################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BbeI BbvCI BciVI BclI BcnI BfaI BfrI BglII BlnI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MaeIII MfeI MflI MluI MroI MseI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AocI 94 CCTAAGG CC^T[ACGT]AGG BalI 470 TGGCCA TGG^CCA BanI 382 GGCACC G^G[CT][GA]CC BbrPI 463 CACGTG CAC^GTG BcgI 105 CGAGTTTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 46 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 451 GCTGAGC GC^T[ACGT]AGC Bpu1102I 451 GCTGAGC GC^T[ACGT]AGC BsaAI 463 CACGTG [CT]AC^GT[GA] BsaBI 204 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 183 CGACCG CG[GA][CT]^CG BsrDI 431 GCAATG GCAATG(2/0) BssKI 285 CCTGG ^CC[ACGT]GG BstNI 285 CCTGG CC^[AT]GG BstXI 317 CCAAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 94 CCTAAGG CC^T[ACGT]AGG CelII 451 GCTGAGC GC^T[ACGT]AGC EaeI 470 TGGCCA [CT]^GGCC[GA] Eco81I 94 CCTAAGG CC^T[ACGT]AGG EcoRII 285 CCTGG ^CC[AT]GG HinfI 532 GACTC G^A[ACGT]TC MaeII 464 ACGT A^CGT MamI 204 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 470 TGGCCA TGG^CCA MscI 470 TGGCCA TGG^CCA MvaI 285 CCTGG CC^[AT]GG PflMI 462 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 532 GACTC GAGTC(4/5) PmaCI 463 CACGTG CAC^GTG PmlI 463 CACGTG CAC^GTG SacI 307 GAGCTC GAGCT^C SapI 304 GAAGAGC GCTCTTC(1/4) ScrFI 285 CCTGG CC^[ACGT]GG Van91I 462 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AspHI 274 GTGCTC G[AT]GC[AT]^C 307 GAGCTC BanII 19 GAGCCC G[GA]GC[CT]^C 307 GAGCTC BbsI 496 GAAGAC GAAGAC(2/6) 415 GTCTTC BpmI 59 CTCCAG CTGGAG(16/14) 352 CTCCAG BpuAI 496 GAAGAC GAAGAC(2/6) 415 GTCTTC BsiHKAI 274 GTGCTC G[AT]GC[AT]^C 307 GAGCTC BstF5I 30 GGATG GGATG(2/0) 231 GGATG Cac8I 240 GCGGGC GC[ACGT]^[ACGT]GC 468 GCTGGC CfoI 90 GCGC GCG^C 244 GCGC Cfr13I 3 GGCCC G^G[ACGT]CC 249 GGGCC DpnII 123 GATC ^GATC 210 GATC EarI 154 GAAGAG CTCTTC(1/4) 304 GAAGAG EcoT14I 38 CCAAGG C^C[AT][AT]GG 50 CCAAGG FokI 30 GGATG GGATG(9/13) 231 GGATG HaeII 89 AGCGCC [GA]GCGC^[CT] 243 GGCGCT HhaI 90 GCGC GCG^C 244 GCGC HinP1I 90 GCGC G^CGC 244 GCGC HphI 481 GGTGA GGTGA(8/7) 212 TCACC Ksp632I 154 GAAGAG CTCTTC(1/4) 304 GAAGAG MboI 123 GATC ^GATC 210 GATC MslI 191 CACCCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 438 CACTGTCATG NdeII 123 GATC ^GATC 210 GATC NlaIV 3 GGCCCC GG[ACGT]^[ACGT]CC 382 GGCACC Sau3AI 123 GATC ^GATC 210 GATC Sau96I 3 GGCCC G^G[ACGT]CC 249 GGGCC StyI 38 CCAAGG C^C[AT][AT]GG 50 CCAAGG TspRI 223 CAGTG CA[GC]TG[ACGT][ACGT]^ 438 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AciI AluI BbvI BmyI BsaJI BsiYI BslI Bsp1286I DdeI FauI Fnu4HI HaeIII ItaI MboII MnlI MwoI NlaIII SfaNI TaqI TseI TthHB8I