Restriction map for pFN21AE3426
 (Linear) (Six-base) MAPSORT of: KIEE3426.seq  Check: 2304  from: 1  to: 1779



KIEE3426.orf

 With 182 enzymes: SgfI * 

                        October 12, 2011 16:54  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1665    1752    1779 
   Size:       1665      87      27

  Fragments arranged by size: 

                1665      87      27
 
AatII G_ACGT'C

Cuts at:      0      92    1779 
   Size:         92    1687
 
Acc65I G'GTAC_C

Cuts at:      0     305    1779 
   Size:        305    1474
 
AleI CACnn'nnGTG

Cuts at:      0    1231    1508    1510    1779 
   Size:       1231     277       2     269

  Fragments arranged by size: 

                1231     277     269       2
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      98     130    1669    1701    1779 
   Size:         98      32    1539      32      78

  Fragments arranged by size: 

                1539      98      78      32      32
 
ApoI r'AATT_y

Cuts at:      0     514    1779 
   Size:        514    1265
 
AvaI C'yCGr_G

Cuts at:      0    1642    1779 
   Size:       1642     137
 
BanI G'GyrC_C

Cuts at:      0     288     305     314     363     468     739     798    1092 
   Size:        288      17       9      49     105     271      59     294

Cuts at:   1092    1779 
   Size:        687

  Fragments arranged by size: 

                 687     294     288     271     105      59      49      17
                   9
 
BanII G_rGCy'C

Cuts at:      0    1066    1779 
   Size:       1066     713
 
BbeI G_GCGC'C

Cuts at:      0     318     743     802    1779 
   Size:        318     425      59     977

  Fragments arranged by size: 

                 977     425     318      59
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     969     993    1260    1779 
   Size:        969      24     267     519

  Fragments arranged by size: 

                 969     519     267      24
 
BbvCI CC'TCA_GC

Cuts at:      0    1552    1595    1779 
   Size:       1552      43     184

  Fragments arranged by size: 

                1552     184      43
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1737    1779 
   Size:       1737      42
 
BglII A'GATC_T

Cuts at:      0     110    1779 
   Size:        110    1669
 
BlpI GC'TnA_GC

Cuts at:      0      55     758    1779 
   Size:         55     703    1021

  Fragments arranged by size: 

                1021     703      55
 
Bme1580I G_kGCm'C

Cuts at:      0     366     389     479    1097    1779 
   Size:        366      23      90     618     682

  Fragments arranged by size: 

                 682     618     366      90      23
 
BmgBI CAC'GTC

Cuts at:      0     488    1779 
   Size:        488    1291
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     132    1779 
   Size:        132    1647
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1740    1779 
   Size:       1740      39
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1654    1779 
   Size:       1654     125
 
Bpu10I CC'TnA_GC

Cuts at:      0     131    1552    1595    1704    1733    1779 
   Size:        131    1421      43     109      29      46

  Fragments arranged by size: 

                1421     131     109      46      43      29
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139    1080    1779 
   Size:        139     941     699

  Fragments arranged by size: 

                 941     699     139
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1165    1299    1630    1639    1779 
   Size:       1165     134     331       9     140

  Fragments arranged by size: 

                1165     331     140     134       9
 
BsaBI GATnn'nnATC

Cuts at:      0     207    1779 
   Size:        207    1572
 
BsaHI Gr'CG_yC

Cuts at:      0      89     315     598     740     799    1253    1779 
   Size:         89     226     283     142      59     454     526

  Fragments arranged by size: 

                 526     454     283     226     142      89      59
 
BsaWI w'CCGG_w

Cuts at:      0     393    1208    1779 
   Size:        393     815     571

  Fragments arranged by size: 

                 815     571     393
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     895     925    1048    1078    1779 
   Size:        895      30     123      30     701

  Fragments arranged by size: 

                 895     701     123      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     289     787     790    1732    1779 
   Size:        289     498       3     942      47

  Fragments arranged by size: 

                 942     498     289      47       3
 
BseYI C'CCAG_C

Cuts at:      0     357     458     983    1052    1626    1779 
   Size:        357     101     525      69     574     153

  Fragments arranged by size: 

                 574     525     357     153     101      69
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     628     633     901    1779 
   Size:        106     522       5     268     878

  Fragments arranged by size: 

