################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII Acc65I AflIII AgeI Aor51HI AscI AseI AsnI Asp718I AviII AvrII BbrPI BcgI BciVI BlnI BsaAI BsaBI BsiEI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoT22I FseI FspI HpaI KpnI KspI MamI MfeI MluI MspA1I MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI PvuI PvuII RsrII SacII SalI SapI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 127 GTCTAC GT^[AC][GT]AC AccII 2210 CGCG CG^CG AccIII 597 TCCGGA T^CCGGA AflII 2250 CTTAAG C^TTAAG AhdI 745 GACGACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2786 GTGCAC G^TGCAC AlwNI 2677 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1677 CCTCAGG CC^T[ACGT]AGG ApaI 1239 GGGCCC GGGCC^C ApaLI 2786 GTGCAC G^TGCAC AspEI 745 GACGACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 852 TGGCCA TGG^CCA BamHI 2883 GGATCC G^GATCC BbeI 24 GGCGCC GGCGC^C BbvCI 2701 CCTCAGC CCTCAGC(-5/-2) BclI 1871 TGATCA T^GATCA BfrI 2250 CTTAAG C^TTAAG BglI 100 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1284 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1284 GCTGAGC GC^T[ACGT]AGC BsaWI 597 TCCGGA [AT]^CCGG[AT] BseAI 597 TCCGGA T^CCGGA BspEI 597 TCCGGA T^CCGGA BspMI 530 ACCTGC ACCTGC(4/8) BsrBI 1268 CCGCTC CCGCTC(-3/-3) BsrFI 3 GCCGGT [GA]^CCGG[CT] BssSI 976 CTCGTG CACGAG(-5/-1) BstUI 2210 CGCG CG^CG Bsu36I 1677 CCTCAGG CC^T[ACGT]AGG CelII 1284 GCTGAGC GC^T[ACGT]AGC Cfr10I 3 GCCGGT [GA]^CCGG[CT] DsaI 1784 CCATGG C^C[GA][CT]GG EaeI 852 TGGCCA [CT]^GGCC[GA] Eam1105I 745 GACGACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1677 CCTCAGG CC^T[ACGT]AGG EcoRI 274 GAATTC G^AATTC EcoRV 2043 GATATC GAT^ATC FauI 2819 GCGGG CCCGC(4/6) FbaI 1871 TGATCA T^GATCA HaeII 24 GGCGCC [GA]GCGC^[CT] HindIII 2253 AAGCTT A^AGCTT KasI 24 GGCGCC G^GCGCC MluNI 852 TGGCCA TGG^CCA MroI 597 TCCGGA T^CCGGA MscI 852 TGGCCA TGG^CCA MvnI 2210 CGCG CG^CG NarI 24 GGCGCC GG^CGCC NcoI 1784 CCATGG C^CATGG PpuMI 469 GGGACCC [GA]G^G[AT]CC[CT] PshAI 2422 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 2921 GAGCTC GAGCT^C SfoI 24 GGCGCC GGC^GCC SspI 1526 AATATT AAT^ATT XcmI 95 CCAATGCCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcyI 24 GGCGCC G[GA]^CG[CT]C 3060 GACGCC AspI 442 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 494 GACGGAGTC AvaI 1776 CTCGGG C^[CT]CG[GA]G 2271 CTCGGG BglII 1343 AGATCT A^GATCT 1634 AGATCT BsaHI 24 GGCGCC G[GA]^CG[CT]C 3060 GACGCC BsgI 1356 CTGCAC GTGCAG(16/14) 2190 CTGCAC BsmBI 1317 CGTCTC CGTCTC(1/5) 492 GAGACG BsoBI 1776 CTCGGG C^[CT]CG[GA]G 2271 CTCGGG BsrDI 783 CATTGC GCAATG(2/0) 740 GCAATG BstXI 2910 CCAACGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3205 CCAGGATCTTGG DraI 658 TTTAAA TTT^AAA 819 TTTAAA DraII 469 GGGACCC [GA]G^G[ACGT]CC[CT] 1238 GGGGCCC EcoO109I 469 GGGACCC [GA]G^G[ACGT]CC[CT] 1238 GGGGCCC Hin1I 24 GGCGCC G[GA]^CG[CT]C 3060 GACGCC HincII 1549 GTCAAC GT[CT]^[GA]AC 3057 GTTGAC HindII 1549 GTCAAC GT[CT]^[GA]AC 3057 GTTGAC PflFI 442 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 494 GACGGAGTC ScaI 369 AGTACT AGT^ACT 1054 AGTACT SmlI 1507 CTCAAG C^T[CT][GA]AG 2250 CTTAAG Tth111I 442 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 494 GACGGAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AciI AclI AcsI AfaI AluI AlwI ApoI Asp700I AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI Psp1406I PstI RcaI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI