Restriction map for pFN21AB8114
 (Circular) (Six-base) MAPSORT of: pFN21AB8114.seq  Check: 4074  from: 1  to: 5677



pFN21AB8114  5677 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:43  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2816    2816 
   Size:       5677
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3487     278 
   Size:         53      83     186     936    1951    2468

  Fragments arranged by size: 

                2468    1951     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3020    1937 
   Size:       1083    4594
 
Acc65I G'GTAC_C

Cuts at:   2998    2998 
   Size:       5677
 
AclI AA'CG_TT

Cuts at:   1563    3804    4177    1563 
   Size:       2241     373    3063

  Fragments arranged by size: 

                3063    2241     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2366    2458    2624    3740    5124    5392    1632 
   Size:        734      92     166    1116    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1116     734     268     166      92
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5480
 
AflIII A'CryG_T

Cuts at:   1203    4576    5553    1203 
   Size:       3373     977    1327

  Fragments arranged by size: 

                3373    1327     977
 
AgeI A'CCGG_T

Cuts at:   4508    4508 
   Size:       5677
 
AhdI GACnn_n'nnGTC

Cuts at:   4406    4406 
   Size:       5677
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5677
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2739    1184 
   Size:       1555    4122
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2378    2410    2378 
   Size:         32    5645
 
AlwNI CAG_nnn'CTG

Cuts at:   2237    2258    3420    4992    2237 
   Size:         21    1162    1572    2922

  Fragments arranged by size: 

                2922    1572    1162      21
 
ApaI G_GGCC'C

Cuts at:   2369    2369 
   Size:       5677
 
ApaLI G'TGCA_C

Cuts at:   3734    4890    3734 
   Size:       1156    4521
 
ApoI r'AATT_y

Cuts at:   1493    2138    2318    2986    3212    3364    5473    1493 
   Size:        645     180     668     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     668     645     226     180     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4231     160 
   Size:        872    3199    1606

  Fragments arranged by size: 

                3199    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5677
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2247    2300    3002    1870 
   Size:         73     304      53     702    4545

  Fragments arranged by size: 

                4545     702     304      73      53
 
BamHI G'GATC_C

Cuts at:   3007    3374    3007 
   Size:        367    5310
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2094    2998    4453     618 
   Size:        325     217     627     307     904    1455    1842

  Fragments arranged by size: 

                1842    1455     904     627     325     307     217
 
BanII G_rGCy'C

Cuts at:   1391    2352    2369    2996    1391 
   Size:        961      17     627    4072

  Fragments arranged by size: 

                4072     961     627      17
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2302     928 
   Size:        971     403    4303

  Fragments arranged by size: 

                4303     971     403
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3868    3902    1903 
   Size:         34    1931      34    3678

  Fragments arranged by size: 

                3678    1931      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3569    4779    3569 
   Size:       1210    4467
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5677
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4288     136 
   Size:        107     122      71     631     605    2616    1525

  Fragments arranged by size: 

                2616    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5672    5672 
   Size:       5677
 
BlpI GC'TnA_GC

Cuts at:   2657    3094    2657 
   Size:        437    5240
 
Bme1580I G_kGCm'C

Cuts at:   1790    2369    2445    2705    3738    4894    1790 
   Size:        579      76     260    1033    1156    2573

  Fragments arranged by size: 

                2573    1156    1033     579     260      76
 
BmgBI CAC'GTC

Cuts at:   1318    2032    1318 
   Size:        714    4963
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2654    3408    4366 
   Size:        416      53      83     399      24    1237     754     958

Cuts at:   4366     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1237     958     754     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5677
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2974    2974 
   Size:       5677
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2072    4337    1239 
   Size:        833    2265    2579

  Fragments arranged by size: 

                2579    2265     833
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3802    4667    4965    3802 
   Size:        865     298    4514

  Fragments arranged by size: 

                4514     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2328    4340     882 
   Size:        617     829    2012    2219

  Fragments arranged by size: 

                2219    2012     829     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3468     493 
   Size:        711    2264    2702

  Fragments arranged by size: 

                2702    2264     711
 
BsaBI GATnn'nnATC

Cuts at:   5671    5671 
   Size:       5677
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2533    3484    3866    4501 
   Size:         53      83     186     936    1000     951     382     635

Cuts at:   4501     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1000     951     936     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   4110    4508    4782    4929    5340    4110 
   Size:        398     274     147     411    4447

  Fragments arranged by size: 

                4447     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5452

  Fragments arranged by size: 

                5452     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2318    2446    2449    2603    2963    1186 
   Size:       1132     128       3     154     360    3900

  Fragments arranged by size: 

                3900    1132     360     154     128       3
 
BseYI C'CCAG_C

Cuts at:   2810    2871    4880    5376    2810 
   Size:         61    2009     496    3111