                 878     522     268     106       5
 
BsiEI CG_ry'CG

Cuts at:      0    1185    1779 
   Size:       1185     594
 
BsiHKAI G_wGCw'C

Cuts at:      0      56     132     620     731    1066    1118    1154    1238 
   Size:         56      76     488     111     335      52      36      84

Cuts at:   1238    1779 
   Size:        541

  Fragments arranged by size: 

                 541     488     335     111      84      76      56      52
                  36
 
BsiWI C'GTAC_G

Cuts at:      0      65    1779 
   Size:         65    1714
 
Bsp1286I G_dGCh'C

Cuts at:      0      56     132     366     389     479     620     731    1066 
   Size:         56      76     234      23      90     141     111     335

Cuts at:   1066    1097    1118    1154    1238    1779 
   Size:         31      21      36      84     541

  Fragments arranged by size: 

                 541     335     234     141     111      90      84      76
                  56      36      31      23      21
 
BspEI T'CCGG_A

Cuts at:      0     393    1779 
   Size:        393    1386
 
BspHI T'CATG_A

Cuts at:      0      92    1779 
   Size:         92    1687
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     293     923    1665    1752    1779 
   Size:        293     630     742      87      27

  Fragments arranged by size: 

                 742     630     293      87      27
 
BsrBI CCG'CTC

Cuts at:      0    1737    1779 
   Size:       1737      42
 
BsrDI GCAATG_nn'

Cuts at:      0     456    1779 
   Size:        456    1323
 
BsrFI r'CCGG_y

Cuts at:      0     826    1180    1184    1779 
   Size:        826     354       4     595

  Fragments arranged by size: 

                 826     595     354       4
 
BssHII G'CGCG_C

Cuts at:      0     696     783    1779 
   Size:        696      87     996

  Fragments arranged by size: 

                 996     696      87
 
BssSI C'ACGA_G

Cuts at:      0     533    1779 
   Size:        533    1246
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     186    1779 
   Size:        186    1593
 
BstYI r'GATC_y

Cuts at:      0     110    1779 
   Size:        110    1669
 
BtgI C'CryG_G

Cuts at:      0     575    1493    1661    1779 
   Size:        575     918     168     118

  Fragments arranged by size: 

                 918     575     168     118
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     774    1779 
   Size:        774    1005
 
BtsI GCAGTG_nn'

Cuts at:      0     645    1571    1779 
   Size:        645     926     208

  Fragments arranged by size: 

                 926     645     208
 
DraIII CAC_nnn'GTG

Cuts at:      0     370    1049    1779 
   Size:        370     679     730

  Fragments arranged by size: 

                 730     679     370
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     213    1779 
   Size:        213    1566
 
EaeI y'GGCC_r

Cuts at:      0     352     875    1037    1182    1274    1587    1605    1738 
   Size:        352     523     162     145      92     313      18     133

Cuts at:   1738    1779 
   Size:         41

  Fragments arranged by size: 

                 523     352     313     162     145     133      92      41
                  18
 
EagI C'GGCC_G

Cuts at:      0    1182    1779 
   Size:       1182     597
 
EarI CTCTTCn'nnn_

Cuts at:      0    1135    1342    1754    1779 
   Size:       1135     207     412      25

  Fragments arranged by size: 

                1135     412     207      25
 
EcoICRI GAG'CTC

Cuts at:      0    1064    1779 
   Size:       1064     715
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1654    1779 
   Size:       1654     125
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     633     769    1714    1753    1779 
   Size:        633     136     945      39      26

  Fragments arranged by size: 

                 945     633     136      39      26
 
EcoO109I rG'GnC_Cy

Cuts at:      0     162    1622    1779 
   Size:        162    1460     157

  Fragments arranged by size: 

                1460     162     157
 
HaeII r_GCGC'y

Cuts at:      0      34     318     743     765     802    1779 
   Size:         34     284     425      22      37     977

  Fragments arranged by size: 

                 977     425     284      37      34      22
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     232     264    1048    1080    1346    1378    1779 
   Size:        232      32     784      32     266      32     401

  Fragments arranged by size: 

                 784     401     266     232      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     314     739     798    1779 
   Size:        314     425      59     981

  Fragments arranged by size: 

                 981     425     314      59
 
KpnI G_GTAC'C

Cuts at:      0     309    1779 
   Size:        309    1470
 
MscI TGG'CCA

Cuts at:      0     877    1039    1276    1607    1779 
   Size:        877     162     237     331     172

