(Circular) (Six-base) MAPSORT of: pFN21AE2391.seq Check: 3992 from: 1 to: 6256 pFN21AE2391 6256 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2443 2443 Size: 6256 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4066 278 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2403 2525 3599 1937 Size: 466 122 1074 4594 Fragments arranged by size: 4594 1074 466 122 Acc65I G'GTAC_C Cuts at: 3577 3577 Size: 6256 AclI AA'CG_TT Cuts at: 1563 4383 4756 1563 Size: 2820 373 3063 Fragments arranged by size: 3063 2820 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2288 2301 2631 3329 4319 5703 5971 1632 Size: 656 13 330 698 990 1384 268 1917 Fragments arranged by size: 1917 1384 990 698 656 330 268 13 AfeI AGC'GCT Cuts at: 3548 3548 Size: 6256 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6059 AflIII A'CryG_T Cuts at: 1203 3084 5155 6132 1203 Size: 1881 2071 977 1327 Fragments arranged by size: 2071 1881 1327 977 AgeI A'CCGG_T Cuts at: 5087 5087 Size: 6256 AhdI GACnn_n'nnGTC Cuts at: 4985 4985 Size: 6256 AleI CACnn'nnGTG Cuts at: 1166 3026 1166 Size: 1860 4396 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2957 3044 1184 Size: 1773 87 4396 Fragments arranged by size: 4396 1773 87 AlwNI CAG_nnn'CTG Cuts at: 3999 5571 3999 Size: 1572 4684 ApaI G_GGCC'C Cuts at: 3124 3124 Size: 6256 ApaLI G'TGCA_C Cuts at: 4313 5469 4313 Size: 1156 5100 ApoI r'AATT_y Cuts at: 1493 2285 3565 3791 3943 6052 1493 Size: 792 1280 226 152 2109 1697 Fragments arranged by size: 2109 1697 1280 792 226 152 AseI AT'TA_AT Cuts at: 160 1032 4810 160 Size: 872 3778 1606 Fragments arranged by size: 3778 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 AvaI C'yCGr_G Cuts at: 1870 1943 3581 1870 Size: 73 1638 4545 Fragments arranged by size: 4545 1638 73 BamHI G'GATC_C Cuts at: 3586 3953 3586 Size: 367 5889 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2094 2767 3240 3577 5032 Size: 325 217 627 307 673 473 337 1455 Cuts at: 5032 618 Size: 1842 Fragments arranged by size: 1842 1455 673 627 473 337 325 307 217 BanII G_rGCy'C Cuts at: 1391 2130 2683 3078 3124 3575 1391 Size: 739 553 395 46 451 4072 Fragments arranged by size: 4072 739 553 451 395 46 BbeI G_GCGC'C Cuts at: 3244 3244 Size: 6256 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2797 2840 2866 928 Size: 971 898 43 26 4318 Fragments arranged by size: 4318 971 898 43 26 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4447 4481 1903 Size: 34 2510 34 3678 Fragments arranged by size: 3678 2510 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3143 4148 5358 3143 Size: 1005 1210 4041 Fragments arranged by size: 4041 1210 1005 BclI T'GATC_A Cuts at: 1548 1548 Size: 6256 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4867 136 Size: 107 122 71 631 605 3195 1525 Fragments arranged by size: 3195 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2376 6251 2376 Size: 3875 2381 BlpI GC'TnA_GC Cuts at: 3673 3673 Size: 6256 Bme1580I G_kGCm'C Cuts at: 1790 2772 3124 3458 4317 5473 1790 Size: 982 352 334 859 1156 2573 Fragments arranged by size: 2573 1156 982 859 352 334 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6256 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2036 3987 4945 Size: 416 53 83 399 24 619 1951 958 Cuts at: 4945 442 Size: 1753 Fragments arranged by size: 1951 1753 958 619 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6256 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2369 3080 4916 1239 Size: 1130 711 1836 2579 Fragments arranged by size: 2579 1836 1130 711 Bpu10I CC'TnA_GC Cuts at: 2546 2546 Size: 6256 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2489 2504 3167 4381 5246 5544 2489 Size: 15 663 1214 865 298 3201 Fragments arranged by size: 3201 