(Circular) (Six-base) MAPSORT of: pFN21AB8114.seq Check: 4074 from: 1 to: 5677 pFN21AB8114 5677 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2816 2816 Size: 5677 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3487 278 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3020 1937 Size: 1083 4594 Acc65I G'GTAC_C Cuts at: 2998 2998 Size: 5677 AclI AA'CG_TT Cuts at: 1563 3804 4177 1563 Size: 2241 373 3063 Fragments arranged by size: 3063 2241 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2366 2458 2624 3740 5124 5392 1632 Size: 734 92 166 1116 1384 268 1917 Fragments arranged by size: 1917 1384 1116 734 268 166 92 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5480 AflIII A'CryG_T Cuts at: 1203 4576 5553 1203 Size: 3373 977 1327 Fragments arranged by size: 3373 1327 977 AgeI A'CCGG_T Cuts at: 4508 4508 Size: 5677 AhdI GACnn_n'nnGTC Cuts at: 4406 4406 Size: 5677 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5677 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2739 1184 Size: 1555 4122 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2378 2410 2378 Size: 32 5645 AlwNI CAG_nnn'CTG Cuts at: 2237 2258 3420 4992 2237 Size: 21 1162 1572 2922 Fragments arranged by size: 2922 1572 1162 21 ApaI G_GGCC'C Cuts at: 2369 2369 Size: 5677 ApaLI G'TGCA_C Cuts at: 3734 4890 3734 Size: 1156 4521 ApoI r'AATT_y Cuts at: 1493 2138 2318 2986 3212 3364 5473 1493 Size: 645 180 668 226 152 2109 1697 Fragments arranged by size: 2109 1697 668 645 226 180 152 AseI AT'TA_AT Cuts at: 160 1032 4231 160 Size: 872 3199 1606 Fragments arranged by size: 3199 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 AvaI C'yCGr_G Cuts at: 1870 1943 2247 2300 3002 1870 Size: 73 304 53 702 4545 Fragments arranged by size: 4545 702 304 73 53 BamHI G'GATC_C Cuts at: 3007 3374 3007 Size: 367 5310 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2094 2998 4453 618 Size: 325 217 627 307 904 1455 1842 Fragments arranged by size: 1842 1455 904 627 325 307 217 BanII G_rGCy'C Cuts at: 1391 2352 2369 2996 1391 Size: 961 17 627 4072 Fragments arranged by size: 4072 961 627 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2302 928 Size: 971 403 4303 Fragments arranged by size: 4303 971 403 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3868 3902 1903 Size: 34 1931 34 3678 Fragments arranged by size: 3678 1931 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3569 4779 3569 Size: 1210 4467 BclI T'GATC_A Cuts at: 1548 1548 Size: 5677 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4288 136 Size: 107 122 71 631 605 2616 1525 Fragments arranged by size: 2616 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5672 5672 Size: 5677 BlpI GC'TnA_GC Cuts at: 2657 3094 2657 Size: 437 5240 Bme1580I G_kGCm'C Cuts at: 1790 2369 2445 2705 3738 4894 1790 Size: 579 76 260 1033 1156 2573 Fragments arranged by size: 2573 1156 1033 579 260 76 BmgBI CAC'GTC Cuts at: 1318 2032 1318 Size: 714 4963 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2654 3408 4366 Size: 416 53 83 399 24 1237 754 958 Cuts at: 4366 442 Size: 1753 Fragments arranged by size: 1753 1237 958 754 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5677 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2974 2974 Size: 5677 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2072 4337 1239 Size: 833 2265 2579 Fragments arranged by size: 2579 2265 833 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3802 4667 4965 3802 Size: 865 298 4514 Fragments arranged by size: 4514 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2328 4340 882 Size: 617 829 2012 2219 Fragments arranged by size: 2219 2012 829 617 BsaAI yAC'GTr Cuts at: 493 1204 3468 493 Size: 711 2264 2702 Fragments arranged by size: 2702 2264 711 BsaBI GATnn'nnATC Cuts at: 5671 5671 Size: 5677 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2533 3484 3866 4501 Size: 53 83 186 936 1000 951 382 635 Cuts at: 4501 275 Size: 1451 Fragments arranged by size: 1451 1000 951 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4110 4508 4782 4929 5340 4110 Size: 398 274 147 411 4447 Fragments arranged by size: 4447 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5452 Fragments