  Fragments arranged by size: 

                3111    2009     496      61
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5104
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2203    3127    3888    4916    1540 
   Size:        275     186     202     924     761    1028    2301

  Fragments arranged by size: 

                2301    1028     924     761     275     202     186
 
BsiHKAI G_wGCw'C

Cuts at:   2352    3738    3823    4894    2352 
   Size:       1386      85    1071    3135

  Fragments arranged by size: 

                3135    1386    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3188    3281    3188 
   Size:         93    5584
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2352    2369    2445    2705    2996    3738    3823 
   Size:        399     562      17      76     260     291     742      85

Cuts at:   3823    4894    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     562     399     291     260      85
                  76      17
 
BspEI T'CCGG_A

Cuts at:   5340    5340 
   Size:       5677
 
BspHI T'CATG_A

Cuts at:   3566    5296    3566 
   Size:       1730    3947
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2775    2816    3032     844 
   Size:       1931      41     216    3489

  Fragments arranged by size: 

                3489    1931     216      41
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2575    3564    1394 
   Size:        429     752     989    3507

  Fragments arranged by size: 

                3507     989     752     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4172    4346      65 
   Size:       1183    2924     174    1396

  Fragments arranged by size: 

                2924    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4321    4508    1700 
   Size:        110    2511     187    2869

  Fragments arranged by size: 

                2869    2511     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5677
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5677
 
BssSI C'ACGA_G

Cuts at:   3737    4749    3737 
   Size:       1012    4665
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5677
 
BstBI TT'CG_AA

Cuts at:   4481    4481 
   Size:       5677
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5677
 
BstYI r'GATC_y

Cuts at:   1972    2022    2881    3007    3374    3759    3776    5228    5314 
   Size:         50     859     126     367     385      17    1452      86

Cuts at:   5314    5326    5672    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     859     385     367     346     126      86
                  50      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2778     513 
   Size:        552     939     774    3412

  Fragments arranged by size: 

                3412     939     774     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4938

  Fragments arranged by size: 

                4938     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2269    3193    3987    4007    2269 
   Size:        924     794      20    3939

  Fragments arranged by size: 

                3939     924     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5677
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5642
 
DraI TTT'AAA

Cuts at:   2981    2981 
   Size:       5677
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4684     809 
   Size:        507    3368    1802

  Fragments arranged by size: 

                3368    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2151    2209    2775 
   Size:         54    1425      50     275      18     321      58     566

Cuts at:   2775    3124    4013    5642       8 
   Size:        349     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     566     349     321     275      58
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3124    1537 
   Size:        275    1312    4090

  Fragments arranged by size: 

                4090    1312     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2431    3607    1353 
   Size:       1078    1176    3423

  Fragments arranged by size: 

                3423    1176    1078
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4264    4638    4784     387 
   Size:       1064     138    2675     374     146    1280

  Fragments arranged by size: 

                2675    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2350    2350 
   Size:       5677
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2072    2366    2458    2624    3740    4337    5124 
   Size:        393     440     294      92     166    1116     597     787

Cuts at:   5124    5392    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1116     787     597     440     393     294     268
                 166      92
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2753    2753 
   Size:       5677
 
EcoO109I rG'GnC_Cy

Cuts at:   2366    2408    2764    2898    2366 
   Size:         42     356     134    5145

  Fragments arranged by size: 

                5145     356     134      42
 
EcoRI G'AATT_C

Cuts at:   2318    2986    3364    2318 
   Size:        668     378    4631

  Fragments arranged by size: 

                4631     668     378
 
FspI TGC'GCA

Cuts at:   4183    4183 
   Size:       5677
 
HaeII r_GCGC'y

Cuts at:   1678    4824    1678 
   Size:       3146    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2441    2473    2523    2555    4318 
   Size:         32     135      32     869      32      50      32    1763

Cuts at:   4318    4350    4392    4424    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1763     869     135      50      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3021    3267     669 
   Size:        988     281    1083     246    3079

  Fragments arranged by size: 

                3079    1083     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5677
 
HpaI GTT'AAC

Cuts at:   3267    3267 
   Size:       5677
 
KpnI G_GTAC'C

Cuts at:   3002    3002 
   Size:       5677
 
MfeI C'AATT_G

Cuts at:   3276    3276 
   Size:       5677
 
MluI A'CGCG_T

Cuts at:   5553    5553 
   Size:       5677
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4791    4975    1305 
   Size:       3486     184    2007

  Fragments arranged by size: 

                3486    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2777      10 
   Size:         54    1425     343     945    2910

  Fragments arranged by size: 

                2910    1425     945     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3635    3994    4153     518 
   Size:        648    2469     359     159    2042