  Fragments arranged by size: 

                 877     331     237     172     162
 
MslI CAynn'nnrTG

Cuts at:      0     667     931    1090    1231    1508    1510    1779 
   Size:        667     264     159     141     277       2     269

  Fragments arranged by size: 

                 667     277     269     264     159     141       2
 
MspA1I CmG'CkG

Cuts at:      0     357     458     823     997    1427    1466    1599    1779 
   Size:        357     101     365     174     430      39     133     180

  Fragments arranged by size: 

                 430     365     357     180     174     133     101      39
            
 
NaeI GCC'GGC

Cuts at:      0     828    1182    1779 
   Size:        828     354     597

  Fragments arranged by size: 

                 828     597     354
 
NarI GG'CG_CC

Cuts at:      0     315     740     799    1779 
   Size:        315     425      59     980

  Fragments arranged by size: 

                 980     425     315      59
 
NcoI C'CATG_G

Cuts at:      0    1493    1661    1779 
   Size:       1493     168     118

  Fragments arranged by size: 

                1493     168     118
 
NgoMIV G'CCGG_C

Cuts at:      0     826    1180    1779 
   Size:        826     354     599

  Fragments arranged by size: 

                 826     599     354
 
NspI r_CATG'y

Cuts at:      0     672    1779 
   Size:        672    1107
 
PfoI T'CCnGG_A

Cuts at:      0    1066    1779 
   Size:       1066     713
 
PpuMI rG'GwC_Cy

Cuts at:      0     162    1622    1779 
   Size:        162    1460     157

  Fragments arranged by size: 

                1460     162     157
 
PshAI GACnn'nnGTC

Cuts at:      0    1519    1779 
   Size:       1519     260
 
PstI C_TGCA'G

Cuts at:      0     170     844    1779 
   Size:        170     674     935

  Fragments arranged by size: 

                 935     674     170
 
PvuII CAG'CTG

Cuts at:      0     458    1599    1779 
   Size:        458    1141     180

  Fragments arranged by size: 

                1141     458     180
 
SacI G_AGCT'C

Cuts at:      0    1066    1779 
   Size:       1066     713
 
SanDI GG'GwC_CC

Cuts at:      0    1622    1779 
   Size:       1622     157
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1342    1779 
   Size:       1342     437
 
SexAI A'CCwGG_T

Cuts at:      0     366    1779 
   Size:        366    1413
 
SfcI C'TryA_G

Cuts at:      0     166     498     840    1479    1753    1779 
   Size:        166     332     342     639     274      26

  Fragments arranged by size: 

                 639     342     332     274     166      26
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1737    1779 
   Size:       1737      42
 
SfoI GGC'GCC

Cuts at:      0     316     741     800    1779 
   Size:        316     425      59     979

  Fragments arranged by size: 

                 979     425     316      59
 
SmlI C'TyrA_G

Cuts at:      0     154    1059    1642    1779 
   Size:        154     905     583     137

  Fragments arranged by size: 

                 905     583     154     137
 
StuI AGG'CCT

Cuts at:      0    1709    1723    1779 
   Size:       1709      14      56

  Fragments arranged by size: 

                1709      56      14
 
StyI C'CwwG_G

Cuts at:      0    1493    1661    1673    1779 
   Size:       1493     168      12     106

  Fragments arranged by size: 

                1493     168     106      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     306    1779 
   Size:        306    1473
 
XhoI C'TCGA_G

Cuts at:      0    1642    1779 
   Size:       1642     137
 
ZraI GAC'GTC

Cuts at:      0      90    1779 
   Size:         90    1689
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AleI     AloI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI    BbvCI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI    BstXI    BstYI     BtgI    BtgZI     BtsI   DraIII
     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    HaeII    Hin4I     KasI     KpnI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PfoI    PpuMI    PshAI     PstI
    PvuII     SacI    SanDI     SapI    SexAI     SfcI     SfiI     SfoI
     SmlI     StuI     StyI    TaqII     XhoI     ZraI

 Enzymes that do not cut: 

     AccI     AclI     AcuI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BcgI    BciVI     BclI    BfrBI     BmtI    BsaAI
     BsmI    BsmBI    BsrGI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     ClaI    CspCI     DraI     EciI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     MfeI     MluI     MmeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI     SbfI     ScaI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI
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