Restriction map for pFN21AE2391
 (Linear) (Six-base) MAPSORT of: KIEE2391.seq  Check: 1662  from: 1  to: 1551



KIEE2391.orf

 With 182 enzymes: SgfI * 

                        January 26, 2012 13:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     438    1551 
   Size:        438    1113
 
AccI GT'mk_AC

Cuts at:      0     398     520    1551 
   Size:        398     122    1031

  Fragments arranged by size: 

                1031     398     122
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     296     626    1324    1551 
   Size:        283      13     330     698     227

  Fragments arranged by size: 

                 698     330     283     227      13
 
AfeI AGC'GCT

Cuts at:      0    1543    1551 
   Size:       1543       8
 
AflIII A'CryG_T

Cuts at:      0    1079    1551 
   Size:       1079     472
 
AleI CACnn'nnGTG

Cuts at:      0    1021    1551 
   Size:       1021     530
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     952    1039    1551 
   Size:        952      87     512

  Fragments arranged by size: 

                 952     512      87
 
ApaI G_GGCC'C

Cuts at:      0    1119    1551 
   Size:       1119     432
 
ApoI r'AATT_y

Cuts at:      0     280    1551 
   Size:        280    1271
 
BanI G'GyrC_C

Cuts at:      0      89     762    1235    1551 
   Size:         89     673     473     316

  Fragments arranged by size: 

                 673     473     316      89
 
BanII G_rGCy'C

Cuts at:      0     125     678    1073    1119    1551 
   Size:        125     553     395      46     432

  Fragments arranged by size: 

                 553     432     395     125      46
 
BbeI G_GCGC'C

Cuts at:      0    1239    1551 
   Size:       1239     312
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     792     835     861    1551 
   Size:        792      43      26     690

  Fragments arranged by size: 

                 792     690      43      26
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1138    1551 
   Size:       1138     413
 
BglII A'GATC_T

Cuts at:      0     371    1551 
   Size:        371    1180
 
Bme1580I G_kGCm'C

Cuts at:      0     767    1119    1453    1551 
   Size:        767     352     334      98

  Fragments arranged by size: 

                 767     352     334      98
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      31    1551 
   Size:         31    1520
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364    1075    1551 
   Size:        364     711     476

  Fragments arranged by size: 

                 711     476     364
 
Bpu10I CC'TnA_GC

Cuts at:      0     541    1551 
   Size:        541    1010
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     484     499    1162    1551 
   Size:        484      15     663     389

  Fragments arranged by size: 

                 663     484     389      15
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     205    1051    1551 
   Size:        205     846     500

  Fragments arranged by size: 

                 846     500     205
 
BsaHI Gr'CG_yC

Cuts at:      0    1236    1432    1551 
   Size:       1236     196     119

  Fragments arranged by size: 

                1236     196     119
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     175     205    1504    1534    1551 
   Size:        175      30    1299      30      17

  Fragments arranged by size: 

                1299     175      30      30      17
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      85      88     160     223     435    1143    1177    1384 
   Size:         85       3      72      63     212     708      34     207

Cuts at:   1384    1551 
   Size:        167

  Fragments arranged by size: 

                 708     212     207     167      85      72      63      34
                   3
 
BseYI C'CCAG_C

Cuts at:      0      56     691    1012    1089    1105    1260    1280    1496 
   Size:         56     635     321      77      16     155      20     216

Cuts at:   1496    1551 
   Size:         55

  Fragments arranged by size: 

                 635     321     216     155      77      56      55      20
                  16
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1060    1232    1551 
   Size:       1060     172     319

  Fragments arranged by size: 

                1060     319     172
 
BsiEI CG_ry'CG

Cuts at:      0     649    1551 
   Size:        649     902
 
BsiHKAI G_wGCw'C

Cuts at:      0     125     242     776     854     992    1073    1551 
   Size:        125     117     534      78     138      81     478

  Fragments arranged by size: 

                 534     478     138     125     117      81      78
 
BsmI GAATG_Cn'

Cuts at:      0     276    1551 
   Size:        276    1275
 
Bsp1286I G_dGCh'C

Cuts at:      0     125     242     678     767     776     854     992    1073 
   Size:        125     117     436      89       9      78     138      81

Cuts at:   1073    1119    1453    1551 
   Size:         46     334      98

  Fragments arranged by size: 

