GGC'GCC
Cuts at: 0 1246 2616
Size: 1246 1370
SmaI CCC'GGG
Cuts at: 0 826 1993 2616
Size: 826 1167 623
Fragments arranged by size:
1167 826 623
SmlI C'TyrA_G
Cuts at: 0 462 2616
Size: 462 2154
StuI AGG'CCT
Cuts at: 0 667 1798 2616
Size: 667 1131 818
Fragments arranged by size:
1131 818 667
StyI C'CwwG_G
Cuts at: 0 278 384 575 581 1006 1160 1456 2535
Size: 278 106 191 6 425 154 296 1079
Cuts at: 2535 2541 2616
Size: 6 75
Fragments arranged by size:
1079 425 296 278 191 154 106 75
6 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 91 241 861 1324 1747 1819 2505 2616
Size: 91 150 620 463 423 72 686 111
Fragments arranged by size:
686 620 463 423 150 111 91 72
TatI w'GTAC_w
Cuts at: 0 1676 2297 2616
Size: 1676 621 319
Fragments arranged by size:
1676 621 319
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 80 112 540 572 2616
Size: 80 32 428 32 2044
Fragments arranged by size:
2044 428 80 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2604 2616
Size: 2604 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 789 2616
Size: 789 1827
XmaI C'CCGG_G
Cuts at: 0 824 1991 2616
Size: 824 1167 625
Fragments arranged by size:
1167 824 625
Enzymes that do cut:
AarI AleI AloI AlwNI ApaI ApaLI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BglI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HpaI KasI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PflMI PpiI PpuMI PshAI PspOMI PstI
PvuII SacI SfcI SfoI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AscI AseI AsiSI AvrII BaeI
BamHI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BssHII BstEII BstZ17I ClaI DraI EagI
EciI EcoRV FalI FseI FspAI HindIII KpnI MfeI
MluI MmeI NdeI NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XhoI XmnI ZraI