################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## Acc65I AccII AclI AgeI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp718I AspHI AspI AviII BbeI BbrPI BbvCI BglII BlpI Bpu1102I BsaBI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BsrBI BsrFI BsrGI BssHII BssSI BstEII BstPI BstUI BstXI CelII Cfr10I ClaI CpoI DraI DraIII DrdI Eco47III EcoO65I FauI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MamI MluI MvnI NaeI NarI NgoMIV NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 481 GACGTC GACGT^C AccIII 161 TCCGGA T^CCGGA AciI 14 CCGC CCGC(-3/-1) AflIII 1809 ACATGT A^C[GA][CT]GT AhdI 476 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 2005 ATTAAT AT^TAAT AsnI 2005 ATTAAT AT^TAAT AspEI 476 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 2645 CCCGAG C^[CT]CG[GA]G AvrII 1545 CCTAGG C^CTAGG BalI 2174 TGGCCA TGG^CCA BamHI 838 GGATCC G^GATCC BanI 814 GGCACC G^G[CT][GA]CC BanII 1563 GGGCTC G[GA]GC[CT]^C BcgI 1406 GCAATGATATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 862 CCGGG CC^[GC]GG BglI 181 GCCTTAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1545 CCTAGG C^CTAGG BsaAI 2044 TACGTG [CT]AC^GT[GA] BsaWI 161 TCCGGA [AT]^CCGG[AT] BseAI 161 TCCGGA T^CCGGA BsgI 1463 CTGCAC GTGCAG(16/14) BsiEI 11 CGGCCG CG[GA][CT]^CG BsmI 81 GAATGC GAATGC(1/-1) BsoBI 2645 CCCGAG C^[CT]CG[GA]G BspEI 161 TCCGGA T^CCGGA BspLU11I 1809 ACATGT A^CATGT BspMI 2215 GCAGGT ACCTGC(4/8) Bst1107I 1749 GTATAC GTA^TAC BstAPI 2434 GCATATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 838 GGATCC [GA]^GATC[CT] BstZ17I 1749 GTATAC GTA^TAC EagI 11 CGGCCG C^GGCCG Eam1105I 476 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 11 CGGCCG C^GGCCG Eco52I 11 CGGCCG C^GGCCG EcoRI 1083 GAATTC G^AATTC EcoT22I 309 ATGCAT ATGCA^T HaeII 2185 GGCGCT [GA]GCGC^[CT] MfeI 374 CAATTG C^AATTG MflI 838 GGATCC [GA]^GATC[CT] MluNI 2174 TGGCCA TGG^CCA MroI 161 TCCGGA T^CCGGA MscI 2174 TGGCCA TGG^CCA MunI 374 CAATTG C^AATTG NciI 862 CCGGG CC^[GC]GG NdeI 2266 CATATG CA^TATG NheI 766 GCTAGC G^CTAGC NsiI 309 ATGCAT ATGCA^T PshBI 2005 ATTAAT AT^TAAT PstI 1015 CTGCAG CTGCA^G ScaI 588 AGTACT AGT^ACT XbaI 423 TCTAGA T^CTAGA XcmI 326 CCAAACAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 838 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 124 GTAGAC GT^[AC][GT]AC 1749 GTATAC AocI 676 CCTCAGG CC^T[ACGT]AGG 952 CCTAAGG Asp700I 20 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2118 GAATCAGTTC BciVI 1287 GGATAC GTATCC(6/5) 2745 GGATAC BsaI 2686 GAGACC GGTCTC(1/5) 1324 GGTCTC BstBI 803 TTCGAA TT^CGAA 1733 TTCGAA Bsu36I 676 CCTCAGG CC^T[ACGT]AGG 952 CCTAAGG CfoI 1944 GCGC GCG^C 2186 GCGC DsaI 1454 CCATGG C^C[GA][CT]GG 1760 CCATGG EaeI 11 CGGCCG [CT]^GGCC[GA] 2174 TGGCCA Eco81I 676 CCTCAGG CC^T[ACGT]AGG 952 CCTAAGG EcoRV 691 GATATC GAT^ATC 1411 GATATC HgaI 1799 GACGC GACGC(5/10) 2285 GCGTC HhaI 1944 GCGC GCG^C 2186 GCGC HinP1I 1944 GCGC G^CGC 2186 GCGC MslI 1357 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2161 CATGATGATG NcoI 1454 CCATGG C^CATGG 1760 CCATGG NspI 551 GCATGT [GA]CATG^[CT] 1809 ACATGT NspV 803 TTCGAA TT^CGAA 1733 TTCGAA PvuII 1532 CAGCTG CAG^CTG 2131 CAGCTG SfuI 803 TTCGAA TT^CGAA 1733 TTCGAA XmnI 20 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2118 GAATCAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AcsI AcyI AfaI AflII AluI AlwI ApoI AvaII BbsI BbvI BclI BfaI BfrI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BsrDI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EarI EcoNI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII Hin1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PflMI PleI PpuMI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I