                 436     334     138     125     117      98      89      81
                  78      46       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     438    1551 
   Size:        438    1113
 
BsrBI CCG'CTC

Cuts at:      0      78     443    1218    1551 
   Size:         78     365     775     333

  Fragments arranged by size: 

                 775     365     333      78
 
BsrFI r'CCGG_y

Cuts at:      0     257     644     648     810    1253    1551 
   Size:        257     387       4     162     443     298

  Fragments arranged by size: 

                 443     387     298     257     162       4
 
BstEII G'GTnAC_C

Cuts at:      0     957    1551 
   Size:        957     594
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     909     969    1551 
   Size:        909      60     582

  Fragments arranged by size: 

                 909     582      60
 
BstYI r'GATC_y

Cuts at:      0     371    1460    1551 
   Size:        371    1089      91

  Fragments arranged by size: 

                1089     371      91
 
BstZ17I GTA'TAC

Cuts at:      0     521    1551 
   Size:        521    1030
 
BtgI C'CryG_G

Cuts at:      0     131     581     908     962    1551 
   Size:        131     450     327      54     589

  Fragments arranged by size: 

                 589     450     327     131      54
 
BtsI GCAGTG_nn'

Cuts at:      0    1194    1244    1551 
   Size:       1194      50     307

  Fragments arranged by size: 

                1194     307      50
 
EaeI y'GGCC_r

Cuts at:      0     524     646     650     839     911    1229    1551 
   Size:        524     122       4     189      72     318     322

  Fragments arranged by size: 

                 524     322     318     189     122      72       4
 
EagI C'GGCC_G

Cuts at:      0     646    1551 
   Size:        646     905
 
EarI CTCTTCn'nnn_

Cuts at:      0     745     946    1054    1297    1551 
   Size:        745     201     108     243     254

  Fragments arranged by size: 

                 745     254     243     201     108
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     715    1551 
   Size:        715     836
 
EcoICRI GAG'CTC

Cuts at:      0     123    1071    1551 
   Size:        123     948     480

  Fragments arranged by size: 

                 948     480     123
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     296     364     626    1075    1324    1551 
   Size:        283      13      68     262     449     249     227

  Fragments arranged by size: 

                 449     283     262     249     227      68      13
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     550    1551 
   Size:        550    1001
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1115    1116    1512    1551 
   Size:       1115       1     396      39

  Fragments arranged by size: 

                1115     396      39       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     756    1065    1551 
   Size:        756     309     486

  Fragments arranged by size: 

                 756     486     309
 
FseI GG_CCGG'CC

Cuts at:      0     652    1551 
   Size:        652     899
 
HaeII r_GCGC'y

Cuts at:      0    1239    1545    1551 
   Size:       1239     306       6

  Fragments arranged by size: 

                1239     306       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     817     849    1195    1227    1504    1536    1551 
   Size:        817      32     346      32     277      32      15

  Fragments arranged by size: 

                 817     346     277      32      32      32      15
 
HincII GTy'rAC

Cuts at:      0     660     792    1338    1551 
   Size:        660     132     546     213

  Fragments arranged by size: 

                 660     546     213     132
 
HpaI GTT'AAC

Cuts at:      0    1338    1551 
   Size:       1338     213
 
KasI G'GCGC_C

Cuts at:      0    1235    1551 
   Size:       1235     316
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     206    1551 
   Size:        206    1345
 
MscI TGG'CCA

Cuts at:      0     841     913    1231    1551 
   Size:        841      72     318     320

  Fragments arranged by size: 

                 841     320     318      72
 
MslI CAynn'nnrTG

Cuts at:      0     760     847     907     967    1021    1444    1551 
   Size:        760      87      60      60      54     423     107

  Fragments arranged by size: 

                 760     423     107      87      60      60      54
 
MspA1I CmG'CkG

Cuts at:      0      56    1312    1382    1551 
   Size:         56    1256      70     169

  Fragments arranged by size: 

                1256     169      70      56
 
NaeI GCC'GGC

Cuts at:      0     650    1255    1551 
   Size:        650     605     296

  Fragments arranged by size: 

                 650     605     296
 
NarI GG'CG_CC

Cuts at:      0    1236    1551 
   Size:       1236     315
 
NcoI C'CATG_G

Cuts at:      0     908     962    1551 
   Size:        908      54     589

  Fragments arranged by size: 