1214 865 663 298 15 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2210 3056 4919 882 Size: 617 711 846 1863 2219 Fragments arranged by size: 2219 1863 846 711 617 BsaAI yAC'GTr Cuts at: 493 1204 4047 493 Size: 711 2843 2702 Fragments arranged by size: 2843 2702 711 BsaBI GATnn'nnATC Cuts at: 6250 6250 Size: 6256 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3241 3437 4063 4445 Size: 53 83 186 936 1708 196 626 382 Cuts at: 4445 5080 275 Size: 635 1451 Fragments arranged by size: 1708 1451 936 635 626 382 196 186 83 53 BsaWI w'CCGG_w Cuts at: 4689 5087 5361 5508 5919 4689 Size: 398 274 147 411 5026 Fragments arranged by size: 5026 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2180 2210 3509 3539 1178 Size: 30 165 30 777 30 1299 30 3895 Fragments arranged by size: 3895 1299 777 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 2093 2165 2228 2440 3148 3182 3389 Size: 904 3 72 63 212 708 34 207 Cuts at: 3389 1186 Size: 4053 Fragments arranged by size: 4053 904 708 212 207 72 63 34 3 BseYI C'CCAG_C Cuts at: 2061 2696 3017 3094 3110 3265 3285 3501 5459 Size: 635 321 77 16 155 20 216 1958 Cuts at: 5459 5955 2061 Size: 496 2362 Fragments arranged by size: 2362 1958 635 496 321 216 155 77 20 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3065 3237 3557 1163 Size: 573 1329 172 320 3862 Fragments arranged by size: 3862 1329 573 320 172 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2654 3706 4467 5495 1540 Size: 275 186 653 1052 761 1028 2301 Fragments arranged by size: 2301 1052 1028 761 653 275 186 BsiHKAI G_wGCw'C Cuts at: 2130 2247 2781 2859 2997 3078 4317 4402 5473 Size: 117 534 78 138 81 1239 85 1071 Cuts at: 5473 2130 Size: 2913 Fragments arranged by size: 2913 1239 1071 534 138 117 85 81 78 BsmI GAATG_Cn' Cuts at: 2281 3767 3860 2281 Size: 1486 93 4677 Fragments arranged by size: 4677 1486 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2130 2247 2683 2772 2781 2859 2997 Size: 399 340 117 436 89 9 78 138 Cuts at: 2997 3078 3124 3458 3575 4317 4402 5473 1391 Size: 81 46 334 117 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 436 399 340 334 138 117 117 89 85 81 78 46 9 BspEI T'CCGG_A Cuts at: 5919 5919 Size: 6256 BspHI T'CATG_A Cuts at: 4145 5875 4145 Size: 1730 4526 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2443 3611 844 Size: 1599 1168 3489 Fragments arranged by size: 3489 1599 1168 BsrBI CCG'CTC Cuts at: 1394 1823 2083 2448 3223 4143 1394 Size: 429 260 365 775 920 3507 Fragments arranged by size: 3507 920 775 429 365 260 BsrDI GCAATG_nn' Cuts at: 65 1248 4751 4925 65 Size: 1183 3503 174 1396 Fragments arranged by size: 3503 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2262 2649 2653 2815 3258 4900 5087 Size: 110 452 387 4 162 443 1642 187 Cuts at: 5087 1700 Size: 2869 Fragments arranged by size: 2869 1642 452 443 387 187 162 110 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6256 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6256 BssSI C'ACGA_G Cuts at: 4316 5328 4316 Size: 1012 5244 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6256 BstBI TT'CG_AA Cuts at: 5060 5060 Size: 6256 BstEII G'GTnAC_C Cuts at: 1184 2962 1184 Size: 1778 4478 BstXI CCAn_nnnn'nTGG Cuts at: 2914 2974 2914 Size: 60 6196 BstYI r'GATC_y Cuts at: 1972 2376 3465 3586 3953 4338 4355 5807 5893 Size: 404 1089 121 367 385 17 1452 86 Cuts at: 5893 5905 6251 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1089 404 385 367 346 121 86 17 12 BstZ17I GTA'TAC Cuts at: 2526 2526 Size: 6256 BtgI C'CryG_G Cuts at: 513 1065 2004 2136 2586 2913 2967 513 Size: 552 939 132 450 327 54 3802 Fragments arranged by size: 3802 939 552 450 327 132 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5517 Fragments arranged