arranged by size: 5452 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2318 2446 2449 2603 2963 1186 Size: 1132 128 3 154 360 3900 Fragments arranged by size: 3900 1132 360 154 128 3 BseYI C'CCAG_C Cuts at: 2810 2871 4880 5376 2810 Size: 61 2009 496 3111 Fragments arranged by size: 3111 2009 496 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5104 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2203 3127 3888 4916 1540 Size: 275 186 202 924 761 1028 2301 Fragments arranged by size: 2301 1028 924 761 275 202 186 BsiHKAI G_wGCw'C Cuts at: 2352 3738 3823 4894 2352 Size: 1386 85 1071 3135 Fragments arranged by size: 3135 1386 1071 85 BsmI GAATG_Cn' Cuts at: 3188 3281 3188 Size: 93 5584 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2352 2369 2445 2705 2996 3738 3823 Size: 399 562 17 76 260 291 742 85 Cuts at: 3823 4894 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 562 399 291 260 85 76 17 BspEI T'CCGG_A Cuts at: 5340 5340 Size: 5677 BspHI T'CATG_A Cuts at: 3566 5296 3566 Size: 1730 3947 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2775 2816 3032 844 Size: 1931 41 216 3489 Fragments arranged by size: 3489 1931 216 41 BsrBI CCG'CTC Cuts at: 1394 1823 2575 3564 1394 Size: 429 752 989 3507 Fragments arranged by size: 3507 989 752 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4172 4346 65 Size: 1183 2924 174 1396 Fragments arranged by size: 2924 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4321 4508 1700 Size: 110 2511 187 2869 Fragments arranged by size: 2869 2511 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5677 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5677 BssSI C'ACGA_G Cuts at: 3737 4749 3737 Size: 1012 4665 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5677 BstBI TT'CG_AA Cuts at: 4481 4481 Size: 5677 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5677 BstYI r'GATC_y Cuts at: 1972 2022 2881 3007 3374 3759 3776 5228 5314 Size: 50 859 126 367 385 17 1452 86 Cuts at: 5314 5326 5672 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 859 385 367 346 126 86 50 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2778 513 Size: 552 939 774 3412 Fragments arranged by size: 3412 939 774 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4938 Fragments arranged by size: 4938 683 56 BtsI GCAGTG_nn' Cuts at: 2269 3193 3987 4007 2269 Size: 924 794 20 3939 Fragments arranged by size: 3939 924 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5677 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5642 DraI TTT'AAA Cuts at: 2981 2981 Size: 5677 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4684 809 Size: 507 3368 1802 Fragments arranged by size: 3368 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2151 2209 2775 Size: 54 1425 50 275 18 321 58 566 Cuts at: 2775 3124 4013 5642 8 Size: 349 889 1629 43 Fragments arranged by size: 1629 1425 889 566 349 321 275 58 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3124 1537 Size: 275 1312 4090 Fragments arranged by size: 4090 1312 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2431 3607 1353 Size: 1078 1176 3423 Fragments arranged by size: 3423 1176 1078 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4264 4638 4784 387 Size: 1064 138 2675 374 146 1280 Fragments arranged by size: 2675 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2350 2350 Size: 5677 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2366 2458 2624 3740 4337 5124 Size: 393 440 294 92 166 1116 597 787 Cuts at: 5124 5392 1239 Size: 268 1524 Fragments arranged by size: 1524 1116 787 597 440 393 294 268 166 92 EcoNI CCTnn'n_nnAGG Cuts at: 2753 2753 Size: 5677 EcoO109I rG'GnC_Cy Cuts at: 2366 2408 2764 2898 2366 Size: 42 356 134 5145 Fragments arranged by size: 5145 356 134 42 EcoRI G'AATT_C Cuts at: 2318 2986 3364 2318 Size: 668 378 4631 Fragments arranged by size: 4631 668 378 FspI TGC'GCA Cuts at: 4183 4183 Size: 5677 HaeII r_GCGC'y Cuts at: 1678 4824 1678 Size: 3146 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2441 2473 2523 2555 4318 Size: 32 135 32 869 32 50 32 1763 Cuts at: 4318 4350 4392 4424 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1763 869 135 50 