                 908     589      54
 
NgoMIV G'CCGG_C

Cuts at:      0     648    1253    1551 
   Size:        648     605     298

  Fragments arranged by size: 

                 648     605     298
 
NspI r_CATG'y

Cuts at:      0    1083    1551 
   Size:       1083     468
 
PasI CC'CwG_GG

Cuts at:      0    1111    1140    1551 
   Size:       1111      29     411

  Fragments arranged by size: 

                1111     411      29
 
PciI A'CATG_T

Cuts at:      0    1079    1551 
   Size:       1079     472
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     230    1551 
   Size:        230    1321
 
PfoI T'CCnGG_A

Cuts at:      0    1052    1551 
   Size:       1052     499
 
PpuMI rG'GwC_Cy

Cuts at:      0    1512    1551 
   Size:       1512      39
 
PshAI GACnn'nnGTC

Cuts at:      0    1435    1551 
   Size:       1435     116
 
PspOMI G'GGCC_C

Cuts at:      0    1115    1551 
   Size:       1115     436
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     559     591    1551 
   Size:        559      32     960

  Fragments arranged by size: 

                 960     559      32
 
PstI C_TGCA'G

Cuts at:      0    1472    1551 
   Size:       1472      79
 
PvuII CAG'CTG

Cuts at:      0      56    1312    1382    1551 
   Size:         56    1256      70     169

  Fragments arranged by size: 

                1256     169      70      56
 
SacI G_AGCT'C

Cuts at:      0     125    1073    1551 
   Size:        125     948     478

  Fragments arranged by size: 

                 948     478     125
 
SanDI GG'GwC_CC

Cuts at:      0    1512    1551 
   Size:       1512      39
 
SapI GCTCTTCn'nnn_

Cuts at:      0     946    1054    1551 
   Size:        946     108     497

  Fragments arranged by size: 

                 946     497     108
 
SbfI CC_TGCA'GG

Cuts at:      0    1472    1551 
   Size:       1472      79
 
ScaI AGT'ACT

Cuts at:      0    1168    1551 
   Size:       1168     383
 
SexAI A'CCwGG_T

Cuts at:      0     794    1551 
   Size:        794     757
 
SfcI C'TryA_G

Cuts at:      0    1468    1551 
   Size:       1468      83
 
SfoI GGC'GCC

Cuts at:      0    1237    1551 
   Size:       1237     314
 
SmlI C'TyrA_G

Cuts at:      0     499     514    1177    1551 
   Size:        499      15     663     374

  Fragments arranged by size: 

                 663     499     374      15
 
StyI C'CwwG_G

Cuts at:      0     560     908     962    1154    1551 
   Size:        560     348      54     192     397

  Fragments arranged by size: 

                 560     397     348     192      54
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1026    1033    1551 
   Size:       1026       7     518

  Fragments arranged by size: 

                1026     518       7
 
TatI w'GTAC_w

Cuts at:      0     416    1166    1551 
   Size:        416     750     385

  Fragments arranged by size: 

                 750     416     385
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     307     339    1551 
   Size:        307      32    1212

  Fragments arranged by size: 

                1212     307      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     660     678    1504    1551 
   Size:        660      18     826      47

  Fragments arranged by size: 

                 826     660      47      18
 
XmnI GAAnn'nnTTC

Cuts at:      0    1048    1551 
   Size:       1048     503
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AleI     AlfI     ApaI
     ApoI     BanI    BanII     BbeI     BbsI    BciVI    BglII Bme1580I
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI
    BsrFI   BstEII    BstXI    BstYI  BstZ17I     BtgI     BtsI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
     FseI    HaeII    Hin4I   HincII     HpaI     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI
     PciI    PflMI     PfoI    PpuMI    PshAI   PspOMI     PsrI     PstI
    PvuII     SacI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfoI     SmlI     StyI    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AloI    AlwNI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BbvCI     BcgI     BclI    BfrBI     BglI     BlpI    BmgBI     BmtI
     BplI    BsaAI    BsaBI    BsaWI    BsiWI    BsmBI    BspEI    BspHI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI    EcoRI    EcoRV     FspI
    FspAI  HindIII     KpnI     MfeI     MluI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PmeI     PmlI     PpiI     PsiI    PspXI
     PvuI    RsrII    SacII     SalI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI  Tth111I
     XbaI     XhoI     XmaI     ZraI
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