(Linear) (Six-base) MAPSORT of: KIEE6518.seq Check: 7446 from: 1 to: 585 KIEE6518.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AleI CACnn'nnGTG Cuts at: 0 195 585 Size: 195 390 BanI G'GyrC_C Cuts at: 0 382 585 Size: 382 203 BanII G_rGCy'C Cuts at: 0 23 311 585 Size: 23 288 274 Fragments arranged by size: 288 274 23 BbsI GAAGACnn'nnnn_ Cuts at: 0 408 503 585 Size: 408 95 82 Fragments arranged by size: 408 95 82 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 94 128 585 Size: 94 34 457 Fragments arranged by size: 457 94 34 BglI GCCn_nnn'nGGC Cuts at: 0 52 585 Size: 52 533 BlpI GC'TnA_GC Cuts at: 0 452 585 Size: 452 133 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 44 337 585 Size: 44 293 248 Fragments arranged by size: 293 248 44 Bpu10I CC'TnA_GC Cuts at: 0 80 86 143 585 Size: 80 6 57 442 Fragments arranged by size: 442 80 57 6 BsaAI yAC'GTr Cuts at: 0 465 585 Size: 465 120 BsaBI GATnn'nnATC Cuts at: 0 208 585 Size: 208 377 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 91 121 585 Size: 91 30 464 Fragments arranged by size: 464 91 30 BseYI C'CCAG_C Cuts at: 0 246 477 520 585 Size: 246 231 43 65 Fragments arranged by size: 246 231 65 43 BsiEI CG_ry'CG Cuts at: 0 186 585 Size: 186 399 BsiHKAI G_wGCw'C Cuts at: 0 278 311 585 Size: 278 33 274 Fragments arranged by size: 278 274 33 Bsp1286I G_dGCh'C Cuts at: 0 23 278 311 585 Size: 23 255 33 274 Fragments arranged by size: 274 255 33 23 BsrDI GCAATG_nn' Cuts at: 0 438 585 Size: 438 147 BstXI CCAn_nnnn'nTGG Cuts at: 0 324 585 Size: 324 261 Bsu36I CC'TnA_GG Cuts at: 0 95 585 Size: 95 490 BtsI GCAGTG_nn' Cuts at: 0 229 585 Size: 229 356 EaeI y'GGCC_r Cuts at: 0 470 585 Size: 470 115 EarI CTCTTCn'nnn_ Cuts at: 0 149 299 585 Size: 149 150 286 Fragments arranged by size: 286 150 149 EcoICRI GAG'CTC Cuts at: 0 309 585 Size: 309 276 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 44 337 585 Size: 44 293 248 Fragments arranged by size: 293 248 44 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 186 585 Size: 186 399 HaeII r_GCGC'y Cuts at: 0 93 247 585 Size: 93 154 338 Fragments arranged by size: 338 154 93 MscI TGG'CCA Cuts at: 0 472 585 Size: 472 113 MslI CAynn'nnrTG Cuts at: 0 195 442 585 Size: 195 247 143 Fragments arranged by size: 247 195 143 PflMI CCAn_nnn'nTGG Cuts at: 0 468 585 Size: 468 117 PmlI CAC'GTG Cuts at: 0 465 585 Size: 465 120 SacI G_AGCT'C Cuts at: 0 311 585 Size: 311 274 SapI GCTCTTCn'nnn_ Cuts at: 0 299 585 Size: 299 286 StyI C'CwwG_G Cuts at: 0 38 50 585 Size: 38 12 535 Fragments arranged by size: 535 38 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 207 436 469 585 Size: 207 229 33 116 Fragments arranged by size: 229 207 116 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 562 585 Size: 562 23 Enzymes that do cut: AleI BanI BanII BbsI BcgI BglI BlpI BpmI Bpu10I BsaAI BsaBI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BsrDI BstXI Bsu36I BtsI EaeI EarI EcoICRI Eco57MI FalI HaeII MscI MslI PflMI PmlI SacI SapI StyI TaqII TstI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglII Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaI BsaHI BsaWI BseRI BsgI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI