################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AccII AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MamI MluI MluNI MscI MspA1I MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 1250 TCCGGA T^CCGGA AclI 635 AACGTT AA^CGTT AcsI 1295 GAATTC [GA]^AATT[CT] AfaI 1323 GTAC GT^AC AlwI 453 GGATC GGATC(4/5) AocI 1115 CCTCAGG CC^T[ACGT]AGG ApoI 1295 GAATTC [GA]^AATT[CT] AspI 786 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 972 GGTCC G^G[AT]CC AvrII 975 CCTAGG C^CTAGG BbsI 1362 GTCTTC GAAGAC(2/6) BcnI 1197 CCGGG CC^[GC]GG BglII 1241 AGATCT A^GATCT BlnI 975 CCTAGG C^CTAGG BpuAI 1362 GTCTTC GAAGAC(2/6) BseAI 1250 TCCGGA T^CCGGA BseRI 1393 CTCCTC GAGGAG(10/8) BsmAI 586 GAGAC GTCTC(1/5) BsmI 514 GCATTC GAATGC(1/-1) BspEI 1250 TCCGGA T^CCGGA BspMI 1186 ACCTGC ACCTGC(4/8) BstAPI 48 GCACATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1298 TTCGAA TT^CGAA BstXI 443 CCATTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1241 AGATCT [GA]^GATC[CT] Bsu36I 1115 CCTCAGG CC^T[ACGT]AGG DraII 971 AGGTCCT [GA]G^G[ACGT]CC[CT] EaeI 1173 TGGCCG [CT]^GGCC[GA] Eco81I 1115 CCTCAGG CC^T[ACGT]AGG EcoNI 571 CCTATTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 971 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 1295 GAATTC G^AATTC HincII 783 GTTGAC GT[CT]^[GA]AC HindII 783 GTTGAC GT[CT]^[GA]AC MflI 1241 AGATCT [GA]^GATC[CT] MroI 1250 TCCGGA T^CCGGA MwoI 48 GCACATTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 1197 CCGGG CC^[GC]GG NspV 1298 TTCGAA TT^CGAA PflFI 786 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1329 GAGTC GAGTC(4/5) PpuMI 971 AGGTCCT [GA]G^G[AT]CC[CT] Psp1406I 635 AACGTT AA^CGTT PstI 253 CTGCAG CTGCA^G RsaI 1323 GTAC GT^AC SfuI 1298 TTCGAA TT^CGAA Tth111I 786 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 1241 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 792 GTCTAC GT^[AC][GT]AC 1135 GTCTAC AciI 419 CCGC CCGC(-3/-1) 289 GCGG AluI 171 AGCT AG^CT 251 AGCT AspHI 46 GAGCAC G[AT]GC[AT]^C 60 GAGCAC BfaI 976 CTAG C^TAG 1381 CTAG BmyI 46 GAGCAC G[AGT]GC[ACT]^C 60 GAGCAC BsaJI 284 CCTTGG C^C[ACGT][ACGT]GG 975 CCTAGG BsaWI 831 ACCGGA [AT]^CCGG[AT] 1250 TCCGGA BsgI 160 CTGCAC GTGCAG(16/14) 937 GTGCAG BsiHKAI 46 GAGCAC G[AT]GC[AT]^C 60 GAGCAC BsmFI 568 GTCCC GGGAC(10/14) 1013 GTCCC Bsp1286I 46 GAGCAC G[AGT]GC[ACT]^C 60 GAGCAC BsrDI 555 CATTGC GCAATG(2/0) 844 GCAATG DpnII 454 GATC ^GATC 1242 GATC EcoT14I 284 CCTTGG C^C[AT][AT]GG 975 CCTAGG EcoT22I 430 ATGCAT ATGCA^T 1166 ATGCAT MaeI 976 CTAG C^TAG 1381 CTAG MboI 454 GATC ^GATC 1242 GATC NdeII 454 GATC ^GATC 1242 GATC NlaIV 362 GGCCCC GG[ACGT]^[ACGT]CC 1340 GGCTCC NsiI 430 ATGCAT ATGCA^T 1166 ATGCAT SapI 1423 GAAGAGC GCTCTTC(1/4) 172 GCTCTTC Sau3AI 454 GATC ^GATC 1242 GATC StyI 284 CCTTGG C^C[AT][AT]GG 975 CCTAGG TaqI 861 TCGA T^CGA 1299 TCGA TthHB8I 861 TCGA T^CGA 1299 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### BbvI BpmI BsiYI BslI BsrI BssKI BstF5I BstNI Cfr13I DdeI EarI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboII MfeI MnlI MseI MslI MspI MunI MvaI NlaIII Sau96I ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp45I Tsp509I TspRI