################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoT14I FauI FbaI FseI FspI HaeII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI KspI MaeI MaeIII MfeI MluI MluNI MroI MscI MspA1I MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tsp45I Tsp509I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 399 CGCG CG^CG AcyI 241 GGCGTC G[GA]^CG[CT]C AfaI 207 GTAC GT^AC AlwI 339 GATCC GGATC(4/5) AlwNI 137 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 13 GAGCAC G[AT]GC[AT]^C AspI 103 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 25 CTCGAG C^[CT]CG[GA]G BbsI 124 GAAGAC GAAGAC(2/6) BglII 21 AGATCT A^GATCT BpuAI 124 GAAGAC GAAGAC(2/6) BsaBI 333 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 241 GGCGTC G[GA]^CG[CT]C BsaI 80 GAGACC GGTCTC(1/5) BsiHKAI 13 GAGCAC G[AT]GC[AT]^C BsmFI 244 GTCCC GGGAC(10/14) BsoBI 25 CTCGAG C^[CT]CG[GA]G BstAPI 377 GCACCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 372 CATCC GGATG(2/0) BstUI 399 CGCG CG^CG BstXI 230 CCACGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 277 GCCTGC GC[ACGT]^[ACGT]GC EcoNI 405 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 46 GATATC GAT^ATC EcoT22I 315 ATGCAT ATGCA^T FokI 372 CATCC GGATG(9/13) HgaI 242 GCGTC GACGC(5/10) Hin1I 241 GGCGTC G[GA]^CG[CT]C MaeII 128 ACGT A^CGT MamI 333 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 426 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 399 CGCG CG^CG NlaIII 314 CATG CATG^ NsiI 315 ATGCAT ATGCA^T PaeR7I 25 CTCGAG C^TCGAG PflFI 103 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 203 CCAAGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 417 CTGCAG CTGCA^G RsaI 207 GTAC GT^AC SapI 10 GAAGAGC GCTCTTC(1/4) ScaI 206 AGTACT AGT^ACT SexAI 68 ACCTGGT A^CC[AT]GGT SmlI 25 CTCGAG C^T[CT][GA]AG Tth111I 103 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 203 CCAAGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 25 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BanI 148 GGCACC G^G[CT][GA]CC 309 GGTGCC BmyI 13 GAGCAC G[AGT]GC[ACT]^C 147 GGGCAC BsaJI 404 CCCTGG C^C[ACGT][ACGT]GG 405 CCTGGG BsmAI 80 GAGAC GTCTC(1/5) 199 GTCTC Bsp1286I 13 GAGCAC G[AGT]GC[ACT]^C 147 GGGCAC BstYI 21 AGATCT [GA]^GATC[CT] 338 AGATCC EarI 394 CTCTTC CTCTTC(1/4) 10 GAAGAG HphI 72 GGTGA GGTGA(8/7) 177 GGTGA Ksp632I 394 CTCTTC CTCTTC(1/4) 10 GAAGAG MflI 21 AGATCT [GA]^GATC[CT] 338 AGATCC MseI 95 TTAA T^TAA 320 TTAA NlaIV 148 GGCACC GG[ACGT]^[ACGT]CC 309 GGTGCC SfcI 153 CTACAG C^T[GA][CT]AG 417 CTGCAG TaqI 26 TCGA T^CGA 335 TCGA Tru9I 95 TTAA T^TAA 320 TTAA TthHB8I 26 TCGA T^CGA 335 TCGA XhoII 21 AGATCT [GA]^GATC[CT] 338 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AciI AluI BbvI BsiYI BslI BsrI BssKI BstNI Cfr13I DpnII EcoRII Fnu4HI HaeIII ItaI MboI MboII MnlI MvaI MwoI NdeII Sau3AI Sau96I ScrFI SfaNI TseI TspRI