by size: 5517 683 56 BtsI GCAGTG_nn' Cuts at: 3199 3249 3772 4566 4586 3199 Size: 50 523 794 20 4869 Fragments arranged by size: 4869 794 523 50 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6256 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6221 DraI TTT'AAA Cuts at: 3560 3560 Size: 6256 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5263 809 Size: 507 3947 1802 Fragments arranged by size: 3947 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2529 2651 2655 Size: 54 1425 50 275 18 699 122 4 Cuts at: 2655 2844 2916 3234 3703 4592 6221 8 Size: 189 72 318 469 889 1629 43 Fragments arranged by size: 1629 1425 889 699 469 318 275 189 122 72 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2651 3703 1537 Size: 275 839 1052 4090 Fragments arranged by size: 4090 1052 839 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2750 2951 3059 3302 4186 1353 Size: 1397 201 108 243 884 3423 Fragments arranged by size: 3423 1397 884 243 201 108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2720 4843 5217 5363 387 Size: 1064 138 1131 2123 374 146 1280 Fragments arranged by size: 2123 1280 1131 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2128 3076 2128 Size: 948 5308 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2288 2301 2369 2631 3080 3329 4319 Size: 393 656 13 68 262 449 249 990 Cuts at: 4319 4916 5703 5971 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 990 787 656 597 449 393 268 262 249 68 13 EcoNI CCTnn'n_nnAGG Cuts at: 2555 2555 Size: 6256 EcoO109I rG'GnC_Cy Cuts at: 3120 3121 3517 3120 Size: 1 396 5859 Fragments arranged by size: 5859 396 1 EcoRI G'AATT_C Cuts at: 3565 3943 3565 Size: 378 5878 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2761 3070 2761 Size: 309 5947 FseI GG_CCGG'CC Cuts at: 2657 2657 Size: 6256 FspI TGC'GCA Cuts at: 4762 4762 Size: 6256 HaeII r_GCGC'y Cuts at: 1678 3244 3550 5403 1678 Size: 1566 306 1853 2531 Fragments arranged by size: 2531 1853 1566 306 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2822 2854 3200 3232 3509 Size: 32 135 32 1250 32 346 32 277 Cuts at: 3509 3541 4897 4929 4971 5003 1373 Size: 32 1356 32 42 32 2626 Fragments arranged by size: 2626 1356 1250 346 277 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2665 2797 3343 3600 3846 669 Size: 988 281 727 132 546 257 246 3079 Fragments arranged by size: 3079 988 727 546 281 257 246 132 HindIII A'AGCT_T Cuts at: 748 748 Size: 6256 HpaI GTT'AAC Cuts at: 3343 3846 3343 Size: 503 5753 KasI G'GCGC_C Cuts at: 3240 3240 Size: 6256 KpnI G_GTAC'C Cuts at: 3581 3581 Size: 6256 MfeI C'AATT_G Cuts at: 3855 3855 Size: 6256 MluI A'CGCG_T Cuts at: 6132 6132 Size: 6256 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2211 5370 5554 1305 Size: 906 3159 184 2007 Fragments arranged by size: 3159 2007 906 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2846 2918 3236 10 Size: 54 1425 343 1014 72 318 3030 Fragments arranged by size: 3030 1425 1014 343 318 72 54 MslI CAynn'nnrTG Cuts at: 518 1166 2765 2852 2912 2972 3026 3449 4214 Size: 648 1599 87 60 60 54 423 765 Cuts at: 4214 4573 4732 518 Size: 359 159 2042 Fragments arranged by size: 2042 1599 765 648 423 359 159 87 60 60 54 MspA1I CmG'CkG Cuts at: 1606 2061 3317 3387 3672 4349 5497 5742 1606 Size: 455 1256 70 285 677 1148 245 2120 Fragments arranged by size: 2120 1256 1148 677 455 285 245 70 NaeI GCC'GGC Cuts at: 2655 3260 2655 Size: 605 5651 NarI GG'CG_CC Cuts at: 3241 3241 Size: 6256 NcoI C'CATG_G Cuts at: 513 1065 2004 2913 2967 513 Size: 552 939 909 54 3802 Fragments arranged by size: 3802 939 909 552 54 NdeI CA'TA_TG Cuts at: 387 387 Size: 6256 NgoMIV G'CCGG_C Cuts at: 2653 3258 2653 Size: 605 5651 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6256 