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3021 3267 669 Size: 988 281 1083 246 3079 Fragments arranged by size: 3079 1083 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5677 HpaI GTT'AAC Cuts at: 3267 3267 Size: 5677 KpnI G_GTAC'C Cuts at: 3002 3002 Size: 5677 MfeI C'AATT_G Cuts at: 3276 3276 Size: 5677 MluI A'CGCG_T Cuts at: 5553 5553 Size: 5677 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4791 4975 1305 Size: 3486 184 2007 Fragments arranged by size: 3486 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2777 10 Size: 54 1425 343 945 2910 Fragments arranged by size: 2910 1425 945 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3635 3994 4153 518 Size: 648 2469 359 159 2042 Fragments arranged by size: 2469 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2051 2602 2773 2810 3093 3770 4918 5163 Size: 445 551 171 37 283 677 1148 245 Cuts at: 5163 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 551 445 283 245 171 37 NcoI C'CATG_G Cuts at: 513 1065 2004 2778 513 Size: 552 939 774 3412 Fragments arranged by size: 3412 939 774 552 NdeI CA'TA_TG Cuts at: 387 2161 387 Size: 1774 3903 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5677 NotI GC'GGCC_GC Cuts at: 3124 3124 Size: 5677 NruI TCG'CGA Cuts at: 2667 2667 Size: 5677 NspI r_CATG'y Cuts at: 1132 1229 3035 4580 1132 Size: 97 1806 1545 2229 Fragments arranged by size: 2229 1806 1545 97 PasI CC'CwG_GG Cuts at: 1348 2438 1348 Size: 1090 4587 PciI A'CATG_T Cuts at: 4576 4576 Size: 5677 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5677 PfoI T'CCnGG_A Cuts at: 2313 2313 Size: 5677 PmeI GTTT'AAAC Cuts at: 2981 2981 Size: 5677 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3746 3778 5259 5291 3746 Size: 32 1481 32 4132 Fragments arranged by size: 4132 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2764 2898 2764 Size: 134 5543 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5677 PsiI TTA'TAA Cuts at: 3247 3247 Size: 5677 PspOMI G'GGCC_C Cuts at: 2365 2365 Size: 5677 PstI C_TGCA'G Cuts at: 830 2772 3029 830 Size: 1942 257 3478 Fragments arranged by size: 3478 1942 257 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5677 PvuII CAG'CTG Cuts at: 2602 2773 2810 2602 Size: 171 37 5469 Fragments arranged by size: 5469 171 37 SacI G_AGCT'C Cuts at: 2352 2352 Size: 5677 SalI G'TCGA_C Cuts at: 1936 3019 1936 Size: 1083 4594 SanDI GG'GwC_CC Cuts at: 2898 2898 Size: 5677 SbfI CC_TGCA'GG Cuts at: 3029 3029 Size: 5677 ScaI AGT'ACT Cuts at: 3925 5637 3925 Size: 1712 3965 SfcI C'TryA_G Cuts at: 826 1045 2768 2785 3025 4160 4841 5032 826 Size: 219 1723 17 240 1135 681 191 1471 Fragments arranged by size: 1723 1471 1135 681 240 219 191 17 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5677 SmaI CCC'GGG Cuts at: 1872 3004 1872 Size: 1132 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3781 4682 4944 820 Size: 197 926 1838 901 262 1553 Fragments arranged by size: 1838 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3468 493 Size: 2975 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5677 SphI G_CATG'C Cuts at: 1132 3035 1132 Size: 1903 3774 SspI AAT'ATT Cuts at: 5 52 3601 5 Size: 47 3549 2081 Fragments arranged by size: 3549 2081 47 StuI AGG'CCT Cuts at: 1517 2957 1517 Size: 1440 4237 StyI C'CwwG_G Cuts at: 513 1065 2004 2527 2736 2778 2865 3116 513 Size: 552 939 523 209 42 87 251 3074 Fragments arranged by size: 3074 939 552 523 251 209 87 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2112 3704 3721 3874 4059 4504 325 Size: 1255 532 1592 17 153 185 445 1498 Fragments arranged by size: 1592 1498 1255 532 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3923 5635 96 Size: 274 80 33 51 1443 1946 1712 138 Fragments arranged by size: 1946 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5637 5669 5637 Size: 32 5645 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5677 XbaI T'CTAG_A Cuts at: 3013 3013 Size: 5677 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5677 XmaI C'CCGG_G Cuts at: 1870 3002 1870 Size: 1132 4545 XmnI GAAnn'nnTTC Cuts at: 3806 3806 Size: 5677 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3485 276 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI