################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsaWI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MslI MunI NaeI NarI NgoMIV NheI NotI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AcyI 527 GACGCC G[GA]^CG[CT]C ApaI 360 GGGCCC GGGCC^C AspHI 343 GAGCTC G[AT]GC[AT]^C BalI 770 TGGCCA TGG^CCA BanI 89 GGCACC G^G[CT][GA]CC BbsI 304 GTCTTC GAAGAC(2/6) BfaI 22 CTAG C^TAG BlpI 651 GCTGAGC GC^T[ACGT]AGC BpmI 82 CTCCAG CTGGAG(16/14) Bpu1102I 651 GCTGAGC GC^T[ACGT]AGC BpuAI 304 GTCTTC GAAGAC(2/6) BsaHI 527 GACGCC G[GA]^CG[CT]C BsaI 329 GAGACC GGTCTC(1/5) BsiEI 195 CGACCG CG[GA][CT]^CG BsiHKAI 343 GAGCTC G[AT]GC[AT]^C BsmAI 329 GAGAC GTCTC(1/5) BsrBI 568 GAGCGG CCGCTC(-3/-3) CelII 651 GCTGAGC GC^T[ACGT]AGC CfoI 72 GCGC GCG^C DsaI 773 CCATGG C^C[GA][CT]GG EarI 420 CTCTTC CTCTTC(1/4) EcoNI 744 CCTCTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 313 GAATTC G^AATTC HhaI 72 GCGC GCG^C Hin1I 527 GACGCC G[GA]^CG[CT]C HinP1I 72 GCGC G^CGC HinfI 548 GAATC G^A[ACGT]TC Ksp632I 420 CTCTTC CTCTTC(1/4) MaeI 22 CTAG C^TAG MaeII 26 ACGT A^CGT MluNI 770 TGGCCA TGG^CCA MscI 770 TGGCCA TGG^CCA MseI 137 TTAA T^TAA NcoI 773 CCATGG C^CATGG NdeI 155 CATATG CA^TATG NruI 660 TCGCGA TCG^CGA PstI 763 CTGCAG CTGCA^G SacI 343 GAGCTC GAGCT^C StuI 950 AGGCCT AGG^CCT TfiI 548 GAATC G^A[AT]TC Tru9I 137 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 32 CGCG CG^CG 661 CGCG AcsI 133 AAATTT [GA]^AATT[CT] 313 GAATTC AlwI 18 GATCC GGATC(4/5) 877 GATCC AlwNI 227 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG 248 CAGAAGCTG ApoI 133 AAATTT [GA]^AATT[CT] 313 GAATTC AvaI 242 CTCGGG C^[CT]CG[GA]G 295 CCCGAG BanII 343 GAGCTC G[GA]GC[CT]^C 360 GGGCCC BsoBI 242 CTCGGG C^[CT]CG[GA]G 295 CCCGAG BspMI 761 ACCTGC ACCTGC(4/8) 820 GCAGGT BstUI 32 CGCG CG^CG 661 CGCG BstYI 17 AGATCC [GA]^GATC[CT] 876 AGATCC DpnII 18 GATC ^GATC 877 GATC HgaI 527 GACGC GACGC(5/10) 40 GCGTC MaeIII 607 GTCAC ^GT[ACGT]AC 679 GTCAC MboI 18 GATC ^GATC 877 GATC MflI 17 AGATCC [GA]^GATC[CT] 876 AGATCC MvnI 32 CGCG CG^CG 661 CGCG MwoI 840 GCACCGACGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 849 GCAGGGGCGGC NdeII 18 GATC ^GATC 877 GATC PpuMI 758 AGGACCT [GA]G^G[AT]CC[CT] 892 GGGACCC Sau3AI 18 GATC ^GATC 877 GATC SfaNI 4 GCATC GCATC(5/9) 533 GCATC SfcI 763 CTGCAG C^T[GA][CT]AG 780 CTACAG Tsp45I 607 GTCAC ^GT[GC]AC 679 GTCAC Tsp509I 134 AATT ^AATT 314 AATT XhoII 17 AGATCC [GA]^GATC[CT] 876 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AluI AvaII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MboII MnlI MspA1I MspI MvaI NciI NlaIII NlaIV PvuII Sau96I ScrFI StyI TseI TspRI