  Fragments arranged by size: 

                2469    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2051    2602    2773    2810    3093    3770    4918    5163 
   Size:        445     551     171      37     283     677    1148     245

Cuts at:   5163    1606 
   Size:       2120

  Fragments arranged by size: 

                2120    1148     677     551     445     283     245     171
                  37
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2778     513 
   Size:        552     939     774    3412

  Fragments arranged by size: 

                3412     939     774     552
 
NdeI CA'TA_TG

Cuts at:    387    2161     387 
   Size:       1774    3903
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5677
 
NotI GC'GGCC_GC

Cuts at:   3124    3124 
   Size:       5677
 
NruI TCG'CGA

Cuts at:   2667    2667 
   Size:       5677
 
NspI r_CATG'y

Cuts at:   1132    1229    3035    4580    1132 
   Size:         97    1806    1545    2229

  Fragments arranged by size: 

                2229    1806    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    2438    1348 
   Size:       1090    4587
 
PciI A'CATG_T

Cuts at:   4576    4576 
   Size:       5677
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5677
 
PfoI T'CCnGG_A

Cuts at:   2313    2313 
   Size:       5677
 
PmeI GTTT'AAAC

Cuts at:   2981    2981 
   Size:       5677
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3746    3778    5259    5291    3746 
   Size:         32    1481      32    4132

  Fragments arranged by size: 

                4132    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2764    2898    2764 
   Size:        134    5543
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5677
 
PsiI TTA'TAA

Cuts at:   3247    3247 
   Size:       5677
 
PspOMI G'GGCC_C

Cuts at:   2365    2365 
   Size:       5677
 
PstI C_TGCA'G

Cuts at:    830    2772    3029     830 
   Size:       1942     257    3478

  Fragments arranged by size: 

                3478    1942     257
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5677
 
PvuII CAG'CTG

Cuts at:   2602    2773    2810    2602 
   Size:        171      37    5469

  Fragments arranged by size: 

                5469     171      37
 
SacI G_AGCT'C

Cuts at:   2352    2352 
   Size:       5677
 
SalI G'TCGA_C

Cuts at:   1936    3019    1936 
   Size:       1083    4594
 
SanDI GG'GwC_CC

Cuts at:   2898    2898 
   Size:       5677
 
SbfI CC_TGCA'GG

Cuts at:   3029    3029 
   Size:       5677
 
ScaI AGT'ACT

Cuts at:   3925    5637    3925 
   Size:       1712    3965
 
SfcI C'TryA_G

Cuts at:    826    1045    2768    2785    3025    4160    4841    5032     826 
   Size:        219    1723      17     240    1135     681     191    1471

  Fragments arranged by size: 

                1723    1471    1135     681     240     219     191      17
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5677
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5677
 
SmaI CCC'GGG

Cuts at:   1872    3004    1872 
   Size:       1132    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3781    4682    4944     820 
   Size:        197     926    1838     901     262    1553

  Fragments arranged by size: 

                1838    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3468     493 
   Size:       2975    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5677
 
SphI G_CATG'C

Cuts at:   1132    3035    1132 
   Size:       1903    3774
 
SspI AAT'ATT

Cuts at:      5      52    3601       5 
   Size:         47    3549    2081

  Fragments arranged by size: 

                3549    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2957    1517 
   Size:       1440    4237
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2527    2736    2778    2865    3116     513 
   Size:        552     939     523     209      42      87     251    3074

  Fragments arranged by size: 

                3074     939     552     523     251     209      87      42
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2112    3704    3721    3874    4059    4504     325 
   Size:       1255     532    1592      17     153     185     445    1498

  Fragments arranged by size: 

                1592    1498    1255     532     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3923    5635      96 
   Size:        274      80      33      51    1443    1946    1712     138

  Fragments arranged by size: 

                1946    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5637    5669    5637 
   Size:         32    5645
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5677
 
XbaI T'CTAG_A

Cuts at:   3013    3013 
   Size:       5677
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5677
 
XmaI C'CCGG_G

Cuts at:   1870    3002    1870 
   Size:       1132    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3806    3806 
   Size:       5677
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3485     276 
   Size:         53      83     186     936    1951    2468

  Fragments arranged by size: 

                2468    1951     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI
     NruI     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI
    PpuMI    PshAI     PsiI   PspOMI     PstI     PvuI    PvuII     SacI
     SalI    SanDI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AscI    AvrII     BaeI     BbeI    BbvCI    BfrBI   Bpu10I
    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII    EcoRV     FalI
     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NsiI     PacI
     PmlI    PspXI     PsrI    RsrII    SacII     SapI    SexAI     SfiI
     SfoI     SrfI     SwaI     XcmI
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