NotI GC'GGCC_GC Cuts at: 3703 3703 Size: 6256 NspI r_CATG'y Cuts at: 1132 1229 3088 3614 5159 1132 Size: 97 1859 526 1545 2229 Fragments arranged by size: 2229 1859 1545 526 97 PasI CC'CwG_GG Cuts at: 1348 3116 3145 1348 Size: 1768 29 4459 Fragments arranged by size: 4459 1768 29 PciI A'CATG_T Cuts at: 3084 5155 3084 Size: 2071 4185 PflMI CCAn_nnn'nTGG Cuts at: 1108 2235 1108 Size: 1127 5129 PfoI T'CCnGG_A Cuts at: 3057 3057 Size: 6256 PmeI GTTT'AAAC Cuts at: 3560 3560 Size: 6256 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4325 4357 5838 5870 4325 Size: 32 1481 32 4711 Fragments arranged by size: 4711 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3517 3517 Size: 6256 PshAI GACnn'nnGTC Cuts at: 1613 3440 1613 Size: 1827 4429 PsiI TTA'TAA Cuts at: 3826 3826 Size: 6256 PspOMI G'GGCC_C Cuts at: 3120 3120 Size: 6256 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2564 2596 2564 Size: 32 6224 PstI C_TGCA'G Cuts at: 830 3477 3608 830 Size: 2647 131 3478 Fragments arranged by size: 3478 2647 131 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6256 PvuII CAG'CTG Cuts at: 2061 3317 3387 2061 Size: 1256 70 4930 Fragments arranged by size: 4930 1256 70 SacI G_AGCT'C Cuts at: 2130 3078 2130 Size: 948 5308 SalI G'TCGA_C Cuts at: 1936 3598 1936 Size: 1662 4594 SanDI GG'GwC_CC Cuts at: 3517 3517 Size: 6256 SapI GCTCTTCn'nnn_ Cuts at: 2951 3059 2951 Size: 108 6148 SbfI CC_TGCA'GG Cuts at: 3477 3608 3477 Size: 131 6125 ScaI AGT'ACT Cuts at: 3173 4504 6216 3173 Size: 1331 1712 3213 Fragments arranged by size: 3213 1712 1331 SexAI A'CCwGG_T Cuts at: 2799 2799 Size: 6256 SfcI C'TryA_G Cuts at: 826 1045 3473 3604 4739 5420 5611 826 Size: 219 2428 131 1135 681 191 1471 Fragments arranged by size: 2428 1471 1135 681 219 191 131 SfoI GGC'GCC Cuts at: 3242 3242 Size: 6256 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6256 SmaI CCC'GGG Cuts at: 1872 3583 1872 Size: 1711 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2504 2519 3182 4360 5261 5523 Size: 197 926 561 15 663 1178 901 262 Cuts at: 5523 820 Size: 1553 Fragments arranged by size: 1553 1178 926 901 663 561 262 197 15 SnaBI TAC'GTA Cuts at: 493 4047 493 Size: 3554 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6256 SphI G_CATG'C Cuts at: 1132 3614 1132 Size: 2482 3774 SspI AAT'ATT Cuts at: 5 52 4180 5 Size: 47 4128 2081 Fragments arranged by size: 4128 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6256 StyI C'CwwG_G Cuts at: 513 1065 2004 2565 2913 2967 3159 3695 513 Size: 552 939 561 348 54 192 536 3074 Fragments arranged by size: 3074 939 561 552 536 348 192 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3031 3038 4283 4300 4453 4638 5083 Size: 1255 1451 7 1245 17 153 185 445 Cuts at: 5083 325 Size: 1498 Fragments arranged by size: 1498 1451 1255 1245 445 185 153 17 7 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2421 3171 4502 Size: 274 80 33 51 1443 444 750 1331 Cuts at: 4502 6214 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1331 750 444 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2312 2344 6216 6248 2312 Size: 32 3872 32 2320 Fragments arranged by size: 3872 2320 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6256 XbaI T'CTAG_A Cuts at: 3592 3592 Size: 6256 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2665 2683 3509 2665 Size: 18 826 5412 Fragments arranged by size: 5412 826 18 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6256 XmaI C'CCGG_G Cuts at: 1870 3581 1870 Size: 1711 4545 XmnI GAAnn'nnTTC Cuts at: 3053 4385 3053 Size: 1332 4924 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4064 276 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoRV FspAI NruI NsiI PacI PmlI PspXI RsrII SacII SfiI SrfI SwaI